{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T11:22:49Z","timestamp":1792236169894,"version":"4.4.0"},"reference-count":84,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,12]],"date-time":"2020-04-12T00:00:00Z","timestamp":1586649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The poultry industry needs efficient antibiotic alternatives to prevent necrotic enteritis (NE) infections. Here, we evaluate the effects of probiotic and\/or prebiotic dietary supplementation on performance, meat quality and carcass traits, using only an NE coinfection model, in broiler chickens. Three hundred and twenty-four healthy Ross 308 broiler chicks are allocated into six groups. Taking a 35 d feeding trial, the chicks are fed a basal diet with 0.0, 0.1, 0.5, 0.12, 0.5 + 0.12, and 0.2 g Kg\u22121 for the control (T1), Avilamycin (Maxus; T2), live probiotic (CloStat (Bacillus subtilis);T3), natural phytobiotic compounds (Sangrovit Extra (sanguinarine and protopine); T4), CloStat + Sangrovit Extra (T5), and spore probiotic strain (Gallipro Tect (Bacillus subtilis spores); T6) treatments, respectively. Occurring at 15 days-old, chicks are inoculated with Clostridium perfringens. The obtained results reveal that all feed additives improve the performance, feed efficiency, and survival rate, and reduces the intestinal lesions score compared with the control group. The T6 followed by T3 groups show a significant (p &lt; 0.05) increase in some carcass traits, such as dressing, spleen, and thymus percentages compared with other treatments. Also, T5 and T6 have significantly recorded the lowest temperature and pHu values and the highest hardness and chewiness texture values compared to the other treated groups. To conclude, probiotics combined with prebiotic supplementation improves the growth, meat quality, carcass characterization and survival rate of NE-infected broiler chickens by modulating gut health conditions and decreasing lesion scores. Moreover, it could be useful as an ameliorated NE disease alternative to antibiotics in C. perfringens coinfected poultry.<\/jats:p>","DOI":"10.3390\/ani10040669","type":"journal-article","created":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T10:41:52Z","timestamp":1586774512000},"page":"669","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Ameliorative Effects of Antibiotic-, Probiotic- and Phytobiotic-Supplemented Diets on the Performance, Intestinal Health, Carcass Traits, and Meat Quality of Clostridium perfringens-Infected Broilers"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8673-3026","authenticated-orcid":false,"given":"Elsayed O.S.","family":"Hussein","sequence":"first","affiliation":[{"name":"Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shamseldein H.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, College of Veterinary Medicine, Sudan University of Science and Technology, Khartoum 12942, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7998-0344","authenticated-orcid":false,"given":"Alaeldein M.","family":"Abudabos","sequence":"additional","affiliation":[{"name":"Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9865-1589","authenticated-orcid":false,"given":"Gamaleldin M.","family":"Suliman","sequence":"additional","affiliation":[{"name":"Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2831-8534","authenticated-orcid":false,"given":"Mohamed E.","family":"Abd El-Hack","sequence":"additional","affiliation":[{"name":"Poultry Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3247-5898","authenticated-orcid":false,"given":"Ayman A.","family":"Swelum","sequence":"additional","affiliation":[{"name":"Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia"},{"name":"Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah","family":"N. Alowaimer","sequence":"additional","affiliation":[{"name":"Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Abudabos, A.M., Ali, M.H., Nassan, M.A., and Saleh, A.A. (2019). Ameliorative Effect of Bacillus subtilis on Growth Performance and Intestinal Architecture in Broiler Infected with Salmonella. Animals, 9.","DOI":"10.3390\/ani9040190"},{"key":"ref_2","unstructured":"Niilo, L. (1993). Enterotoxemic Clostridium perfringens. Pathogenesis of Bacterial Infections in Animals, Wiley-Blackwell."