{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T04:48:43Z","timestamp":1772945323131,"version":"3.50.1"},"reference-count":49,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T00:00:00Z","timestamp":1603238400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["773830"],"award-info":[{"award-number":["773830"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Salmonella spp. contaminates egg and poultry meat leading to foodborne infections in humans. The emergence of antimicrobial-resistant strains has limited the use of antimicrobials. We aimed to determine the effects of the food supplement, fermented defatted \u2018alperujo\u2019 (FDA), a modified olive oil by-product, on Salmonella Typhimurium colonisation in broilers. One hundred and twenty 1-day-old broilers were divided into four experimental groups\u2014two control groups and two treated groups, and challenged with S. Typhimurium at day 7 or 21. On days 7, 14, 21, 28, 35, and 42 of life, duodenum and cecum tissue samples were collected for histopathological and histomorphometric studies. Additionally, cecum content was collected for Salmonella spp. detection by culture and qPCR, and for metagenomic analysis. Our results showed a significant reduction of Salmonella spp. in the cecum of 42-day-old broilers, suggesting that fermented defatted \u2018alperujo\u2019 limits Salmonella Typhimurium colonization in that cecum and may contribute to diminishing the risk of carcass contamination at the time of slaughter. The improvement of the mucosal integrity, observed histologically and morphometrically, may contribute to enhancing intestinal health and to limiting Salmonella spp. colonisation in the host, mitigating production losses. These results could provide evidence that FDA would contribute to prophylactic and therapeutic measures to reduce salmonellosis prevalence in poultry farms.<\/jats:p>","DOI":"10.3390\/ani10101931","type":"journal-article","created":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T20:51:00Z","timestamp":1603399860000},"page":"1931","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Reduction of Salmonella Typhimurium Cecal Colonisation and Improvement of Intestinal Health in Broilers Supplemented with Fermented Defatted \u2018Alperujo\u2019, an Olive Oil By-Product"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4304-2713","authenticated-orcid":false,"given":"Agust\u00edn","family":"Rebollada-Merino","sequence":"first","affiliation":[{"name":"VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Department of Internal Medicine and Animal Surgery, Faculty of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4509-6021","authenticated-orcid":false,"given":"Mar\u00eda","family":"Ugarte-Ruiz","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular y Microbiolog\u00eda, Instituto Tecnol\u00f3gico Agrario de Castilla y Le\u00f3n, 47071 Valladolid, Spain"},{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Biotecnolog\u00eda y Ciencia de los Alimentos, Universidad de Burgos, 09001 Burgos, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Miguela-Villoldo","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Department of Animal Health, Faculty of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5423-3537","authenticated-orcid":false,"given":"David","family":"Abad","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular y Microbiolog\u00eda, Instituto Tecnol\u00f3gico Agrario de Castilla y Le\u00f3n, 47071 Valladolid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8795-854X","authenticated-orcid":false,"given":"David","family":"Rodr\u00edguez-L\u00e1zaro","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular y Microbiolog\u00eda, Instituto Tecnol\u00f3gico Agrario de Castilla y Le\u00f3n, 47071 Valladolid, Spain"},{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Biotecnolog\u00eda y Ciencia de los Alimentos, Universidad de Burgos, 09001 Burgos, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc\u00eda","family":"de Juan","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Department of Animal Health, Faculty of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas","family":"Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Department of Animal Health, Faculty of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Rodr\u00edguez-Bertos","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Department of Internal Medicine and Animal Surgery, Faculty of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"EFSA (2017). 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