{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T07:19:28Z","timestamp":1768893568538,"version":"3.49.0"},"reference-count":39,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,4]],"date-time":"2020-10-04T00:00:00Z","timestamp":1601769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["\u0410\u0410\u0410\u0410-\u041018-118021990006-9"],"award-info":[{"award-number":["\u0410\u0410\u0410\u0410-\u041018-118021990006-9"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The fertilizing ability of stallion sperm after freezing is lower than in other species. The search for the optimal extender, combination of extenders, and the freezing protocol is relevant. The aim of this study was to compare lactose-chelate-citrate-yolk (LCCY) extender, usually used in Russia, and Steridyl\u00ae (Minitube) for freezing sperm of stallions. Steridyl is a concentrated extender medium for freezing ruminant semen. It already contains sterilized egg yolk. Semen was collected from nine stallions, aged from 7 to 12 years old. The total and progressive motility of sperm frozen in Steridyl was significantly higher than in semen frozen in LCCY. The number of spermatozoa with normal morphology in samples frozen in LCCY was 60.4 \u00b1 1.72%, and with Steridyl, 72.4 \u00b1 2.10% (p &lt; 0.01). Semen frozen in Steridyl showed good stimulation of respiration by 2.4-DNP, which indicates that oxidative phosphorylation was retained after freezing\u2013thawing. No differences among the extenders were seen with the DNA integrity of spermatozoa. Six out of ten (60%) mares were pregnant after artificial insemination (AI) by LCCY frozen semen, and 9\/12 (75%) by Steridyl frozen semen. No differences among extenders were seen in pregnancy rate. In conclusion, Steridyl was proven to be a good diluent for freezing stallion semen, even though it was developed for ruminants.<\/jats:p>","DOI":"10.3390\/ani10101801","type":"journal-article","created":{"date-parts":[[2020,10,5]],"date-time":"2020-10-05T08:35:57Z","timestamp":1601886957000},"page":"1801","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions"],"prefix":"10.3390","volume":"10","author":[{"given":"Elena","family":"Nikitkina","sequence":"first","affiliation":[{"name":"Russian Research Institute for Farm Animal Genetics and Breeding\u2014Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Moskovskoye sh. 55A, St. Petersburg, Pushkin 196625, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Artem","family":"Musidray","sequence":"additional","affiliation":[{"name":"Russian Research Institute for Farm Animal Genetics and Breeding\u2014Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Moskovskoye sh. 55A, St. Petersburg, Pushkin 196625, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2561-145X","authenticated-orcid":false,"given":"Anna","family":"Krutikova","sequence":"additional","affiliation":[{"name":"Russian Research Institute for Farm Animal Genetics and Breeding\u2014Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Moskovskoye sh. 55A, St. Petersburg, Pushkin 196625, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Polina","family":"Anipchenko","sequence":"additional","affiliation":[{"name":"Russian Research Institute for Farm Animal Genetics and Breeding\u2014Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Moskovskoye sh. 55A, St. Petersburg, Pushkin 196625, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirill","family":"Plemyashov","sequence":"additional","affiliation":[{"name":"Russian Research Institute for Farm Animal Genetics and Breeding\u2014Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Moskovskoye sh. 55A, St. Petersburg, Pushkin 196625, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gennadiy","family":"Shiryaev","sequence":"additional","affiliation":[{"name":"Russian Research Institute for Farm Animal Genetics and Breeding\u2014Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Moskovskoye sh. 55A, St. Petersburg, Pushkin 196625, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.jevs.2012.06.011","article-title":"Artificial Insemination in Horses-More than a Century of Practice and Research","volume":"32","author":"Aurich","year":"2012","journal-title":"J. Equine Vet. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.anireprosci.2007.11.010","article-title":"Commercial semen freezing: Individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols","volume":"105","author":"Loomis","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0378-4320(00)00214-1","article-title":"Morphological and functional changes of stallion spermatozoa after cryopreservation during breeding and non-breeding season","volume":"65","author":"Blottner","year":"2001","journal-title":"Anim. Reprod. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1111\/rda.13611","article-title":"Cryopreservation of stallion semen: Effect of adding antioxidants to the freezing medium on sperm physiology","volume":"55","author":"Contreras","year":"2020","journal-title":"Reprod. Domest. Anim."