{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:25:43Z","timestamp":1790713543420,"version":"4.1.0"},"reference-count":51,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,3,8]],"date-time":"2020-03-08T00:00:00Z","timestamp":1583625600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2018\/02\/X\/NZ6\/00840"],"award-info":[{"award-number":["2018\/02\/X\/NZ6\/00840"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["APVV-17-0017"],"award-info":[{"award-number":["APVV-17-0017"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Snake venom is an extremely interesting natural mixture of proteins and peptides, characterized by both high diversity and high pharmacological potential. Much attention has been paid to the study of venom composition of different species and also detailed analysis of the properties of individual components. Since proteins and peptides are the active ingredients in venom, rapidly developing proteomic techniques are used to analyze them. During such analyses, one of the routine operations is to measure the protein concentration in the sample. The aim of this study was to compare five methods used to measure protein content in venoms of two snake species: the Viperids representative, Agkistrodon contortrix, and the Elapids representative, Naja ashei. The study showed that for A. contortrix venom, the concentration of venom protein measured by four methods is very similar and only the NanoDrop method clearly stands out from the rest. However, in the case of N. ashei venom, each technique yields significantly different results. We hope that this report will help to draw attention to the problem of measuring protein concentration, especially in such a complex mixture as animal venoms.<\/jats:p>","DOI":"10.3390\/ani10030448","type":"journal-article","created":{"date-parts":[[2020,3,9]],"date-time":"2020-03-09T05:37:34Z","timestamp":1583732254000},"page":"448","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Comparison of Methods for Measuring Protein Concentration in Venom Samples"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1907-9399","authenticated-orcid":false,"given":"Aleksandra","family":"Bocian","sequence":"first","affiliation":[{"name":"Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"S\u0142awek","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Jaromin","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2723-3691","authenticated-orcid":false,"given":"Konrad K.","family":"Hus","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justyna","family":"Buczkowicz","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawid","family":"\u0141ysiak","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Petr\u00edlla","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy, Komensk\u00e9ho 73, 041 81 Kosice, Slovakia"},{"name":"Zoological Department, Zoological Garden Ko\u0161ice, \u0160irok\u00e1 31, 040 06 Ko\u0161ice-Kave\u010dany, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Petrillova","sequence":"additional","affiliation":[{"name":"Department of General Education Subjects, University of Veterinary Medicine and Pharmacy, Komensk\u00e9ho 73, 041 81 Kosice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaroslav","family":"Leg\u00e1th","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Rzeszow University of Technology, 35-959 Rzesz\u00f3w, Poland"},{"name":"Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komensk\u00e9ho 73, 041 81 Kosice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1016\/0041-0101(91)90116-9","article-title":"Snake venom variability: methods of study, results and interpretation","volume":"29","author":"Chippaux","year":"1991","journal-title":"Toxicon"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.tree.2012.10.020","article-title":"Complex cocktails: The evolutionary novelty of venoms","volume":"28","author":"Casewell","year":"2013","journal-title":"Trends Ecol. 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