{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:51:02Z","timestamp":1790272262492,"version":"4.1.0"},"reference-count":70,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T00:00:00Z","timestamp":1591315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Blood sampling is often performed in laboratory mice. Sampling techniques have the potential to cause pain, distress and impact on lifetime cumulative experience. In spite of institutions commonly providing guidance to researchers on these methods, and the existence of published guidelines, no systematic evaluation of the evidence on this topic exists. A systematic search of Medline, Scopus, and Web of Science was performed, identifying 27 studies on the impact of recovery blood sample techniques on mouse welfare and sample quality. Studies were appraised for quality using the SYstematic Review Centre for Laboratory animal Experimentation (SYRCLE) risk of bias tool. In spite of an acceptable number of studies being located, few studies examined the same pairwise comparisons. Additionally, there was considerable heterogeneity in study design and outcomes, with many studies being at a high risk of bias. Consequently, results were synthesised using the Synthesis Without Meta-analysis (SWiM) reporting guidelines. Grading of Recommendations, Assessment, Development and Evaluation (GRADE) was utilised for assessment of certainty in the evidence. Due to the heterogeneity and GRADE findings, it was concluded that there was not enough high-quality evidence to make any recommendations on the optimal method of blood sampling. Future high-quality studies, with standardised outcome measures and large sample sizes, are required.<\/jats:p>","DOI":"10.3390\/ani10060989","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T06:34:16Z","timestamp":1591684456000},"page":"989","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The Impact of Common Recovery Blood Sampling Methods, in Mice (Mus Musculus), on Well-Being and Sample Quality: A Systematic Review"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9011-8296","authenticated-orcid":false,"given":"Alexandra L","family":"Whittaker","sequence":"first","affiliation":[{"name":"School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy Campus, Roseworthy, South Australia 5371, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6897-814X","authenticated-orcid":false,"given":"Timothy H","family":"Barker","sequence":"additional","affiliation":[{"name":"JBI, Faculty of Health and Medical Sciences, The University of Adelaide, South Australia 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1258\/la.2010.010011","article-title":"Sublingual and submandibular blood collection in mice: A comparison of effects on body weight, food consumption and tissue damage","volume":"44","author":"Heimann","year":"2010","journal-title":"Lab. Anim."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1177\/0023677214541438","article-title":"Manual versus automated blood sampling: Impact of repeated blood sampling on stress parameters and behavior in male nmri mice","volume":"48","author":"Teilmann","year":"2014","journal-title":"Lab. Anim."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/jat.727","article-title":"A good practice guide to the administration of substances and removal of blood, including routes and volumes","volume":"21","author":"Diehl","year":"2001","journal-title":"J. Appl. Toxicol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1258\/la.2008.007073","article-title":"Blood collection from the sublingual vein in mice and hamsters: A suitable alternative to retrobulbar technique that provides large volumes and minimizes tissue damage","volume":"43","author":"Heimann","year":"2009","journal-title":"Lab. Anim."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1038\/laban1005-39","article-title":"A rapid, simple, and humane method for submandibular bleeding of mice using a lancet","volume":"34","author":"Golde","year":"2005","journal-title":"Lab. Anim. (New York)"},{"key":"ref_6","unstructured":"Popesko, P., Rajitov\u00e1, V., and Hor\u00e1k, J. (1992). A Colour Atlas of the Anatomy of Small Laboratory Animals, Wolfe Publishing."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1177\/0023677213494366","article-title":"Validation of a refined technique for taking repeated blood samples from juvenile and adult mice","volume":"47","author":"Sadler","year":"2013","journal-title":"Lab. Anim."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/laban.738","article-title":"Effects of different blood collection methods on indicators of welfare in mice","volume":"44","author":"Tsai","year":"2015","journal-title":"Lab. Anim. (New York)"},{"key":"ref_9","first-page":"8","article-title":"Evaluation of saphenous venipuncture and modified tail-clip blood collection in mice","volume":"47","author":"Abatan","year":"2008","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.steroids.2018.03.004","article-title":"Comparison of blood sampling methods for plasma corticosterone measurements in mice associated with minimal stress-related artefacts","volume":"135","author":"Kim","year":"2018","journal-title":"Steroids"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1258\/002367795780740339","article-title":"Corticosterone, adrenal and spleen weight in mice after tail bleeding, and its effect on nearby animals","volume":"29","author":"Tuli","year":"1995","journal-title":"Lab. Anim."