{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T10:54:28Z","timestamp":1784631268600,"version":"3.55.0"},"reference-count":86,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T00:00:00Z","timestamp":1596067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>In a cross-over design, eight rumen cannulated dairy cows were used to explore the industry-recommended method for dietary transition to fodder beet (FB: Beta vulgaris L.) on changes to rumen fermentation and pH, milk production, dry matter intake (DMI) and the risk of subacute ruminal acidosis (SARA) during early lactation. Cows were split into two groups and individually allocated a ryegrass (Lolium Perenne L.) and white clover (Trifolium repens L.) diet (HO) or the same herbage supplemented with 6 kg DM\/cow of harvested fodder beet bulbs (FBH). Dietary adaptation occurred over 20 days consisting of: stage 1: gradual transition to target FB intake (days 1\u201312, +0.5 kg DM of FB\/d); stage 2: acclimatization (days 13\u201317) and stage 3: post-adaption sampling (days 18\u201320). Response variables were analyzed as a factorial arrangement of diet and stage of adaption using a combination of ANOVA and generalized linear mixed modelling. Dietary proportion of FB represented 22, (stage 1), 32 (stage 2) and 38% (stage 3) of daily DMI. One cow during each period developed SARA from FB and the duration of low pH increased with FBH compared to the HO treatment (p &lt; 0.01). Rumen concentrations of lactic and butyric acid increased with FBH but concentrations of acetate, propionate and total volatile fatty acids (VFA) declined by 9.3% at day 20, compared to the HO treatment (p &lt; 0.01). Treatments did not affect milk production but total DMI with supplemented cows increased during the final stage of adaptation and feed conversion efficiency (FCE kg milk\/kg DM) declined with the FBH treatment. The occurrence of SARA in 25% of animals fed FB suggest it is a high-risk supplement to animal health and further evaluation of industry-recommended methods for feeding FB at the individual- and herd-scale are needed.<\/jats:p>","DOI":"10.3390\/ani10081307","type":"journal-article","created":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T12:15:38Z","timestamp":1596111338000},"page":"1307","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Supplementation of Spring Pasture with Harvested Fodder Beet Bulb Alters Rumen Fermentation and Increases Risk of Subacute Ruminal Acidosis during Early Lactation"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3220-2347","authenticated-orcid":false,"given":"Anita","family":"Fleming","sequence":"first","affiliation":[{"name":"Faculty of Agriculture and Life Sciences, PO Box 85084, Lincoln University, Lincoln 7647, Canterbury, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konagh","family":"Garrett","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture and Life Sciences, PO Box 85084, Lincoln University, Lincoln 7647, Canterbury, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Froehlich","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture and Life Sciences, PO Box 85084, Lincoln University, Lincoln 7647, Canterbury, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8571-5184","authenticated-orcid":false,"given":"Matthew","family":"Beck","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture and Life Sciences, PO Box 85084, Lincoln University, Lincoln 7647, Canterbury, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Racheal H.","family":"Bryant","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture and Life Sciences, PO Box 85084, Lincoln University, Lincoln 7647, Canterbury, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grant","family":"Edwards","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture and Life Sciences, PO Box 85084, Lincoln University, Lincoln 7647, Canterbury, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Gregorini","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture and Life Sciences, PO Box 85084, Lincoln University, Lincoln 7647, Canterbury, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/0377-8401(87)90073-3","article-title":"The chemical composition, digestibility and energy value of fodder-beet roots","volume":"18","author":"Clark","year":"1987","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_2","first-page":"261","article-title":"Effects of potassium, sodium and chloride fertiliser rates on fodder beet yield and quality in Canterbury","volume":"75","author":"Chakwizira","year":"2013","journal-title":"Proc. N. Z. Grassl. Assoc."