{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T04:12:17Z","timestamp":1793333537144,"version":"4.5.0"},"reference-count":39,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T00:00:00Z","timestamp":1583193600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China (No. 31572393), China Agriculture Research System (CARS42) and the Agricultural Science and Technology Innovation Program (ASTIPIAS04) of the Chinese Academy of Agricultural Sciences","award":["No. 31572393"],"award-info":[{"award-number":["No. 31572393"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Poultry diseases pose a large threat to poultry production. Selection to improve immune traits is a feasible way to prevent and control avian diseases. The objective of this study was to investigate the efficiency of estimation of genetic parameters for antibody response to avian influenza virus (Ab-AIV), antibody response to Newcastle disease virus (Ab-NDV), sheep red blood cell antibody titer (SRBC), the ratio of heterophils to lymphocytes (H\/L), immunoglobulin G (IgG), the spleen immune index (SII), thymus immune index (TII), thymus weight at 100 d (TW) and the spleen weight at 100 d (SW) in Beijing oil chickens, by using the best linear unbiased prediction (BLUP) method and genomic best linear unbiased prediction (GBLUP) method. The phenotypic data used in the two methods were the same and were from 519 individuals. With the BLUP model, Ab-AIV, Ab-NDV, SRBC, H\/L, IgG, TII, and TW had low heritability ranging from 0.000 to 0.281, whereas SII and SW had high heritability of 0.631 and 0.573. With the GBLUP model, all individuals were genotyped with Illumina 60K SNP chips, and Ab-AIV, Ab-NDV, SRBC, H\/L and IgG had low heritability ranging from 0.000 to 0.266, whereas SII, TII, TW and SW had moderate heritability ranging from 0.300 to 0.472. We compared the prediction accuracy obtained from BLUP and GBLUP through 50 time 5-fold cross-validation (CV), and the results indicated that BLUP provided a slightly higher accuracy of prediction than GBLUP in this population.<\/jats:p>","DOI":"10.3390\/ani10030419","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T13:06:23Z","timestamp":1583240783000},"page":"419","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Comparison of the Efficiency of BLUP and GBLUP in Genomic Prediction of Immune Traits in Chickens"],"prefix":"10.3390","volume":"10","author":[{"given":"Jin","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"State Key Laboratory of Animal Nutrition, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"State Key Laboratory of Animal Nutrition, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghe","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"State Key Laboratory of Animal Nutrition, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"State Key Laboratory of Animal Nutrition, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wen","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"State Key Laboratory of Animal Nutrition, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiping","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"State Key Laboratory of Animal Nutrition, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"842","DOI":"10.3382\/ps.2008-00289","article-title":"Avian influenza infection in birds: A challenge and opportunity for the poultry veterinarian","volume":"88","author":"Capua","year":"2009","journal-title":"Poult. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.2460\/javma.2003.222.1534","article-title":"Avian influenza and Newcastle disease","volume":"222","author":"Swayne","year":"2005","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.3382\/ps.2009-00556","article-title":"The effects of control measures on the economic burden associated with epidemics of avian influenza in Italy","volume":"89","author":"Sartore","year":"2010","journal-title":"Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1023\/B:TROP.0000049274.28640.d7","article-title":"Haemolytic Complement Activity and Humoral Immune Responses to Sheep Red Blood Cells in Indigenous Chickens and in Eight German Dahlem Red Chicken Lines with Different Combinations of Major Genes (dwarf, naked neck and frizzled) of Tropical Interest","volume":"37","author":"Baelmans","year":"2005","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"205","DOI":"10.3382\/ps.0590205","article-title":"Production and persistence of antibodies in chickens to sheep erythrocytes. 2. Resistance to infectious diseases","volume":"59","author":"Gross","year":"1980","journal-title":"Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1093\/ps\/79.4.519","article-title":"Influence of genetic selection for antibody production against sheep blood cells on energy metabolism in laying hens","volume":"79","author":"Mashaly","year":"2000","journal-title":"Poult. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1093\/ps\/83.6.853","article-title":"Quantitative trait loci for body weight in layers differ from quantitative trait loci specific for antibody responses to sheep red blood cells","volume":"83","author":"Siwek","year":"2004","journal-title":"Poult. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.3382\/ps.2009-00064","article-title":"Genetic parameters for levels of natural antibodies in chicken lines divergently selected for specific antibody response","volume":"88","author":"Wijga","year":"2009","journal-title":"Poult. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1007\/s00360-010-0548-9","article-title":"Leucocyte profiles and H\/L ratios in chicks of Red-tailed Tropicbirds reflect the ontogeny of the immune system","volume":"181","author":"Dehnhard","year":"2011","journal-title":"J. Comp. Physiol. B."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1080\/00071660600829118","article-title":"Association between heterophil\/lymphocyte ratio, a marker of \u2019resistance\u2019 to stress, and some production and fitness traits in chickens","volume":"47","year":"2006","journal-title":"Br. Poult. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1080\/00071668.2015.1008993","article-title":"H\/L ratio as a measurement of stress in laying hens\u2014methodology and reliability","volume":"56","author":"Lentfer","year":"2015","journal-title":"Br. Poult. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4414","DOI":"10.3168\/jds.2007-0980","article-title":"Efficient Methods to Compute Genomic Predictions","volume":"91","author":"Vanraden","year":"2008","journal-title":"J. Dairy Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1534\/genetics.116.189795","article-title":"Meuwissen et al. on Genomic Selection","volume":"203","author":"Koning","year":"2016","journal-title":"Genetics"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2270","DOI":"10.3382\/ps.2013-03085","article-title":"Pedigree and genomic analyses of feed consumption and residual feed intake in laying hens","volume":"92","author":"Wolc","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1297-9686-43-23","article-title":"Persistence of accuracy of genomic estimated breeding values over generations in layer chickens","volume":"43","author":"Wolc","year":"2011","journal-title":"Genet. Sel. Evol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s12711-015-0133-5","article-title":"Response and inbreeding from a genomic selection experiment in layer chickens","volume":"47","author":"Wolc","year":"2015","journal-title":"Genet. Sel. Evol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1146\/annurev-animal-031412-103705","article-title":"Accelerating Improvement of Livestock with Genomic Selection","volume":"1","author":"Meuwissen","year":"2013","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1038\/nrg2575","article-title":"Mapping genes for complex traits in domestic animals and their use in breeding programmes","volume":"10","author":"Goddard","year":"2009","journal-title":"Nat. Rev. Genet."},{"key":"ref_19","first-page":"725","article-title":"[SNP assisted total genomic selection as a possible accelerator of traditional selection]","volume":"45","author":"Smaragdov","year":"2009","journal-title":"Genetika"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Liu, T., Luo, C., Wang, J., Ma, J., Shu, D., Lund, M.S., Su, G., Qu, H., and Zhang, Q. (2017). Assessment of the genomic prediction accuracy for feed efficiency traits in meat-type chickens. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0173620"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"730","DOI":"10.3389\/fgene.2018.00730","article-title":"Using Different Single-Step Strategies to Improve the Efficiency of Genomic Prediction on Body Measurement Traits in Pig","volume":"9","author":"Song","year":"2019","journal-title":"Front. Genet."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/j.1750-2659.2012.00391.x","article-title":"Serological diagnosis of avian influenza in poultry: Is the haemagglutination inhibition test really the \u2019gold standard\u2019?","volume":"7","author":"Comin","year":"2012","journal-title":"Influenza Other Respir. Viruses"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0165-2427(97)00151-7","article-title":"Testing of bovine sera by ELISA for IgG, IgM and IgA rheumatoid factors","volume":"61","author":"Graham","year":"1998","journal-title":"Vet. Immunol. Immunop."