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1177\/0023677217741332","article-title":"A comparison of various methods of blood sampling in mice and rats: Effects on animal welfare","volume":"52","author":"Harikrishnan","year":"2018","journal-title":"Lab. Anim."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"449","DOI":"10.7120\/09627286.22.4.449","article-title":"Hormonal stress response of laboratory mice to conventional and minimally invasive bleeding techniques","volume":"22","author":"Voigt","year":"2013","journal-title":"Anim. Welf."},{"key":"ref_14","unstructured":"(2020, June 05). SYstematic Review Centre for Laboratory Animal Experimentation (SYRCLE) Database for Animal Intervention Studies. Available online: https:\/\/drive.google.com\/file\/d\/1O7KD-1vuzlM-k56wD7--XpGKBveAUhH-\/view."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1097\/XEB.0000000000000152","article-title":"The development of software to support multiple systematic review types: The Joanna Briggs institute system for the unified management, assessment and review of information (jbi sumari)","volume":"17","author":"Munn","year":"2019","journal-title":"Int. J. Evid. Based Healthc."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Moher, D., Liberati, A., Tetzlaff, J., Altman, D.G., and The, P.G. (2009). Preferred reporting items for systematic reviews and meta-analyses: The prisma statement. PLoS Med., 6.","DOI":"10.1371\/journal.pmed.1000097"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Hooijmans, C.R., Rovers, M.M., de Vries, R.B., Leenaars, M., Ritskes-Hoitinga, M., and Langendam, M.W. (2014). Syrcle\u2019s risk of bias tool for animal studies. BMC Med. Res. Methodol., 14.","DOI":"10.1186\/1471-2288-14-43"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"l6890","DOI":"10.1136\/bmj.l6890","article-title":"Synthesis without meta-analysis (swim) in systematic reviews: Reporting guideline","volume":"368","author":"Campbell","year":"2020","journal-title":"BMJ"},{"key":"ref_19","unstructured":"Higgins, J.P.T., and Green, S. (2011). Chapter 12: Interpreting results and drawing conclusions. Cochrane Handbook for Systematic Reviews of Interventions, Cochrane. Version 5.1.0 (Updated March 2011)."},{"key":"ref_20","unstructured":"Sch\u00fcnemann, H., Bro\u017cek, J., Guyatt, G., and Oxman, A. (2016, November 23). Handbook for Grading the Quality of Evidence and the Strength of Recommendations for Using the GRADE Approach (Updated October 2013). Available online: gdt.guidelinedevelopment.org\/app\/handbook\/handbook.html."},{"key":"ref_21","unstructured":"GRADE Working Group (2014). GRADEpro GDT, McMaster University."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1258\/la.2009.009017","article-title":"Quality of blood samples from the saphenous vein compared with the tail vein during multiple blood sampling of mice","volume":"44","author":"Aasland","year":"2010","journal-title":"Lab. Anim."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1258\/la.2008.007075","article-title":"Quality of plasma sampled by different methods for multiple blood sampling in mice","volume":"43","author":"Christensen","year":"2009","journal-title":"Lab. Anim."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1016\/S0031-9384(96)00307-1","article-title":"Repeated blood collection in the laboratory mouse by tail incision\u2014Modification of an old technique","volume":"60","author":"Durschlag","year":"1996","journal-title":"Physiol. Behav."},{"key":"ref_25","first-page":"202","article-title":"Clinical biochemistry parameters in c57bl\/6j mice after blood collection from the submandibular vein and retroorbital plexus","volume":"49","year":"2010","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1038\/laban0810-236","article-title":"Morbidity and mortality rates associated with serial bleeding from the superficial temporal vein in mice","volume":"39","author":"Forbes","year":"2010","journal-title":"Lab. Anim."},{"key":"ref_27","first-page":"76","article-title":"Effects on animal wellbeing and sample quality of 2 techniques for collecting blood from the facial vein of mice","volume":"54","author":"Francisco","year":"2015","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_28","first-page":"317","article-title":"Type, duration, and incidence of pathologic findings after retroorbital bleeding of mice by experienced and novice personnel","volume":"54","author":"Fried","year":"2015","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"382","DOI":"10.30802\/AALAS-JAALAS-17-000134","article-title":"Comparison of serial blood collection by facial vein and retrobulbar methods in c57bl\/6 mice","volume":"57","author":"Frohlich","year":"2018","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"106680","DOI":"10.1016\/j.vascn.2020.106680","article-title":"Comparison of sublingual, facial and retro-bulbar blood sampling in mice in relation to animal welfare and blood quality","volume":"103","author":"Gjendal","year":"2020","journal-title":"J. Pharmacol. Toxicol. Methods"},{"key":"ref_31","first-page":"215","article-title":"Blood sampling from the tail vein, in comparison with two other techniques, causes less stress to mice","volume":"36","author":"Madetoja","year":"2009","journal-title":"Scand. J. Lab. Anim. Sci."