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1128\/CMR.3.1.66","article-title":"Toxigenic clostridia","volume":"3","author":"Hatheway","year":"1990","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.vetmic.2008.06.001","article-title":"Immunization with recombinant alpha toxin partially protects broiler chicks against experimental challenge with Clostridium perfringens","volume":"133","author":"Cooper","year":"2009","journal-title":"Vet. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.tim.2008.09.005","article-title":"Rethinking our understanding of the pathogenesis of necrotic enteritis in chickens","volume":"17","author":"Rood","year":"2009","journal-title":"Trends Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1128\/JB.188.4.1585-1598.2006","article-title":"Complete sequencing and diversity analysis of the enterotoxin-encoding plasmids in Clostridium perfringens type A non-food-borne human gastrointestinal disease isolates","volume":"188","author":"Miyamoto","year":"2006","journal-title":"J. Bacteriol."},{"key":"ref_7","first-page":"251","article-title":"Toxinas de Clostridium perfringens","volume":"41","author":"Morris","year":"2009","journal-title":"Rev. Argent. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"931","DOI":"10.20506\/rst.31.3.2169","article-title":"Prevalence of Clostridium perfringens type A isolates in commercial broiler chickens and parent broiler breeder hens in Egypt","volume":"31","author":"Osman","year":"2012","journal-title":"Rev. Sci. Tech. Off. Int. Epiz"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"557","DOI":"10.3382\/japr.2013-00925","article-title":"Necrotic enteritis: Applications for the poultry industry","volume":"23","author":"Paiva","year":"2014","journal-title":"J. Appl. Poult. Res."},{"key":"ref_10","unstructured":"Fenichel, G.M. (2009). Clinical Pediatric Neurology: A Signs and Symptoms Approach, Elsevier Health Sciences."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1080\/03079457.2016.1165793","article-title":"Efficacy of avilamycin for the prevention of necrotic enteritis caused by a pathogenic strain of Clostridium perfringens in broiler chickens","volume":"45","author":"Paradis","year":"2016","journal-title":"Avian Pathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"210","DOI":"10.2307\/1593030","article-title":"Efficacy of narasin in the prevention of necrotic enteritis in broiler chickens","volume":"45","author":"Brennan","year":"2001","journal-title":"Avian Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1128\/CMR.16.2.175-188.2003","article-title":"Antimicrobial growth promoters used in animal feed: Effects of less well known antibiotics on gram-positive bacteria","volume":"16","author":"Butaye","year":"2003","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"128","DOI":"10.3382\/ps\/pey332","article-title":"Characterization of Clostridium perfringens recovered from broiler chicken affected by necrotic enteritis","volume":"98","author":"Mwangi","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0958-6946(99)00044-8","article-title":"Prebiotics, probiotics and human gut microbiology","volume":"9","author":"Fooks","year":"1999","journal-title":"Int. Dairy J."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Arif, M., Iram, A., Bhutta, M.A., Naiel, M.A., Abd El-Hack, M.E., Othman, S.I., Allam, A.A., Amer, M.S., and Taha, A.E. (2020). The Biodegradation Role of Saccharomyces cerevisiae against Harmful Effects of Mycotoxin Contaminated Diets on Broiler Performance, Immunity Status, and Carcass characteristics. Animals, 10.","DOI":"10.3390\/ani10020238"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"El-Shall, N.A., Awad, A.M., Abd El-Hack, M.E., Naiel, M.A., Othman, S.I., Allam, A.A., and Sedeik, M.E. (2020). The Simultaneous Administration of a Probiotic or Prebiotic with Live Salmonella Vaccine Improves Growth Performance and Reduces Fecal Shedding of the Bacterium in Salmonella-Challenged Broilers. Animals, 10.","DOI":"10.3390\/ani10010070"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.3389\/fmicb.2015.01336","article-title":"Alternatives to antibiotics to prevent necrotic enteritis in broiler chickens: A microbiologist\u2019s perspective","volume":"6","author":"Caly","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1093\/ps\/82.4.640","article-title":"Alternatives to antibiotics: Bacteriocins, antimicrobial peptides and bacteriophages","volume":"82","author":"Joerger","year":"2003","journal-title":"Poult. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"108","DOI":"10.4161\/gmic.26945","article-title":"Intestinal microbiome of poultry and its interaction with host and diet","volume":"5","author":"Pan","year":"2014","journal-title":"Gut Microbes"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4081","DOI":"10.4049\/jimmunol.1403169","article-title":"No vacancy: How beneficial microbes cooperate with immunity to provide colonization resistance to pathogens","volume":"194","author":"Raffatellu","year":"2015","journal-title":"J. Immunol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1128\/AEM.71.2.968-978.2005","article-title":"Screening for Bacillus isolates in the broiler gastrointestinal tract","volume":"71","author":"Barbosa","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","first-page":"115","article-title":"Characterization of cecal microbiota and response to an orally administered lactobacillus probiotic strain in the broiler chicken","volume":"14","author":"Salmon","year":"2008","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1637\/9106-101509-ResNote.1","article-title":"Bacillus licheniformis prevents necrotic enteritis in broiler chickens","volume":"54","author":"Knap","year":"2010","journal-title":"Avian Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0378-1135(03)00077-4","article-title":"Competitive exclusion by Bacillus subtilis spores of Salmonella enterica serotype Enteritidis and Clostridium perfringens in young chickens","volume":"94","author":"Woodward","year":"2003","journal-title":"Vet. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5392","DOI":"10.3382\/ps\/pez368","article-title":"Effect of Bacillus subtilis DSM 32315 on the intestinal structural integrity and growth performance of broiler chickens under necrotic enteritis challenge","volume":"98","author":"Sokale","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"370","DOI":"10.3382\/ps.2012-02528","article-title":"Bacillus subtilis PB6 improves intestinal health of broiler chickens challenged with Clostridium perfringens-induced necrotic enteritis","volume":"92","author":"Jayaraman","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"734264","DOI":"10.1016\/j.aquaculture.2019.734264","article-title":"Ameliorative effect of diets supplemented with rosemary (Rosmarinus officinalis) on aflatoxin B1 toxicity in terms of the performance, liver histopathology, immunity and antioxidant activity of Nile Tilapia (Oreochromis niloticus)","volume":"511","author":"Naiel","year":"2019","journal-title":"Aquaculture"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Alagawany, M., Abd El-Hack, M.E., Al-Sagheer, A.A., Naiel, M.A., Saadeldin, I.M., and Swelum, A.A. (2018). Dietary cold pressed watercress and coconut oil mixture enhances growth performance, intestinal microbiota, antioxidant status, and immunity of growing rabbits. Animals, 8.","DOI":"10.3390\/ani8110212"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Al-Sagheer, A.A., Abd El-Hack, M.E., Alagawany, M., Naiel, M.A., Mahgoub, S.A., Badr, M.M., Hussein, E.O., Alowaimer, A.N., and Swelum, A.A. (2019). Paulownia leaves as a new feed resource: Chemical composition and effects on growth, carcasses, digestibility, blood biochemistry, and intestinal bacterial populations of growing rabbits. Animals, 9.","DOI":"10.3390\/ani9030095"},{"key":"ref_31","first-page":"131","article-title":"Phytobiotics: Alternatives to antibiotic growth promoters in monogastric animal feeds","volume":"15","author":"Vidanarachchi","year":"2005","journal-title":"Recent Adv. Anim. Nutr. Aust."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1080\/09712119.2017.1383258","article-title":"The effect of phytogenics on growth traits, blood biochemical and intestinal histology in broiler chickens exposed to Clostridium perfringens challenge","volume":"46","author":"Abudabos","year":"2018","journal-title":"J. Appl. Anim. Res."},{"key":"ref_33","unstructured":"El-Sheikh, S.M., Khairy, M.H., Eleiwa, N.Z., Abdalla, O.E., and El-Monsef, A.G.A. (2018, January 16\u201319). Effect of sanguinarine phytobiotic, sodium butyrate compared to ampicillin on controlling necrotic enteritis in broiler chickens. Proceedings of the Veterinary Medicine In-between Health & Economy (VMHE), Cairo, Egypt."