},{"key":"ref_24","first-page":"221","article-title":"The influence of different beak trimming age on performance, H-L ratio and antibody production to SRBC in laying hens","volume":"41","author":"Ender","year":"2008","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1080\/03079459708419218","article-title":"Comparison of enzyme-linked immunosorbent assay and haemagglutination inhibition test for the detection of antibodies against Newcastle disease virus in ostriches (Struthio camelus)","volume":"26","author":"Cadman","year":"1997","journal-title":"Avian Pathol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"423","DOI":"10.2307\/2529430","article-title":"Best Linear Unbiased Estimation and Prediction under a Selection Model","volume":"31","author":"Henderson","year":"1975","journal-title":"Biometrics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"9061","DOI":"10.3168\/jds.2017-12604","article-title":"The genetic and biological basis of feed efficiency in mid-lactation Holstein dairy cows","volume":"100","author":"Hardie","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.3168\/jds.2009-2192","article-title":"Preliminary investigation on reliability of genomic estimated breeding values in the Danish Holstein population","volume":"93","author":"Su","year":"2010","journal-title":"J. Dairy Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1534\/genetics.108.088575","article-title":"Performance of genomic selection in mice","volume":"180","author":"Manfredi","year":"2008","journal-title":"Genetics"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Liu, T., Qu, H., Luo, C., Li, X., Shu, D., Lund, M.S., Su, G., and Krammer, F. (2014). Genomic selection for the improvement of antibody response to Newcastle disease and avian influenza virus in chickens. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0112685"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v028.i05","article-title":"Building predictive models in R using the caret package","volume":"28","author":"Kuhn","year":"2008","journal-title":"J. Stat. Softw."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1080\/00071669708418025","article-title":"Antibody transmitting ability of hens from lines of chickens differing in response to SRBC antigen","volume":"38","author":"Dunnington","year":"1997","journal-title":"Br. Poult. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1080\/0007166022000004408","article-title":"Genetic resistance to Salmonella typhimurium in two lines of chickens selected as resistant and sensitive on the basis of heterophil\/lymphocyte ratio","volume":"43","author":"Raof","year":"2002","journal-title":"Br. Poult. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.tpb.2015.08.006","article-title":"Pedigrees or markers: Which are better in estimating relatedness and inbreeding coefficient?","volume":"107","author":"Wang","year":"2016","journal-title":"Theoretical Population Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3434","DOI":"10.1111\/mec.12827","article-title":"Estimating quantitative genetic parameters in wild populations: A comparison of pedigree and genomic approaches","volume":"23","author":"Ellis","year":"2014","journal-title":"Mol. Ecol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e1002051","DOI":"10.1371\/journal.pgen.1002051","article-title":"Beyond Missing Heritability: Prediction of Complex Traits","volume":"7","author":"Makowsky","year":"2011","journal-title":"PloS Genet."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1111\/j.1439-0388.2007.00700.x","article-title":"Comparison of genomic and traditional BLUP-estimated breeding value accuracy and selection response under alternative trait and genomic parameters","volume":"124","author":"Muir","year":"2015","journal-title":"J. Anim. Breed Genet."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5248","DOI":"10.3168\/jds.2009-2092","article-title":"Predictive ability of direct genomic values for lifetime net merit of Holstein sires using selected subsets of single nucleotide polymorphism markers","volume":"92","author":"Weigel","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"5942","DOI":"10.3168\/jds.2010-3335","article-title":"Predictive ability of subsets of single nucleotide polymorphisms with and without parent average in US Holsteins","volume":"93","author":"Vazquez","year":"2010","journal-title":"J. Dairy Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/3\/419\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:03:31Z","timestamp":1760173411000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/3\/419"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,3]]},"references-count":39,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,3]]}},"alternative-id":["ani10030419"],"URL":"https:\/\/doi.org\/10.3390\/ani10030419","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,3]]}}}