{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:03:06Z","timestamp":1791950586915,"version":"4.3.4"},"reference-count":64,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T00:00:00Z","timestamp":1596672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["REPBIOTECH675526"],"award-info":[{"award-number":["REPBIOTECH675526"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2015-66341-R"],"award-info":[{"award-number":["AGL2015-66341-R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2015-70140-R"],"award-info":[{"award-number":["AGL2015-70140-R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n S\u00e9neca","doi-asserted-by":"publisher","award":["20040\/GERM\/16"],"award-info":[{"award-number":["20040\/GERM\/16"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The increasing use of in vitro embryo production (IVP) followed by embryo transfer (ET), alongside with cryopreservation of embryos, has risen concerns regarding the possible altered pregnancy rates, calving or even neonatal mortality. One of the hypotheses for these alterations is the current culture conditions of the IVP. In an attempt to better mimic the physiological milieu, embryos were produced with female reproductive fluids (RF) as supplements to culture medium, and another group of embryos were supplemented with bovine serum albumin (BSA) as in vitro control. Embryos were cryopreserved and transferred while, in parallel, an in vivo control (artificial insemination, AI) with the same bull used for IVP was included. An overview on pregnancy rates, recipients\u2019 hormonal levels, parturition, and resulting calves were recorded. Results show much similarity between groups in terms of pregnancy rates, gestation length and calves\u2019 weight. Nonetheless, several differences on hormonal levels were noted between recipients carrying AI embryos especially when compared to BSA. Some calving issues and neonatal mortality were observed in both IVP groups. In conclusion, most of the parameters studied were similar between both types of IVP derived embryos and the in vivo-derived embryos, suggesting that the IVP technology used was efficient enough for the safe production of calves.<\/jats:p>","DOI":"10.3390\/ani10081359","type":"journal-article","created":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T09:41:21Z","timestamp":1596706881000},"page":"1359","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Reproductive Outcomes and Endocrine Profile in Artificially Inseminated versus Embryo Transferred Cows"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8419-7845","authenticated-orcid":false,"given":"Jordana S.","family":"Lopes","sequence":"first","affiliation":[{"name":"Physiology of Reproduction Group, Departamento de Fisiolog\u00eda, Facultad de Veterinaria, Universidad de Murcia, Campus Mare Nostrum, 30100 Murcia, Spain"},{"name":"Institute for Biomedical Research of Murcia, IMIB-Arrixaca, 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estefan\u00eda","family":"Alc\u00e1zar-Trivi\u00f1o","sequence":"additional","affiliation":[{"name":"El Barranquillo S.L., Torre Pacheco, 30700 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2880-3342","authenticated-orcid":false,"given":"Cristina","family":"Soriano-\u00dabeda","sequence":"additional","affiliation":[{"name":"Physiology of Reproduction Group, Departamento de Fisiolog\u00eda, Facultad de Veterinaria, Universidad de Murcia, Campus Mare Nostrum, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meriem","family":"Hamdi","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, National Institute for Agriculture and Food Research and Technology (INIA), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4190-6258","authenticated-orcid":false,"given":"Sebastian","family":"C\u00e1novas","sequence":"additional","affiliation":[{"name":"Physiology of Reproduction Group, Departamento de Fisiolog\u00eda, Facultad de Veterinaria, Universidad de Murcia, Campus Mare Nostrum, 30100 Murcia, Spain"},{"name":"Institute for Biomedical Research of Murcia, IMIB-Arrixaca, 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6813-3940","authenticated-orcid":false,"given":"Dimitrios","family":"Rizos","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, National Institute for Agriculture and Food Research and Technology (INIA), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3943-1890","authenticated-orcid":false,"given":"Pilar","family":"Coy","sequence":"additional","affiliation":[{"name":"Physiology of Reproduction Group, Departamento de Fisiolog\u00eda, Facultad de Veterinaria, Universidad de Murcia, Campus Mare Nostrum, 30100 Murcia, Spain"},{"name":"Institute for Biomedical Research of Murcia, IMIB-Arrixaca, 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,6]]},"reference":[{"key":"ref_1","first-page":"7","article-title":"IETS Data Retrieval Committee. 2018 statistics of embryo production and transfer in domestic farm animals","volume":"37","author":"Viana","year":"2019","journal-title":"Embryo Technol. 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