{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:35:57Z","timestamp":1777926957928,"version":"3.51.4"},"reference-count":52,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T00:00:00Z","timestamp":1586390400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Chagas disease (ChD) is considered an emerging disease in the USA and Europe. Trypanosoma cruzi genes encoding a trans-sialidase protein and an amastigote-specific glycoprotein were tested as vaccines in canine model. The aim for this study was determining the prophylactic effect of these genes in experimentally infected dogs by echocardiography evaluation to compare with our findings obtained by other techniques published previously. Low fractional-shortening values of non-vaccinated dogs suggested an impairment in general cardiac function. Low left ventricular ejection fraction values found in infected dogs suggested myocardial injury regardless of whether they were vaccinated. Low left ventricular diastolic\/systolic diameters suggested that progressive heart damage or heart dilation could be prevented by DNA vaccination. Systolic peak time was higher in non-vaccinated groups, increasing vulnerability to malignant arrhythmias and sudden death. High left ventricular volume suggested a decrease in wall thickness that might lead to increased size of the heart cavity, except in the pBCSP plasmid-vaccinated dogs. There was an echocardiographic evidence of left ventricular dilation and reduction in systolic function in experimental chagasic dogs. Echocardiography allowed a more complete follow-up of the pathological process in the living patient than with other techniques like electrocardiography, anatomopathology, and histopathology, being the method of choice for characterizing the clinical stages of ChD.<\/jats:p>","DOI":"10.3390\/ani10040648","type":"journal-article","created":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T14:42:03Z","timestamp":1586443323000},"page":"648","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Echocardiographic Findings in Canine Model of Chagas Disease Immunized with DNA Trypanosoma cruzi Genes"],"prefix":"10.3390","volume":"10","author":[{"given":"Olivia","family":"Rodr\u00edguez-Morales","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, National Institute of Cardiology \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco-Javier","family":"Rold\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Echocardiography, National Institute of Cardiology \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"Vargas-Barr\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Echocardiography, National Institute of Cardiology \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrique","family":"Parra-Ben\u00edtez","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, National Institute of Cardiology \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda de Lourdes","family":"Medina-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, National Institute of Cardiology \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilia","family":"Vergara-Bello","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, National Institute of Cardiology \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minerva","family":"Arce-Fonseca","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, National Institute of Cardiology \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,9]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (WHO) (2019, November 24). Weekly Epidemiological Record. Available online: https:\/\/www.who.int\/wer\/2015\/wer9006.pdf?ua=1."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.3389\/fcimb.2020.00027","article-title":"The influence of environmental cues on the development of Trypanosoma cruzi in Triatominae vector","volume":"10","author":"Melo","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Guhl, F. (2017). Geographical distribution of Chagas disease. Am. Trypanosom. Chagas Dis., 89\u2013112.","DOI":"10.1016\/B978-0-12-801029-7.00005-8"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1051\/vetres\/2009009","article-title":"Trypanosoma cruzi: Adaptation to its vectors and its hosts","volume":"40","author":"Noireau","year":"2009","journal-title":"Vet. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1016\/S0140-6736(10)60061-X","article-title":"Chagas disease","volume":"375","author":"Rassi","year":"2010","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1590\/S0074-02762009000900005","article-title":"Current epidemiological trends of Chagas disease in Latin America and future challenges: Epidemiology, surveillance, and health policies","volume":"104","author":"Moncayo","year":"2009","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1016\/S0300-8932(09)73071-4","article-title":"Actualizaci\u00f3n sobre la cardiopat\u00eda de la enfermedad de Chagas en el primer centenario de su descubrimiento","volume":"62","author":"Rassi","year":"2009","journal-title":"Rev. Esp. Cardiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1097\/CRD.0b013e31823efde2","article-title":"Chagas Heart Disease: Report on Recent Developments","volume":"20","author":"Machado","year":"2012","journal-title":"Cardiol. Rev."},{"key":"ref_9","first-page":"602","article-title":"Clinical and echocardiographic predictors of mortality in chagasic cardiomyopathy\u2013systematic review","volume":"102","year":"2014","journal-title":"Arq. Bras. Cardiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"593","DOI":"10.2147\/VHRM.S8355","article-title":"Current and emerging therapeutic options for the treatment of chronic chagasic cardiomyopathy","volume":"6","author":"Muratore","year":"2010","journal-title":"Vasc. Health Risk Manag."