{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T04:57:07Z","timestamp":1780117027838,"version":"3.54.0"},"reference-count":56,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T00:00:00Z","timestamp":1605916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2018YFD0500206"],"award-info":[{"award-number":["2018YFD0500206"]}]},{"name":"Modern Agro-industry Technology Research System","award":["CARS-40"],"award-info":[{"award-number":["CARS-40"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772646"],"award-info":[{"award-number":["31772646"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972610"],"award-info":[{"award-number":["31972610"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Odour is one of the main environmental concerns in the laying hen industry and may also influence animal health and production performance. Previous studies showed that odours from the laying hen body are primarily produced from the microbial fermentation (breakdown) of organic materials in the caecum, and different laying hen species may have different odour production potentials. This study was conducted to evaluate the emissions of two primary odorous gases, ammonia (NH3) and hydrogen sulphide (H2S), from six different laying hen species (Hyline, Lohmann, Nongda, Jingfen, Xinghua and Zhusi). An in vitro fermentation technique was adopted in this study, which has been reported to be an appropriate method for simulating gas production from the microbial fermentation of organic materials in the caecum. The results of this study show that Jingfen produced the greatest volume of gas after 12 h of fermentation (p &lt; 0.05). Hyline had the highest, while Lohmann had the lowest, total NH3 emissions (p &lt; 0.05). The total H2S emissions of Zhusi and Hyline were higher than those of Lohmann, Jingfen and Xinghua (p &lt; 0.05), while Xinghua exhibited the lowest total H2S emissions (p &lt; 0.05). Of the six laying hen species, Xinghua was identified as the best species because it produced the lowest total amount of NH3 + H2S (39.94 \u00b5g). The results for the biochemical indicators showed that the concentration of volatile fatty acids (VFAs) from Zhusi was higher than that for the other five species, while the pH in Zhusi was lower (p &lt; 0.01), and the concentrations of ammonium nitrogen (NH4+), uric acid and urea in Xinghua were lower than those in the other species (p &lt; 0.01). Hyline had the highest change in SO42\u2212 concentration during the fermentation processes (p &lt; 0.05). In addition, the results of the correlation analysis suggested that NH3 emission is positively related to urease activities but is not significantly related to the ureC gene number. Furthermore, H2S emission was observed to be significantly related to the reduction of SO42\u2212 but showed no connection with the aprA gene number. Overall, our findings provide a reference for future feeding programmes attempting to reduce odour pollution in the laying hen industry.<\/jats:p>","DOI":"10.3390\/ani10112172","type":"journal-article","created":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T08:18:23Z","timestamp":1606119503000},"page":"2172","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Effects of Different Laying Hen Species on Odour Emissions"],"prefix":"10.3390","volume":"10","author":[{"given":"Dongdong","family":"Lu","sequence":"first","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiandui","family":"Mi","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinbao","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6024-0856","authenticated-orcid":false,"given":"Juanboo","family":"Liang","sequence":"additional","affiliation":[{"name":"Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xindi","family":"Liao","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,21]]},"reference":[{"key":"ref_1","first-page":"280","article-title":"Analysis on production efficiency of laying hens in China\u2014Based on the survey data of five provinces","volume":"9","author":"Wu","year":"2019","journal-title":"J. Agric. Sci. Camb."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Wi, J., Lee, S., Kim, E., Lee, M., Koziel, J., and Ahn, H. (2019). Evaluation of semi-continuous pit manure recharge system performance on mitigation of ammonia and hydrogen sulfide emissions from a swine finishing barn. Atmosphere, 10.","DOI":"10.3390\/atmos10040170"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.ijheh.2019.09.003","article-title":"Estimated time-varying exposures to air emissions from animal feeding operations and childhood asthma","volume":"223","author":"Loftus","year":"2020","journal-title":"Int. J. Hyg. Environ. Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s40572-014-0038-4","article-title":"Recent developments in the quantification and regulation of air emissions from animal feeding operations","volume":"2","author":"Heinzen","year":"2015","journal-title":"Curr. Environ. Health Rep."