},{"key":"ref_32","first-page":"307","article-title":"Comparing phlebotomy by tail tip amputation, facial vein puncture, and tail vein incision in c57bl\/6 mice by using physiologic and behavioral metrics of pain and distress","volume":"56","author":"Moore","year":"2017","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_33","first-page":"570","article-title":"Comparison of submental blood collection with the retroorbital and submandibular methods in mice (mus musculus)","volume":"55","author":"Regan","year":"2016","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_34","first-page":"25","article-title":"Influence of blood collection sites on plasma glucose and insulin concentration in conscious c57bl\/6 mice","volume":"38","author":"Rogers","year":"1999","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_35","first-page":"345","article-title":"Validation studies on blood collection from the jugular vein of conscious mice","volume":"51","author":"Shirasaki","year":"2012","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"362","DOI":"10.30802\/AALAS-JAALAS-18-000064","article-title":"Time-dependent pathologic and inflammatory consequences of various blood sampling techniques in mice","volume":"58","author":"Metzdorff","year":"2019","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1258\/002367798780599983","article-title":"Comparison of effects of restraint, cage transportation, anaesthesia and repeated bleeding on plasma glucose levels between mice and rats","volume":"32","author":"Tabata","year":"1998","journal-title":"Lab Anim."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Teilmann, A.C., Madsen, A.N., Holst, B., Hau, J., Rozell, B., and Abelson, K.S.P. (2014). Physiological and pathological impact of blood sampling by retro-bulbar sinus puncture and facial vein phlebotomy in laboratory mice. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0113225"},{"key":"ref_39","first-page":"202","article-title":"Clinical biochemistry parameters in c57bl\/6j mice after blood collection from the submandibular vein and retroorbital plexus","volume":"49","author":"Silverman","year":"2010","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_40","first-page":"711","article-title":"Comparison of submental blood collection with the retroorbital and submandibular methods in mice (mus musculus)","volume":"56","author":"Constantinescu","year":"2017","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"241","DOI":"10.7120\/09627286.24.3.241","article-title":"Extending the \u2019five domains\u2019 model for animal welfare assessment to incorporate positive welfare states","volume":"24","author":"Mellor","year":"2015","journal-title":"Anim. Welf."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1017\/S0962728600016092","article-title":"Why is there no simple way of measuring animal welfare?","volume":"2","author":"Mason","year":"1993","journal-title":"Anim. Welf."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1079\/PAVSNNR201914010","article-title":"The role of behavioural assessment in determining \u2019positive\u2019 affective states in animals","volume":"14","author":"Whittaker","year":"2019","journal-title":"CAB Rev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.physbeh.2007.02.003","article-title":"Assessment of positive emotions in animals to improve their welfare","volume":"92","author":"Boissy","year":"2007","journal-title":"Physiol. Behav."},{"key":"ref_45","unstructured":"Pickard, J. (2013). Review of the Assessment of Cumulative Severity and Lifetime Experience in Non-Human Primates Used in Neuroscience Research, Animal Procedures Committee."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Animal Health & Welfare (AHAW) (2012). Welfare. Statement on the use of animal-based measures to assess the welfare of animals. EFSA J., 10, 2767.","DOI":"10.2903\/j.efsa.2012.2767"},{"key":"ref_47","first-page":"41","article-title":"Limits to using hpa axis activity as an indication of animal welfare","volume":"32","author":"Otovic","year":"2014","journal-title":"ALTEX"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"457","DOI":"10.2527\/jas.2015-9645","article-title":"Invited review: The usefulness of measuring glucocorticoids for assessing animal welfare","volume":"94","author":"Ralph","year":"2016","journal-title":"J. Anim. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.physbeh.2018.04.033","article-title":"Measuring corticosterone concentrations over a physiological dynamic range in female rats","volume":"194","author":"Bekhbat","year":"2018","journal-title":"Physiol. Behav."},{"key":"ref_50","unstructured":"Fox, J.G., Anderson, L.C., Otto, G.M., Pritchett-Corning, K.R., and Whary, M.T. (2015). Chapter 39\u2014Animal Welfare. Laboratory Animal Medicine, Academic Press. [3rd ed.]."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.yhbeh.2003.07.002","article-title":"Analyzing corticosterone metabolites in fecal samples of mice: A noninvasive technique to monitor stress hormones","volume":"45","author":"Touma","year":"2004","journal-title":"Horm. Behav."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1111\/j.1469-185X.1982.tb00705.x","article-title":"Environmental effects on animals used in biomedical research","volume":"57","author":"Clough","year":"1982","journal-title":"Biol. Rev. Camb. Philos. Soc."