{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T22:38:29Z","timestamp":1786747109568,"version":"build-2736575974"},"reference-count":38,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["Next-Generation BioGreen 21 Program (Project No. PJ01329201)"],"award-info":[{"award-number":["Next-Generation BioGreen 21 Program (Project No. PJ01329201)"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Streptococcus bovis (S. bovis) is one of the critical initiators of acute acidosis in ruminants. Therefore, we aimed to develop and characterize the endolysin LyJH307, which can lyse ruminal S. bovis. We tested the bactericidal activity of recombinant LyJH307 against S. bovis JB1 under a range of pH, temperature, NaCl, and metal ion concentrations. In silico analyses showed that LyJH307 has a modular design with a distinct, enzymatically active domain of the NLPC\/P60 superfamily at the N-terminal and a cell wall binding domain of the Zoocin A target recognition domain (Zoocin A_TRD) superfamily at the C-terminal. The lytic activity of LyJH307 against S. bovis JB1 was the highest at pH 5.5, and relatively higher under acidic, than under alkaline conditions. LyJH307 activity was also the highest at 39 \u00b0C, but was maintained between 25\u00b0C and 55\u00b0C. LyJH307 bactericidal action was retained under 0-500 mM NaCl. While the activity of LyJH307 significantly decreased on treatment with ethylenediaminetetraacetic acid (EDTA), it was only restored with supplementation of 10 mM Ca2+. Analyses of antimicrobial spectra showed that LyJH307 lysed Lancefield groups D (S. bovis group and Enterococcus faecalis) and H (S. sanguinis) bacteria. Thus, LyJH307 might help to prevent acute ruminal acidosis.<\/jats:p>","DOI":"10.3390\/ani10060963","type":"journal-article","created":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T09:19:27Z","timestamp":1591089567000},"page":"963","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Characterization of Endolysin LyJH307 with Antimicrobial Activity against Streptococcus bovis"],"prefix":"10.3390","volume":"10","author":[{"given":"Hanbeen","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyo Gun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8144-7928","authenticated-orcid":false,"given":"Inhyuk","family":"Kwon","sequence":"additional","affiliation":[{"name":"EASY BIO, Inc., Seoul 06253, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakyeom","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"E17","DOI":"10.3168\/jds.2006-478","article-title":"Ruminal acidosis in beef cattle: The current microbiological and nutritional outlook1, 2","volume":"90","author":"Nagaraja","year":"2007","journal-title":"J. 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