{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T05:12:38Z","timestamp":1776921158524,"version":"3.51.2"},"reference-count":72,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T00:00:00Z","timestamp":1589932800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This work was supported by statutory funding No. 506.533.04.00 of the Faculty of Veterinary Medicine and Animal Science Poznan University of Life Sciences, Poland; Department of Animal Nutrition.","award":["No. 506.533.04.00"],"award-info":[{"award-number":["No. 506.533.04.00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The aim of the study was to investigate the effect of Bacillus licheniformis and salinomycin supplementation in broiler diets as individual factors or in combination on the growth performance, GIT morphometry, and microbiota populations. Four hundred one-day-old Ross 308 chicks were randomly distributed to four dietary treatments (10 replicates, 10 birds each). The following treatments were applied: NC\u2014no additives; NC + SAL\u2014salinomycin addition (60 mg\/kg diet), NC + PRO\u2014B. licheniformis DSM 28710 preparation (1.6 \u00d7 109 CFU\/kg; 500 mg\/kg diet), and NC + SAL + PRO\u2014combination of salinomycin and B. licheniformis. Probiotic administration resulted in improvement (p &lt; 0.05) of the performance parameters, including body weight gain (1\u201310 d, and 11\u201322 d) and feed conversion ratio (11\u201322 d, 1\u201336 d). An interaction (p &lt; 0.05) between experimental factors was observed in terms of lower pH values in the crop (tendency, p = 0.053) and ceca. Both factors lowered the alpha diversity and Enterobacteriaceae and promoted Bacillaceae communities in the jejunum (p &lt; 0.05). Interactions were also observed in terms of reducing Clostridiaceae in the ceca. In conclusion, the combined use of B. licheniformis and salinomycin in broilers\u2019 diets had beneficial effects.<\/jats:p>","DOI":"10.3390\/ani10050889","type":"journal-article","created":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T10:37:38Z","timestamp":1589971058000},"page":"889","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Combination of Bacillus licheniformis and Salinomycin: Effect on the Growth Performance and GIT Microbial Populations of Broiler Chickens"],"prefix":"10.3390","volume":"10","author":[{"given":"Jacek","family":"Trela","sequence":"first","affiliation":[{"name":"Huvepharma Poland Sp. z.o.o., ul. Aleje Jerozolimskie 146D, 02-305 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6006-117X","authenticated-orcid":false,"given":"Bartosz","family":"Kiero\u0144czyk","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, Pozna\u0144 University of Life Sciences, Wo\u0142y\u0144ska 33, 60-637 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veerle","family":"Hautekiet","sequence":"additional","affiliation":[{"name":"Huvepharma NV, Uitbreidingstraat 80, 2600 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damian","family":"J\u00f3zefiak","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, Pozna\u0144 University of Life Sciences, Wo\u0142y\u0144ska 33, 60-637 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10611","DOI":"10.1007\/s11356-018-1687-x","article-title":"The use of probiotics as eco-friendly alternatives for antibiotics in poultry nutrition","volume":"25","author":"Alagawany","year":"2018","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"48","DOI":"10.29252\/JABR.05.02.02","article-title":"A review of dietary probiotics in poultry","volume":"5","author":"Mousavi","year":"2018","journal-title":"J. Appl. Biotechnol. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Anad\u00f3n, A., Ares, I., Mart\u00ednez-Larra\u00f1aga, M.R., and Mart\u00ednez, M.A. (2019). Prebiotics and probiotics in feed and animal health. Nutraceuticals in Veterinary Medicine, Springer Nature Switzerland AG.","DOI":"10.1007\/978-3-030-04624-8_19"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4026","DOI":"10.3382\/ps\/pex234","article-title":"The physiological response of broiler chickens to the dietary supplementation of the bacteriocin nisin and ionophore coccidiostats","volume":"96","author":"Sassek","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kiero\u0144czyk, B., Rawski, M., Miko\u0142ajczak, Z., \u015awi\u0105tkiewicz, S., and J\u00f3zefiak, D. (2020). Nisin as a novel feed additive: The effects on gut microbial modulation and activity, histological parameters, and growth performance of broiler chickens. Animals, 10.","DOI":"10.3390\/ani10010101"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"17","DOI":"10.4103\/2229-5186.94308","article-title":"Promising future of probiotics for human health: Current scenario","volume":"3","author":"Tiwari","year":"2012","journal-title":"Chron. Young Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Hejdysz, M., Kaczmarek, S.A., Kubi\u015b, M., Wi\u015bniewska, Z., Peris, S., Budnik, S., and Rutkowski, A. (2020). The effect of protease and Bacillus licheniformis on nutritional value of pea, faba bean, yellow lupin and narrow-leaved lupin in broiler chicken diets. Br. Poult. Sci.","DOI":"10.1080\/00071668.2020.1716303"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4722","DOI":"10.3382\/ps\/pez184","article-title":"Efficacy of dietary Bacillus subtilis and Bacillus licheniformis supplementation continuously in pullet and lay period on egg production, excreta microflora, and egg quality of Hyline-Brown