{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T03:15:24Z","timestamp":1774494924987,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T00:00:00Z","timestamp":1604102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Multiple ovulations and so multiple pregnancies have increased recently in dairy cattle. The incidence of the double ovulation impact in high producers at insemination may be over 20%. Twin pregnancies are undesirable as they seriously compromise the welfare and productive lifespan of the cow and herd economy. Clinical problems extend from the time of pregnancy diagnosis to pregnancy loss, abortion or parturition. Early pregnancy loss or abortion of multiple pregnancies lead in most cases to culling. In cows reaching their term, mean productive lifespan is up to about 300 days shorter for cows delivering twins than for cows delivering singletons. While there is an urgent need to address multiple pregnancy prevention procedures in the foreseeable future, the incidence of twin pregnancies continues to rise in parallel with increased milk production. Herein, we review two contrasting measures proposed for the time of twin pregnancy diagnosis: (1) gonadotropin-releasing hormone treatment for pregnancy maintenance, or (2) embryo reduction. These options are discussed in terms of their implications for individual animal health and herd economy. Our main conclusions find that manual twin reduction has proven to be the best management option, whereas the use of prostaglandin F2\u03b1 for inducing abortion may be a better option than doing nothing.<\/jats:p>","DOI":"10.3390\/ani10112006","type":"journal-article","created":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T21:39:56Z","timestamp":1604180396000},"page":"2006","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Twins in Dairy Herds. Is It Better to Maintain or Reduce a Pregnancy?"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1413-3862","authenticated-orcid":false,"given":"Fernando","family":"L\u00f3pez-Gatius","sequence":"first","affiliation":[{"name":"Bovine Reproduction SLu, 22300 Barbastro, Spain"},{"name":"Agrotecnio Centre, 25198 Lleida, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/j.jogoh.2018.09.007","article-title":"Fertility, fecundity and the creative instinct","volume":"47","author":"Hunter","year":"2018","journal-title":"J. Gynecol. Obstet. Hum. Reprod."},{"key":"ref_2","first-page":"221","article-title":"Cultic powers of Yoruba twins: Manifestation of traditional and religious beliefs of the Yoruba","volume":"32","author":"Oruene","year":"1983","journal-title":"Acta Genet. Med. Gemellol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1007\/s10900-005-9011-3","article-title":"Beliefs and practices concerning twins, hermaphrodites, and albinos among the Bamana and Maninka of Mali","volume":"31","author":"Imperato","year":"2006","journal-title":"J. Community Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"459","DOI":"10.2527\/2004.822459x","article-title":"Ovarian follicular development in cattle selected for twin ovulations and births","volume":"82","author":"Echternkamp","year":"2004","journal-title":"J. Anim. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1262\/jrd.2017-096","article-title":"The contribution of efficient production of monozygotic twins to beef cattle breeding","volume":"63","author":"Hashiyada","year":"2017","journal-title":"J. Reprod. Dev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.theriogenology.2004.03.006","article-title":"Spontaneous reduction of advanced twin embryos: Its occurrence and clinical relevance in dairy cattle","volume":"63","author":"Hunter","year":"2005","journal-title":"Theriogenology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1016\/j.theriogenology.2009.01.013","article-title":"Factors affecting pregnancy loss for single and twin pregnancies in a high-producing dairy herd","volume":"71","author":"Colloton","year":"2009","journal-title":"Theriogenology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.theriogenology.2009.09.012","article-title":"Factors affecting spontaneous reduction of corpora lutea and twin embryos during the late embryonic\/early fetal period in multiple-ovulating dairy cows","volume":"73","author":"Hunter","year":"2010","journal-title":"Theriogenology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1111\/j.1439-0531.2004.00533.x","article-title":"Timing of early foetal loss for single and twin pregnancies in dairy cattle","volume":"39","author":"Santolaria","year":"2004","journal-title":"Reprod. Domest. Anim."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.theriogenology.2011.03.003","article-title":"Clinical implications of induced twin reduction in dairy cattle","volume":"76","author":"Beckers","year":"2011","journal-title":"Theriogenology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8335","DOI":"10.3168\/jds.2018-14400","article-title":"An economic evaluation of management strategies to mitigate the negative effect of twinning in dairy herds","volume":"101","author":"Fricke","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_12","first-page":"93","article-title":"Twin pregnancy diagnosis in Holstein cows: Discriminatory powers and accuracy of diagnosis by transrectal palpation and outcome of twin pregnancies","volume":"36","author":"Day","year":"1995","journal-title":"Can. Vet. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.anireprosci.2005.03.003","article-title":"Genetic and environmental factors influencing first service conception rate and late embryonic\/foetal mortality in low fertility dairy herds","volume":"91","author":"Grimard","year":"2006","journal-title":"Anim. Reprod. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1111\/j.1439-0531.2010.01620.x","article-title":"Ultrasound and endocrine findings that help to assess the risk of late embryo\/early foetal loss by non-infectious cause in dairy cattle","volume":"45","year":"2010","journal-title":"Reprod. Domest. Anim."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"11798","DOI":"10.3168\/jds.2019-17176","article-title":"Predicting pregnancy loss in dairy cattle","volume":"102","author":"Ealy","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_16","first-page":"1295","article-title":"Ultrasonographic appearance of the bovine conceptus from days 20 through 60","volume":"189","author":"Curran","year":"1986","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.tvjl.2007.01.022","article-title":"Conceptus-related measurements during the first trimester of bovine pregnancy","volume":"175","author":"Riding","year":"2008","journal-title":"Vet. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1136\/vr.124.18.489","article-title":"Ageing of the unfertilised cow egg in vivo: How soon is fertility compromised?","volume":"124","author":"Hunter","year":"1989","journal-title":"Vet. Rec."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1111\/rda.13510","article-title":"Abortion in dairy cattle with advanced twin pregnancies: Incidence and timing","volume":"54","year":"2019","journal-title":"Reprod. Domest. Anim."},{"key":"ref_20","first-page":"121","article-title":"The dilemma of twin pregnancies in dairy cattle. A review of practical prospects","volume":"197","author":"Cabrera","year":"2017","journal-title":"Liv. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1016\/0093-691X(89)90473-1","article-title":"Twinning in dairy cattle: A study of risk factors and effects","volume":"32","author":"Nielen","year":"1989","journal-title":"Theriogenology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"39","DOI":"10.2527\/1999.77139x","article-title":"Effects of twinning on gestation length, retained placenta, and dystocia","volume":"77","author":"Echternkamp","year":"1999","journal-title":"J. Anim. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1016\/j.theriogenology.2012.07.027","article-title":"Effects of twinning on the subsequent reproductive performance and productive lifespan of high-producing dairy cows","volume":"78","author":"Ganau","year":"2012","journal-title":"Theriogenology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"260","DOI":"10.3168\/jds.S0022-0302(20)94273-X","article-title":"The relation of age of dam to observed fecundity in domesticated animals. I. Multiple births in cattle and sheep","volume":"3","author":"Jones","year":"1920","journal-title":"J. Dairy Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.2527\/1992.7082309x","article-title":"Fetal development in cattle with multiple ovulations","volume":"70","author":"Echternkamp","year":"1992","journal-title":"J. Anim. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1016\/j.theriogenology.2005.08.017","article-title":"The use of a deslorelin implant (GnRH agonist) during the late embryonic period to reduce pregnancy loss","volume":"65","author":"Bartolome","year":"2006","journal-title":"Theriogenology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3092","DOI":"10.3168\/jds.2008-1027","article-title":"Maintenance of pregnancy in dairy cattle after treatment with human chorionic gonadotropin or gonadotropin-releasing hormone","volume":"91","author":"Stevenson","year":"2008","journal-title":"J. Dairy Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1262\/jrd.2018-057","article-title":"The effects of a single or double GnRH dose on pregnancy survival in high producing dairy cows carrying singletons or twins","volume":"64","year":"2018","journal-title":"J. Reprod. Dev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1093\/humrep\/15.10.2228","article-title":"Embryo reduction of multifetal pregnancies following assisted reproduction treatment: A modification of the transvaginal ultrasound-guided technique","volume":"15","author":"Iberico","year":"2000","journal-title":"Hum. Reprod."