{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:13:59Z","timestamp":1791238439995,"version":"4.1.0"},"reference-count":22,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,12]],"date-time":"2020-10-12T00:00:00Z","timestamp":1602460800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>In spring and summer 2020, six outbreaks of condemnation of jaundiced lamb carcasses were diagnosed in different farms in Arag\u00f3n region, Spain. Anaplasma ovis was identified in all affected farms. Four hundred and ninety-two lambs from two affected farms were more closely examined. Clinical examination, haematologies, biochemistries, histopathology and microbiological and molecular analyses were performed. After slaughter, 34.84% of the lambs showed jaundiced carcasses and 79.64% presented splenomegaly at the abattoir. All tested lambs with icteric carcasses showed positive A. ovis PCR, although 72.72% of the unaffected lambs also tested positive. However, the bacterial load was significantly higher in the animals that showed jaundiced carcasses (Cq: 25.00 vs 26.16; p = 0.004). Moreover, all the tested lambs that showed severe anaemia were PCR positive. On the contrary, the PCR negative lambs did not show anaemia. Lambs that presented icteric carcasses displayed severe regenerative anaemia with significantly lower erythrocyte count (7.18 vs. 11.97), haematocrit (26.89 vs. 34.82) and haemoglobin (8.50 vs. 11.10) than unaffected lambs. Reticulocyte count (18.80 vs. 5.65) was also significantly increased in affected animals. This article describes a new disorder caused by Anaplasma ovis that is producing significant economic losses associated with the carcass condemnation of apparently healthy lamb.<\/jats:p>","DOI":"10.3390\/ani10101851","type":"journal-article","created":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T21:42:21Z","timestamp":1602798141000},"page":"1851","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Anaplasmosis Outbreak in Lambs: First Report Causing Carcass Condemnation"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7822-6646","authenticated-orcid":false,"given":"Delia","family":"Lacasta","sequence":"first","affiliation":[{"name":"Animal Pathology Department, Instituto Agroalimentario de Arag\u00f3n-IA2 (Universidad de Zaragoza-CITA), Veterinary Faculty of Zaragoza, C\/Miguel Servet 177, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Miguel","family":"Ferrer","sequence":"additional","affiliation":[{"name":"Animal Pathology Department, Instituto Agroalimentario de Arag\u00f3n-IA2 (Universidad de Zaragoza-CITA), Veterinary Faculty of Zaragoza, C\/Miguel Servet 177, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"Sanz","sequence":"additional","affiliation":[{"name":"ADS Nuestra Sra, del Pueyo, C\/Pilar 21, 50130 Belchite, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Labanda","sequence":"additional","affiliation":[{"name":"Casa de Ganaderos de Zaragoza, C\/San Andr\u00e9s, 8, 50001 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4655-9171","authenticated-orcid":false,"given":"Jos\u00e9 Mar\u00eda","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Animal Pathology Department, Instituto Agroalimentario de Arag\u00f3n-IA2 (Universidad de Zaragoza-CITA), Veterinary Faculty of Zaragoza, C\/Miguel Servet 177, 50013 Zaragoza, Spain"},{"name":"Gabinete T\u00e9cnico Veterinario S.L, C\/Isla conejera sn, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2497-9028","authenticated-orcid":false,"given":"Alfredo \u00c1ngel","family":"Benito","sequence":"additional","affiliation":[{"name":"Exopol S.L. Pol. R\u00edo G\u00e1llego D-8, San Mateo de G\u00e1llego, 50840 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8474-2831","authenticated-orcid":false,"given":"H\u00e9ctor","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Animal Pathology Department, Instituto Agroalimentario de Arag\u00f3n-IA2 (Universidad de Zaragoza-CITA), Veterinary Faculty of Zaragoza, C\/Miguel Servet 177, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Rodr\u00edguez-Largo","sequence":"additional","affiliation":[{"name":"Animal Pathology Department, Instituto Agroalimentario de Arag\u00f3n-IA2 (Universidad de Zaragoza-CITA), Veterinary Faculty of Zaragoza, C\/Miguel Servet 177, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Jos\u00e9","family":"Ramos","sequence":"additional","affiliation":[{"name":"Animal Pathology Department, Instituto Agroalimentario de Arag\u00f3n-IA2 (Universidad de Zaragoza-CITA), Veterinary Faculty of Zaragoza, C\/Miguel Servet 177, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"573","DOI":"10.17221\/1861-VETMED","article-title":"Bacteria of the genus Anaplasma\u2013characteristics of Anaplasma and their vectors: A review","volume":"53","author":"Rymaszewska","year":"2008","journal-title":"Vet. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"30","DOI":"10.14737\/journal.jap\/2015\/2.2.30.34","article-title":"Epidemiological study of sheep anaplasmosis (Anaplasma ovis infection) in Kairouan, Central Tunisia","volume":"2","author":"Gharbi","year":"2015","journal-title":"J. Adv. Parasitol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.vetmic.2007.01.024","article-title":"First serological and molecular evidence on the endemicity of Anaplasma ovis and A. marginale in Hungary","volume":"122","author":"Hornok","year":"2007","journal-title":"Vet. Microbial."