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Liu, Z.F., Liu, Y., Murphy, J., and Maghirang, R. (2017). Ammonia and methane emission factors from cattle operations expressed as losses of dietary nutrients or energy. Agriculture, 7.","DOI":"10.3390\/agriculture7030016"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.13031\/jash.12990","article-title":"Occupational exposure risk for swine workers in confined housing facilities","volume":"25","author":"Alvarado","year":"2019","journal-title":"J. Agric. Saf. Health"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.atmosenv.2011.10.021","article-title":"Ammonia, hydrogen sulfide, carbon dioxide and particulate matter emissions from California high-rise layer houses","volume":"46","author":"Lin","year":"2012","journal-title":"Atmos. Environ."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"15269","DOI":"10.1007\/s11356-018-2018-y","article-title":"Ammonia production in poultry houses can affect health of humans, birds, and the environment\u2014Techniques for its reduction during poultry production","volume":"25","author":"Naseem","year":"2018","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.scitotenv.2018.12.375","article-title":"Ammonia induces Treg\/Th1 imbalance with triggered NF-\u03baB pathway leading to chicken respiratory inflammation response","volume":"659","author":"Shi","year":"2019","journal-title":"Sci. Total. Environ."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.wasman.2019.05.019","article-title":"Mitigating ammonia emissions from typical broiler and layer manure management\u2013A system analysis","volume":"93","author":"Wang","year":"2019","journal-title":"Waste Manag."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.niox.2017.09.011","article-title":"Environmental toxicology of hydrogen sulfide","volume":"71","author":"Pearce","year":"2017","journal-title":"Nitric Oxide"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.ecoenv.2018.08.029","article-title":"Hydrogen sulfide inhalation-induced immune damage is involved in oxidative stress, inflammation, apoptosis and the Th1\/Th2 imbalance in broiler bursa of Fabricius","volume":"164","author":"Hu","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1007\/s12011-016-0752-x","article-title":"Role of hydrogen sulfide on autophagy in liver injuries induced by selenium deficiency in chickens","volume":"175","author":"Wang","year":"2017","journal-title":"Bio. Trace Elem. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"8061823","DOI":"10.1155\/2019\/8061823","article-title":"Hydrogen sulfide gas exposure induces necroptosis and promotes inflammation through the MAPK\/NF-\u03baB pathway in broiler spleen","volume":"2019","author":"Chi","year":"2019","journal-title":"Oxidative. Med. Cell. Longev."},{"key":"ref_15","unstructured":"Chou, C.H., Ogden, J.M., Pohl, H.R., Scinicariello, F., Ingerman, L., Barber, L., and Citra, M.J. (2016). Toxicological Profile for Hydrogen Sulfide and Carbonyl Sulfide. Agency for Toxic Substances and Disease Registry. Division of Toxicology and Human Health Sciences, Environmental Toxicology Branch."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Hofmann, T., Schmucker, S.S., Bessei, W., Grashorn, M., and Stefanski, V. (2020). Impact of housing environment on the immune system in chickens: A review. Animals, 10.","DOI":"10.3390\/ani10071138"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"G159","DOI":"10.1152\/ajpgi.00319.2017","article-title":"Protein fermentation in the gut implications for intestinal dysfunction in humans, pigs, and poultry","volume":"315","author":"Gilbert","year":"2018","journal-title":"Am. J. Physiol.-Gastrointest. Live Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"16272","DOI":"10.1007\/s11356-016-6777-z","article-title":"Sodium butyrate mitigates in vitro ammonia generation in cecal content of laying hens","volume":"23","author":"Wang","year":"2016","journal-title":"Environ. Sci. Pollut. R."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.mineng.2014.07.010","article-title":"Organic wastes as carbon sources to promote sulfate reducing bacterial activity for biological remediation of acid mine drainage","volume":"69","author":"Zhang","year":"2014","journal-title":"Miner. Eng."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Kushkevych, I., Le\u0161\u010danov\u00e1, O., Dordevi\u0107, D., Jan\u010d\u00edkov\u00e1, S., Ho\u0161ek, J., V\u00edt\u011bzov\u00e1, M., Bu\u0148kov\u00e1, L., and Drago, L. (2019). The sulfate-reducing microbial communities and meta-analysis of their occurrence during diseases of small\u2013large intestine axis. J. Clin. Med., 8.","DOI":"10.3390\/jcm8101656"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4184","DOI":"10.1128\/AEM.00606-14","article-title":"Effects of pH and lactate on hydrogen sulfide production by oral Veillonella spp","volume":"80","author":"Washio","year":"2014","journal-title":"Appl. Environ. Microb."},{"key":"ref_22","first-page":"1176934317700863","article-title":"Free-Living Enterobacterium Pragia fontium 24613: Complete genome sequence and metabolic profiling","volume":"13","year":"2017","journal-title":"Evol. Bioinform."