{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:30:36Z","timestamp":1792441836028,"version":"4.5.0"},"reference-count":54,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T00:00:00Z","timestamp":1588982400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Therapy dogs are increasingly being incorporated into numerous clinical settings. However, there are only a handful of studies that have focused on the impact of animal-assisted activity or therapy sessions on the wellbeing of the therapy dogs. Furthermore, these studies show mixed results. The goal of this study was to provide an in-depth picture of the effects of these interactions on the dogs involved by considering multiple physiological measures known to be associated with emotional state (continuous heart rate, heart rate variability, pre- and post-session tympanic membrane temperatures, and salivary cortisol and oxytocin concentrations). Nineteen Mayo Clinic Caring Canine therapy dogs completed five 20-minute animal-assisted activity (AAA) visits each in an outpatient clinical setting (Mayo Clinic Fibromyalgia and Chronic Fatigue Clinic). From a physiological perspective, the dogs showed a neutral to positive response to the AAA sessions. Heart rate (HR) was significantly lower at the end of the session compared with the beginning of the session (F = 17.26, df1 = 1, df2 = 29.7, p = 0.0003). The right tympanic membrane temperature was lower post-session (F = 8.87, df1 = 1, df2 = 107, p = 0.003). All other emotional indicators remained stable between pre- and post-session. These results suggest that the dogs involved were not negatively affected by their participation in the AAA. Moreover, there was some evidence suggesting the dogs may have been in a more relaxed state at the end of the session (lower HR and lower right tympanic membrane temperature) compared to the beginning of the session.<\/jats:p>","DOI":"10.3390\/ani10050819","type":"journal-article","created":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T12:26:30Z","timestamp":1589199990000},"page":"819","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Physiological State of Therapy Dogs during Animal-Assisted Activities in an Outpatient Setting"],"prefix":"10.3390","volume":"10","author":[{"given":"Stephanie D.","family":"Clark","sequence":"first","affiliation":[{"name":"Section of Integrative Medicine and Health, Division of General Internal Medicine, Mayo Clinic, Rochester, MN 55901, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Martin","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Purina Research, St. Louis, MO 63102, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ragen T.S.","family":"McGowan","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Purina Research, St. Louis, MO 63102, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica M.","family":"Smidt","sequence":"additional","affiliation":[{"name":"Section of Integrative Medicine and Health, Division of General Internal Medicine, Mayo Clinic, Rochester, MN 55901, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Anderson","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Purina Research, St. Louis, MO 63102, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Purina Research, St. Louis, MO 63102, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tricia","family":"Turpin","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Purina Research, St. Louis, MO 63102, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Langenfeld-McCoy","sequence":"additional","affiliation":[{"name":"Nestl\u00e9 Purina Research, St. Louis, MO 63102, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent A.","family":"Bauer","sequence":"additional","affiliation":[{"name":"Section of Integrative Medicine and Health, Division of General Internal Medicine, Mayo Clinic, Rochester, MN 55901, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1737-4714","authenticated-orcid":false,"given":"Arya B.","family":"Mohabbat","sequence":"additional","affiliation":[{"name":"Section of Integrative Medicine and Health, Division of General Internal Medicine, Mayo Clinic, Rochester, MN 55901, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,9]]},"reference":[{"key":"ref_1","unstructured":"IAHAIO (2020, April 01). The IAHAIO Definitions for Animal Assisted Intervention and Guidelines for Wellness of Animals Involved in AAI. Available online: https:\/\/iahaio.org\/wp\/wp-content\/uploads\/2018\/04\/iahaio_wp_updated-2018-final.pdf."},{"key":"ref_2","unstructured":"Pet Partners (2020, April 01). Position Statements on Therapy Animal Health and Welfare. Available online: https:\/\/petpartners.org\/learn\/position-statements\/."