{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T15:01:12Z","timestamp":1780066872336,"version":"3.54.0"},"reference-count":52,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T00:00:00Z","timestamp":1596758400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["033L046"],"award-info":[{"award-number":["033L046"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Cryopreservation is important for animal fertility and biodiversity. Unfortunately, cryopreservation of feline oocytes is still an experimental technique. The aims of this study were to analyze the potential toxicity of the cryoprotectants in the vitrification solution (VS) on cat oocytes and to investigate whether the meiotic status of oocytes influences their developmental potential after vitrification. Two experiments were conducted with the VS composed of 20% ethylene glycol, 20% dimethyl sulfoxide, 20% fetal calf serum, 1.5 M trehalose, and 10% Ficoll PM-70: (1) toxicity assessment of the VS on immature cumulus oocyte complexes (COCs), and subsequently in vitro maturation (IVM) and in vitro fertilization; (2) assessment of the influence of the meiotic status on vitrification effectiveness, where immature and in vitro matured COCs were vitrified on the Cryotop. After rewarming, vitrified oocytes were subjected to IVM (immature) and intracytoplasmic sperm injection (ICSI) with fresh epididymal sperm. The toxicity test revealed no negative effect of oocyte exposure to the applied VS on their developmental potential (p &gt; 0.05). Although the vitrification procedure itself significantly reduced the meiotic competence of oocytes, their meiotic status before vitrification (immature vs. in vitro matured) did not influence fertilization and morula rates. The only parameter affected by vitrification was the rate of oocytes suitable for ICSI, which was significantly lower for immature oocytes. Regardless of the meiotic status of vitrified oocytes, morphologically normal morulae were obtained. Moreover, the two meiotic stages examined are suitable for vitrification, with mature oocytes being a better choice when a well-equipped laboratory is available.<\/jats:p>","DOI":"10.3390\/ani10081371","type":"journal-article","created":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T07:25:03Z","timestamp":1597044303000},"page":"1371","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Meiotic Status Does Not Affect the Vitrification Effectiveness of Domestic Cat Oocytes"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6576-8972","authenticated-orcid":false,"given":"Natalia","family":"Sowi\u0144ska","sequence":"first","affiliation":[{"name":"Department of Reproduction, Poznan University of Life Science, ul. Wo\u0142y\u0144ska 35, 60-637 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Zahmel","sequence":"additional","affiliation":[{"name":"Department of Reproduction Biology, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Stra\u00dfe 17, 10315 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wojciech","family":"Ni\u017ca\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Clinic of Farm Animals, Wroclaw University of Environmental and Life Science, Pl. Grunwaldzki 49, 50-366 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romy","family":"Hribal","sequence":"additional","affiliation":[{"name":"Department of Reproduction Biology, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Stra\u00dfe 17, 10315 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Fernandez-Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Reproduction Biology, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Stra\u00dfe 17, 10315 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarina","family":"Jewgenow","sequence":"additional","affiliation":[{"name":"Department of Reproduction Biology, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Stra\u00dfe 17, 10315 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1071\/RD10192","article-title":"Prolonged storage of epididymal spermatozoa does not affect their capacity to fertilise in vitro matured domestic cat (Felis catus) oocytes when using ICSI","volume":"23","author":"Ringleb","year":"2011","journal-title":"Reprod. 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