},{"key":"ref_3","first-page":"87","article-title":"Brief Communication: Ad libitum fodder-beet and pasture beef finishing systems\u2013intake, utilisation, grazing behaviour and liveweight gains","volume":"76","author":"Saldias","year":"2016","journal-title":"Proc. N. Z. Soc. Anim. Prod."},{"key":"ref_4","first-page":"6","article-title":"Milk production and milk fatty acid composition of grazing dairy cows supplemented with fodder beet","volume":"78","author":"Fleming","year":"2018","journal-title":"N. Z. J. Anim. Sci. Prod."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1080\/00288233.2019.1675717","article-title":"A comparison of fodder beet and maize silage as supplements to pasture in late lactation to increase milk production and reduce urinary nitrogen excretion","volume":"63","author":"Dalley","year":"2019","journal-title":"N. Z. J. Agric. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1071\/AN18002","article-title":"Rumen digesta and products of fermentation in cows fed varying proportions of fodder beet (beta vulgaris L.) with fresh pasture or silage or straw","volume":"60","author":"Pacheco","year":"2020","journal-title":"Anim. Prod. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3168\/jds.S0022-0302(03)73581-4","article-title":"Invited review: Production and digestion of supplemented dairy cows on pasture","volume":"86","author":"Bargo","year":"2003","journal-title":"J. Dairy Sci."},{"key":"ref_8","first-page":"913","article-title":"Levels of pasture substitution when concentrates are fed to grazing dairy cows in northern Victoria","volume":"40","author":"Stockdale","year":"2000","journal-title":"Anim. Prod. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1080\/00288233.2017.1345762","article-title":"Range of quality characteristics of New Zealand forages and implications for reducing the nitrogen leaching risk from grazing dairy cows","volume":"60","author":"Dalley","year":"2017","journal-title":"N. Z. J. Agric. Res."},{"key":"ref_10","unstructured":"Gibbs, J. (2014). Fodder beet in the New Zealand dairy industry. The South Island Dairy Event, Stadium Southland."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1071\/AN18018","article-title":"Digestion and nitrogen excretion by Holstein\u2013Friesian cows in late lactation offered ryegrass-based pasture supplemented with fodder beet","volume":"59","author":"Waghorn","year":"2019","journal-title":"Anim. Prod. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"275","DOI":"10.2527\/1998.761275x","article-title":"Acidosis in cattle: A review","volume":"76","author":"Owens","year":"1998","journal-title":"J. Anim. Sci."},{"key":"ref_13","first-page":"406","article-title":"Subacute ruminal acidosis (SARA): A review","volume":"50","author":"Kleen","year":"2003","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.3168\/jds.S0022-0302(97)76026-0","article-title":"Bovine acidosis: Implications on laminitis","volume":"80","author":"Nocek","year":"1997","journal-title":"J. Dairy Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/1751-0147-51-39","article-title":"Ruminal acidosis and the rapid onset of ruminal parakeratosis in a mature dairy cow: A case report","volume":"51","author":"Steele","year":"2009","journal-title":"Acta Vet. Scand."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2662","DOI":"10.3168\/jds.2011-5080","article-title":"Meta-analysis reveals threshold level of rapidly fermentable dietary concentrate that triggers systemic inflammation in cattle","volume":"95","author":"Zebeli","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5067","DOI":"10.3168\/jds.2009-2102","article-title":"Repeated ruminal acidosis challenges in lactating dairy cows at high and low risk for developing acidosis: Feeding, ruminating and lying behavior","volume":"92","author":"DeVries","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3554","DOI":"10.3168\/jds.2008-1264","article-title":"Repeated Ruminal Acidosis Challenges in Lactating Dairy Cows at High and Low Risk for Developing Acidosis: Ruminal pH","volume":"91","author":"Dohme","year":"2008","journal-title":"J. Dairy Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2459","DOI":"10.3168\/jds.S0022-0302(65)88499-5","article-title":"Incidence of ruminal parakeratosis in calves fed different rations and its relation to volatile fatty acid absorption","volume":"48","author":"Bull","year":"1965","journal-title":"J. Dairy Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.3168\/jds.S0022-0302(97)76074-0","article-title":"Relationship