{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:28:08Z","timestamp":1788848888489,"version":"build-2803163510"},"reference-count":81,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,11,9]],"date-time":"2020-11-09T00:00:00Z","timestamp":1604880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1180544"],"award-info":[{"award-number":["1180544"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Canine brucellosis caused by Brucella canis is a zoonotic disease that causes reproductive alterations in dogs, such as infertility, abortion, and epididymitis. This pathogen is especially prevalent in South America, and due to the lack of official control programs and the growing trend of adopting dogs it constitutes a public health risk that must be addressed. The aim of this study was to determine the prevalence of B. canis infection in kennel, shelter, and household dogs and to characterize the genomic properties of circulating strains, including ure and virB operons and omp25\/31 genes. Samples from 771 dogs were obtained, and the infection was detected by blood culture and\/or serology in 7.0% of the animals. The complete ure and virB operons and the omp25\/31 genes were detected. Interestingly, we found different single-nucleotide polymorphisms (SNPs) in some of the analyzed genes, which could mean a change in the fitness or virulence of these strains. This study provides further evidence about dogs as a source of B. canis strains that can infect people. This also highlights the need to implement official control programs, including the mandatory testing of dogs, especially stray dogs, before adoption.<\/jats:p>","DOI":"10.3390\/ani10112073","type":"journal-article","created":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T14:10:41Z","timestamp":1605017441000},"page":"2073","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Prevalence and Genomic Characterization of Brucella canis Strains Isolated from Kennels, Household, and Stray Dogs in Chile"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4288-898X","authenticated-orcid":false,"given":"Nicol\u00e1s","family":"Galarce","sequence":"first","affiliation":[{"name":"Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, 8820808 Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Escobar","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, 8820808 Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduard","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, 8820808 Santiago, Chile"},{"name":"Programa de Mag\u00edster en Ciencias Veterinarias, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, 8820808 Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Alvarado","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, 8820808 Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Peralta","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, 8820808 Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7619-046X","authenticated-orcid":false,"given":"Phillip","family":"Dettleff","sequence":"additional","affiliation":[{"name":"Laboratorio FAVET-INBIOGEN, Departamento de Fomento de la Producci\u00f3n Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, 8820808 Santiago, Chile"},{"name":"Escuela de Medicina Veterinaria, Facultad de Recursos Naturales y Medicina Veterinaria, Universidad Santo Tom\u00e1s, 3473620 Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Dorner","sequence":"additional","affiliation":[{"name":"Laboratorio FAVET-INBIOGEN, Departamento de Fomento de la Producci\u00f3n Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, 8820808 Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Laboratorio FAVET-INBIOGEN, Departamento de Fomento de la Producci\u00f3n Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, 8820808 Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Consuelo","family":"Borie","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, 8820808 Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Holst, B.S., L\u00f6fqvist, K., Ernholm, L., Eld, K., Cedersmyg, M., and Hallgren, G. 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