{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T14:54:17Z","timestamp":1790952857216,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,17]],"date-time":"2020-04-17T00:00:00Z","timestamp":1587081600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The experiment aimed to investigate the effects of dietary \u03b2-mannanase supplementation on growth performance, apparent total tract digestibility (ATTD) of nutrients, intestinal integrity, and the immunological and oxidative stress parameters in weaning pigs. A total of 64 newly weaning pigs (initial body weight: 6.96 \u00b1 0.70 kg) were allotted to two dietary treatments in eight replicates per treatment with four pigs per pen based on body weight and sex. Dietary treatments were 1.) CON (control: corn-soybean meal based basal diet) and 2.) \u03b2-mannanase (basal diet +0.06% \u03b2-mannanase). The \u03b2-mannanase supplementation did not affect growth performance, concentrations of acute phase protein, superoxide dismutase and glutathione peroxidase. However, the pigs fed the \u03b2-mannanase-supplemented diet had greater ATTD of ether extract, jejunum villus height, and villus height-to-crypt depth ratio, and lower crypt depth compared with those fed the CON diet (p &lt; 0.05). The pigs fed the \u03b2-mannanase-supplemented diet tended to have the lower count of E. coli in cecum than those fed the CON diet (p = 0.08). In conclusion, dietary \u03b2-mannanase supplementation did not affect growth performance, immune response and oxidative stress of weaning pigs, whereas it increased fat digestibility and had positive effects on intestinal integrity and cecum microflora by reducing the count of E.coli.<\/jats:p>","DOI":"10.3390\/ani10040703","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T05:48:52Z","timestamp":1587448132000},"page":"703","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Effects of Dietary \u03b2-Mannanase Supplementation on Growth Performance, Apparent Total Tract Digestibility, Intestinal Integrity, and Immune Responses in Weaning Pigs"],"prefix":"10.3390","volume":"10","author":[{"given":"Jae-Cheol","family":"Jang","sequence":"first","affiliation":[{"name":"Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8403-1231","authenticated-orcid":false,"given":"Young","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea"},{"name":"Department of Animal and Food Science, University of Wisconsin-River Falls, River Falls, WI 54022, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,17]]},"reference":[{"key":"ref_1","first-page":"485","article-title":"Non-starch polysaccharide-degrading enzymes increase the performance of broiler chickens fed wheat of low apparent metabolizable energy","volume":"125","author":"Choct","year":"1995","journal-title":"J. Nutr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/0076-6879(88)60174-1","article-title":"\u03b2-D-mannanase","volume":"160","author":"McCleary","year":"1988","journal-title":"Methods Enzymol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1093\/ps\/85.8.1430","article-title":"Levels of \u00df-mannan in soybean meal","volume":"85","author":"Hsiao","year":"2006","journal-title":"Poult. Sci."},{"key":"ref_4","first-page":"241","article-title":"Biotechnology aids to improve feed and feed digestion: Enzyme and fermentation","volume":"3","author":"Dierick","year":"1989","journal-title":"Arch. Anim. Nutr."},{"key":"ref_5","unstructured":"Lewis, A.J., and Southern, L.L. (2001). Feeding neonatal pigs. Swine Nutrition, CRC Press. [2nd ed.]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"843","DOI":"10.2527\/2006.844843x","article-title":"Effects of guar gum and cellulose on digesta passage rate, ileal microbial populations, energy and protein digestibility, and performance of grower pigs","volume":"84","author":"Patience","year":"2006","journal-title":"J. Anim. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1297-9716-43-4","article-title":"Effect of Saccharomyces cerevisiae var. Boulardii and \u03b2-galactomannan oligosaccharide on porcine intestinal epithelial and dendritic cells challenged in vitro with Escherichia coli F4 (K88)","volume":"43","author":"Badia","year":"2012","journal-title":"Vet. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1080\/07388550701775919","article-title":"Microbial mannanases: An overview of production and applications","volume":"27","author":"Dhawan","year":"2007","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.2527\/2002.8041012x","article-title":"Effects of \u03b2-mannanase addition to corn-soybean meal diets on growth performance, carcass traits, and nutrient digestibility of weanling and growing-finishing pigs","volume":"80","author":"Pettey","year":"2002","journal-title":"J. Anim. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"393","DOI":"10.15232\/pas.2014-01326","article-title":"Effects of endo-1,4-\u03b2-d-mannanase enzyme (Hemicell HT 1.5\u00d7) on the growth performance of nursery pigs","volume":"30","author":"Carr","year":"2014","journal-title":"Prof. Anim. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/s40104-018-0264-y","article-title":"Lipopolysaccharide immune stimulation but not \u03b2-mannanase supplementation affects maintenance energy requirements in young weaned pigs","volume":"9","author":"Huntley","year":"2018","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_12","unstructured":"National Research Council (NRC) (2012). Nutrient Requirements of Swine, NRC. [11th ed.]."},{"key":"ref_13","unstructured":"Varley, M.A., and Wiseman, J. (2001). Non-starch polysaccharides in the diets of young weaned piglets. The Weaner Pig: Nutrition and Management, CABI Publishing."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"727","DOI":"10.2527\/jas.2016.0752","article-title":"Disappearance of nutrients and energy in the stomach and small intestine, cecum, and colon of pigs fed corn-soybean meal diets containing distillers dried grains with solubles, wheat middlings, or soybean hulls","volume":"95","author":"Jaworski","year":"2017","journal-title":"J. Anim. Sci."},{"key":"ref_15","unstructured":"National Research Council (NRC) (1998). Nutrient Requirements of Swine, NRC. [10th ed.]."},{"key":"ref_16","unstructured":"Lewis, A.J., and Southern, L.L. (2001). Digestion and balance techniques in pigs. Swine Nutrition, CRC Press. [2nd ed.]."},{"key":"ref_17","unstructured":"AOAC International (2005). Official Methods of Analysis, AOAC International. [18th ed.]