{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T04:33:29Z","timestamp":1779338009766,"version":"3.51.4"},"reference-count":20,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T00:00:00Z","timestamp":1600646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"publisher","award":["20180430"],"award-info":[{"award-number":["20180430"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The consumption of fish and shellfish worldwide is steadily increasing, and tuna is a particularly valuable fish species. However, infection caused by Kudoa spp. is causing problems in many fish including the Pacific bluefin tuna (Thunnus orientalis), and there is much controversy about the association of these infections with foodborne disease. In this study, using haematological and histological analyses of the blood and internal organs (liver, spleen, kidney, heart, stomach, intestine, gill, and muscle) of Pacific bluefin tuna cultured in South Korea, infection with Myxosporea was first identified, and molecular biological analysis was conducted. In this study, Kudoa hexapunctata was finally identified. The Pacific bluefin tunas analysed in this study did not show any gross pathology lesions, such as visible cysts and\/or myoliquefaction, of infection with this species. The histological analytical results can provide guidelines for the identification of K. hexapunctata. In the case of K. hexapunctata-induced infection, unlike other countries, such as Japan, there have been no reports in South Korea, and this study is the first to detect K. hexapunctata infection in Pacific bluefin tuna cultured in South Korea. The correlation between K. hexapunctata and food poisoning is not yet clear, however, it is thought that continuous observation of its infection is necessary.<\/jats:p>","DOI":"10.3390\/ani10091705","type":"journal-article","created":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T08:18:01Z","timestamp":1600676281000},"page":"1705","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The First Detection of Kudoa hexapunctata in Farmed Pacific Bluefin Tuna in South Korea, Thunnus orientalis (Temminck and Schlegel, 1844)"],"prefix":"10.3390","volume":"10","author":[{"given":"Gyoungsik","family":"Kang","sequence":"first","affiliation":[{"name":"Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, Tongyeong 53064, Gyeongsangnam-do, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwang-Min","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, Tongyeong 53064, Gyeongsangnam-do, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-Hee","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, Tongyeong 53064, Gyeongsangnam-do, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Soo","family":"Joo","sequence":"additional","affiliation":[{"name":"Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, Tongyeong 53064, Gyeongsangnam-do, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Jin","family":"Heo","sequence":"additional","affiliation":[{"name":"Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, Tongyeong 53064, Gyeongsangnam-do, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5958-1044","authenticated-orcid":false,"given":"Won-Sik","family":"Woo","sequence":"additional","affiliation":[{"name":"Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, Tongyeong 53064, Gyeongsangnam-do, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chan-Il","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, Tongyeong 53064, Gyeongsangnam-do, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Shamsi, S. (2019). Seafood-Borne Parasitic Diseases: A \u201cOne-Health\u201d Approach Is Needed. Fishes, 4.","DOI":"10.3390\/fishes4010009"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"151","DOI":"10.4315\/0362-028X.JFP-18-258","article-title":"Survey and Exposure Assessment of Biogenic Amines in Fish Species Commonly Consumed in Korea","volume":"82","author":"Kang","year":"2019","journal-title":"J. Food Prot."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3549","DOI":"10.1111\/are.14310","article-title":"Growth and survival model of Pacific bluefin tuna (Thunnus orientalis) for capture-based aquaculture in Mexico","volume":"50","author":"Pardo","year":"2019","journal-title":"Aquac. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.ijfoodmicro.2012.03.018","article-title":"Development of a quantitative polymerase chain reaction assay for detection of Kudoa septempunctata in olive flounder (Paralichthys olivaceus)","volume":"156","author":"Harada","year":"2012","journal-title":"Int. J. Food Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1093\/cid\/cir1040","article-title":"Identification of Kudoa septempunctata as the Causative Agent of Novel Food Poisoning Outbreakes in Japan by Consumption of Paralichthys olivaceus in Raw Fish","volume":"54","author":"Kawai","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"102048","DOI":"10.1016\/j.parint.2019.102048","article-title":"Detection of Kudoa hexapunctata and Kudoa neothunni from retail raw tuna in Japan using a novel duplex polymerase chain reaction","volume":"75","author":"Arai","year":"2020","journal-title":"Parasitol. Int."