{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T08:04:34Z","timestamp":1787990674428,"version":"build-2784847793"},"reference-count":41,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T00:00:00Z","timestamp":1601596800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018648","name":"Phileo by Lesaffre","doi-asserted-by":"publisher","award":["401"],"award-info":[{"award-number":["401"]}],"id":[{"id":"10.13039\/501100018648","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The present study with 1-day-old male broilers (Ross 308) was conducted to evaluate the effects of deoxynivalenol (DON) at different levels (5 and 15 mg\/kg feed) on growth performance, relative weight of organs, morphology of the small intestine, serum biochemistry, and welfare parameters of broiler chickens. Forty-five broiler chicks were randomly divided into three different experimental groups with five replicates each: (1) control group received a non-contaminated diet, (2) contaminated diet with 5 mg DON\/kg of feed, and (3) contaminated diet with 15 mg DON\/kg of feed for 42 days. Results showed that feed artificially contaminated with DON at guidance level (5 mg\/kg diet) did not affect growth performance parameters. However, 15 mg\/kg reduced body weight gain and altered feed efficiency. DON at two assayed levels significantly increased the absolute and relative weight of thymus and the relative weight of gizzard and decreased the absolute and the relative weight of the colon. Compared to controls, both doses affected small intestine morphometry parameters. In terms of biochemical indicators, DON at 5 mg\/kg reduced the creatine kinase level and at 15 mg\/kg DON reduced the cholesterol level. Furthermore, DON at 15 mg\/kg induced more fear in broilers compared to broilers fed the guidance level. It was concluded that even the guidance level of DON did not affect the chickens\u2019 performance. However, its toxic effect occurred in some organs and biochemical parameters.<\/jats:p>","DOI":"10.3390\/ani10101795","type":"journal-article","created":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T07:22:16Z","timestamp":1601709736000},"page":"1795","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Effects of Deoxynivalenol-Contaminated Diets on Productive, Morphological, and Physiological Indicators in Broiler Chickens"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5958-2311","authenticated-orcid":false,"given":"Insaf","family":"Riahi","sequence":"first","affiliation":[{"name":"Institute of Agrifood Research and Technology (IRTA Mas Bov\u00e9), Animal Nutrition Department, 43120 Constanti, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3609-3731","authenticated-orcid":false,"given":"Virginie","family":"Marquis","sequence":"additional","affiliation":[{"name":"Phileo by Lesaffre, 137 rue Gabriel P\u00e9ri, 59700 Marcq en Baroeul, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2830-8299","authenticated-orcid":false,"given":"Antonio J.","family":"Ramos","sequence":"additional","affiliation":[{"name":"Applied Mycology Unit, Food Technology Department, University of Lleida, UTPV-XaRTA, Agrotecnio, Av.Rovira Roure 191, 25198 Lleida, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaquim","family":"Brufau","sequence":"additional","affiliation":[{"name":"Institute of Agrifood Research and Technology (IRTA Mas Bov\u00e9), Animal Nutrition Department, 43120 Constanti, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5942-724X","authenticated-orcid":false,"given":"Enric","family":"Esteve-Garcia","sequence":"additional","affiliation":[{"name":"Institute of Agrifood Research and Technology (IRTA Mas Bov\u00e9), Animal Nutrition Department, 43120 Constanti, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9332-2425","authenticated-orcid":false,"given":"Anna Maria","family":"P\u00e9rez-Vendrell","sequence":"additional","affiliation":[{"name":"Institute of Agrifood Research and Technology (IRTA Mas Bov\u00e9), Animal Nutrition Department, 43120 Constanti, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0378-4274(01)00479-9","article-title":"Update of survey, regulation and toxic effects of mycotoxins in Europe","volume":"127","author":"Creppy","year":"2002","journal-title":"Toxicol. Lett."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.fct.2015.02.005","article-title":"In vivo toxicity studies of fusarium mycotoxins in the last decade: A review","volume":"78","author":"Font","year":"2015","journal-title":"Food Chem. Toxicol."},{"key":"ref_3","unstructured":"Biomin (2020, June 12). BIOMIN World Mycotoxin Survey 2018. Available online: https:\/\/www.romerlabs.com\/en\/knowledge-center\/knowledge-library\/articles\/news\/biomin-world-mycotoxin-survey-2018\/."},{"key":"ref_4","unstructured":"(2006). European Commission Commission Recommendation of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins inproducts intended for animal feeding. Off. J. Eur. Union, L299, 7\u20139."