{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:32:36Z","timestamp":1787027556225,"version":"build-2736575974"},"reference-count":59,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T00:00:00Z","timestamp":1607040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["CGL2015-68110-R"],"award-info":[{"award-number":["CGL2015-68110-R"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["FJCI-2016-30990"],"award-info":[{"award-number":["FJCI-2016-30990"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Organismo Aut\u00f3nomo de Parques Nacionales de Espa\u00f1a","award":["1541S\/2015"],"award-info":[{"award-number":["1541S\/2015"]}]},{"DOI":"10.13039\/501100006366","name":"Minist\u00e9rio da Educa\u00e7\u00e3o","doi-asserted-by":"publisher","award":["PDSE 88881.187275\/2018-01"],"award-info":[{"award-number":["PDSE 88881.187275\/2018-01"]}],"id":[{"id":"10.13039\/501100006366","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["CGL2015-68110-R"],"award-info":[{"award-number":["CGL2015-68110-R"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Isotopic stable analysis (SIA) is a powerful tool in the assessment of different types of ecological and physiological studies. For that, different preservation methods for sampled materials are commonly used prior to isotopic analysis. The effects of various preservation methods (freezing, ethanol and formaldehyde) were analyzed for C:N, and \u03b413C and \u03b415N signals on a variety of tissues including dorsal fins (three seahorse and two pipefish species), seahorse newborns (three seahorses species), and prey (copepods and different stages of Artemia) commonly used to feed the fishes under rearing conditions. The aims of the study were: (i) to evaluate isotopic effects of chemical preservation methods across different types of organisms and tissues, using frozen samples as controls, and (ii) to construct the first conversion models available in syngnathid fishes. The chemical preservation in ethanol and, to a lesser extent, in formaldehyde significantly affected \u03b413C values, whereas the effects on \u03b415N signatures were negligible. Due to their low lipid content, the isotopic signals in fish fins was almost unaffected, supporting the suitability of dorsal fins as the most convenient material in isotopic studies on vulnerable species such as syngnathids. The regression equations provided resulted convenient for the successful conversion of \u03b413C between preservation treatments. Our results indicate that the normalization of \u03b415N signatures in preserved samples is unnecessary. The conversion models should be applicable in isotopic field studies, laboratory experiments, and specimens of historical collections.<\/jats:p>","DOI":"10.3390\/ani10122301","type":"journal-article","created":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T21:37:42Z","timestamp":1607377062000},"page":"2301","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of Tissue Preservation on Carbon and Nitrogen Stable Isotope Signatures in Syngnathid Fishes and Prey"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1217-5716","authenticated-orcid":false,"given":"Miquel","family":"Planas","sequence":"first","affiliation":[{"name":"Institute of Marine Research (CSIC), 36208 Vigo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Paltrinieri","sequence":"additional","affiliation":[{"name":"Institute of Marine Research (CSIC), 36208 Vigo, Spain"},{"name":"Dipartimento di Scienze della Vita e dell\u2019Ambiente, Universit\u00e0 Politecnica delle Marche, 60131 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2037-9890","authenticated-orcid":false,"given":"Mario Davi Dias","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Institute of Marine Research (CSIC), 36208 Vigo, Spain"},{"name":"Laborat\u00f3rio de Piscicultura Estuarina e Marinha, Instituto de Oceanografia, Universidade Federal do Rio Grande\u2014FURG, Rio Grande, RS 96203-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1032-2786","authenticated-orcid":false,"given":"Jorge","family":"Hern\u00e1ndez-Urcera","sequence":"additional","affiliation":[{"name":"Institute of Marine Research (CSIC), 36208 Vigo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1890\/0012-9658(2002)083[0703:USITET]2.0.CO;2","article-title":"Using stable isotopes to estimate trophic position: Models, methods, and assumptions","volume":"83","author":"Post","year":"2002","journal-title":"Ecology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1146\/annurev.es.18.110187.001453","article-title":"Stable isotopes in ecosystem studies","volume":"18","author":"Peterson","year":"1987","journal-title":"Annu. 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