."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2496","DOI":"10.2527\/2003.81102496x","article-title":"Use of carbohydrases in corn soybean meal based nursery diets","volume":"81","author":"Kim","year":"2003","journal-title":"J. Anim. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.2527\/2004.8241053x","article-title":"Dietary supplementation with multienzyme preparations improves nutrient utilization and growth performance in weaned pigs","volume":"82","author":"Omogbenigun","year":"2004","journal-title":"J. Anim. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0377-8401(01)00194-8","article-title":"The role of dietary fibre in the digestive physiology of the pig","volume":"90","author":"Wenk","year":"2001","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/0076-6879(88)60162-5","article-title":"Enzymatic preparation of \u03b2-1,4-mannooligosaccharides and \u03b2-1,4-glucomannooligosaccharides","volume":"160","author":"Kusakabe","year":"1988","journal-title":"Methods Enzymol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.5713\/ajas.15.0275","article-title":"Effects of Supplemental Beta-mannanase on Digestible Energy and Metabolizable Energy Contents of Copra Expellers and Palm Kernel Expellers Fed to Pigs","volume":"28","author":"Kwon","year":"2015","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1093\/ajcn\/49.6.1192","article-title":"Wheat bran and wheat germ: Effect on digestion and intestinal absorption of dietary lipids in the rat","volume":"49","author":"Borel","year":"1989","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_24","first-page":"1","article-title":"Effect of dietary fibre on protein and fat digestibility and its consequences on diet formulation for growing and fattening pigs: A review","volume":"57","author":"Halas","year":"2007","journal-title":"Acta Agric. Scand Sect."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1042\/bj3140269","article-title":"Emulsification and lipolysis of triacylglycerols are altered by viscous soluble dietary fibres in acidic gastric medium in vitro","volume":"314","author":"Pasquier","year":"1996","journal-title":"Biochem. J."},{"key":"ref_26","first-page":"158","article-title":"Polysaccharides having a \u03b2-D-xylan backbone","volume":"44","author":"McCleary","year":"1986","journal-title":"Adv. Carbohydr. Chem. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1152\/physrev.00018.2001","article-title":"Free radicals in the physiological control of cell function","volume":"82","year":"2002","journal-title":"Physiol. Rev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1089\/ars.2006.8.1791","article-title":"Redox-sensitive kinases of the nuclear factor-kappaB signaling pathway","volume":"8","author":"Pantano","year":"2006","journal-title":"Antioxid. Redox Signal."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.anifeedsci.2018.07.002","article-title":"Supplemental effect of xylanase and mannanase on nutrient digestibility and gut health of nursery pigs studied using both in vivo and in vitro models","volume":"245","author":"Tiwari","year":"2018","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.aninu.2019.04.005","article-title":"Dietary supplementation of xylanase and protease on growth performance, digesta viscosity, nutrient digestibility, immune and oxidative stress status, and gut health of newly weaned pigs","volume":"5","author":"Duarte","year":"2019","journal-title":"Anim. Nutr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0377-8401(03)00163-9","article-title":"Review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals","volume":"108","author":"Montagne","year":"2003","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/S0271-5317(98)00048-7","article-title":"Fiber: Forms and functions","volume":"18","author":"Davidson","year":"1998","journal-title":"Nutr. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1079\/BJN2001416","article-title":"Increasing viscosity of the intestinal contents alters small intestinal structure and intestinal growth, and stimulates proliferation of enterotoxigenic Escherichia coli in newly-weaned pigs","volume":"86","author":"McDonald","year":"2001","journal-title":"Br. J. Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.2527\/2006.8461375x","article-title":"Intestinal morphology and enzymatic activity in newly weaned pigs fed contrasting fiber concentrations and fiber properties","volume":"84","author":"Hedemann","year":"2006","journal-title":"J. Anim. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1079\/BJN20041206","article-title":"Addition of pearl barley to a rice-based diet for newly weaned piglets increases the viscosity of the intestinal contents, reduces starch digestibility and exacerbates post-weaning colibacillosis","volume":"92","author":"Hopwood","year":"2004","journal-title":"Br. J. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1016\/j.foodchem.2011.02.042","article-title":"Non-starch polysaccharides and their role in fish nutrition\u2014A review","volume":"127","author":"Sinha","year":"2011","journal-title":"Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1038\/265623a0","article-title":"Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors","volume":"265","author":"Ofek","year":"1977","journal-title":"Nature"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.anifeedsci.2004.03.010","article-title":"Influence of cereal non-starch polysaccharides and enzyme supplementation on digestion site and gut environment in weaned piglets","volume":"116","author":"Lindberg","year":"2004","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1080\/09712119.2017.1358628","article-title":"The microbial pH-stable exogenous multienzyme improved growth performance and intestinal morphology of weaned pigs fed a corn\u2013soybean-based diet","volume":"46","author":"Kim","year":"2018","journal-title":"J. Appl. Anim. Res."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/4\/703\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:21:21Z","timestamp":1760361681000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/4\/703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,17]]},"references-count":39,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["ani10040703"],"URL":"https:\/\/doi.org\/10.3390\/ani10040703","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,17]]}}}