{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T11:39:57Z","timestamp":1782819597854,"version":"3.54.5"},"reference-count":38,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T00:00:00Z","timestamp":1589328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Economy and Competitiveness (Spanish Government) co-financed by FEDER funds","award":["AGL2016-76568-R"],"award-info":[{"award-number":["AGL2016-76568-R"]}]},{"name":"State Subprogram Training of the State Program for the Promotion of Talent and its Employability","award":["BES-2017-080186"],"award-info":[{"award-number":["BES-2017-080186"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Mycoplasma bovis is an important etiologic agent of bovine mycoplasmosis in cattle. Different transmission routes have been described, including those related to reproduction. The presence of mycoplasma in semen has led to its appearance in infection-free areas through artificial insemination (AI). Semen was recently reported to be the initial source of two M. bovis mastitis outbreaks in two closed dairy herds in Finland. This questions the effectiveness of the antimicrobials currently used in semen extenders to control the pathogens in contaminated semen. They should be re-evaluated, or alternative measures to antimicrobials should be tested to obtain M. bovis-free semen. This in vitro study aimed to assess different strategies to reduce the risk of transmission of M. bovis through AI technologies. The viability of M. bovis (PG45, NCTC 10131) in bull semen diluted (DS) in a Tris-citrate-fructose solution was tested, after the addition of enrofloxacin, doxycycline or a Lactobacillus spp.-based probiotic. The data show the susceptibility of the pathogen to the addition of 0.125 \u03bcg\/mL of enrofloxacin or 0.0625 \u03bcg\/mL of doxycycline and to the addition of the probiotic at a concentration of 3.24 \u00d7 106 colony forming units (CFU)\/mL or 3.24 \u00d7 108 CFU\/mL in DS. The Tris-citrate-fructose medium negatively affected the viability of M. bovis, although this effect was lower than that observed after the addition of the probiotic and antimicrobials (p &lt; 0.05). Our results may support new strategies for reducing the risk of M. bovis transmission through AI.<\/jats:p>","DOI":"10.3390\/ani10050837","type":"journal-article","created":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T02:55:41Z","timestamp":1589424941000},"page":"837","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Addition of Lactobacillus spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of Mycoplasma bovis in Diluted Bovine Semen"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5613-2975","authenticated-orcid":false,"given":"Ana","family":"Garc\u00eda-Gal\u00e1n","sequence":"first","affiliation":[{"name":"Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia,  30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1ngel","family":"G\u00f3mez-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia,  30100 Murcia, Spain"},{"name":"Microbiological Agents Associated with Animal Reproduction (ProVaginBio) Research Group, Department of Animal Health and Public Health, Faculty of Veterinary Sciences, University CEU Cardenal Herrera of Valencia, CEU Universities,  46113 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Bataller","sequence":"additional","affiliation":[{"name":"Microbiological Agents Associated with Animal Reproduction (ProVaginBio) Research Group, Department of Animal Health and Public Health, Faculty of Veterinary Sciences, University CEU Cardenal Herrera of Valencia, CEU Universities,  46113 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Gomis","sequence":"additional","affiliation":[{"name":"Microbiological Agents Associated with Animal Reproduction (ProVaginBio) Research Group, Department of Animal Health and Public Health, Faculty of Veterinary Sciences, University CEU Cardenal Herrera of Valencia, CEU Universities,  46113 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1868-3829","authenticated-orcid":false,"given":"Antonio","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia,  30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3474-7626","authenticated-orcid":false,"given":"Joaqu\u00edn","family":"Gadea","sequence":"additional","affiliation":[{"name":"Physiology of Reproduction Research Group, Department of Physiology, Faculty of Veterinary Sciences, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia,  30100 Murcia, Spain"},{"name":"Institute for Biomedical Research of Murcia IMIB-Arrixaca,  30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2419-1329","authenticated-orcid":false,"given":"Luis Alberto","family":"Vieira","sequence":"additional","affiliation":[{"name":"Physiology of Reproduction Research Group, Department of Physiology, Faculty of Veterinary Sciences, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia,  30100 Murcia, Spain"},{"name":"Institute for Biomedical Research of Murcia IMIB-Arrixaca,  30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9319-0277","authenticated-orcid":false,"given":"Empar","family":"Garc\u00eda-Rosell\u00f3","sequence":"additional","affiliation":[{"name":"Microbiological Agents Associated with Animal Reproduction (ProVaginBio) Research Group, Department of Animal Health and Public Health, Faculty of Veterinary Sciences, University CEU Cardenal Herrera of Valencia, CEU Universities,  46113 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"De la Fe","sequence":"additional","affiliation":[{"name":"Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia,  30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0034-5288(02)00155-8","article-title":"Mycoplasma bovis: Disease, diagnosis, and control","volume":"74","author":"Nicholas","year":"2003","journal-title":"Res. 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