Between Fermentation Acid Production in the Rumen and the Requirement for Physically Effective Fiber","volume":"80","author":"Allen","year":"1997","journal-title":"J. Dairy Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.2527\/jas1978.4761329x","article-title":"Relationship of rumen gram-negative bacteria and free endotoxin to lactic acidosis in cattle","volume":"47","author":"Nagaraja","year":"1978","journal-title":"J Anim Sci"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.3168\/jds.S0022-0302(05)72807-1","article-title":"Subacute ruminal acidosis induces ruminal lipopolysaccharide endotoxin release and triggers an inflammatory response","volume":"88","author":"Gozho","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/j.rvsc.2012.02.004","article-title":"Interplay between rumen digestive disorders and diet-induced inflammation in dairy cattle","volume":"93","author":"Zebeli","year":"2012","journal-title":"Res. Vet. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.tvjl.2007.12.017","article-title":"Subacute ruminal acidosis (SARA) in grazing Irish dairy cows","volume":"176","author":"Doherty","year":"2008","journal-title":"Vet. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.tvjl.2007.12.016","article-title":"Subacute ruminal acidosis in dairy cows: The physiological causes, incidence and consequences","volume":"176","author":"Plaizier","year":"2008","journal-title":"Vet. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.anifeedsci.2011.12.003","article-title":"Incidence, prevalence and impact of SARA in dairy herds","volume":"172","author":"Kleen","year":"2012","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"775","DOI":"10.2527\/jas1979.493775x","article-title":"Adaptation to high concentrate diets by beef cattle. I. Adaptation to corn and wheat diets","volume":"49","author":"Fulton","year":"1979","journal-title":"J. Anim. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s13028-015-0128-9","article-title":"Indicators of induced subacute ruminal acidosis (SARA) in Danish Holstein cows","volume":"57","author":"Danscher","year":"2015","journal-title":"Acta Vet. Scand."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2767","DOI":"10.3168\/jds.2008-1716","article-title":"Effect of dietary forage to concentrate ratio on volatile fatty acid absorption and the expression of genes related to volatile fatty acid absorption and metabolism in ruminal tissue","volume":"92","author":"Penner","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"59","DOI":"10.3168\/jds.S0022-0302(04)73142-2","article-title":"Comparison of techniques for measurement of rumen pH in lactating dairy cows","volume":"87","author":"Duffield","year":"2004","journal-title":"J. Dairy Sci"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.3168\/jds.2007-0572","article-title":"Modeling the adequacy of dietary fiber in dairy cows based on the responses of ruminal pH and milk fat production to composition of the diet","volume":"91","author":"Zebeli","year":"2008","journal-title":"J. Dairy Sci."},{"key":"ref_32","unstructured":"DairyNZ (2017). Fodder Beet Feeding Practices and Industry Issues, DairyNZ."},{"key":"ref_33","first-page":"251","article-title":"A comparison of microbial protein synthesis in beef steers fed ad libitum winter ryegrass or fodder beet","volume":"75","author":"Prendergast","year":"2015","journal-title":"Proc. N. Z. Soc. Anim. Prod."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"365","DOI":"10.3168\/jds.S0022-0302(07)72638-3","article-title":"Severity of ruminal acidosis in primiparous Holstein cows during the periparturient period","volume":"90","author":"Penner","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1080\/00480169.2018.1465484","article-title":"Feeding fodder beet (Beta vulgaris L.) with either barley straw or pasture silage to non-lactating dairy cows","volume":"66","author":"Waghorn","year":"2018","journal-title":"N. Z. Vet. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.anifeedsci.2011.12.005","article-title":"Ruminal pH regulation and nutritional consequences of low pH","volume":"172","author":"Dijkstra","year":"2012","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1080\/1828051X.2016.1141330","article-title":"Changes in milk production and milk fatty acid composition of cows switched from pasture to a total mixed ration diet and back to pasture","volume":"15","author":"Rego","year":"2016","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Senn, S. (2002). Cross-Over Trials in Clinical Research, John Wiley & Sons.","DOI":"10.1002\/0470854596"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"AOAC (2003). Crude fat, diethyl ether extraction, in feed, cereal grain, and forage (2003.05). J. AOAC Int., 86, 888\u2013898.","DOI":"10.1093\/jaoac\/86.5.888"},{"key":"ref_40","unstructured":"CSIRO (2007). Nutrient Requirements of Domesticated Ruminants, CSIRO Publishing."