},{"key":"ref_3","unstructured":"AVMA (2020, April 01). Animal-Assisted Interventions: Guidelines. Available online: https:\/\/www.avma.org\/policies\/animal-assisted-interventions-guidelines."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1080\/13576500802344420","article-title":"The right ear but not the left ear temperature is related to stress-induced cortisolaemia in the domestic cat (Felis catus)","volume":"14","author":"Mazzotti","year":"2009","journal-title":"Laterality"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Riemer, S., Assis, L., Pike, T.W., and Mills, D.S. (2016). Dynamic changes in ear temperature in relation to separation distress in dogs. Physiol. Behav.","DOI":"10.1016\/j.physbeh.2016.09.002"},{"key":"ref_6","first-page":"26","article-title":"Welfare considerations in therapy and assistance animals","volume":"Volume 23","author":"Fine","year":"2010","journal-title":"Handbook on Animal-Assisted Therapy: Theoretical Foundations and Guidelines for Practice"},{"key":"ref_7","first-page":"48","article-title":"Animal-assisted therapy and the severely disabled child: A quantitative study","volume":"67","author":"Heimlich","year":"2001","journal-title":"J. Rehab."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Glenk, L.M. (2017). Current perspectives on therapy dog welfare in animal-assisted interventions. Animals, 7.","DOI":"10.3390\/ani7020007"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"S93","DOI":"10.1007\/s11259-009-9256-x","article-title":"Dog assisted interventions in a specialized center and potential concerns for animal welfare","volume":"33","author":"Marinelli","year":"2009","journal-title":"Vet. Res. Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.jveb.2011.01.001","article-title":"Evaluation of physiological and behavioral stress-dependent parameters in agility dogs","volume":"6","author":"Pastore","year":"2011","journal-title":"J. Vet Behav."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.jveb.2018.12.002","article-title":"Welfare consideration: Salivary cortisol concentrations on frequency of therapy dog visits in an outpatient hospital setting: A pilot study","volume":"30","author":"Clark","year":"2018","journal-title":"J. Vet. Behav."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.applanim.2017.11.014","article-title":"Physiological and behavioral effects of animal-assisted interventions on therapy dogs in pediatric oncology settings","volume":"200","author":"McCullough","year":"2018","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Melco, A.L., Goldman, L., Fine, A.H., and Peralta, J.M. (2020). Investigation of physiological and behavioral responses in dogs participating in animal-assisted therapy with children diagnosed with attention-deficit hyperactivity disorder. J. Appl. Anim. Welf. Sci.","DOI":"10.1080\/10888705.2018.1536979"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.jveb.2011.01.007","article-title":"Effect of a time-out session with working animal-assisted therapy dogs","volume":"6","author":"King","year":"2011","journal-title":"J. Vet. Behav."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Palestrini, C., Calcaterra, V., Cannas, S., Talamonti, Z., Papotti, F., Buttram, D., and Pelizzo, G. (2016). Stress level evaluation in a dog during animal-assisted therapy in pediatric surgery. J. Vet. Behav.","DOI":"10.1016\/j.jveb.2016.09.003"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1207\/s15327604jaws0902_5","article-title":"Physiological arousal for companion dogs working with their owners in animal-assisted activities and animal-assisted therapy","volume":"9","author":"Haubenhofer","year":"2006","journal-title":"J. Appl. Anim. Welf. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"de Carvalho, I.R., Nunes, T., de Sousa, L., and Almeida, V. (2019). The combined use of salivary cortisol concentrations, heart rate and respiratory rate for the welfare assessment of dogs involved in AAI programs. J. Vet. Behav.","DOI":"10.1016\/j.jveb.2019.10.011"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Clark, S.D., Martin, F., McGowan, R.T.S., Smidt, J.M., Anderson, A., Wang, L., Turpin, T., Langenfeld-McCoy, N., Bauer, B.A., and Mohabbat, A.B. (2020). The Impact of a 20-Minute Animal-Assisted Activity Session on the Physiological and Emotional States in Patients with Fibromyalgia. Mayo Clinic Proc., Accepted for publication.","DOI":"10.1016\/j.mayocp.2020.04.037"},{"key":"ref_19","first-page":"50","article-title":"Raw food diets in companion animals: A critical review","volume":"52","author":"Schlesinger","year":"2011","journal-title":"Can. Vet. J."