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.smallrumres.2016.03.005","article-title":"Haemoparasites in small ruminants in European countries: Challenges and clinical relevance","volume":"142","author":"Stuen","year":"2016","journal-title":"Small Rumin. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/tbed.12149","article-title":"Can Anaplasma ovis in small ruminants be neglected any longer?","volume":"60","author":"Renneker","year":"2013","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.smallrumres.2019.07.003","article-title":"Anaplasma ovis in sheep: Experimental infection, vertical transmission and colostral immunity","volume":"178","author":"Benito","year":"2019","journal-title":"Small Rumin. Res."},{"key":"ref_7","first-page":"115","article-title":"Tick-borne diseases of sheep and goats and their related vectors in Iran","volume":"39","year":"1997","journal-title":"Parassitologia"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Cabezas-Cruz, A., Gallois, M., Fontugne, M., Allain, E., Denoual, M., Moutailler, S., Devillers, E., Zientara, S., Memmi, M., and Chauvin, A. (2019). Epidemiology and genetic diversity of Anaplasma ovis in goats in Corsica, France. Parasites Vectors, 12.","DOI":"10.1186\/s13071-018-3269-7"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.rvsc.2007.05.018","article-title":"Evidence of Anaplasma infections in European roe deer (Capreolus capreolus) from Southern Spain","volume":"84","author":"Naranjo","year":"2008","journal-title":"Res. Vet. Sci."},{"key":"ref_10","first-page":"43","article-title":"Blood Parasitic Diseases and Specific Immune Responses","volume":"OIE","author":"Masake","year":"2010","journal-title":"Int. Livest. Res. Inst."},{"key":"ref_11","first-page":"315","article-title":"La respuesta inmune celular en anaplasmosis bovina","volume":"7","author":"Bautista","year":"1996","journal-title":"Cienc. Vet."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.cimid.2011.12.002","article-title":"Adaptive immunity to Anaplasma pathogens and immune dysregulation: Implications for bacterial persistence","volume":"35","author":"Brown","year":"2012","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.ttbdis.2012.10.033","article-title":"Development and validation of two PCR tests for the detection of and differentiation between Anaplasma ovis and Anaplasma marginale","volume":"3","author":"Torina","year":"2012","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1136\/inp.m1462","article-title":"Clostridial diseases in farm animals: 1. Enterotoxaemias and other alimentary tract infections","volume":"42","author":"Otter","year":"2020","journal-title":"Practice"},{"key":"ref_16","first-page":"99","article-title":"Tick-borne diseases of sheep and goats caused by Babesia, Theileria or Anaplasma spp.","volume":"39","author":"Friedhoff","year":"1997","journal-title":"Parassitologia"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"104750","DOI":"10.1016\/j.prevetmed.2019.104750","article-title":"Mycoplasma ovis infection in domestic sheep (Ovis aries) in the United States: Prevalence, distribution, associated risk factors, and associated outcomes","volume":"171","author":"Urie","year":"2019","journal-title":"Prev. Vet. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.12681\/jhvms.18866","article-title":"Diagnostic approach of anemia in ruminants","volume":"69","author":"Katsogiannou","year":"2018","journal-title":"J. Hell. Vet. Med. Soc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.vetpar.2011.05.032","article-title":"Emerging perspectives in the research of bovine babesiosis and anaplasmosis","volume":"180","author":"Suarez","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/j.1751-0813.2008.00382.x","article-title":"Haematological, biochemical and selected acute phase protein reference intervals for weaned female Merino lambs","volume":"87","author":"Lepherd","year":"2009","journal-title":"Aust. Vet. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"S6","DOI":"10.1016\/j.smallrumres.2012.04.026","article-title":"Tick-borne diseases in sheep and goats: Clinical and diagnostic aspects","volume":"106","author":"Alessandra","year":"2012","journal-title":"Small Rumin. Res."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Boes, K.M., and Durham, A.C. (2017). Bone Marrow, Blood Cells, and the Lymphoid\/Lymphatic System. Pathologic Basis of Veterinary Diseases, Elsevier. [6th ed.].","DOI":"10.1016\/B978-0-323-35775-3.00013-8"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/10\/1851\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:19:29Z","timestamp":1760177969000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/10\/1851"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,12]]},"references-count":22,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2020,10]]}},"alternative-id":["ani10101851"],"URL":"https:\/\/doi.org\/10.3390\/ani10101851","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,12]]}}}