{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T17:00:41Z","timestamp":1780592441037,"version":"3.54.1"},"reference-count":37,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T00:00:00Z","timestamp":1585094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772631, 31702141"],"award-info":[{"award-number":["31772631, 31702141"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the National Key Research and Development Program of China","award":["2018YFD0501903"],"award-info":[{"award-number":["2018YFD0501903"]}]},{"name":"Key Research Program of the Chinese Academy of Sciences","award":["KFZD-SW-219"],"award-info":[{"award-number":["KFZD-SW-219"]}]},{"name":"CAS Science and Technology Service Network Initiative","award":["KFJ-STS-ZDTP-075"],"award-info":[{"award-number":["KFJ-STS-ZDTP-075"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Weaning usually leads to stress in livestock, which has a negative impact on their growth and development. Research on oxidative stress and inflammation induced by weaning has not been reported in goats. Here, we focused on oxidative stress profile and inflammation status of the lower gut (jejunum, ileum, and colon) of goats. First, we illustrated the status of antioxidant activity and inflammation in the intestine of young goats on pre-(2 weeks postnatal, 2 wkpn) or post-(11 wkpn, weaning at day 45 postnatal)-weaned period of young goats. Malondialdehyde (MDA) was higher (p &lt; 0.0001) in jejunum and ileum of the young goats in 11 wkpn than that in 2 wkpn, whereas superoxide dismutase (SOD) activity was lower (p = 0.012) in the lower gut of the young goats with 11 wkpn than that in 2 wkpn. Furthermore, we intended to explore the protective influence of a probiotic additive (Lactobacillus plantarum (LAC) P-8, 10 g\/d) and a prebiotic additive (Sangrovit\u00ae, Macleaya cordata (MAC) extract 3.75% w\/w premix, 0.3 g\/d) on intestinal oxidative stress and inflammation status of early-weaned young goats (average weights of 5.63 \u00b1 0.30 kg, weaned on d 45 postnatal). We observed that LAC reduced MDA in jejunum and ileum (p &lt; 0.0001), increased SOD activity in ileum (p &lt; 0.01), and increased glutathione peroxidase (GSH-Px) activity in jejunum (p &lt; 0.05). Similarly, MAC reduced MDA contents (p &lt; 0.0001), increased SOD activities (p &lt; 0.01) in both of ileum and jejunum, and increased GSH-Px activity (p &lt; 0.05) in jejunum. However, there were no differences in feed intake, average daily gain, inflammation parameters (interleukin 2 and interleukin 6), and colon oxidative stress profile (MDA, SOD, or GSH-Px) among treatments. These results provide evidence that weaning induces oxidative damage in the lower gut of young goats, and the oxidative damage in the small intestine can be reduced by adding the addition of LAC or MAC in diets depending on the region of the lower gut.<\/jats:p>","DOI":"10.3390\/ani10040548","type":"journal-article","created":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T13:10:47Z","timestamp":1585141847000},"page":"548","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Supplementation of Lactobacillus plantarum or Macleaya cordata Extract Alleviates Oxidative Damage Induced by Weaning in the Lower Gut of Young Goats"],"prefix":"10.3390","volume":"10","author":[{"given":"Kai","family":"Chen","sequence":"first","affiliation":[{"name":"CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, Hunan, China"},{"name":"College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6227-4494","authenticated-orcid":false,"given":"Yong","family":"Liu","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, Hunan, China"},{"name":"Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha 410128, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Cheng","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, Hunan, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0035-6819","authenticated-orcid":false,"given":"Qiongxian","family":"Yan","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, Hunan, China"},{"name":"Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha 410128, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanshe","family":"Zhou","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, Hunan, China"},{"name":"Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha 410128, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4802-3506","authenticated-orcid":false,"given":"Zhixiong","family":"He","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, Hunan, China"},{"name":"Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha 410128, Hunan, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianguo","family":"Zeng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, Hunan, China"},{"name":"Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha 410128, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9289-4405","authenticated-orcid":false,"given":"Jianhua","family":"He","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, Hunan, China"},{"name":"Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha 410128, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiliang","family":"Tan","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, Hunan, China"},{"name":"Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha 410128, Hunan, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3168\/jds.2016-11399","article-title":"Effects