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0021-9673(97)01007-8","article-title":"Fully automated precolumn derivatization, on-line dialysis and high-performance liquid chromatographic analysis of amino acids in food, beverages and feedstuff","volume":"798","author":"Heems","year":"1998","journal-title":"J. Chromatogr. A"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v067.i01","article-title":"Fitting linear mixed-effects models using lme4","volume":"67","author":"Bates","year":"2015","journal-title":"J. Stat. Softw."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"378","DOI":"10.32614\/RJ-2017-066","article-title":"glmmTMB balances speed and flexibility among packages for zero-inflated generalized linear mixed modeling","volume":"9","author":"Brooks","year":"2017","journal-title":"R J."},{"key":"ref_44","unstructured":"Lenth, R.V. (2020, June 26). Using Lsmeans. Available online: https:\/\/cran.r-project.org\/web\/packages\/lsmeans\/lsmeans.pdf."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"570","DOI":"10.3168\/jds.S0022-0302(02)74110-6","article-title":"Effect of subacute ruminal acidosis on in situ fiber digestion in lactating dairy cows","volume":"85","author":"Plaizier","year":"2002","journal-title":"J. Dairy Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4404","DOI":"10.3168\/jds.S0022-0302(06)72487-0","article-title":"Rumen Lipopolysaccharide and Inflammation During Grain Adaptation and Subacute Ruminal Acidosis in Steers","volume":"89","author":"Gozho","year":"2006","journal-title":"J. Dairy Sci."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Aquilani, R., Maestri, R., Boselli, M., Achilli, M.P., Arrigoni, N., Bruni, M., and Barbieri, A. (2019). The relationship between plasma amino acids and circulating albumin and haemoglobin in postabsorptive stroke patients. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0219756"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.3168\/jds.2008-1389","article-title":"A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation","volume":"92","author":"Khafipour","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/2049-1891-4-31","article-title":"Changes in feed intake, nutrient digestion, plasma metabolites, and oxidative stress parameters in dairy cows with subacute ruminal acidosis and its regulation with pelleted beet pulp","volume":"4","author":"Guo","year":"2013","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.3168\/jds.S0022-0302(97)76075-2","article-title":"Creating a system for meeting the fiber requirements of dairy cows","volume":"80","author":"Mertens","year":"1997","journal-title":"J. Dairy Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.3168\/jds.S0022-0302(86)80534-3","article-title":"Eating and resting salivation in early lactation dairy cows","volume":"69","author":"Cassida","year":"1996","journal-title":"J. Dairy Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.tvjl.2015.02.005","article-title":"The effects on ruminal pH and serum haptoglobin after feeding a grain-based supplement to grazing dairy cows as a partial mixed ration or during milking","volume":"204","author":"Coombe","year":"2015","journal-title":"Vet. J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3052","DOI":"10.2527\/1996.74123052x","article-title":"Metabolic constraints on voluntary intake in ruminants","volume":"74","author":"Illius","year":"1996","journal-title":"J. Anim. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.2527\/2000.78123155x","article-title":"Evaluation of models of acute and subacute acidosis on dry matter intake, ruminal fermentation, blood chemistry, and endocrine profiles of beef steers","volume":"78","author":"Brown","year":"2000","journal-title":"J. Anim. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3938","DOI":"10.3168\/jds.2014-8449","article-title":"Feed conversion efficiency in dairy cows: Repeatability, variation in digestion and metabolism of energy and nitrogen, and ruminal methanogens","volume":"98","author":"Arndt","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3105","DOI":"10.3168\/jds.S0022-0302(04)73446-3","article-title":"Invited Review: Pathology, Etiology, Prevention and Treatment of Fatty Liver in Dairy Cows","volume":"87","author":"Bobe","year":"2004","journal-title":"J. Dairy Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1017\/S0022029911000550","article-title":"Milk fatty acid profile related to energy balance in dairy cows","volume":"78","author":"Gross","year":"2011","journal-title":"J. Dairy Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1051\/animres:2000117","article-title":"Ruminant milk fat plasticity: Nutritional control of saturated, polyunsaturated, trans and conjugated fatty acids","volume":"49","author":"Chilliard","year":"2000","journal-title":"Ann. Zootech."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.3168\/jds.S0022-0302(82)82367-9","article-title":"Feed intake, apparent diet digestibility, and rate of particulate passage in dairy cattle","volume":"65","author":"Colucci","year":"1982","journal-title":"J. Dairy Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1071\/AN10170","article-title":"Feed conversion efficiency and marginal milk production responses of pasture-fed dairy cows offered supplementary grain during an extended lactation","volume":"51","author":"Auldist","year":"2011","journal-title":"Anim. Prod. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.3168\/jds.2011-4506","article-title":"Effect of feed restriction on metabolites in cerebrospinal fluid and plasma of dairy cows","volume":"95","author":"Laeger","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1042\/bj1281311","article-title":"Effects of starvation on intermediary metabolism in the lactating cow. A comparison with metabolic changes occurring during bovine ketosis","volume":"128","author":"Baird","year":"1972","journal-title":"Biochem. J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1079\/BJN19930041","article-title":"Absorption of volatile fatty acids from the rumen of lactating dairy cows as influenced by volatile fatty acid concentration, pH and rumen liquid volume","volume":"69","author":"Dijkstra","year":"1993","journal-title":"Br. J. Nutr."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"3055","DOI":"10.2527\/1991.6973055x","article-title":"Effect of coliform bacteria, feed deprivation, and pH on ruminal D-lactic acid production by steer or continuous-culture microbial populations changed from forage to concentrates","volume":"69","author":"Slyter","year":"1991","journal-title":"J. Anim. Sci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"E17","DOI":"10.3168\/jds.2006-478","article-title":"Ruminal acidosis in beef cattle: The current microbiological and nutritional outlook","volume":"90","author":"Nagaraja","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.2527\/1996.7471694x","article-title":"Propionate Modulation of Ruminal Ketogenesis","volume":"74","author":"Baldwin","year":"1996","journal-title":"J. Anim. Sci."},{"key":"ref_67","first-page":"37","article-title":"Hydrogen production and transfer in the rumen","volume":"12","author":"Hegarty","year":"1999","journal-title":"Recent Adv. Anim. Nutr. Aust."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"3980","DOI":"10.3168\/jds.2010-4091","article-title":"Effects of ruminal ammonia and butyrate concentrations on reticuloruminal epithelial blood flow and volatile fatty acid absorption kinetics under washed reticulorumen conditions in lactating dairy cows","volume":"94","author":"Storm","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"5866","DOI":"10.3168\/jds.2011-5167","article-title":"The relationship between rumen acidosis resistance and expression of genes involved in regulation of intracellular pH and butyrate metabolism of ruminal epithelial cells in steers","volume":"95","author":"Schlau","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_70","unstructured":"Penner, G.B. (2014, January 4). Mechanisms of volatile fatty acid absorption and metabolism and maintenance of a stable rumen environment. Proceedings of the 25th Florida Ruminant Nutrition Symposium, Gainsville, FL, USA."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2919","DOI":"10.3168\/jds.2011-4239","article-title":"A model of ruminal volatile fatty acid absorption kinetics and rumen epithelial blood flow in lactating Holstein cows","volume":"95","author":"Storm","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.3168\/jds.S0022-0302(78)83694-7","article-title":"Rumen epithelial cell proliferation accelerated by rapid increase in intraruminal butyrate","volume":"61","author":"Sakata","year":"1998","journal-title":"J. Dairy Sci."