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Chen, S.S., Li, Y.H., and Lin, M.F. (2017). Chronic exposure to the fusarium mycotoxin deoxynivalenol: Impact on performance, immune organ, and intestinal integrity of slow-growing chickens. Toxins, 9.","DOI":"10.3390\/toxins9100334"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s12550-017-0284-z","article-title":"Effects of low to moderate levels of deoxynivalenol on feed and water intake, weight gain, and slaughtering traits of broiler chickens","volume":"33","author":"Lucke","year":"2017","journal-title":"Mycotoxin Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ghareeb, K., Awad, W.A., Sid-Ahmed, O.E., and B\u00f6hm, J. (2014). Insights on the host stress, fear and growth responses to the deoxynivalenol feed contaminant in broiler chickens. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0087727"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/jpn.12328","article-title":"Deoxynivalenol in chicken feed alters the vaccinal immune response and clinical biochemical serum parameters but not the intestinal and carcass characteristics","volume":"100","author":"Ghareeb","year":"2016","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1964","DOI":"10.1093\/ps\/83.12.1964","article-title":"Effects of deoxynivalenol on general performance and electrophysiological properties of intestinal mucosa of broiler chickens","volume":"83","author":"Awad","year":"2004","journal-title":"Poult. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1093\/ps\/85.6.974","article-title":"Effect of addition of a probiotic microorganism to broiler diets contaminated with deoxynivalenol on performance and histological alterations of intestinal villi of broiler chickens","volume":"85","author":"Awad","year":"2006","journal-title":"Poult. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.3382\/ps.0651291","article-title":"Individual and combined effects of aflatoxin and deoxynivalenol (DON), vomitoxin in broiler chickens","volume":"65","author":"Huff","year":"1986","journal-title":"Poult. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"622","DOI":"10.3382\/ps.0680622","article-title":"Individual and combined toxicity of deoxynivalenol and T-2 toxin in broiler chicks","volume":"68","author":"Kubena","year":"1989","journal-title":"Poult. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1093\/ps\/76.9.1239","article-title":"Individual and Combined Effects of Fumonisin B1 Present in Fusarium moniliforme Culture Material and T-2 Toxin or Deoxynivalenol in Broiler Chicks","volume":"76","author":"Kubena","year":"1997","journal-title":"Poult. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0071492","article-title":"Effects of Feed Contaminant Deoxynivalenol on Plasma Cytokines and mRNA Expression of Immune Genes in the Intestine of Broiler Chickens","volume":"8","author":"Ghareeb","year":"2013","journal-title":"PLoS ONE"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"800","DOI":"10.3382\/ps.2011-01741","article-title":"Ameliorative effect of a microbial feed additive on infectious bronchitis virus antibody titer and stress index in broiler chicks fed deoxynivalenol","volume":"91","author":"Ghareeb","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2766","DOI":"10.3382\/ps.2011-01654","article-title":"Effects of increasing dietary concentrations of corn naturally contaminated with deoxynivalenol on broiler and Turkey poult performance and response to lipopolysaccharide","volume":"90","author":"Xu","year":"2011","journal-title":"Poult. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1093\/ps\/84.7.1004","article-title":"Influence of perches and footpad dermatitis on tonic immobility and heterophil to lymphocyte ratio of chickens","volume":"84","author":"Campo","year":"2005","journal-title":"Poult. Sci."},{"key":"ref_18","unstructured":"NRC (1994). Nutrient Requirements of Poultry, National Academies Press. [9th ed.]."},{"key":"ref_19","unstructured":"Association of Official Analytical Chemists (AOAC) (2004). Official Methods of Analysis, AOAC. [17th ed.]."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Metayer, J.P., Travel, A., Mika, A., Bailly, J.D., Cleva, D., Boissieu, C., Guennec, J.L., Froment, P., Albaric, O., and Labrut, S. (2019). Lack of toxic interaction between fusariotoxins in broiler chickens fed throughout their life at the highest level tolerated in the european union. Toxins, 11.","DOI":"10.3390\/toxins11080455"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.2527\/jas.2005-414","article-title":"Effects of spray-dried porcine plasma and plant extracts on intestinal morphology and on leukocyte cell subsets of weaned pigs","volume":"84","author":"Manzanilla","year":"2006","journal-title":"J. Anim. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1111\/j.1439-0396.2005.00616.x","article-title":"Effects of feeding deoxynivalenol contaminated wheat on growth performance, organ weights and histological parameters of the intestine of broiler chickens","volume":"90","author":"Awad","year":"2006","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1111\/jpn.12883","article-title":"An impact of Deoxynivalenol produced by Fusarium graminearum on broiler chickens","volume":"102","author":"Yu","year":"2018","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1007\/s00204-016-1710-2","article-title":"In vivo contribution of deoxynivalenol-3-\u03b2-d-glucoside to deoxynivalenol exposure in broiler chickens and pigs: Oral bioavailability, hydrolysis and toxicokinetics","volume":"91","author":"Broekaert","year":"2017","journal-title":"Arch. Toxicol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.fct.2012.10.006","article-title":"Toxicokinetic study and absolute oral bioavailability of deoxynivalenol, T-2 toxin and zearalenone in broiler chickens","volume":"51","author":"Osselaere","year":"2013","journal-title":"Food Chem. Toxicol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4706","DOI":"10.3390\/toxins7114706","article-title":"Metabolism of deoxynivalenol and deepoxy-deoxynivalenol in broiler chickens, pullets, roosters and turkeys","volume":"7","author":"Fruhmann","year":"2015","journal-title":"Toxins"},{"key":"ref_27","first-page":"27","article-title":"Biochemical Mechanism of Action of Trichothecene Mycotoxins","volume":"Volume I","author":"Beasley","year":"1989","journal-title":"Trichothecene Mycotoxicosis: Pathophysiologic Effects"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0088028","article-title":"Single and combined effects of deoxynivalenol mycotoxin and a microbial feed additive on lymphocyte DNA damage and oxidative stress in broiler chickens","volume":"9","author":"Awad","year":"2014","journal-title":"PLoS ONE"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.3382\/ps.0641649","article-title":"Effects of Feeding Deoxynivalenol (Vomitoxin)-Contaminated Wheat to growing chicks","volume":"64","author":"Kubena","year":"1985","journal-title":"Poult.Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.3382\/ps.0651095","article-title":"Effects of feeding hens a high level of vomitoxin-contaminated corn on performance and tissue residues","volume":"65","author":"Lun","year":"1986","journal-title":"Poult. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7996","DOI":"10.3390\/ijms12117996","article-title":"The impact of the Fusarium mycotoxin deoxynivalenol on the health and performance of broiler chickens","volume":"12","author":"Awad","year":"2011","journal-title":"Int. J. Mol. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"844","DOI":"10.3382\/ps.2011-01873","article-title":"Deoxynivalenol as a contaminant of broiler feed: Effects on bird performance and response to common vaccines","volume":"91","author":"Yunus","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"852","DOI":"10.3382\/ps.2011-01903","article-title":"Deoxynivalenol as a contaminant of broiler feed: Intestinal development, absorptive functionality, and metabolism of the mycotoxin","volume":"91","author":"Yunus","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"827","DOI":"10.3923\/ijps.2008.827.842","article-title":"The impact of the Fusarium toxin deoxynivalenol (DON) on poultry","volume":"7","author":"Awad","year":"2008","journal-title":"Int. J. Poult. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/009841096161447","article-title":"Toxicology of deoxynivalenol (vomitoxin)","volume":"48","author":"Rotter","year":"1996","journal-title":"J. Toxicol. Environ. Health"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"7509","DOI":"10.1039\/C9FO01863H","article-title":"GABA attenuates ETEC-induced intestinal epithelial cell apoptosis involving GABA AR signaling and the AMPK autophagy pathway","volume":"10","author":"Xia","year":"2019","journal-title":"Food Funct."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.3390\/toxins7062071","article-title":"Deoxynivalenol impairs weight gain and affects markers of gut health after low-dose, short-term exposure of growing pigs","volume":"7","author":"Alizadeh","year":"2015","journal-title":"Toxins"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.3382\/ps.0661612","article-title":"Effects of feeding deoxynivalenol (DON, vomitoxin)-contaminated wheat to female White Leghorn chickens from day old through egg production","volume":"66","author":"Kubena","year":"1987","journal-title":"Poult. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"376","DOI":"10.3382\/ps.2011-01813","article-title":"Meta-analysis of the relationship of mycotoxins with biochemical and hematological parameters in broilers","volume":"91","author":"Andretta","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/0006-2944(83)90068-6","article-title":"The levels of creatine kinase and adenylate kinase in the plasma of dystrophic chickens reflect the rates of loss of these enzymes from the circulation","volume":"29","author":"Husic","year":"1983","journal-title":"Biochem. Med."},{"key":"ref_41","first-page":"2131","article-title":"Effects of feeding blends of grains naturally contaminated with Fusarium mycotoxins on barin regional neurochemistry of starter pigs and broiler chickens","volume":"82","author":"Swamy","year":"2004","journal-title":"Poult. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/10\/1795\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:16:05Z","timestamp":1760177765000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/10\/1795"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,2]]},"references-count":41,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2020,10]]}},"alternative-id":["ani10101795"],"URL":"https:\/\/doi.org\/10.3390\/ani10101795","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,2]]}}}