{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T01:58:01Z","timestamp":1767664681423,"version":"build-2065373602"},"reference-count":29,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T00:00:00Z","timestamp":1608163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Renal hyperparathyroidism (RHPT) is one of the main complications in dogs affected with Chronic Kidney Disease (CKD). The measurement of serum parathyroid hormone (PTH) could be of clinical utility for the disease\u2019s treatment and follow-up; however, PTH is not routinely determined due to limited available methods, often not fully validated in dogs. The aims of this study were the analytical validation of an immunoenzymatic method for the measurement of PTH in canine serum and the analysis of preliminary association of the obtained results with renal function. Twenty-six samples obtained from dogs healthy or affected with CKD were analysed. PTH was measured using a two-site immunoenzymometric human assay (ST AIA-PACK\u00ae Intact PTH, Tosoh Bioscience). The analytical validation protocol evaluated the assay precision and accuracy. Also, the PTH\u2019s storage stability at 20 \u00b0C, 4 \u00b0C and \u221220 \u00b0C was assessed. Clinical validation was performed by comparing PTH values with creatinine, phosphorus and International Renal Interest Society (IRIS) stage. The method showed optimal precision and accuracy, whereas stability was adequate up to 4 h at 20 \u00b0C, 24 h at 4 \u00b0C and 6 months at \u221220 \u00b0C. PTH was positively associated with creatinine, phosphorus and IRIS stage. The investigated method was thus successfully validated in dogs, allowing its use for clinical purpose.<\/jats:p>","DOI":"10.3390\/ani10122411","type":"journal-article","created":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T10:42:47Z","timestamp":1608201767000},"page":"2411","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Analytical and Clinical Validation of a New Immunoenzymatic Method for the Measurement of Canine Parathyroid Hormone"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2726-6090","authenticated-orcid":false,"given":"Jari","family":"Zambarbieri","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, University of Milan, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filippo","family":"Tagliasacchi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Milan, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9708-5148","authenticated-orcid":false,"given":"Pierangelo","family":"Moretti","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Milan, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8611-8944","authenticated-orcid":false,"given":"Alessia","family":"Giordano","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Milan, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2316-1929","authenticated-orcid":false,"given":"Paola","family":"Scarpa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Milan, Via dell\u2019Universit\u00e0 6, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/0378-1119(94)00912-C","article-title":"Sequences of the cDNA encoding canine parathyroid hormone-related protein and parathyroid hormone","volume":"160","author":"Rosol","year":"1995","journal-title":"Gene"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/j.cvsm.2016.06.003","article-title":"Update on mineral and bone disorders in chronic kidney disease","volume":"46","author":"Foster","year":"2016","journal-title":"Vet. Clin. Small Anim."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1111\/j.1939-1676.2009.0415.x","article-title":"Calcium and phosphorus homeostasis in dogs with spontaneous chronic kidney disease at different stages of severity","volume":"24","author":"Cortadellas","year":"2010","journal-title":"J. Vet. Intern. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1111\/jvim.14653","article-title":"Association of vitamin D metabolites with parathyroid hormone, fibroblast growth factor-23, calcium, and phosphorus in dogs with various stages of chronic kidney disease","volume":"31","author":"Parker","year":"2017","journal-title":"J. Vet. Intern. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/vec.12036","article-title":"Calcitriol, calcidiol, parathyroid hormone, and fibroblast growth factor-23 interactions in chronic kidney disease","volume":"23","author":"Nagode","year":"2013","journal-title":"J. Vet. Emerg. Crit. Care"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1093\/ndt\/gfg104","article-title":"Dynamics of secretion and metabolism of PTH during hypo- and hypercalcemia in the dog as determined by the \u2018intact\u2019 and \u2018whole\u2019 PTH assays","volume":"18","author":"Estepa","year":"2003","journal-title":"Nephrol. Dial. Transplant."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1111\/jvim.14707","article-title":"Fibroblast growth factor-23 concentration in dogs with chronic kidney disease","volume":"31","author":"Harjes","year":"2017","journal-title":"J. Vet. Intern. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.rvsc.2017.06.013","article-title":"Serum fibroblast growth factor 23 concentrations in dogs with chronic kidney disease","volume":"114","author":"Dittmer","year":"2017","journal-title":"Res. Vet. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1210\/er.2018-00220","article-title":"Clinical guidelines and PTH measurement: Does assay generation matter?","volume":"40","author":"Smit","year":"2019","journal-title":"Endocr. Rev."},{"key":"ref_10","first-page":"1123","article-title":"Human-parathormone assay for use in dogs: Validation, sample handling studies, and parathyroid function testing","volume":"50","author":"Torrance","year":"1989","journal-title":"Am. J. Vet. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1111\/j.1939-1676.1989.tb03084.x","article-title":"Intact parathyroid hormone assay and total calcium concentration in the diagnosis of disorders of calcium metabolism in dogs","volume":"3","author":"Torrance","year":"1989","journal-title":"J. Vet. Intern. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1136\/vr.157.12.344","article-title":"Diurnal variations in the plasma concentration of parathyroid hormone in dogs","volume":"157","author":"Estepa","year":"2005","journal-title":"Vet. Rec."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4581","DOI":"10.21897\/rmvz.61","article-title":"Reference values of parathyroid hormone and vitamin D hormone by chemiluminescent automated assay","volume":"20","author":"Martiarena","year":"2015","journal-title":"Rev. MVZ C\u00f3rdoba"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.2460\/ajvr.2003.64.1161","article-title":"Serum concentrations of 1,25-dihydroxycholecalciferol and 25-hydroxycholecalciferol in clinically normal dogs and dogs with acute and chronic renal failure","volume":"64","author":"Gerber","year":"2003","journal-title":"Am. J. Vet. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1111\/vco.12101","article-title":"Serum 25-hydroxyvitamin D concentrations in dogs \u2013 correlation with health and cancer risk","volume":"14","author":"Selting","year":"2016","journal-title":"Vet. Comp. Oncol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1111\/jsap.13006","article-title":"A comparison of canine whole and intact parathyroid hormone concentrations as measured by different assays","volume":"60","author":"Mooney","year":"2019","journal-title":"J. Small Anim. Pract."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1111\/jvim.15322","article-title":"Factor associated with survival in dogs with chronic kidney disease","volume":"32","author":"Rudinsky","year":"2018","journal-title":"J. Vet. Intern. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1111\/jvim.15255","article-title":"25-Hydroxyvitamin D concentrations in dogs with naturally acquired blastomycosis","volume":"32","author":"McMichael","year":"2018","journal-title":"J. Vet. Intern. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1111\/j.1532-950X.2008.00457.x","article-title":"Validation of a rapid parathyroid hormone assay and intraoperative measurement of parathyroid hormone in dogs with benign naturally occurring primary hyperparathyroidism","volume":"38","author":"Ham","year":"2009","journal-title":"Vet. Surg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1111\/j.1751-0813.2012.00918.x","article-title":"Intraoperative parathyroid hormone concentration to confirm removal of hypersecretory parathyroid tissue and time to postoperative normocalcemia in ine dogs with primary hyperparathyroidism","volume":"90","author":"Graham","year":"2012","journal-title":"Aust. Vet. J."},{"key":"ref_21","first-page":"S49","article-title":"Limit of blank, limit of detection and limit of quantitation","volume":"29","author":"Armbruster","year":"2008","journal-title":"Clin. Biochem. Rev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/vcp.12150","article-title":"Validation of a commercially available automated canine-specific immunoturbidimetric method for measuring canine C-reactive protein","volume":"43","author":"Hagman","year":"2014","journal-title":"Vet. Clin. Pathol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1590\/S0102-09352006000400006","article-title":"Serum concentration of intact parathormone in dogs with chronic renal failure","volume":"58","author":"Lazaretti","year":"2006","journal-title":"Arq. Bras. Med. Vet. Zootec"},{"key":"ref_24","first-page":"223","article-title":"Intraindividual variation in serum thyroid hormones, parathyroid hormone and insulin-like growth factor-1","volume":"45","author":"Wijeratne","year":"2008","journal-title":"Ann. Clin. Biochem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1515\/CCLM.2008.275","article-title":"Analytical quality goals for parathyroid hormone based on biological variation","volume":"46","author":"Viljoen","year":"2008","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_26","unstructured":"(2020, September 19). Guideline on Bioanalytical Method Validation. Available online: https:\/\/www.ema.europa.eu\/en\/documents\/scientific-guideline\/guideline-bioanalytical-method-validation_en.pdf."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.1515\/cclm-2013-0315","article-title":"Sampling and storage conditions influencing the measurement of parathyroid hormone in blood samples: A systematic review","volume":"51","author":"Hanon","year":"2013","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_28","unstructured":"Wild, D. (2013). Principles of competitive and immunometric assays (including ELISA). The Immunoassay Handbook, Elsevier. [4th ed.]."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5496710","DOI":"10.1155\/2019\/5496710","article-title":"Parathyroid hormone measurement in chronic kidney disease: From basics to clinical implications","volume":"2019","author":"Kritmetapak","year":"2019","journal-title":"Int. J. Nephrol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/12\/2411\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:46:09Z","timestamp":1760179569000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/10\/12\/2411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,17]]},"references-count":29,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["ani10122411"],"URL":"https:\/\/doi.org\/10.3390\/ani10122411","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2020,12,17]]}}}