},{"key":"ref_20","unstructured":"AVMA (2019, September 05). Raw Pet Foods and the AVMA\u2019s Policy: FAQ. Available online: https:\/\/www.avma.org\/KB\/Resources\/FAQs\/Pages\/Raw-Pet-Foods-and-the-AVMA-Policy-FAQ.aspx."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1097\/HRP.0000000000000024","article-title":"Learning through the lens: Ethical considerations in videotaping psychotherapy","volume":"22","author":"Funkenstein","year":"2014","journal-title":"Harvard. Rev. Psych."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1258\/ce.2011.011040","article-title":"Ongoing processes of managing consent: The empirical ethics of using video-recording in clinical practice and research","volume":"6","author":"Parker","year":"2011","journal-title":"Clin. Ethics."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Wang, L., Marti, D.W., and Anderson, R.E. (2019). Development and validation of a simple LC-MS a method for the quantification of oxytocin in dog saliva. Molecules, 24.","DOI":"10.3390\/molecules24173079"},{"key":"ref_24","unstructured":"R Core Team (2019, September 09). R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. Available online: https:\/\/www.R-project.org\/."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"199","DOI":"10.2752\/175303712X13316289505422","article-title":"Effects of owner-dog relationship and owner personality on cortisol modulation in human dog dyads","volume":"25","author":"Wedl","year":"2012","journal-title":"Anthrozoo"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.jveb.2015.09.007","article-title":"Social factors influencing cortisol modulation in dogs during a strange situation procedure","volume":"11","author":"Beetz","year":"2016","journal-title":"J. Vet. Behav."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.applanim.2018.02.011","article-title":"Can you spare 15 minutes? The measurable positive impact of a 15-min petting session on shelter dog well-being","volume":"203","author":"McGowan","year":"2018","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jveb.2014.03.002","article-title":"Dog and owner characteristics affecting the dog owner relationship","volume":"9","author":"Meyer","year":"2014","journal-title":"J. Vet. Behav."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.domaniend.2016.04.003","article-title":"A systematic review and meta-analysis of salivary cortisol measurement in domestic canines","volume":"57","author":"Cobb","year":"2016","journal-title":"Dom. Anim. Endocrinol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"301","DOI":"10.2752\/175303711X13045914865385","article-title":"Short-term interaction between dogs and their owners: Effects on oxytocin, cortisol, insulin and heart rate\u2014An exploratory study","volume":"24","author":"Handlin","year":"2011","journal-title":"Anthrozo\u00f6s"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/S1090-0233(02)00237-X","article-title":"Neurophysiological correlates of affiliative behavior between humans and dogs","volume":"165","author":"Odendaal","year":"2003","journal-title":"Vet. J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.37358\/RC.19.5.7172","article-title":"Conceptual replication of canine serum oxytocin increase following a positive dog-human interaction","volume":"70","author":"Hritcu","year":"2019","journal-title":"Rev. Chim."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.applanim.2012.02.006","article-title":"Measuring empathic responses in animals","volume":"138","author":"Edgar","year":"2012","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.psyneuen.2008.10.026","article-title":"Salivary cortisol as a biomarker in stress research","volume":"34","author":"Hellhammer","year":"2008","journal-title":"Psychoneuroendocrinology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"363","DOI":"10.17221\/19\/2017-VETMED","article-title":"Analysis of cortisol in dog hair A potential biomarker of chronic stress: A review","volume":"62","author":"Mesacova","year":"2017","journal-title":"Vet. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.jveb.2008.09.022","article-title":"Welfare assessment of dogs involved in animal assisted activities","volume":"4","author":"Marinelli","year":"2009","journal-title":"J. Vet. Behav. Clin. App. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.applanim.2014.07.009","article-title":"The effect of dog\u2013human interaction on cortisol and behavior in registered animal-assisted activity dogs","volume":"159","author":"Ng","year":"2014","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"369","DOI":"10.7120\/09627286.22.3.369","article-title":"Therapy dogs\u2019 salivary cortisol levels vary during animal-assisted interventions","volume":"22","author":"Glenk","year":"2013","journal-title":"Anim. Welf."