},{"key":"ref_11","first-page":"825","article-title":"Chagas\u2019 heart disease","volume":"20","author":"Hagar","year":"1995","journal-title":"Curr. Probl. Cardiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1053\/euje.2001.0076","article-title":"Regional diastolic dysfunction in chronic Chagas\u2019 heart disease","volume":"2","author":"Acquatella","year":"2001","journal-title":"Eur. J. Echocardiogr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1161\/CIRCULATIONAHA.106.627323","article-title":"Echocardiography in Chagas heart disease","volume":"115","author":"Acquatella","year":"2007","journal-title":"Circulation"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1136\/hrt.2003.018960","article-title":"Value of echocardiography for diagnosis and prognosis of chronic Chagas disease cardiomyopathy without heart failure","volume":"90","author":"Viotti","year":"2004","journal-title":"Heart"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.pcad.2009.11.008","article-title":"Chagas Cardiomyopathy-Where Do We Stand After a Hundred Years?","volume":"52","author":"Biolo","year":"2010","journal-title":"Progr. Cardiovasc. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1017\/S0031182006001259","article-title":"Domestic dogs and cats as sources of Trypanosoma cruzi infection in rural northwestern Argentina","volume":"134","author":"Cecere","year":"2007","journal-title":"Parasitology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1128\/CMR.00067-12","article-title":"Transmission and epidemiology of zoonotic protozoal disease of companion animals","volume":"26","author":"Esch","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"604","DOI":"10.3389\/fimmu.2017.00604","article-title":"Canine macrophage DH82 cell line as a model to study susceptibility to Trypanosoma cruzi infection","volume":"8","author":"Moraes","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.cvsm.2010.03.007","article-title":"Advanced techniques in echocardiography in small animals","volume":"40","author":"Chetboul","year":"2010","journal-title":"Vet. Clin. Small Anim."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1590\/S0074-02761992000100011","article-title":"Experimental Chagas\u2019 disease in dogs","volume":"87","author":"Chiari","year":"1992","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s00428-001-0542-4","article-title":"Immunohistochemical studies in acute and chronic canine chagasic cardiomyopathy","volume":"441","author":"Caliari","year":"2002","journal-title":"Virchows Arch."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.exppara.2008.08.001","article-title":"Trypanosoma cruzi: Immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes","volume":"120","author":"Carneiro","year":"2008","journal-title":"Exp. Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1590\/S1984-29612012000200015","article-title":"Hematological alterations during experimental canine infection by Trypanosoma cruzi","volume":"21","author":"Guedes","year":"2012","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2246","DOI":"10.1016\/j.vaccine.2013.03.005","article-title":"Preventive and therapeutic DNA vaccination partially protect dogs against an infectious challenge with Trypanosoma cruzi","volume":"31","author":"Dumonteil","year":"2013","journal-title":"Vaccine"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1590\/0074-02760140208","article-title":"The TcI and TcII Trypanosoma cruzi experimental infections induce distinct immune responses and cardiac fibrosis in dogs","volume":"109","author":"Duz","year":"2014","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1297-9716-45-6","article-title":"Frequency of IFNg-producing T cells correlates with seroreactivity and activated T cells during canine Trypanosoma cruzi infection","volume":"45","author":"Hartley","year":"2014","journal-title":"Vet. Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Aparicio-Burgos, J.E., Zepeda-Escobar, J.A., de Oca-Jimenez, R.M., Estrada-Franco, J.G., Barbabosa-Pliego, A., Ochoa-Garc\u00eda, L., Alejandre-Aguilar, R., Rivas, N., Pe\u00f1uelas-Rivas, G., and Val-Arreola, M. (2015). Immune protection against Trypanosoma cruzi induced by TcVac4 in a canine model. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003625"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1017\/S0031182016000822","article-title":"The TcTASV proteins are novel promising antigens to detect active Trypanosoma cruzi infection in dogs","volume":"143","author":"Ragone","year":"2016","journal-title":"Parasitology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.actatropica.2016.05.007","article-title":"Chagas cardiomyopathy: The potential effect of benznidazole treatment on diastolic dysfunction and cardiac damage in dogs chronically infected with Trypanosoma cruzi","volume":"161","author":"Santos","year":"2016","journal-title":"Acta Trop."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.actatropica.2016.12.015","article-title":"Myocarditis in different experimental models infected by Trypanosoma cruzi is correlated with the production of IgG1 isotype","volume":"167","author":"Caldas","year":"2017","journal-title":"Acta Trop."},{"key":"ref_31","first-page":"489758","article-title":"Experimental vaccines against Chagas disease: A journey through history","volume":"2015","year":"2015","journal-title":"J. Immunol. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.7150\/ijbs.7.1230","article-title":"A DNA vaccine encoding for TcSSP4 induces protection against acute and chronic infection in experimental Chagas disease","volume":"7","year":"2011","journal-title":"Int. J. Biol. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1111\/pim.12017","article-title":"Differential immune response in mice immunized with the A, R or C domain from TcSP protein of Trypanosoma cruzi or with the coding DNAs","volume":"35","year":"2013","journal-title":"Parasite Immunol."},{"key":"ref_34","first-page":"1","article-title":"Plasmid DNA immunization with Trypanosoma cruzi genes induces cardiac and clinical protection against Chagas disease in the canine model","volume":"43","author":"Reyes","year":"2012","journal-title":"Vet. Res."},{"key":"ref_35","first-page":"1","article-title":"Specific humoral and cellular immunity induced by Trypanosoma cruzi DNA immunization in a canine model","volume":"44","author":"Zenteno","year":"2013","journal-title":"Vet. Res."},{"key":"ref_36","first-page":"826570","article-title":"Effect of the plasmid-DNA vaccination on macroscopic and microscopic damage caused by the experimental chronic Trypanosoma cruzi infection in the canine model","volume":"2013","author":"Vallejo","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"ref_37","unstructured":"(2019, December 16). Norma Oficial Mexicana NOM-062-ZOO-1999: Especificaciones para el Cuidado y uso de Animales de Laboratorio, Available online: https:\/\/www.gob.mx\/cms\/uploads\/attachment\/file\/203498\/NOM-062-ZOO-1999_220801.pdf."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1089\/vbz.2010.0231","article-title":"Trypanosoma cruzi connatal transmission in dogs with Chagas disease: Experimental case report","volume":"11","author":"Reyes","year":"2011","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1006\/expr.1996.0074","article-title":"American trypanosomiosis: In situ and generalized features of parasitism and inflammation kinetics in a murine model","volume":"83","author":"Reyes","year":"1996","journal-title":"Exp. Parasitol."},{"key":"ref_40","unstructured":"(2002). Control of Chagas Disease. Second Report of the WHO expert Committee, World Health Organization. Available online: http:\/\/apps.who.int\/iris\/bitstream\/10665\/42443\/1\/WHO_TRS_905.pdf."},{"key":"ref_41","unstructured":"Boon, J.A. (2011). Veterinary Echocardiography, Wiley-Blackwell."},{"key":"ref_42","unstructured":"(2019, December 16). Norma Oficial Mexicana NOM-033-ZOO-1995, Sacrificio Humanitario de los Animales dom\u00e9Sticos y Silvestres, Available online: http:\/\/www.dof.gob.mx\/nota_detalle.php?codigo=5376424&fecha=18\/12\/2014."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"52","DOI":"10.4103\/2211-4122.203557","article-title":"Right ventricular outflow tract systolic excursion and fractional shortening: Can these echocardiographic parameters be used for the assessment of right ventricular function?","volume":"27","author":"Allam","year":"2017","journal-title":"J. Cardiovasc. Echogr."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1111\/echo.13438","article-title":"Echocardiographic linear fractional shortening for quantification of right ventricular systolic function\u2014A cardiac magnetic resonance validation study","volume":"34","author":"Srinivasan","year":"2017","journal-title":"Echocardiography"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1067\/mje.2001.111155","article-title":"Tissue Doppler imaging enables the identification of diastolic dysfunction of pseudonormal pattern in Chagas\u2019 disease","volume":"14","author":"Barros","year":"2001","journal-title":"J. Am. Soc. Echocardiogr."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2460\/ajvr.75.1.59","article-title":"Feasibility and reproducibility of echocardiographic assessment of regional left atrial deformation and synchrony by tissue Doppler ultrasonographic imaging in healthy dogs","volume":"75","author":"Guglielmini","year":"2014","journal-title":"Am. J. Vet. Res."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Bening, C., Hamouda, K., and Leyh, R. (2016). Sex differences in volume overload in skinned fibers. BMC Cardiovasc. Disord., 16.","DOI":"10.1186\/s12872-016-0370-8"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.jacc.2005.08.022","article-title":"ACC\/AHA 2005 guideline update for the diagnosis and management of chronic heart failure in the adult: A report of the American College of Cardiology\/American Heart Association Task Force on Practice Guidelines (Writing Committee to Update the 2001 Guidelines for the Evaluation and Management of Heart Failure)","volume":"46","author":"Hunt","year":"2005","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e012443","DOI":"10.1161\/JAHA.119.012443","article-title":"Natural History of Cardiomyopathy in Adult Dogs with Golden Retriever Muscular Dystrophy","volume":"8","author":"Guo","year":"2019","journal-title":"J. Am. Heart Assoc."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1111\/j.1939-1676.2006.tb02829.x","article-title":"Prognostic indicators for dogs with dilated cardiomyopathy","volume":"20","author":"Borgarelli","year":"2006","journal-title":"J. Vet. Intern. Med."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Piantedosi, D., Piscitelli, A., De Rosa, A., Serrano Lopez, B., Claretti, M., Boz, E., Mazzoni, L., Navalon Calvo, I., Ciaramella, P., and Bussadori, C. (2019). Evaluation of left ventricular dimension and systolic function by standard transthoracic echocardiography before and 24-h after percutaneous closure of patent ductus arteriosus in 120 dogs. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0223676"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1016\/j.cardfail.2011.08.007","article-title":"Myocardial deformation analysis in Chagas heart disease with the use of speckle tracking echocardiography","volume":"17","author":"Sitges","year":"2011","journal-title":"J. Card. 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