birds","volume":"98","author":"Upadhaya","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.3382\/ps\/pey478","article-title":"Bacillus amyloliquefaciens spray improves the growth performance, immune status, and respiratory mucosal barrier in broiler chickens","volume":"98","author":"Luan","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s40104-017-0220-2","article-title":"Effects of Bacillus coagulans supplementation on the growth performance and gut health of broiler chickens with Clostridium perfringens-induced necrotic enteritis","volume":"9","author":"Wu","year":"2018","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1637\/9106-101509-ResNote.1","article-title":"Bacillus licheniformis prevents necrotic enteritis in broiler chickens","volume":"54","author":"Knap","year":"2010","journal-title":"Avian Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s12944-016-0219-2","article-title":"Effects of Bacillus licheniformis on the growth performance and expression of lipid metabolism-related genes in broiler chickens challenged with Clostridium perfringens-induced necrotic enteritis","volume":"15","author":"Zhou","year":"2016","journal-title":"Lipids Health Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"682","DOI":"10.5713\/ajas.2011.11334","article-title":"Growth performance and meat quality of broiler chickens supplemented with Bacillus licheniformis in drinking water","volume":"25","author":"Liu","year":"2012","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1111\/j.1574-6968.2001.tb10951.x","article-title":"Secretion of a trypsin-like thiol protease by a new keratinolytic strain of Bacillus licheniformis","volume":"205","author":"Rozs","year":"2001","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Robinson, K., Becker, S., Xiao, Y., Lyu, W., Yang, Q., Zhu, H., Yang, H., Zhao, J., and Zhang, G. (2019). Differential impact of subtherapeutic antibiotics and ionophores on intestinal microbiota of broilers. Microorganisms, 7.","DOI":"10.3390\/microorganisms7090282"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3104","DOI":"10.3382\/ps\/pex114","article-title":"The sub-inhibitory theory for antibiotic growth promoters","volume":"96","author":"Broom","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.ejmech.2019.01.035","article-title":"Anti-parasitic activity of polyether ionophores","volume":"166","author":"Antoszczak","year":"2019","journal-title":"Eur. J. Med. Chem."},{"key":"ref_18","first-page":"51","article-title":"Comparison of the effect of probiotic, prebiotic, salinomycin and vaccine in control of coccidiosis in broiler chickens","volume":"20","author":"Behnamifar","year":"2019","journal-title":"Iran. J. Vet. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"57","DOI":"10.2141\/jpsa.011031","article-title":"Effect of dietary inclusion of probiotic, prebiotic and butyric acid glycerides on resistance against coccidiosis in broiler chickens","volume":"49","author":"Taherpour","year":"2012","journal-title":"J. Poult. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"309","DOI":"10.22358\/jafs\/67802\/2016","article-title":"The nisin improves broiler chicken growth performance and interacts with salinomycin in terms of gastrointestinal tract microbiota composition","volume":"25","author":"Rawski","year":"2016","journal-title":"J. Anim. Feed Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1080\/1745039X.2012.663668","article-title":"Effects of sodium butyrate and salinomycin upon intestinal microbiota, mucosal morphology and performance of broiler chickens","volume":"66","author":"Smulikowska","year":"2012","journal-title":"Arch. Anim. Nutr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"6566","DOI":"10.1128\/AEM.01086-07","article-title":"Impact of feed supplementation with antimicrobial agents on growth performance of broiler chickens, Clostridium perfringens and Enterococcus counts, and antibiotic resistance phenotypes and distribution of antimicrobial resistance determinants in Escheric","volume":"73","author":"Diarra","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","unstructured":"(2020, April 15). Available online: http:\/\/eu.aviagen.com\/assets\/Tech_Center\/Ross_Broiler\/Ross-BroilerHandbook2018-EN.pdf."},{"key":"ref_24","unstructured":"Council, N.R. (1994). Nutrient Requirements of Poultry: 1994, National Academies Press."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1093\/ps\/83.3.414","article-title":"Effects of dietary protein source and level on intestinal populations of Clostridium perfringens in broiler chickens","volume":"83","author":"Drew","year":"2004","journal-title":"Poult. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5918","DOI":"10.1128\/AEM.68.12.5918-5924.2002","article-title":"Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at various ages","volume":"68","author":"Knarreborg","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1111\/j.1574-695X.1999.tb01303.x","article-title":"Necrotic enteritis challenge models with broiler chickens raised on litter: Evaluation of preconditions, Clostridium perfringens strains and outcome variables","volume":"24","author":"Kaldhusdal","year":"1999","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Rychen, G., Aquilina, G., Azimonti, G., Bampidis, V., de Lourdes Bastos, M., Bories, G., Chesson, A., Cocconcelli, P.S., Flachowsky, G., and Gropp, J. (2016). Safety and efficacy of B-Act\u00ae (Bacillus licheniformis DSM 28710) for chickens for fattening and chickens reared for laying. EFSA J., 14.","DOI":"10.2903\/j.efsa.2016.4615"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5069","DOI":"10.1128\/AEM.03006-05","article-title":"Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB","volume":"72","author":"DeSantis","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search and clustering orders of magnitude faster than BLAST","volume":"26","author":"Edgar","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1101\/gr.112730.110","article-title":"Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons","volume":"21","author":"Haas","year":"2011","journal-title":"Genome Res."