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Jirkof, P., Rudeck, J., and Lewejohann, L. (2019). Assessing affective state in laboratory rodents to promote animal welfare\u2014What is the progress in applied refinement research?. Animals (Basel), 9.","DOI":"10.3390\/ani9121026"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Dietze, S., Lees, R.K., Fink, H., Brosda, J., and Voigt, J.-P. (2016). Food deprivation, body weight loss and anxiety-related behavior in rats. Animals, 6.","DOI":"10.3390\/ani6010004"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jneumeth.2014.02.001","article-title":"Burrowing and nest building behavior as indicators of well-being in mice","volume":"234","author":"Jirkof","year":"2014","journal-title":"J. Neurosci. Methods"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1038\/nprot.2007.44","article-title":"The use of the elevated plus maze as an assay of anxiety-related behavior in rodents","volume":"2","author":"Walf","year":"2007","journal-title":"Nat. Protoc."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1186\/s12915-017-0463-6","article-title":"An elevated plus-maze in mixed reality for studying human anxiety-related behavior","volume":"15","author":"Biedermann","year":"2017","journal-title":"BMC Biol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Seibenhener, M.L., and Wooten, M.C. (2015). Use of the open field maze to measure locomotor and anxiety-like behavior in mice. J. Vis. Exp., e52434.","DOI":"10.3791\/52434-v"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"79","DOI":"10.11613\/BM.2011.015","article-title":"Effects of hemolysis interference on routine biochemistry parameters","volume":"21","author":"Koseoglu","year":"2011","journal-title":"Biochem. Med. (Zagreb)"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s12959-016-0123-z","article-title":"Pre-analytical issues in the haemostasis laboratory: Guidance for the clinical laboratories","volume":"14","author":"Magnette","year":"2016","journal-title":"Thromb. J."},{"key":"ref_61","unstructured":"(1993). Removal of blood from laboratory mammals and birds: First report of the bva\/frame\/rspca\/ufaw joint working group on refinement. Lab. Anim., 27, 1\u201322."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1093\/ilar.31.4.5","article-title":"Biological effects of blood loss: Implications for sampling volumes and techniques * commentary: H. Richard Adams","volume":"31","author":"McGuill","year":"1989","journal-title":"ILAR J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"e46069","DOI":"10.15252\/embr.201846069","article-title":"Improving animal research reporting standards: Harrp, the first step of a unified approach by iclas to improve animal research reporting standards worldwide","volume":"19","author":"Osborne","year":"2018","journal-title":"EMBO Rep."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Kilkenny, C., Browne, W.J., Cuthill, I.C., Emerson, M., and Altman, D.G. (2010). Improving bioscience research reporting: The arrive guidelines for reporting animal research. PLoS Biol., 8.","DOI":"10.1371\/journal.pbio.1000412"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/j.1939-1676.2009.0441.x","article-title":"The reflect statement: Methods and processes of creating reporting guidelines for randomized controlled trials for livestock and food safety","volume":"24","author":"Sargeant","year":"2010","journal-title":"J. Vet. Intern. Med."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1111\/jvim.14574","article-title":"Methods and processes of developing the strengthening the reporting of observational studies in epidemiology\u2014Veterinary (strobe-vet) statement","volume":"30","author":"Sargeant","year":"2016","journal-title":"J. Vet. Intern. Med."},{"key":"ref_67","unstructured":"Nature Publishing Group (2020, April 30). Availability of Data & Materials. Available online: http:\/\/www.nature.com\/authors\/policies\/availability.html."},{"key":"ref_68","unstructured":"Science (2020, April 30). Science: Editorial Policies. Available online: http:\/\/www.sciencemag.org\/authors\/science-editorial-policies."},{"key":"ref_69","unstructured":"Silva, L. (2020, April 30). PLOS\u2019 New Data Policy: Public Access to Data. EveryONE: PLOS ONE Community Blog. Available online: http:\/\/blogs.plos.org\/everyone\/2014\/02\/24\/plos-new-data-policy-public-access-data-2\/."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Higgins, J.P.T., Thomas, J., Chandler, J., Cumpston, M., Li, T., Page, M.J., and Welch, V.A. (2019). Chapter 12: Synthesizing and presenting findings using other methods. Cochrane Handbook for Systematic Reviews of Interventions, Cochrane. Available online: www.training.cochrane.org\/handbook.","DOI":"10.1002\/9781119536604"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/6\/989\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:36:11Z","timestamp":1760175371000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/6\/989"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,5]]},"references-count":70,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["ani10060989"],"URL":"https:\/\/doi.org\/10.3390\/ani10060989","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,5]]}}}