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.cell.2014.09.053","article-title":"Human Genetics Shape the Gut Microbiome","volume":"159","author":"Goodrich","year":"2014","journal-title":"Cell"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s40168-017-0384-1","article-title":"Host contributes to longitudinal diversity of fecal microbiota in swine selected for lean growth","volume":"6","author":"Lu","year":"2018","journal-title":"Microbiome"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.13031\/2013.41510","article-title":"Effects of laying-hen strain on manure properties and ammonia emission","volume":"55","author":"Rahman","year":"2012","journal-title":"Trans. ASABE"},{"key":"ref_26","unstructured":"NRC (1994). Nutrient Requirements of Poultry, National Academy Press. [9th ed.]."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"56","DOI":"10.3382\/ps\/pey366","article-title":"Metagenomics analysis reveals significant modulation of cecal microbiota of broilers fed palm kernel expeller diets","volume":"98","author":"Chen","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_28","first-page":"7","article-title":"Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid","volume":"28","author":"Menke","year":"1988","journal-title":"Anim. Res. Dev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"132","DOI":"10.4081\/ijas.2015.3762","article-title":"Prebiotics mitigate in vitro sulfur-containing odour generation in caecal content of pigs","volume":"14","author":"Deng","year":"2015","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_30","unstructured":"Guan, S.Y. (1986). Soil Enzyme and Its Research Methods, Agriculture Press."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1017\/S175173110999070X","article-title":"Developing speciesing schemes to assist mitigation of greenhouse gas emissions","volume":"4","author":"Wall","year":"2010","journal-title":"Animal"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"106748","DOI":"10.1016\/j.agee.2019.106748","article-title":"Can dietary manipulations improve the productivity of pigs with lower environmental and economic cost? A global meta-analysis","volume":"289","author":"Wang","year":"2020","journal-title":"Agric. Ecosyst. Environ."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"20888","DOI":"10.1073\/pnas.1308149110","article-title":"Biomass use, production, feed efficiencies, and greenhouse gas emissions from global livestock systems","volume":"110","author":"Herrero","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.atmosenv.2017.12.018","article-title":"Emission factors of greenhouse gases from layer and broiler barns in Cameroon","volume":"176","author":"Ngwabie","year":"2018","journal-title":"Atmos. Environ."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.anifeedsci.2015.08.001","article-title":"Organic egg production. I: Effects of different dietary protein contents and forage material on organic egg production, nitrogen and mineral retention and total tract digestibility of nutrients of two hen genotypes","volume":"209","author":"Steenfeldt","year":"2015","journal-title":"Animal Feed Sci. Technol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"37684","DOI":"10.1007\/s11356-020-09858-y","article-title":"Determination of ammonia and hydrogen sulfide emissions from a commercial dairy farm with an exercise yard and the health-related impact for residents","volume":"27","author":"Wu","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6808","DOI":"10.3382\/ps\/pez412","article-title":"The effects of betaine supplementation in diets containing different levels of crude protein and methionine on the growth performance, blood components, total tract nutrient digestibility, excreta noxious gas emission, and meat quality of the broiler chickens","volume":"98","author":"Park","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1093\/ajcn\/72.6.1488","article-title":"Contribution of dietary protein to sulfide production in the large intestine: An in vitro and a controlled feeding study in humans","volume":"72","author":"Magee","year":"2000","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s40781-017-0153-5","article-title":"Effects of amino acid composition in pig diet on odorous compounds and microbial characteristics of swine excreta","volume":"59","author":"Recharla","year":"2017","journal-title":"J. Anim. Sci. Technol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3977","DOI":"10.3382\/ps\/pez138","article-title":"Screening of single or combined administration of 9 probiotics to reduce ammonia emissions from laying hens","volume":"98","author":"Mi","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_41","first-page":"574","article-title":"Potential to mitigate ammonia emission from slurry by increasing dietary fermentable fiber through inclusion of tropical byproducts in practical diets for growing pigs","volume":"32","author":"Nguyen","year":"2019","journal-title":"Asian Austral. J. Anim."