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Schumacker, R., and Tomek, S. (2013). R Fundamentals BT\u2014Understanding Statistics Using R, Springer.","DOI":"10.1007\/978-1-4614-6227-9"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"De Mendiburu, F., and Simon, R. (2015). Agricolae-Ten years of an open source statistical tool for experiments in breeding, agriculture and biology. PeerJ.","DOI":"10.7287\/peerj.preprints.1404v1"},{"key":"ref_36","unstructured":"Revelle, W. (2020, April 15). An introduction to the psych package: Part I: Data entry and data description 2017. Available online: http:\/\/www.personality-project.org\/r\/tutorials\/vignettes\/intro.pdf."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Mailund, T. (2019). Manipulating Data Frames: Dplyr. R Data Science Quick Reference, Apress.","DOI":"10.1007\/978-1-4842-4894-2"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Ogle, D.H. (2016). Introductory Fisheries Analyses with R, CRC Press.","DOI":"10.1201\/b19232"},{"key":"ref_39","unstructured":"Rasco, D. (2020). An R Companion for Applied Statistics I: Basic Bivariate Techniques, SAGE Publications."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Wickham, H. (2016). Ggplot2: Elegant Graphics for Data Analysis, Springer.","DOI":"10.1007\/978-3-319-24277-4_9"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1111\/asj.13089","article-title":"Effects of three probiotic Bacillus on growth performance, digestive enzyme activities, antioxidative capacity, serum immunity, and biochemical parameters in broilers","volume":"89","author":"Gong","year":"2018","journal-title":"Anim. Sci. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1111\/jpn.13082","article-title":"Bacillus subtilis B21 and Bacillus licheniformis B26 improve intestinal health and performance of broiler chickens with Clostridium perfringens-induced necrotic enteritis","volume":"103","author":"Musa","year":"2019","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2389","DOI":"10.3382\/ps.2012-02686","article-title":"Influence of dietary inclusion of Bacillus licheniformis on laying performance, egg quality, antioxidant enzyme activities, and intestinal barrier function of laying hens","volume":"92","author":"Lei","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_44","first-page":"95","article-title":"Effect of Bacillus subtilis and Bacillus licheniformis inclusion in turkey diets on growth performance","volume":"57","author":"Hanuszewska","year":"2018","journal-title":"Ann. Wars. Univ. Life Sci. SGGW Anim. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1093\/ps\/79.9.1311","article-title":"Effect of zinc bacitracin and salinomycin on intestinal microflora and performance of broilers","volume":"79","author":"Engberg","year":"2000","journal-title":"Poult. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1079\/9781845931803.0124","article-title":"Microbes of the chicken gastrointestinal tract","volume":"28","author":"Apajalahti","year":"2006","journal-title":"Avian Gut Funct. Heal. Dis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1111\/jpn.13004","article-title":"Performance and intestinal microbiota of chickens receiving probiotic in the feed and submitted to antibiotic therapy","volume":"103","author":"Pereira","year":"2019","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.psj.2019.12.013","article-title":"Influential factors on the composition of the conventionally raised broiler gastrointestinal microbiomes","volume":"99","author":"Feye","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1515\/aoas-2016-0032","article-title":"Avian crop function\u2014A review","volume":"16","author":"Rawski","year":"2016","journal-title":"Ann. Anim. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1017\/S1466252312000138","article-title":"The microbiome of the chicken gastrointestinal tract","volume":"13","author":"Yeoman","year":"2012","journal-title":"Anim. Heal. Res. Rev."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3149","DOI":"10.1016\/j.foodres.2011.08.017","article-title":"The intestinal microbiota and its modulation for Salmonella control in chickens","volume":"44","author":"Chambers","year":"2011","journal-title":"Food Res. Int."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1038\/s41598-018-20059-z","article-title":"Bacillus licheniformis normalize the ileum microbiota of chickens infected with necrotic enteritis","volume":"8","author":"Xu","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.3389\/fphys.2018.01968","article-title":"Intestinal morphologic and microbiota responses to dietary Bacillus spp. in a broiler chicken model","volume":"9","author":"Li","year":"2019","journal-title":"Front. Physiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1016\/j.psj.2019.10.036","article-title":"Effects of encapsulated cinnamaldehyde and citral on the performance and cecal microbiota of broilers vaccinated or not vaccinated against coccidiosis","volume":"99","author":"Yang","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Kumar, S., Chen, C., Indugu, N., Werlang, G.O., Singh, M., Kim, W.K., and Thippareddi, H. (2018). Effect of antibiotic withdrawal in feed on chicken gut microbial dynamics, immunity, growth performance and prevalence of foodborne pathogens. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0192450"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3839","DOI":"10.3382\/ps\/pez096","article-title":"Effects of Bacillus subtilis and coccidial vaccination on cecal microbial diversity and composition of Eimeria-challenged male broilers","volume":"98","author":"Wang","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Ren, H., Vahjen, W., Dadi, T., Saliu, E.-M., Boroojeni, F.G., and Zentek, J. (2019). Synergistic Effects of probiotics and phytobiotics on the intestinal microbiota in young broiler chicken. Microorganisms, 7.","DOI":"10.3390\/microorganisms7120684"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"15358","DOI":"10.1038\/s41598-018-33762-8","article-title":"Supplemental Bacillus subtilis DSM 32315 manipulates intestinal structure and microbial composition in broiler chickens","volume":"8","author":"Ma","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"J\u00f3zefiak, A., Benzertiha, A., Kiero\u0144czyk, B., \u0141ukomska, A., Weso\u0142owska, I., and Rawski, M. (2020). Improvement of cecal commensal microbiome following the insect additive into chicken diet. Animals, 10.","DOI":"10.3390\/ani10040577"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"272","DOI":"10.3382\/ps.2012-02603","article-title":"Influence of penicillin on microbial diversity of the cecal microbiota in broiler chickens","volume":"92","author":"Singh","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1128\/AEM.03046-12","article-title":"Diet-induced alterations of host cholesterol metabolism are likely to affect the gut microbiota composition in hamsters","volume":"79","author":"Perdicaro","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Hang, I., Rinttila, T., Zentek, J., Kettunen, A., Alaja, S., Apajalahti, J., Harmoinen, J., de Vos, W.M., and Spillmann, T. (2012). Effect of high contents of dietary animal-derived protein or carbohydrates on canine faecal microbiota. BMC Vet. Res., 8.","DOI":"10.1186\/1746-6148-8-90"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1016\/j.psj.2019.10.061","article-title":"Bacillus licheniformis\u2014Fermented products improve growth performance and the fecal microbiota community in broilers","volume":"99","author":"Chen","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"627","DOI":"10.3390\/d5030627","article-title":"Untangling the genetic basis of fibrolytic specialization by Lachnospiraceae and Ruminococcaceae in diverse gut communities","volume":"5","author":"Biddle","year":"2013","journal-title":"Diversity"},{"key":"ref_65","unstructured":"Manoharan, M.S. (2010). Effect of coccidiostat and natustatim on intestinal microflora in caecum of broilers challenged with Eimeria analyzed using bacterial 16S rDNA tag-encoded Flx amplicon pyrosequencing. [Master\u2019s Thesis, Stephen F. Austin State University]."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"3380","DOI":"10.1128\/AEM.02300-10","article-title":"Influence of antimicrobial feed additives on broiler commensal posthatch gut microbiota development and performance","volume":"77","author":"Torok","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"851","DOI":"10.3382\/ps\/pev382","article-title":"Effect of different doses of coated butyric acid on growth performance and energy utilization in broilers","volume":"95","author":"Kaczmarek","year":"2016","journal-title":"Poult. Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.aninu.2016.08.003","article-title":"Studying the effect of formic acid and potassium diformate on performance, immunity and gut health of broiler chickens","volume":"2","author":"Ragaa","year":"2016","journal-title":"Anim. Nutr."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.anifeedsci.2016.05.004","article-title":"Interaction between chicken intestinal microbiota and protein digestion","volume":"221","author":"Apajalahti","year":"2016","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.3382\/ps\/pey112","article-title":"High-throughput sequencing reveals the effect of Bacillus subtilis CGMCC 1.921 on the cecal microbiota and gene expression in ileum mucosa of laying hens","volume":"97","author":"Guo","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/femsle\/fnv122","article-title":"Prebiotics and gut microbiota in chickens","volume":"362","author":"Pourabedin","year":"2015","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"3531","DOI":"10.3390\/ijms10083531","article-title":"The role of probiotics in the poultry industry","volume":"10","author":"Kabir","year":"2009","journal-title":"Int. J. Mol. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/5\/889\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:30:32Z","timestamp":1760175032000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/5\/889"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,20]]},"references-count":72,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["ani10050889"],"URL":"https:\/\/doi.org\/10.3390\/ani10050889","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,20]]}}}