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2014.11.001","article-title":"Detection rate of diarrhea-causing Kudoa hexapunctata in Pacific bluefin tuna Thunnus orientalis from Japanese waters","volume":"194","author":"Suzuki","year":"2015","journal-title":"Int. J. Food Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1007\/s00436-016-5369-7","article-title":"Incidence of three Kudoa spp., K. neothunni, K. hexapunctata, and K. thunni (Myxosporea: Multivalvulida), in Thunnus tunas distributed in the western Pacific Ocean","volume":"116","author":"Kasai","year":"2017","journal-title":"Parasitol. Res."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Ohnishi, T., Kikuchi, Y., Furusawa, H., Kamata, Y., and Sugita-Konishi, Y. (2013). Kudoa septempunctata Invasion Increases the Permeability of Human Intestinal Epithelial Monolayer. Foodborne Pathog. Dis., 10.","DOI":"10.1089\/fpd.2012.1294"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.parint.2014.03.006","article-title":"Kudoa hexapunctata n. sp. (Myxozoa: Multivalvulida) from the somatic muscle of Pacific bluefin tuna Thunnus orientalis and re-description of K. neothunni in yellowfin tuna T. albacares","volume":"63","author":"Yokoyama","year":"2014","journal-title":"Parasitol. Int."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"468","DOI":"10.11626\/KJEB.2017.35.4.468","article-title":"A Study about Analysis Results for Kudoa septempunctata (Myxosporea: Multivalvulida) in Tissue at Olive Flounder, using PCR (polymerase chain reaction) and Histological Methods","volume":"35","author":"Do","year":"2017","journal-title":"Korean J. Environ. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"79","DOI":"10.3147\/jsfp.39.79","article-title":"Kudoa thyrsites from Japanese Flounder and Kudoa lateolabracis n. sp. from Chinese Sea Bass: Causative Myxozoans of Post-Mortem Myoliquefaction","volume":"39","author":"Yokoyama","year":"2004","journal-title":"Fish Pathol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1007\/s00436-010-1941-8","article-title":"Kudoa septempunctata n. sp. (Myxosporea: Multivalvulida) from an aquacultured olive flounder (Paralichthys olivaceus) imported from Korea","volume":"107","author":"Matsukane","year":"2010","journal-title":"Parasitol. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"141","DOI":"10.14252\/foodsafetyfscj.2014026","article-title":"Novel Foodborne Disease Associated with Consumption of Raw Fish, Olive Flounder (Paralichthys olivaceus)","volume":"2","author":"Sato","year":"2014","journal-title":"Food Saf."},{"key":"ref_15","unstructured":"Barnham, C., and Baxter, A. (1998). Fisheries Notes: Condition Factor, K, for Salmonid Fish, Department of Primary Industries."},{"key":"ref_16","first-page":"281","article-title":"Determination of suitable protein and lipid levels in diets for Pacific bluefin tuna, Thunnus orientalis at grow-out stage","volume":"64","author":"Biswas","year":"2016","journal-title":"Aquac. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"262","DOI":"10.2307\/3284453","article-title":"Phylogenetic Relationships among Eight Eimeria Species Infecting Domestic Fowl Inferred Using Complete Small Subunit Ribosomal DNA Sequences","volume":"83","author":"Barta","year":"1997","journal-title":"J. Parasitol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1016\/j.ijpara.2006.06.016","article-title":"The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis","volume":"36","author":"Fiala","year":"2016","journal-title":"Int. J. Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.ympev.2009.05.018","article-title":"Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide an effective tool for their molecular phylogenetics","volume":"53","author":"Bartosova","year":"2009","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.aquaculture.2012.01.022","article-title":"Diagnostic PCR assays to detect and differentiate Kudoa septempunctata, K. thyrsites and K. lateolabracis (Myxozoa, Multivalvulida) in muscle tissue of olive flounder (Paralichthys olivaceus)","volume":"338","author":"Grabner","year":"2012","journal-title":"Aquaculture"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/9\/1705\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:11:54Z","timestamp":1760177514000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/9\/1705"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,21]]},"references-count":20,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,9]]}},"alternative-id":["ani10091705"],"URL":"https:\/\/doi.org\/10.3390\/ani10091705","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,21]]}}}