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1079\/NRR200592","article-title":"Odour from animal production facilities: Its relationship to diet","volume":"18","author":"Le","year":"2005","journal-title":"Nutr. Res. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.livsci.2010.06.140","article-title":"Dietary supplementation with chitosan at high and low crude protein concentrations promotes Enterobacteriaceae in the cecum and colon and increases manure odour emissions from finisher boars","volume":"134","author":"Lynch","year":"2010","journal-title":"Livest. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"937","DOI":"10.3389\/fphys.2019.00937","article-title":"Probiotic properties of Lactobacillus paracasei subsp. paracasei L1 and its growth performance-promotion in chicken by improving the intestinal microflora","volume":"10","author":"Xu","year":"2019","journal-title":"Front. Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4465","DOI":"10.3382\/ps\/pez282","article-title":"Effect of dietary probiotics and prebiotics on the performance of broiler chickens","volume":"98","author":"Ragheb","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Williams, B.L., Hornig, M., Parekh, T., and Lipkin, W.I. (2012). Application of novel PCR-based methods for detection, quantitation, and phylogenetic characterization of Sutterella species in intestinal biopsy samples from children with autism and gastrointestinal disturbances. Mbio, 3.","DOI":"10.1128\/mBio.00261-11"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/s40168-018-0501-9","article-title":"Microbial diversity and community composition of caecal microbiota in commercial and indigenous Indian chickens determined using 16s rDNA amplicon sequencing","volume":"6","author":"Pandit","year":"2018","journal-title":"Microbiome"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.3389\/fmicb.2016.01006","article-title":"Insights into abundant rumen ureolytic bacterial community using rumen simulation system","volume":"7","author":"Jin","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"21460","DOI":"10.1002\/jcp.28870","article-title":"Potential antigen candidates for subunit vaccine development against Helicobacter pylori infection","volume":"234","author":"Keikha","year":"2019","journal-title":"J. Cell. Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1093\/gbe\/evx112","article-title":"Comparative genomics of all three campylobacter sputorum biovars and a novel cattle-associated C. sputorum clade","volume":"9","author":"Miller","year":"2017","journal-title":"Genome Biol. Evol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"4925","DOI":"10.1128\/AEM.68.10.4925-4931.2002","article-title":"Ammonia production by ruminal microorganisms and enumeration, isolation, and characterization of bacteria capable of growth on peptides and amino acids from the sheep rumen","volume":"68","author":"Eschenlauer","year":"2002","journal-title":"Appl. Environ. Microb."},{"key":"ref_52","first-page":"R214","article-title":"Glutamine synthetase in avian muscle contributes to a positive myogenic response to ammonia compared with mammalian muscle","volume":"317","author":"Stern","year":"2019","journal-title":"Am. J. Physiol.-Regul. I."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1111\/jpn.13080","article-title":"Differential ammonia metabolism and toxicity between avian and mammalian species, and effect of ammonia on skeletal muscle: A comparative review","volume":"103","author":"Stern","year":"2019","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1007\/s00044-017-2067-7","article-title":"Activity of ring-substituted 8-hydroxyquinoline-2-carboxanilides against intestinal sulfate-reducing bacteria Desulfovibrio piger","volume":"27","author":"Kushkevych","year":"2018","journal-title":"Med. Chem. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3478","DOI":"10.1099\/mic.0.2007\/008250-0","article-title":"Molecular analysis of the distribution and phylogeny of dissimilatory adenosine-5\u2019-phosphosulfate reductase-encoding genes (aprBA) among sulfur-oxidizing prokaryotes","volume":"15","author":"Meyer","year":"2007","journal-title":"Microbiology"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"4223","DOI":"10.1093\/jxb\/erz118","article-title":"Thiol redox-regulation for efficient adjustment of sulfur metabolism in acclimation to abiotic stress","volume":"70","author":"Telman","year":"2019","journal-title":"J. Exp. Bot."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/11\/2172\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:35:19Z","timestamp":1760178919000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/11\/2172"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,21]]},"references-count":56,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11]]}},"alternative-id":["ani10112172"],"URL":"https:\/\/doi.org\/10.3390\/ani10112172","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,21]]}}}