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.jveb.2014.02.005","article-title":"Salivary cortisol and behavior in therapy dogs during animal-assisted interventions: A pilot study","volume":"9","author":"Glenk","year":"2014","journal-title":"J. Vet. Behav."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1016\/j.psyneuen.2013.06.019","article-title":"The role of oxytocin in social bonding, stress regulation, and mental health: An update on the moderating effects of context and inter individual differences","volume":"38","author":"Olff","year":"2013","journal-title":"Psychoneuroendocrinology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"234","DOI":"10.3389\/fpsyg.2012.00234","article-title":"Psychosocial and psychophysiological effects of human-animal interactions: The possible role of oxytocin","volume":"3","author":"Beetz","year":"2012","journal-title":"Front. Psychol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.3389\/fpsyg.2017.01613","article-title":"Endogenous oxytocin, vasopressin, and aggression in domestic dogs","volume":"8","author":"MacLean","year":"2017","journal-title":"Front. Psychol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.physbeh.2013.10.009","article-title":"Dogs\u2019 endocrine and behavioral responses at reunion are affected by how the human initiates contact","volume":"124","author":"Rehn","year":"2014","journal-title":"Physiol. Behav."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1037\/0735-7044.112.1.83","article-title":"Lateralized changes in tympanic membrane temperature in relation to difference cognitive tasks in Chimpanzees (Pan troglodytes)","volume":"112","author":"Hopkins","year":"1998","journal-title":"Behav. Neurosci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.applanim.2010.06.008","article-title":"Relevance of brain and behavioural lateralization to animal welfare","volume":"127","author":"Rogers","year":"2010","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.physbeh.2016.06.020","article-title":"Stress and laterality The comparative perspective","volume":"164","author":"Ocklenberg","year":"2016","journal-title":"Physiol. Behav."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-017-18417-4","article-title":"Lateralized behavior and cardiac activity of dogs in response to human emotional vocalizations","volume":"8","author":"Siniscalchi","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.jveb.2014.02.006","article-title":"Behavioral and cardiac responses by dogs to physical human-dog contact","volume":"9","author":"Kuhne","year":"2014","journal-title":"J. Vet. Behav."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Varga, B., Gergely, A., Galambos, A., and Kis, A. (2018). Heart rate and heart rate variability during sleep in family dogs (Canis familiaris). Moderate effect of pre-sleep emotions. Animals, 8.","DOI":"10.3390\/ani8070107"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1037\/1089-2680.10.3.229","article-title":"Heart rate variability as an index of regulated emotional responding","volume":"10","author":"Appelhans","year":"2006","journal-title":"Rev. Gen. Psychol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.jfms.2004.12.003","article-title":"Heart rate and heart rate variability in healthy cats at home and in hospital environments","volume":"7","author":"Abbott","year":"2004","journal-title":"J. Feline Med. Surg."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.beproc.2016.04.015","article-title":"Heart rate variability predicts the emotional state in dogs","volume":"128","author":"Katayama","year":"2016","journal-title":"Behav. Proc."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.applanim.2005.02.005","article-title":"Heart rate and behavioral response of dogs in Ainsworth\u2019s Strange Situation: A pilot study","volume":"94","author":"Palestrini","year":"2005","journal-title":"Appl. Anim. Behav. Sci."},{"key":"ref_54","unstructured":"Larsen, H.R. (2019, September 09). Heart Rate Variability and Atrial Fibrillation. Available online: http:\/\/afibbers.org\/resources\/HRV.pdf."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/5\/819\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:27:02Z","timestamp":1760174822000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/5\/819"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,9]]},"references-count":54,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["ani10050819"],"URL":"https:\/\/doi.org\/10.3390\/ani10050819","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,9]]}}}