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1111\/j.1751-0813.2007.00151.x","article-title":"A comparison of duck and chicken egg yolk for cryopreservation of stallion sperm","volume":"85","author":"Clulow","year":"2007","journal-title":"Aust. Vet. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1111\/j.1751-0813.2009.00452.x","article-title":"Freezing equine semen: The effect of combinations of semen extenders and glycerol on post-thaw motility","volume":"87","author":"Scherzer","year":"2009","journal-title":"Aust. Vet. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"78","DOI":"10.24189\/ncr.2019.024","article-title":"Conservation of genetic resources in horse breeding and major structural damages of sperm during semen cryopreservation in stallions","volume":"4","author":"Atroshchenko","year":"2019","journal-title":"Nat. Conserv. Res."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Atroshchenko, M.M., Arkhangelskaya, E., Isaev, D.A., Stavitsky, S.B., Zaitsev, A.M., Kalaschnikov, V.V., Leonov, S., and Osipov, A.N. (2019). Reproductive characteristics of thawed stallion sperm. Animals, 9.","DOI":"10.3390\/ani9121099"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.anireprosci.2005.07.001","article-title":"Amides as cryoprotectants for freezing stallion semen: A review","volume":"89","author":"Alvarenga","year":"2005","journal-title":"Anim. Reprod. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"880","DOI":"10.2460\/ajvr.2002.63.880","article-title":"Effect of seminal plasma concentration and various extenders on postthaw motility and glass wool-Sephadex filtration of cryopreserved stallion semen","volume":"63","author":"Alghamdi","year":"2002","journal-title":"Am. J. Vet. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.2527\/jas1974.3961144x","article-title":"Components in egg yolk which protect bovine spermatozoa during freezing","volume":"39","author":"Pace","year":"1974","journal-title":"J. Anim. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0378-4320(01)00155-5","article-title":"Advances in cooled semen technology","volume":"68","author":"Batellier","year":"2001","journal-title":"Anim. Reprod. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.anireprosci.2017.06.008","article-title":"Supplementation of tris-based extender with plasma egg yolk of six avian species and camel skim milk for chilled preservation of dromedary camel semen","volume":"184","author":"Panahi","year":"2017","journal-title":"Anim. Reprod. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.theriogenology.2018.12.018","article-title":"Ostrich egg yolk improves post thaw quality and in vivo fertility of Nili Ravi buffalo (Bubalus bubalis) bull spermatozoa","volume":"126","author":"Naz","year":"2019","journal-title":"Theriogenology"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Swelum, A.A.A., Saadeldin, I.M., Ba-Awadh, H., Al-Mutary, M.G., Moumen, A.F., Alowaimer, A.N., and Abdalla, H. (2019). Efficiency of commercial egg yolk-free and egg yolk-supplemented tris-based extenders for dromedary camel semen cryopreservation. Animals, 9.","DOI":"10.3390\/ani9110999"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1002\/j.1939-4640.2003.tb02641.x","article-title":"The sperm chromatin dispersion test: A simple method for the determination of sperm DNA fragmentation","volume":"24","author":"Muriel","year":"2003","journal-title":"J. Androl."},{"key":"ref_17","first-page":"28","article-title":"Polarographic method for assessing energy metabolism in sperm by stimulating respiration with 2.4-dinitrophenol","volume":"33","author":"Moroz","year":"1978","journal-title":"Bull. State Sci. Inst. All-Russian Res. Inst. Farm Anim. Genet. Breed."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1016\/j.theriogenology.2011.01.014","article-title":"Improved cryopreservability of stallion sperm using a sorbitol-based freezing extender","volume":"75","author":"Pojprasath","year":"2011","journal-title":"Theriogenology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/S0093-691X(97)00319-1","article-title":"Equine frozen semen: Freezability and fertility field results","volume":"48","author":"Vidament","year":"1997","journal-title":"Theriogenology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.anireprosci.2005.07.003","article-title":"French field results (1985\u20132005) on factors affecting fertility of frozen stallion semen","volume":"89","author":"Vidament","year":"2005","journal-title":"Anim. Reprod. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"\u0160ichta\u0159, J., Buben\u00ed\u010dkov\u00e1, F., Sirohi, J., and \u0160imon\u00edk, O. (2019). How to increase post-thaw semen quality in poor freezing stallions: Preliminary results of the promising role of seminal plasma added after thawing. Animals, 9.","DOI":"10.3390\/ani9070414"},{"key":"ref_22","first-page":"109","article-title":"A comparison of duck and chicken egg yolk for cryopreservation of Egyptian buffalo bull spermatozoa Scientific Papers","volume":"55","author":"Hussein","year":"2012","journal-title":"Sci. Pap. Ser. D Anim. Sci."