{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T03:12:14Z","timestamp":1788318734927,"version":"build-2803163510"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T00:00:00Z","timestamp":1586476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The National Research Institute of Animal Production, Poland","award":["No 3418.1."],"award-info":[{"award-number":["No 3418.1."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The laparoscopic method of recovering oocytes in goats and sheep is one of the minimally invasive methods used in the biotechnology of animal reproduction. It allows for good quality oocytes that are suitable for in vitro maturation and fertilization to be recovered. The limitation of using the laparoscopic ovum pick-up (L-OPU) method in goat and sheep is its changing effectiveness and the lack of repeatability of results, as well as the varying effectiveness of different variants of the method. Therefore, it is necessary to develop effective non-invasive techniques allowing for multiple good quality oocyte recovery that would be suitable for in vitro maturation and fertilization. In this study, four different L-OPU variants were described in goats and sheep. Various techniques of recovering oocytes were discussed, including the techniques of conducting the operation, various tools for recovering oocytes, and different plans of hormonal stimulation. Recovery rates were 35% (Variant I), 57% (Variant II), 72% (Variant III), and 67% (Variant IV). After evaluation, 94% (both Variant I and II), 93% (Variant III), and 84% (Variant IV) of the oocytes were qualified for in vitro maturation. The results of the study show that the proposed technique of laparoscopic recovery of oocytes allows a sufficient number of ovarian cells suitable for in vitro culture to be obtained and as a consequence it makes them useful in in vitro maturation\/in vitro fertilization (IVM\/IVF) programs or cloning. The method allows for a fast and effective conduct of the operation in a living donor with minimal invasiveness while preserving the excellent condition of animals.<\/jats:p>","DOI":"10.3390\/ani10040658","type":"journal-article","created":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T10:41:52Z","timestamp":1586774512000},"page":"658","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["L-OPU in Goat and Sheep\u2014Different Variants of the Oocyte Recovery Method"],"prefix":"10.3390","volume":"10","author":[{"given":"Jaros\u0142aw","family":"Wieczorek","sequence":"first","affiliation":[{"name":"University Centre of Veterinary Medicine UJ-UR, University of Agriculture in Krakow, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jurij","family":"Koseniuk","sequence":"additional","affiliation":[{"name":"Artvimed Centre for Reproductive Medicine, 30-147 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0068-6407","authenticated-orcid":false,"given":"Maria","family":"Skrzyszowska","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, 30-083 Balice\/Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miros\u0142aw","family":"Ceg\u0142a","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, 30-083 Balice\/Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1016\/S0093-691X(02)01128-7","article-title":"Production of transgenic goats by pronuclear microinjection of in vitro produced zygotes derived from oocytes recovered by laparoscopy","volume":"59","author":"Baldassarre","year":"2003","journal-title":"Theriogenology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.theriogenology.2017.10.042","article-title":"Laparoscopic ovarian biopsy pick-up method for goats","volume":"107","author":"Alves","year":"2018","journal-title":"Theriogenology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.5713\/ajas.19.0486","article-title":"Research advances in reproduction for dairy goats","volume":"32","author":"Luo","year":"2019","journal-title":"Asian-Australas J. Anim. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"273","DOI":"10.21451\/1984-3143-AR871","article-title":"Advances and limitations of in vitro embryo production in sheep and goats","volume":"13","author":"Menchaca","year":"2016","journal-title":"Anim. Reprod."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.theriogenology.2016.04.025","article-title":"Nonsurgical embryo recovery and transfer in sheep and goats","volume":"86","author":"Fonseca","year":"2016","journal-title":"Theriogenology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1016\/S0093-691X(01)00486-1","article-title":"Embryo production by ovum pick up from live donors","volume":"55","author":"Galli","year":"2000","journal-title":"Theriogenology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0093-691X(98)00235-0","article-title":"State of the art in sheep\u2014Goat embryo transfer","volume":"51","author":"Cogine","year":"1999","journal-title":"Theriogenology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1071\/RD09222","article-title":"New