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.3382\/ps\/pex359","article-title":"The gastrointestinal microbiome and its association with the control of pathogens in broiler chicken production: A review","volume":"97","author":"Clavijo","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.2307\/1589625","article-title":"The prevention of experimentally induced necrotic enteritis in chickens by avoparcin","volume":"23","author":"Prescott","year":"1979","journal-title":"Avian Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.rvsc.2005.10.006","article-title":"Responses of broiler chickens orally challenged with Clostridium perfringens isolated from field cases of necrotic enteritis","volume":"81","author":"Olkowski","year":"2006","journal-title":"Res. Vet. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1590\/S0074-02761999000500022","article-title":"Antibacterial activity of Ocimum gratissimum L. essential oil","volume":"94","author":"Nakamura","year":"1999","journal-title":"Mem\u00f3rias Inst. Oswaldo Cruz"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1080\/00071667908416580","article-title":"The response of cage-reared broiler cockerels to dietary supplements of nitrovin, zinc bacitracin or penicillin used singly or in paired combinations","volume":"20","author":"Griffin","year":"1979","journal-title":"Br. Poult. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Hussein, E.O.S., Ahmed, S.H., Abudabos, A.M., Aljumaah, M.R., Alkhlulaifi, M.M., Nassan, M.A., Suliman, G.M., Naiel, M.A.E., and Swelum, A.A. (2020). Effect of Antibiotic, Phytobiotic and Probiotic Supplementation on Growth, Blood Indices and Intestine Health in Broiler Chicks Challenged with Clostridium perfringens. Animals, 10.","DOI":"10.3390\/ani10030507"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1093\/japr\/12.1.60","article-title":"Using competitive exclusion, mannan-oligosaccharide and other intestinal products to control necrotic enteritis","volume":"12","author":"Hofacre","year":"2003","journal-title":"J. Appl. Poult. Res."},{"key":"ref_41","first-page":"467","article-title":"Necrotic enteritis in broiler chickens II. Pathology and proposed pathogenesis","volume":"38","author":"Long","year":"1974","journal-title":"Can. J. Comp. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"579","DOI":"10.2307\/1592685","article-title":"Use of Aviguard and other intestinal bioproducts in experimental Clostridium perfringens-associated necrotizing enteritis in broiler chickens","volume":"42","author":"Hofacre","year":"1998","journal-title":"Avian Dis."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"435","DOI":"10.3382\/ps.2012-02360","article-title":"Effects of outdoor access on growth performance, carcass composition, and meat characteristics of broiler chickens","volume":"92","author":"Chen","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S0309-1740(01)00124-3","article-title":"Effect of organic production system on broiler carcass and meat quality","volume":"60","author":"Castellini","year":"2002","journal-title":"Meat Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1111\/j.1365-2621.1978.tb15263.x","article-title":"Relationship of myofibril fragmentation index to certain chemical, physical and sensory characteristics of bovine longissimus muscle","volume":"43","author":"Culler","year":"1978","journal-title":"J. Food Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/S0065-2628(08)60141-X","article-title":"Biochemistry of meat hydration","volume":"Volume 10","author":"Hamm","year":"1961","journal-title":"Advances in Food and Nutrition Research"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1016\/j.foodchem.2009.08.034","article-title":"Protease activity and the ultrastructure of broiler chicken PSE (pale, soft, exudative) meat","volume":"119","author":"Wilhelm","year":"2010","journal-title":"Food Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"196","DOI":"10.2527\/2005.831196x","article-title":"Characterization of biological types of cattle (Cycle VII): Carcass, yield, and longissimus palatability traits","volume":"83","author":"Wheeler","year":"2005","journal-title":"J. Anim. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2307\/3001478","article-title":"Multiple range and multiple F tests","volume":"11","author":"Duncan","year":"1955","journal-title":"Biometrics"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.3389\/fimmu.2017.01592","article-title":"Probiotic enhanced intestinal immunity in broilers against subclinical necrotic enteritis","volume":"8","author":"Wang","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_51","first-page":"267","article-title":"Effect of probiotics on feed consumption, live weight gain and production cost in broilers","volume":"84","author":"Kefali","year":"2007","journal-title":"Indian Vet. J."