},{"key":"ref_23","unstructured":"Minitube Insemination Results When Applying Triladyl\u00ae or Steridyl as Preservation Media for Bull Semen (2012). Technical Report, Minitube."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1016\/j.theriogenology.2016.04.001","article-title":"Development of a new fertility prediction model for stallion semen, including flow cytometry","volume":"86","author":"Kempfer","year":"2016","journal-title":"Theriogenology"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Prien, S. (2016). Cryoprotectants & Cryopreservation of Equine Semen: A Review of Industry Cryoprotectants and the Effects of Cryopreservation on Equine Semen Membranes. J. Dairy Vet. Anim. Res., 3.","DOI":"10.15406\/jdvar.2016.03.00063"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"34","DOI":"10.14202\/vetworld.2019.34-40","article-title":"Effects of four extenders on the quality of frozen semen in Arabian stallions","volume":"12","author":"Alamaary","year":"2019","journal-title":"Vet. World"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"S37","DOI":"10.1016\/j.jevs.2016.06.075","article-title":"Approaches to Breeding Soundness Examination and Interpretation of Results","volume":"43","author":"Varner","year":"2016","journal-title":"J. Equine Vet. Sci."},{"key":"ref_28","first-page":"146","article-title":"the Effect of Different Freezing Procedures on Sperm Head Morphometry in Stallions","volume":"65","author":"Teodora","year":"2008","journal-title":"Bull. Univ. Agric. Sci. Vet. Med. Cluj-Napoca Vet. Med."},{"key":"ref_29","first-page":"49","article-title":"Assessment of the respiratory activity in equine sperm. Reproduction in Domestic Animals","volume":"49","author":"Nikitkina","year":"2014","journal-title":"Reprod. Domest. Anim."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.anireprosci.2018.03.024","article-title":"The sperm mitochondrion: Organelle of many functions","volume":"194","author":"Moraes","year":"2018","journal-title":"Anim. Reprod. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1530\/REP-16-0409","article-title":"Mitochondrial ATP is required for the maintenance of membrane integrity in stallion spermatozoa, whereas motility requires both glycolysis and oxidative phosphorylation","volume":"152","author":"Davila","year":"2016","journal-title":"Reproduction"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Gibb, Z., Lambourne, S.R., and Aitken, R.J. (2014). The paradoxical relationship between stallion fertility and oxidative stress. Biol. Reprod., 91.","DOI":"10.1095\/biolreprod.114.118539"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"105","DOI":"10.2164\/jandrol.110.011957","article-title":"The mitochondria of stallion spermatozoa are more sensitive than the plasmalemma to osmotic-induced stress: Role of c-Jun N-terminal kinase (JNK) pathway","volume":"33","author":"Moran","year":"2012","journal-title":"J. Androl."},{"key":"ref_34","first-page":"121","article-title":"Correlation between the level of respiratory stimulation by 2,4-dnp and fertilizing ability of frozen sperm","volume":"51","author":"Nikitkina","year":"2016","journal-title":"Reprod. Domest. Anim."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1023\/A:1009844109023","article-title":"Sperm chromatin structure assay is useful for fertility assessment","volume":"22","author":"Evenson","year":"2000","journal-title":"Methods Cell Sci."},{"key":"ref_36","first-page":"1","article-title":"Current trends on stallion semen evaluation: What other methods can be used to improve our capacity for semen assessement?","volume":"4","year":"2019","journal-title":"J. Vet. Androl."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1093\/humrep\/deg228","article-title":"Sperm DNA fragentation decreases the pregnancy rate in an assisted reproductive technique","volume":"18","author":"Benchaib","year":"2003","journal-title":"Hum. Reprod."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Morrell, J.M., Johannisson, A., Dalin, A.M., Hammar, L., Sandebert, T., and Rodriguez-Martinez, H. (2008). Sperm morphology and chromatin integrity in Swedish warmblood stallions and their relationship to pregnancy rates. Acta Vet. Scand., 50.","DOI":"10.1186\/1751-0147-50-2"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Burruel, V., Klooster, K.L., Chitwood, J., Ross, P.J., and Meyers, S.A. (2013). Oxidative damage to rhesus macaque spermatozoa results in mitotic arrest and transcript abundance changes in early embryos. Biol. Reprod., 89.","DOI":"10.1095\/biolreprod.113.110981"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/10\/1801\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:16:25Z","timestamp":1760177785000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/10\/1801"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,4]]},"references-count":39,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2020,10]]}},"alternative-id":["ani10101801"],"URL":"https:\/\/doi.org\/10.3390\/ani10101801","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,4]]}}}