{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T04:18:36Z","timestamp":1780633116862,"version":"3.54.1"},"reference-count":49,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,7,25]],"date-time":"2020-07-25T00:00:00Z","timestamp":1595635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["AGL2016-79096-R"],"award-info":[{"award-number":["AGL2016-79096-R"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003176","name":"Ministerio de Educaci\u00f3n, Cultura y Deporte","doi-asserted-by":"publisher","award":["FPU16\/02170"],"award-info":[{"award-number":["FPU16\/02170"]}],"id":[{"id":"10.13039\/501100003176","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The objective of the present study was to evaluate the possible changes of oxytocin concentrations in saliva during and after ejaculate collection in breeding boars usually used in artificial insemination programs. Saliva samples of 33 boars were collected the day before ejaculate collection (DB), during the ejaculation time (T0) and two hours after ejaculate collection (T2). Free oxytocin and oxytocin linked to proteins concentrations were measured by two methods previously developed and validated for saliva of pigs. Younger boars, boars with higher libido intensity and boars of the Pietrain breed showed higher values of oxytocin in saliva during ejaculation than the day before. In addition, boars with higher libido showed higher concentrations two hours after ejaculate collection than during the day before. These changes were of higher magnitude and significance when oxytocin linked to proteins was measured. In conclusion, this study demonstrated for the first time that ejaculation influences the salivary oxytocin concentrations in breeding boars, although this influence varies according to age, libido and breed.<\/jats:p>","DOI":"10.3390\/ani10081268","type":"journal-article","created":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T04:39:50Z","timestamp":1595824790000},"page":"1268","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Ejaculate Collection Influences the Salivary Oxytocin Concentrations in Breeding Male Pigs"],"prefix":"10.3390","volume":"10","author":[{"given":"Marina","family":"L\u00f3pez-Arjona","sequence":"first","affiliation":[{"name":"Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence \u2018Campus Mare Nostrum\u2019, University of Murcia, Campus de Espinardo s\/n, 30100 Espinardo, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Padilla","sequence":"additional","affiliation":[{"name":"Department of Medicine and Animal Surgery, Veterinary Science, University of Murcia, 30100 Murcia, Spain"},{"name":"IMIB-Arrixaca, Regional Campus of International Excellence \u2018Campus Mare Nostrum\u2019, University of Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4213-3093","authenticated-orcid":false,"given":"Jordi","family":"Roca","sequence":"additional","affiliation":[{"name":"Department of Medicine and Animal Surgery, Veterinary Science, University of Murcia, 30100 Murcia, Spain"},{"name":"IMIB-Arrixaca, Regional Campus of International Excellence \u2018Campus Mare Nostrum\u2019, University of Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Joaqu\u00edn","family":"Cer\u00f3n","sequence":"additional","affiliation":[{"name":"Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence \u2018Campus Mare Nostrum\u2019, University of Murcia, Campus de Espinardo s\/n, 30100 Espinardo, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1524-1558","authenticated-orcid":false,"given":"Silvia","family":"Mart\u00ednez-Subiela","sequence":"additional","affiliation":[{"name":"Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence \u2018Campus Mare Nostrum\u2019, University of Murcia, Campus de Espinardo s\/n, 30100 Espinardo, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1146\/annurev.pa.30.040190.002441","article-title":"Conformational and structural considerations in oxytocin-receptor binding and biological activity","volume":"30","author":"Hruby","year":"1990","journal-title":"Annu. 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