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"De Jesus, R., Waitzberg, D., and Campos, F. (2000). More Channels Showcase Channel Catalog. Rev. Assoc. Med. Bras., 46.","DOI":"10.1590\/S0104-42302000000300010"},{"key":"ref_53","first-page":"95","article-title":"Effect of dietary yeast supplementation on broiler performance","volume":"32","year":"2012","journal-title":"Egyp. Poult. Sci. J."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.rvsc.2011.05.021","article-title":"Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology","volume":"93","author":"Sen","year":"2012","journal-title":"Res. Vet. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"155","DOI":"10.5958\/2277-940X.2015.00026.1","article-title":"Effects of probiotics supplementation on growth performance, feed conversion ratio and economics of broilers","volume":"5","author":"Patel","year":"2015","journal-title":"J. Anim. Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1111\/wrr.12607","article-title":"Probiotics or pro-healers: The role of beneficial bacteria in tissue repair","volume":"25","author":"Lukic","year":"2017","journal-title":"Wound Repair Regen."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"296","DOI":"10.2141\/jpsa.43.296","article-title":"Effect of probiotic on performance and immunocompetence in broiler chicks","volume":"43","author":"Khaksefidi","year":"2006","journal-title":"J. Poult. Sci."},{"key":"ref_58","first-page":"25","article-title":"Effect of dietary supplementation of multi-strain probiotic on broiler growth performance","volume":"25","author":"Anjum","year":"2005","journal-title":"Pak. Vet. J."},{"key":"ref_59","first-page":"85","article-title":"Effects of dietary supplementation of probiotics on broiler chicken","volume":"9","author":"Singh","year":"2009","journal-title":"Anim. Nutr. Feed Technol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1097\/00054725-200105000-00010","article-title":"Adaptation of bacteria to the intestinal niche: Probiotics and gut disorder","volume":"7","author":"Dunne","year":"2001","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_61","unstructured":"Windisch, W., and Kroismayr, A. (2016). The effects of phytobiotics on performance and gut function in monogastrics. World Nutrition Forum: The Future of Animal Nutrition, Engormix."},{"key":"ref_62","unstructured":"Gauthier, R. (2003, January 7\u20132003;10). Poultry therapeutics: New alternatives in nuevas alternativas en therapeutica aviar. Proceedings of the XVIII Latin American Poultry Congress, Santa Cruz, Bolivia."},{"key":"ref_63","first-page":"672","article-title":"Effects of probiotic, prebiotic, and synbiotic with and without feed restriction on performance, hematological indices and carcass characteristics of broiler chickens","volume":"30","author":"Saleh","year":"2017","journal-title":"Asian Australas. J. Anim. Sci."},{"key":"ref_64","first-page":"45","article-title":"Influence of probiotic, prebiotic and\/or yeast supplementation in broiler diets on the productivity, immune response and slaughter traits","volume":"1","author":"Riad","year":"2010","journal-title":"J. Anim. Poult. Prod."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1080\/00071660601050755","article-title":"Holo-analysis of the efficacy of Bio-Mos\u00ae in broiler nutrition","volume":"48","author":"Rosen","year":"2007","journal-title":"Br. Poult. Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1093\/japr\/16.2.280","article-title":"Effects of mannanoligosaccharide on growth performance, the development of gut microflora, and gut function of broiler chickens raised on new litter","volume":"16","author":"Yang","year":"2007","journal-title":"J. Appl. Poult. Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1093\/japr\/2.1.75","article-title":"Practical use of feed enzymes for turkeys and broilers","volume":"2","author":"Ferket","year":"1993","journal-title":"J. Appl. Poult. Res."},{"key":"ref_68","first-page":"32","article-title":"Effect of dietary supplementation of various levels of Fermacto on the performance of broiler chicks","volume":"2","author":"Salamkhan","year":"2000","journal-title":"Int. J. Agric. Biol."},{"key":"ref_69","first-page":"37","article-title":"The influence of supplementation of lactobacillus sporogenes on the performance of broilers","volume":"22","author":"Panda","year":"2005","journal-title":"Indian J. Anim. Nutr."