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"406.e1","DOI":"10.1016\/j.ajog.2008.06.017","article-title":"Contemporary outcomes with the latest 1000 cases of multifetal pregnancy reduction (MPR)","volume":"199","author":"Stone","year":"2008","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.ejogrb.2018.09.008","article-title":"The outcome of the multifetal pregnancy reduction in a single centre: A report of 202 completed cases","volume":"230","author":"Yilanlioglu","year":"2018","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0378-4320(00)00112-3","article-title":"Twin reduction in the mare: Current options","volume":"60\u201361","author":"Macpherson","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.tvjl.2011.06.036","article-title":"Manual reduction of multiple embryos in the mare: The effect on subsequent pregnancy outcome","volume":"192","author":"Newcombe","year":"2012","journal-title":"Vet. J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"70","DOI":"10.2460\/javma.245.1.70","article-title":"Success rates of various techniques for reduction of twin pregnancy in mares","volume":"245","author":"Tan","year":"2014","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/S0007-1935(17)42154-3","article-title":"Observations on the frequency of fused foetal circulations in twin-bearing cattle","volume":"119","author":"Williams","year":"1963","journal-title":"Brit. Vet. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1262\/jrd.2012-046","article-title":"Manual rupture versus transvaginal ultrasound-guided aspiration of allanto-amniotic fluid in multiple pregnancies: A clinical approach to embryo reduction in dairy cattle","volume":"58","year":"2012","journal-title":"J. Reprod. Dev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"808","DOI":"10.3390\/ani3030808","article-title":"The European Market for Animal-Friendly Products in a Societal Context","volume":"3","author":"Ingenbleek","year":"2013","journal-title":"Animals"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Nalon, E., and Stevenson, P. (2019). Protection of Dairy Cattle in the EU: State of Play and Directions for Policymaking from a Legal and Animal Advocacy Perspective. Animals, 9.","DOI":"10.3390\/ani9121066"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Morton, R., Hebart, M.L., and Whittaker, A.L. (2020). Explaining the Gap Between the Ambitious Goals and Practical Reality of Animal Welfare Law Enforcement: A Review of the Enforcement Gap in Australia. Animals, 10.","DOI":"10.3390\/ani10030482"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"104908","DOI":"10.1016\/j.prevetmed.2020.104908","article-title":"Assessment of associations between transition diseases and reproductive performance of dairy cows using survival analysis and decision tree algorithms","volume":"176","author":"Probo","year":"2020","journal-title":"Prev. Vet. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1016\/j.theriogenology.2004.06.010","article-title":"Ovulation failure and double ovulation in dairy cattle: Risk factors and effects","volume":"63","author":"Fenech","year":"2005","journal-title":"Theriogenology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1530\/rep.1.00190","article-title":"Association between ovarian follicle development and pregnancy rates in dairy cows undergoing spontaneous oestrous cycles","volume":"127","author":"Bleach","year":"2004","journal-title":"Reproduction"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1016\/S0093-691X(99)00205-8","article-title":"Effect of milk production on the incidence of double ovulation in dairy cows","volume":"52","author":"Fricke","year":"1999","journal-title":"Theriogenology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2783","DOI":"10.3168\/jds.S0022-0302(05)72958-1","article-title":"Relationship between level of milk production and multiple ovulations in lactating dairy cows","volume":"88","author":"Lopez","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.theriogenology.2016.12.029","article-title":"Incidence of double ovulation during the early postpartum period in lactating dairy cows","volume":"91","author":"Kusaka","year":"2017","journal-title":"Theriogenology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.livsci.2019.09.004","article-title":"Preventing twin pregnancies in dairy cattle, turning the odds into reality","volume":"229","author":"Hunter","year":"2019","journal-title":"Liv. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1262\/jrd.2020-014","article-title":"Transfer of a single embryo versus drainage of subordinate follicles to prevent twin pregnancies in dairy cows. Why not both?","volume":"66","year":"2020","journal-title":"J. Reprod. Dev."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/11\/2006\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:27:26Z","timestamp":1760178446000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/11\/2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,31]]},"references-count":47,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11]]}},"alternative-id":["ani10112006"],"URL":"https:\/\/doi.org\/10.3390\/ani10112006","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,31]]}}}