{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T19:30:04Z","timestamp":1792697404035,"version":"4.5.0"},"reference-count":33,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,9,22]],"date-time":"2020-09-22T00:00:00Z","timestamp":1600732800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31672571"],"award-info":[{"award-number":["31672571"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Streptococcus agalactiae (GBS) is a highly contagious pathogen which not only can cause neonatal meningitis, pneumonia, and septicemia but is also considered to be a major cause of bovine mastitis (BM), leading to large economic losses to the dairy industry worldwide. Like many other pathogenic bacteria, GBS also has the capacity to form a biofilm structure in the host to cause persistent infection. Tea saponin (TS), is one of the main active agents extracted from tea ash powder, and it has good antioxidant and antibacterial activities. In this study, we confirmed that TS has a slight antibacterial activity against a Streptococcus agalactiae strain isolated from dairy cow with mastitis and inhibits its biofilm formation. By performing scanning electron microscopy (SEM) experiments, we observed that with addition of TS, the biofilm formed by this GBS strain exhibited looser structure and lower density. In addition, the results of real-time reverse transcription polymerase chain reaction (RT-PCR) experiments showed that TS inhibited biofilm formation by down-regulating the transcription of the biofilm-associated genes including srtA, fbsC, neuA, and cpsE.<\/jats:p>","DOI":"10.3390\/ani10091713","type":"journal-article","created":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T02:56:43Z","timestamp":1600916203000},"page":"1713","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Anti-Biofilm Effect of Tea Saponin on a Streptococcus agalactiae Strain Isolated from Bovine Mastitis"],"prefix":"10.3390","volume":"10","author":[{"given":"Fei","family":"Shang","sequence":"first","affiliation":[{"name":"School of Life Sciences, Anhui Agricultural University, Hefei 230036, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Anhui Agricultural University, Hefei 230036, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5644-6613","authenticated-orcid":false,"given":"Ting","family":"Xue","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Anhui Agricultural University, Hefei 230036, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.tvjl.2005.01.006","article-title":"Biofilms: A role in recurrent mastitis infections?","volume":"171","author":"Melchior","year":"2006","journal-title":"Vet. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1053\/tvjl.2002.0724","article-title":"Bovine mastitis: An evolving disease","volume":"164","author":"Bradley","year":"2002","journal-title":"Vet. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1080\/01652176.2007.9695224","article-title":"Economic effects of bovine mastitis and mastitis management: A review","volume":"29","author":"Halasa","year":"2007","journal-title":"Vet. Q."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.micpath.2019.03.035","article-title":"Antimicrobial resistance and virulence genes of Streptococcus isolated from dairy cows with mastitis in China","volume":"131","author":"Tian","year":"2019","journal-title":"Microb. Pathog."},{"key":"ref_5","first-page":"190","article-title":"Occurrence, forms and etiologic agents of mastitis in Poland depending on size of farm","volume":"67","author":"Smulski","year":"2011","journal-title":"Med. Weter."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2724","DOI":"10.3168\/jds.2017-13554","article-title":"Symposium review: Novel strategies to genetically improve mastitis resistance in dairy cattle","volume":"101","author":"Martin","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1038\/nrmicro2415","article-title":"The biofilm matrix","volume":"8","author":"Flemming","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/S0966-842X(00)01913-2","article-title":"Mechanisms of biofilm resistance to antimicrobial agents","volume":"9","author":"Mah","year":"2001","journal-title":"Trends Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1099\/00221287-147-1-3","article-title":"Biofilm exopolysaccharides: A strong and sticky framework","volume":"147","author":"Sutherland","year":"2001","journal-title":"Microbiology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3389\/fcimb.2015.00006","article-title":"Biofilm formation by Streptococcus agalactiae: Influence of environmental conditions and implicated virulence factors","volume":"5","author":"Rosini","year":"2015","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Kaczorek, E., Malaczewska, J., Wojcik, R., and Siwicki, A.K. (2017). Biofilm production and other virulence factors in Streptococcus spp. isolated from clinical cases of bovine mastitis in Poland. BMC Vet. Res., 13.","DOI":"10.1186\/s12917-017-1322-y"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Rinaudo, C.D., Rosini, R., Galeotti, C.L., Berti, F., Necchi, F., Reguzzi, V., Ghezzo, C., Telford, J.L., Grandi, G., and Maione, D. (2010). Specific involvement of pilus type 2a in biofilm formation in group B Streptococcus. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0009216"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Beyene, T., Hayishe, H., Gizaw, F., Beyi, A.F., Abunna, F., Mammo, B., Ayana, D., Waktole, H., and Abdi, R.D. (2017). Prevalence and antimicrobial resistance profile of Staphylococcus in dairy farms, abattoir and humans in Addis Ababa, Ethiopia. BMC Res. Notes, 10.","DOI":"10.1186\/s13104-017-2487-y"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1751-0147-53-4","article-title":"Udder pathogens and their resistance to antimicrobial agents in dairy cows in Estonia","volume":"53","author":"Kalmus","year":"2011","journal-title":"Acta Vet. Scand"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"517","DOI":"10.14202\/vetworld.2017.517-520","article-title":"Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal","volume":"10","author":"Das","year":"2017","journal-title":"Vet. World"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3389\/fvets.2017.00063","article-title":"Whole-Genome Sequence Analysis of Antimicrobial Resistance Genes in Streptococcus uberis and Streptococcus dysgalactiae Isolates from Canadian Dairy Herds","volume":"4","author":"Velez","year":"2017","journal-title":"Front. Vet. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/rda.13032","article-title":"Mastitis treatment-Reduction in antibiotic usage in dairy cows","volume":"5","author":"Kromker","year":"2017","journal-title":"Reprod. Domest. Anim."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Cao, Q., Ma, K., Nie, M., Dong, Y., and Liu, Y. (2020). Role of luxS in immune evasion and pathogenicity of piscine Streptococcus agalactiae is not dependent on autoinducer-2. Fish Shellfish Immunol., 99.","DOI":"10.1016\/j.fsi.2020.02.016"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.micpath.2017.12.024","article-title":"Bovine mastitis: An appraisal of its alternative herbal cure","volume":"114","author":"Mushtaq","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1248\/yakushi1947.116.3_238","article-title":"Antimicrobial and anti-inflammatory actions of tea-leaf saponin","volume":"116","author":"Sagesaka","year":"1996","journal-title":"Yakugaku Zasshi"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1002\/ptr.696","article-title":"Antiinflammatory and antioxidant property of saponins of tea (Camellia sinensis (L) O. Kuntze) root extract","volume":"15","author":"Sur","year":"2001","journal-title":"Phytother. Res. PTR"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3486106","DOI":"10.1155\/2018\/3486106","article-title":"Green Tea Seed Isolated Saponins Exerts Antibacterial Effects against Various Strains of Gram Positive and Gram Negative Bacteria, a Comprehensive Study In Vitro and In Vivo","volume":"2018","author":"Khan","year":"2018","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6032","DOI":"10.1128\/IAI.72.10.6032-6039.2004","article-title":"Enterococcal surface protein, Esp, enhances biofilm formation by Enterococcus faecalis","volume":"72","author":"Tendolkar","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"10047","DOI":"10.1021\/acsami.7b02380","article-title":"Transcriptome Analysis Reveals Silver Nanoparticle-Decorated Quercetin Antibacterial Molecular Mechanism","volume":"9","author":"Sun","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3975","DOI":"10.1128\/IAI.00699-12","article-title":"CsrRS and environmental pH regulate group B streptococcus adherence to human epithelial cells and extracellular matrix","volume":"80","author":"Park","year":"2012","journal-title":"Infect. Immun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.3389\/fmicb.2019.01447","article-title":"Genomic Insights Into the Distribution and Evolution of Group B Streptococcus","volume":"10","author":"Chen","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1007\/s11250-012-0320-0","article-title":"Prevalence of subclinical mastitis and isolated udder pathogens in dairy cows in Southern Vietnam","volume":"45","author":"Ostensson","year":"2013","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"8486","DOI":"10.3168\/jds.2015-9899","article-title":"Ethanol extract of Sanguisorba officinalis L. inhibits biofilm formation of methicillin-resistant Staphylococcus aureus in an ica-dependent manner","volume":"98","author":"Chen","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_29","first-page":"269","article-title":"Biofilm formation, hemolysin production and antimicrobial susceptibilities of Streptococcus agalactiae isolated from the mastitis milk of dairy cows in Shahrekord district, Iran","volume":"4","author":"Ebrahimi","year":"2013","journal-title":"Vet. Res. Forum Int. Q. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1007\/s11250-011-9960-8","article-title":"Effects of tea saponins on rumen microbiota, rumen fermentation, methane production and growth performance--a review","volume":"44","author":"Wang","year":"2012","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1111\/j.1472-765X.2008.02459.x","article-title":"Effect of tea saponin on methanogenesis, microbial community structure and expression of mcrA gene, in cultures of rumen micro-organisms","volume":"47","author":"Guo","year":"2008","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Liu, Y., Ma, T., Chen, D., Zhang, N., Si, B., Deng, K., Tu, Y., and Diao, Q. (2019). Effects of Tea Saponin Supplementation on Nutrient Digestibility, Methanogenesis, and Ruminal Microbial Flora in Dorper Crossbred Ewe. Animals, 9.","DOI":"10.3390\/ani9010029"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"8043","DOI":"10.3168\/jds.2016-12425","article-title":"Effect of tea saponins on milk performance, milk fatty acids, and immune function in dairy cow","volume":"100","author":"Wang","year":"2017","journal-title":"J. Dairy Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/9\/1713\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:12:18Z","timestamp":1760177538000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/9\/1713"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,22]]},"references-count":33,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,9]]}},"alternative-id":["ani10091713"],"URL":"https:\/\/doi.org\/10.3390\/ani10091713","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,22]]}}}