approaches to superovulation and embryo transfer in small ruminants","volume":"22","author":"Menchaca","year":"2010","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0093-691X(98)90598-2","article-title":"Laparoscopic techniques for the recovery and transfer of microinjected goat zygotes","volume":"49","author":"Prokofiev","year":"1998","journal-title":"Theriogenology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1071\/RD04033","article-title":"Multiple factors affecting the efficiency of multiple ovulation and embryo transfer in sheep and goats","volume":"16","author":"Baird","year":"2004","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.theriogenology.2016.04.027","article-title":"Recent advances in in vitro embryo production in small ruminants","volume":"86","author":"Paramio","year":"2016","journal-title":"Theriogenology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1016\/j.theriogenology.2014.02.001","article-title":"In vitro production of small ruminant embryos: Late improvements and further research","volume":"81","author":"Panneau","year":"2014","journal-title":"Theriogenology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"803","DOI":"10.24425\/pjvs.2018.125603","article-title":"The repeatable method of laparoscopic ovum pick-up (OPU) in sheep: Clinical aspects and efficiency of the method","volume":"21","author":"Wieczorek","year":"2018","journal-title":"Pol. J. Vet. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/S0093-691X(99)00164-8","article-title":"Repeated endoscopic ovum pick-up in sheep","volume":"52","author":"Stangl","year":"1999","journal-title":"Theriogenology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/S0093-691X(99)80014-4","article-title":"Transvaginal ultrasound-guided oocyte retriveal following FSH stimulation of domestic goats","volume":"51","author":"Graff","year":"1998","journal-title":"Theriogenology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S0093-691X(02)01270-0","article-title":"Current status of embryo technologies in sheep and goat","volume":"59","author":"Baril","year":"2003","journal-title":"Theriogenology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/j.1439-0531.2006.00735.x","article-title":"In vitro fertilization and development of OPU derived goat and sheep oocytes","volume":"42","author":"Cox","year":"2007","journal-title":"Reprod. Domest. Anim."},{"key":"ref_18","first-page":"85","article-title":"Factors affecting the oocyte recovery rate from ovarian follicles of gilts","volume":"5","author":"Wieczorek","year":"2005","journal-title":"Ann. Anim. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0378-4320(94)90014-0","article-title":"Technique for efficient recovery of sheep oocytes by laparoscopic folliculocentesis","volume":"359","author":"Baldassarre","year":"1994","journal-title":"Anim. Reprod. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1111\/j.1439-0531.2006.00648.x","article-title":"Ovum pick-up in sheep: A comparison between different aspiration devices for optimal oocyte retrieval","volume":"41","author":"Rodriguez","year":"2006","journal-title":"Reprod. Domest. Anim."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1002\/mrd.10199","article-title":"Transgenic goats produced by DNA pronuclear microinjection of in vitro derived zygotes","volume":"63","author":"Wang","year":"2002","journal-title":"Mol. Reprod. Dev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.5713\/ajas.2013.13786","article-title":"Follicle Stimulating Hormone (FSH) Dosage Based on Body Weight Enhances Ovulatory Responses and Subsequent Embryo Production in Goats","volume":"27","author":"Rahman","year":"2014","journal-title":"Asian-Australas J. Anim. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1530\/jrf.0.0670395","article-title":"Endocrine responses of goats after induction of superovulation with PMSG and FSH","volume":"67","author":"Armstrong","year":"1983","journal-title":"J. Reprod. Fertil."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/0921-4488(92)90042-3","article-title":"Follicle stimulating hormone versus pregnant mare serum gonadotropin for superovulation of dairy goats","volume":"8","author":"Pendleton","year":"1992","journal-title":"Small Rumin. Res."},{"key":"ref_25","first-page":"819","article-title":"Comparison of Superovulatory Effect of Equine Chorionic Gonadotrophin and Follicle Stimulating Hormone on Embryo Production in Crossbred (Boer \u00d7 Katjang) Goats","volume":"46","author":"Rahman","year":"2014","journal-title":"Pak. J. Zool."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/S0093-691X(97)00271-9","article-title":"Repeated endoscopic ovum pick-up in hormonally untreated ewes: A new technique","volume":"48","author":"Treuer","year":"1997","journal-title":"Theriogenology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1590\/S0102-09352014000100020","article-title":"Reproductive efficiency of adult and prepubertal goats subjected to repeated follicular aspiration","volume":"66","author":"Cordeiro","year":"2014","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.anireprosci.2011.08.001","article-title":"Laparoscopic ovum collection in sheep: Gross and microscopic evaluation of the ovary and influence on oocyte production","volume":"127","author":"Teixeira","year":"2011","journal-title":"Anim. Rep. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/0378-4320(84)90048-4","article-title":"Number and quality of oocytes in relation to age of cattle","volume":"7","author":"Katska","year":"1984","journal-title":"Anim. Reprod. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1016\/j.theriogenology.2006.05.005","article-title":"Laparoscopic ovum pick-up and in vitro production of sika deer embryos: Effect of season and culture conditions","volume":"66","author":"Locatelli","year":"2006","journal-title":"Theriogenology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/S0093-691X(03)00090-6","article-title":"Developmental competence of prepubertal and adult goat oocytes cultured in semi-defined media following laparoscopic recovery","volume":"60","author":"Koeman","year":"2003","journal-title":"Theriogenology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/0093-691X(88)90310-X","article-title":"Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries","volume":"30","author":"Pieterse","year":"1988","journal-title":"Theriogenology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1262\/jrd.51.109","article-title":"Ovarian activity and oocyte development during follicular development in pigs at different reproductive phases estimated by the repeated endoscopic method","volume":"51","author":"Torner","year":"2005","journal-title":"J. Reprod. Dev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1262\/jrd.17016","article-title":"Comparison of follicular and oocyte development and reproductive hormone secretion during the ovulatory period in Hungarian native breed, Mangalica, and Landrace gilts","volume":"51","author":"Egerszegi","year":"2005","journal-title":"J. Reprod. Dev."},{"key":"ref_35","first-page":"153","article-title":"Transwaginalna metoda uzyskiwania oocyt\u00f3w bydl\u0119cych: Efektywno\u015b\u0107 aspiracji, mo\u017cliwo\u015bci rozwojowe oocyt\u00f3w oraz zastosowanie w hodowli","volume":"2","author":"Gogol","year":"1998","journal-title":"Biotechnologia"},{"key":"ref_36","first-page":"89","article-title":"Oocyte recovery rate with application of laparoscopic ovum pick-up in pigs","volume":"7","author":"Wieczorek","year":"2007","journal-title":"Ann. Anim. Sci."},{"key":"ref_37","first-page":"189","article-title":"Ovum pick up in swine: The influence of aspiration vacuum pressure on oocyte recovery from preovulatory follicles","volume":"45","author":"Torner","year":"1997","journal-title":"Acta. Vet. Hung."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0093-691X(01)00671-9","article-title":"Advances in the production and propagation of transgenic goats using laparoscopic ovum pick-up and in vitro embryo production technologies","volume":"57","author":"Baldassarre","year":"2002","journal-title":"Theriogenology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1071\/RD07024","article-title":"Laparoscopic ovum pick-up followed by in vitro embryo production for the reproductive rescue of aged goats of high genetic value","volume":"19","author":"Baldassarre","year":"2007","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.repbio.2013.09.005","article-title":"Assessment of the reproductive parameters, laparoscopic oocyte recovery and the first embryos produced in vitro from endangered Caninde goats (Capra hircus)","volume":"13","author":"Pereira","year":"2013","journal-title":"Reprod. Biol."},{"key":"ref_41","first-page":"55","article-title":"Effect of repeated laparoscopic ovum pick-up on yield and quality of oocytes in goats","volume":"18","author":"Rahman","year":"2015","journal-title":"Malays. J. Anim. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1631\/jzus.2007.B0465","article-title":"Qualitative and quantitative analysis of goat ovaries, follicles and oocytes in view of in vitro production of embryos","volume":"8","author":"Islam","year":"2007","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1111\/j.1439-0531.2005.00603.x","article-title":"Repeat ovum pick-up and in vitro embryo production from adult ewes with and without FSH treatment","volume":"40","author":"Morton","year":"2005","journal-title":"Reprod. Domest. Anim."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/4\/658\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:17:25Z","timestamp":1760174245000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/4\/658"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,10]]},"references-count":43,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["ani10040658"],"URL":"https:\/\/doi.org\/10.3390\/ani10040658","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,10]]}}}