},{"key":"ref_70","first-page":"252","article-title":"Growth performance and carcass characteristics of broiler chicken fed with probiotics","volume":"36","author":"Banday","year":"2001","journal-title":"Indian J. Poult. Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"361","DOI":"10.3923\/ijps.2004.361.364","article-title":"The dynamics of probiotics on growth performance and immune response in broilers","volume":"3","author":"Kabir","year":"2004","journal-title":"Int. J. Poult. Sci."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"313","DOI":"10.14202\/vetworld.2016.313-319","article-title":"Effect of dietary supplementation of prebiotic, probiotic, and synbiotic on growth performance and carcass characteristics of broiler chickens","volume":"9","author":"Sarangi","year":"2016","journal-title":"Vet. World"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1093\/ps\/77.9.1259","article-title":"Growth performance, intestinal microbial populations, and serum cholesterol of broilers fed diets containing Lactobacillus cultures","volume":"77","author":"Jin","year":"1998","journal-title":"Poult. Sci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1093\/ps\/85.5.902","article-title":"Meat quality evaluation of minimally aged broiler breast fillets from five commercial genetic strains","volume":"85","author":"Mehaffey","year":"2006","journal-title":"Poult. Sci."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1093\/ps\/79.5.784","article-title":"The relationship of raw broiler breast meat color and pH to cooked meat color and pH","volume":"79","author":"Fletcher","year":"2000","journal-title":"Poult. Sci."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"3189","DOI":"10.4081\/ijas.2014.3189","article-title":"Effect of different probiotics on breast quality characteristics of broilers under Salmonella challenge","volume":"13","author":"Suliman","year":"2014","journal-title":"Italian J. Anim. Sci."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.3382\/ps.2007-00454","article-title":"The effects of low-atmosphere stunning and deboning time on broiler breast meat quality","volume":"87","author":"Battula","year":"2008","journal-title":"Poult. Sci."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"432","DOI":"10.3382\/ps.2008-00406","article-title":"The effects of feeding distillers dried grains with solubles on broiler meat quality","volume":"88","author":"Corzo","year":"2009","journal-title":"Poult. Sci."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1590\/S1516-635X2005000300006","article-title":"Carcass and cut yields and meat qualitative traits of broilers fed diets containing probiotics and prebiotics","volume":"7","author":"Pelicano","year":"2005","journal-title":"Braz. J. Poult. Sci."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0309-1740(00)00012-7","article-title":"A research note on factors affecting the determination of myofibrillar fragmentation","volume":"56","author":"Hopkins","year":"2000","journal-title":"Meat Sci."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1111\/j.1365-2621.1976.tb14384.x","article-title":"Myofibril fragmentation and shear resistance of three bovine muscles during postmortem storage","volume":"41","author":"Olson","year":"1976","journal-title":"J. Food Sci."},{"key":"ref_82","first-page":"379","article-title":"Effect of probiotics and thyme essential oil on the texture of cooked chicken breast meat","volume":"12","author":"Angelovicova","year":"2013","journal-title":"Acta Sci. Pol. Technol. Aliment."},{"key":"ref_83","first-page":"291","article-title":"Prebiotic properties of pea alpha-galactoside preparation applied to chicken per os","volume":"51","author":"Lisowski","year":"2003","journal-title":"Bull. Polish Acad. Sci. Biol. Sci."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1590\/S1516-635X2004000200005","article-title":"Probiotic and prebiotic utilization in diets for free-range broiler chickens","volume":"6","author":"Mendes","year":"2004","journal-title":"Braz. J. Poult. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/4\/669\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:08:52Z","timestamp":1760364532000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/4\/669"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,12]]},"references-count":84,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["ani10040669"],"URL":"https:\/\/doi.org\/10.3390\/ani10040669","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,12]]}}}