{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T09:31:33Z","timestamp":1773048693020,"version":"3.50.1"},"reference-count":65,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Project","award":["2018 YFD0500600"],"award-info":[{"award-number":["2018 YFD0500600"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31802104"],"award-info":[{"award-number":["31802104"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Natural Science of Guangdong Province","award":["2017A030310096"],"award-info":[{"award-number":["2017A030310096"]}]},{"name":"the &quot;Twelve-Five&quot; National Science and Technology Support Program","award":["2014BAD13B02"],"award-info":[{"award-number":["2014BAD13B02"]}]},{"name":"China Agriculture Research System from the Ministry of Agriculture","award":["CARS-41-G10"],"award-info":[{"award-number":["CARS-41-G10"]}]},{"name":"Scientific and Technological Project from the Department of Science and Technology of Guangdong Province","award":["2017B020202003"],"award-info":[{"award-number":["2017B020202003"]}]},{"name":"Grant from Guangzhou Science Technology and Innovation Commission","award":["201804020091"],"award-info":[{"award-number":["201804020091"]}]},{"name":"Presidential Foundation of the Guangdong Academy of Agricultural Sciences, PR China","award":["201620, 201805, 201807B, 201809B and 201908"],"award-info":[{"award-number":["201620, 201805, 201807B, 201809B and 201908"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study investigated the influence of dietary supplementation with some antibiotic alternatives on growth performance, intestinal barrier, and immunity of lipopolysaccharide (LPS) challenged chicks. Wenshi females, aged 4 days, were allocated randomly into eight groups, each with six replicates of 20 birds (n = 120\/treatment), which received a basal diet supplemented with 0 (control), 0 (LPS), 200 mg\/kg aureomycin, 50 mg\/kg mushroom polysaccharide, 100 mg\/kg mushroom polysaccharide, 500 mg\/kg nano-copper, 300 mg\/kg copper loaded chitosan, and 500 mg\/kg lysozyme for 21 days. On day 18 and 20, the control birds were injected with 0.5 mL saline solution, the other treatments were injected with 0.5 mL saline containing 500 \u00b5g LPS\/kg body weight (BW). The results indicated that LPS treatment reduced the BW, average daily gain (ADG), and daily feed intake (ADFI) than the controls (p &lt; 0.05), and the antibiotic and the tested alternatives could not retrieve the normal BW, ADG, and ADFI. The tested additives reduced several negative effects of LPS; they reduced diamine oxidase activity and inflammatory mediators in plasma, jejunal mucosa, spleen and thymus, increased content of immunoglobulin in plasma and jejunal mucosa, and decreased gene expression of inducible nitric oxide synthase and Cyclooxygenase 2 in jejunal mucosa.<\/jats:p>","DOI":"10.3390\/ani10040594","type":"journal-article","created":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T05:52:30Z","timestamp":1585720350000},"page":"594","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Influence of Mushroom Polysaccharide, Nano-Copper, Copper Loaded Chitosan, and Lysozyme on Intestinal Barrier and Immunity of LPS-mediated Yellow-Feathered Chickens"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8539-1471","authenticated-orcid":false,"given":"Qiuli","family":"Fan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6141-1095","authenticated-orcid":false,"given":"K. F. M.","family":"Abouelezz","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"},{"name":"Department of Poultry Production, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4030-6760","authenticated-orcid":false,"given":"Long","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8258-6532","authenticated-orcid":false,"given":"Zhongyong","family":"Gou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2699-6304","authenticated-orcid":false,"given":"Yibing","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0515-8555","authenticated-orcid":false,"given":"Xiajing","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2927-6986","authenticated-orcid":false,"given":"Jinling","family":"Ye","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9251-306X","authenticated-orcid":false,"given":"Shouqun","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.imbio.2007.11.008","article-title":"Lipopolysaccharide sensing an important factor in the innate immune response to Gram-negative bacterial infections: Benefits and hazards of LPS hypersensitivity","volume":"213","author":"Freudenberg","year":"2008","journal-title":"Immunobiology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.cca.2006.05.045","article-title":"LTA and LPS mediated activation of protein kinases in the regulation of inflammatory cytokines expression in macrophages","volume":"374","author":"Su","year":"2006","journal-title":"Clin. 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