of Saccharomyces cerevisiae fermentation products on dairy calves: Performance and health","volume":"100","author":"Alugongo","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1159\/000491967","article-title":"Effects of Dietary Serine Supplementation on Intestinal Integrity, Inflammation and Oxidative Status in Early-Weaned Piglets","volume":"48","author":"Zhou","year":"2018","journal-title":"Cell Physiol. Biochem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1155\/2016\/4768541","article-title":"Weaning Induced Hepatic Oxidative Stress, Apoptosis, and Aminotransferases through MAPK Signaling Pathways in Piglets","volume":"2016","author":"Luo","year":"2016","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1017\/S1751731116001397","article-title":"A carvacrol-thymol blend decreased intestinal oxidative stress and influenced selected microbes without changing the messenger RNA levels of tight junction proteins in jejunal mucosa of weaning piglets","volume":"11","author":"Wei","year":"2017","journal-title":"Animal"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-018-33649-8","article-title":"Weaning Stress Perturbs Gut Microbiome and Its Metabolic Profile in Piglets","volume":"8","author":"Li","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_6","first-page":"1766","article-title":"Antioxidant properties of lactobacillus and its protecting effects to oxidative stress caco-2 cells","volume":"24","author":"Wu","year":"2014","journal-title":"J. Anim. Plant Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0168-1605(01)00674-2","article-title":"Two antioxidative lactobacilli strains as promising probiotics","volume":"72","author":"Kullisaar","year":"2002","journal-title":"Int. J. Food Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.5713\/ajas.2012.12079","article-title":"Lactobacillus plantarum ZLP001: In vitro Assessment of Antioxidant Capacity and Effect on Growth Performance and Antioxidant Status in Weaning Piglets","volume":"25","author":"Wang","year":"2012","journal-title":"Asian Austral. J. Anim."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1556\/APhysiol.100.2013.1.9","article-title":"Lactobacillus plantarum 2142 prevents intestinal oxidative stress in optimized in vitro systems","volume":"100","author":"Szeker","year":"2013","journal-title":"Acta Physiol. Hung."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1128\/IAI.73.2.912-920.2005","article-title":"Probiotic Lactobacillus spp. diminish Helicobacter hepaticus-induced inflammatory bowel disease in interleukin-10-deficient mice","volume":"73","author":"Pena","year":"2005","journal-title":"Infect. Immun."},{"key":"ref_11","first-page":"E81","article-title":"Prophylactic Effect of Lactobacillus pentosus strain S-PT84 on Candida Infection and Gastric Inflammation in a Murine Gastrointestinal Candidiasis Model Errata","volume":"57","author":"Maekawa","year":"2016","journal-title":"Med. Mycol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"39","DOI":"10.12938\/bmfh.16-011","article-title":"Specific vaginal lactobacilli suppress the inflammation induced by lipopolysaccharide stimulation through downregulation of toll-like receptor 4 expression in human embryonic intestinal epithelial cells","volume":"36","author":"Tobita","year":"2017","journal-title":"Biosci. Microb. Food Health"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"299","DOI":"10.3920\/BM2017.0084","article-title":"Lactobacillus salivarius LA307 and Lactobacillus rhamnosus LA305 attenuate skin inflammation in mice","volume":"9","author":"Holowacz","year":"2018","journal-title":"Benef. Microbes"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/s40168-018-0440-5","article-title":"Predominant gut Lactobacillus murinus strain mediates anti-inflammaging effects in calorie-restricted mice","volume":"6","author":"Pan","year":"2018","journal-title":"Microbiome"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40168-018-0590-5","article-title":"The chicken gut metagenome and the modulatory effects of plant-derived benzylisoquinoline alkaloids","volume":"6","author":"Huang","year":"2018","journal-title":"Microbiome"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1080\/14786419.2015.1017490","article-title":"Chemical composition, antioxidant and antimicrobial activity of the essential oil from the leaves of Macleaya cordata (Willd) R. Br.","volume":"30","author":"Li","year":"2016","journal-title":"Nat. Prod. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1007\/s13213-011-0377-4","article-title":"In vitro screen of Lactobacillus plantarum as probiotic bacteria and their fermented characteristics in soymilk","volume":"62","author":"Bao","year":"2012","journal-title":"Ann. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1097\/00005373-199101000-00007","article-title":"Early measurement of systemic lipid-peroxidation products in the plasma of major blunt trauma patients","volume":"31","author":"Girotti","year":"1991","journal-title":"J. Trauma"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0076-6879(84)05013-8","article-title":"superoxide-dismutase assays","volume":"105","author":"Flohe","year":"1984","journal-title":"Methods Enzymol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1016\/0006-291X(76)90747-6","article-title":"Glutathione peroxidase activity in selenium-deficient rat liver","volume":"71","author":"Lawrence","year":"1976","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"535","DOI":"10.3389\/fphys.2017.00535","article-title":"The Effects of Weaning Methods on Gut Microbiota Composition and Horse Physiology","volume":"8","author":"Mach","year":"2017","journal-title":"Front. Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4319","DOI":"10.2527\/jas.2012-5088","article-title":"Effects of maternal protein or energy restriction during late gestation on antioxidant status of plasma and immune tissues in postnatal goats","volume":"90","author":"He","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s00204-008-0304-z","article-title":"Intracellular redox status and oxidative stress: Implications for cell proliferation, apoptosis, and carcinogenesis","volume":"82","author":"Mates","year":"2008","journal-title":"Arch. Toxicol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/s00204-009-0425-z","article-title":"Oxidative stress and apoptotic changes in primary cultures of rat proximal tubular cells exposed to lead","volume":"83","author":"Wang","year":"2009","journal-title":"Arch. Toxicol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.arr.2009.09.004","article-title":"Lipid peroxidation in relation to ageing and the role of endogenous aldehydes in diabetes and other age-related diseases","volume":"9","author":"Dmitriev","year":"2010","journal-title":"Ageing Res. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1111\/jcmm.12475","article-title":"Blood markers of oxidative stress predict weaning failure from mechanical ventilation","volume":"19","author":"Verona","year":"2015","journal-title":"J. Cell Mol. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.3389\/fmicb.2017.01655","article-title":"Shifts in Host Mucosal Innate Immune Function Are Associated with Ruminal Microbial Succession in Supplemental Feeding and Grazing Goats at Different Ages","volume":"8","author":"Jiao","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"90","DOI":"10.3389\/fmicb.2019.00090","article-title":"Lactobacillus plantarum PFM 105 promotes intestinal development through modulation of gut microbiota in weaning piglets","volume":"10","author":"Wang","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"272","DOI":"10.17221\/5\/2019-CJAS","article-title":"Effect of supplemental lactic acid bacteria on growth performance, glutathione turnover and aflatoxin B1 removal in lambs","volume":"64","author":"Wang","year":"2019","journal-title":"Czech J. Anim. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.3389\/fimmu.2018.01131","article-title":"Redox Role of Lactobacillus casei Shirota against the cellular damage induced by 2,2\u2019-Azobis (2-Amidinopropane) dihydrochloride-induced oxidative and inflammatory stress in enterocytes-like epithelial cells","volume":"9","author":"Finamore","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_31","first-page":"006","article-title":"Effects and immune responses of probiotic treatment in ruminants","volume":"12","author":"Raabis","year":"2018","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.livsci.2007.01.079","article-title":"Effects of Chinese herbal ultra-fine powder as a dietary additive on growth performance, serum metabolites and intestinal health in early-weaned piglets","volume":"108","author":"Kong","year":"2007","journal-title":"Livest. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1111\/jpn.12528","article-title":"Effects of different levels of sanguinarine on antioxidant indices, immunological responses, ileal microbial counts and jejunal morphology of laying hens fed diets with different levels of crude protein","volume":"101","author":"Bavarsadi","year":"2017","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1046\/j.1365-2672.2003.01850.x","article-title":"Probiotic properties of human lactobacilli strains to be used in the gastrointestinal tract","volume":"94","author":"Fernandez","year":"2003","journal-title":"J. Appl. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.fsi.2018.11.003","article-title":"Screening of intestinal probiotics and the effects of feeding probiotics on the growth, immune, digestive enzyme activity and intestinal flora of Litopenaeus vannamei","volume":"86","author":"Zuo","year":"2019","journal-title":"Fish Shellfish Immun."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"723","DOI":"10.2527\/jas.2008-1031","article-title":"Use of a commercial probiotic supplement in meat goats","volume":"87","author":"Whitley","year":"2009","journal-title":"J. Anim. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1111\/jpn.12878","article-title":"Effects of mannan-oligosaccharides and Lactobacillus acidophilus supplementation on growth performance, nutrient utilization and faecal characteristics in Murrah buffalo calves","volume":"102","author":"Sharma","year":"2018","journal-title":"J. Anim. Physiol. Anim. Nutr."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/4\/548\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:11:24Z","timestamp":1760173884000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/4\/548"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,25]]},"references-count":37,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["ani10040548"],"URL":"https:\/\/doi.org\/10.3390\/ani10040548","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,25]]}}}