},{"key":"ref_73","first-page":"501","article-title":"The effect of diet, intraruminal pH and osmolarity on sodium, chloride and magnesium absorption from the temporarily isolated and washed reticulo-rumen of sheep","volume":"72","author":"Martens","year":"1987","journal-title":"J. Exp. Physiol. Transl. Integr."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1079\/PNS19760038","article-title":"The effect of processing on digestion and utilization of cereals by ruminants","volume":"35","year":"1976","journal-title":"Proc. Nutr. Soc."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1152\/ajplegacy.1967.212.4.747","article-title":"Insulin release by acetate, propionate, butyrate, and glucose in lambs and adult sheep","volume":"212","author":"Manns","year":"1964","journal-title":"Am. J. Physiol. Leg. Content"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1113\/jphysiol.1964.sp007522","article-title":"The oxidation and utilization of glucose and acetate by the mammary gland of the goat in relation to their over-all metabolism and to milk formation","volume":"175","author":"Annison","year":"1964","journal-title":"J. Physiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1093\/jn\/128.2.293S","article-title":"Use of isolated ruminal epithelial cells in the study of rumen metabolism","volume":"128","author":"Baldwin","year":"1998","journal-title":"J. Nutr."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s40104-019-0347-4","article-title":"Prepartum body conditions affect insulin signaling pathways in postpartum adipose tissues in transition dairy cows","volume":"10","author":"Zhang","year":"2019","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_79","unstructured":"Aschenbach, J.R., Penner, G.B., Stumpff, F., and G\u00e4bel, G. (2010, January 10\u201315). Role of fermentation acid absorption in the regulation of ruminal pH. Proceedings of the Ruminant Nutrition Symposium, Denver, CO, USA."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.3168\/jds.2015-9837","article-title":"Morphological adaptation of rumen papillae during the dry period and early lactation as affected by rate of increase of concentrate allowance","volume":"99","author":"Dieho","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"116","DOI":"10.21423\/bovine-vol1985no20p116-120","article-title":"Adaptive changes of the ruminal mucosa and their functional and clinical significance","volume":"20","author":"Dirksen","year":"1985","journal-title":"Bov. Pract."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1080\/17450390802506885","article-title":"Change of ruminal sodium transport in sheep during dietary adaptation","volume":"63","author":"Etschmann","year":"2009","journal-title":"Arch. Anim. Nutr."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"8733","DOI":"10.3168\/jds.2016-11570","article-title":"Characteristics of dairy cows with a greater or lower risk of subacute ruminal acidosis: Volatile fatty acid absorption, rumen digestion and expression of genes in rumen epithelial cells","volume":"99","author":"Gao","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"3006","DOI":"10.3168\/jds.2013-7472","article-title":"Relationship of severity of subacute ruminal acidosis to rumen fermentation, chewing activities, sorting behavior, and milk production in lactating dairy cows fed a high-grain diet","volume":"97","author":"Gao","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1071\/EA05349","article-title":"Feeding concentrates based on individual cow requirements improves the yield of milk solids in dairy cows grazing restricted pasture","volume":"47","author":"Pedernera","year":"2007","journal-title":"J. Exp. Agric."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"3116","DOI":"10.3168\/jds.2008-1718","article-title":"Competition at the feed bunk changes the feeding, standing, and social behavior of transition dairy cows","volume":"92","author":"Proudfoot","year":"2009","journal-title":"J. Dairy Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/8\/1307\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:52:55Z","timestamp":1760176375000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/8\/1307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,30]]},"references-count":86,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2020,8]]}},"alternative-id":["ani10081307"],"URL":"https:\/\/doi.org\/10.3390\/ani10081307","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,30]]}}}