{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:28:05Z","timestamp":1791606485516,"version":"4.3.4"},"reference-count":32,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T00:00:00Z","timestamp":1608768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Polish National Centre for Research and Development (NCBiR),","award":["NCBiR PBS3\/B8\/16\/2015."],"award-info":[{"award-number":["NCBiR PBS3\/B8\/16\/2015."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The aim of the study was to compare the morphology and developmental potential of oocytes obtained from adult and prepubertal domestic cats (Felis catus) and wild cats (Lynx lynx, Leptailurus serval, Felis manul, Panthera tigris altaica). The average number of oocytes obtained from an adult domestic cat was 23 \u00b1 11, which was significantly lower than from kittens (43 \u00b1 29). A similar number of oocytes was derived from adult Pallas\u2019s cats (28 \u00b1 8), and serval (30). The lowest number of oocytes was collected from the lynx (5 \u00b1 3). No oocytes were obtained from newborn Amur tiger while in the case of older domestic and Pallas\u2019s cat and lynx kittens (1\u20133 months) 43, 48 and 41 oocytes were collected, respectively. Significant differences (p &lt; 0.001) were observed between the number of oocytes with dark cytoplasm from adult and prepubertal animals of all analyzed species. The diameter of oocytes from adult and prepubertal animals was similar in all species, and was on average 161 \u00b1 4 \u00b5m for oocytes with dark cytoplasm and 150 \u00b1 18 \u00b5m for oocytes with light cytoplasm. In all species, oocytes with light cytoplasm were significantly smaller (p &lt; 0.05) than dark ones, and their population was more diverse. Results of in vitro maturation of the domestic and wild cat\u2032s oocytes obtained from adult and prepubertal females were similar (47\u201352%). The cleavage rate after in vitro fertilization (IVF) was lower for prepubertal than adult domestic cats (42 vs. 51%; p &lt; 0.05%). Moreover, we observed differences in the quantity (28 vs. 39%; p &lt; 0.05) and quality of blastocysts and even greater problems with hatching blastocysts from prepubertal kittens (8 vs. 19%; p &lt; 0.001). More blastomeres were detected in blastocysts of adult cats. They also demonstrated significantly higher number of inner cell mass (ICM) (p &lt; 0.001) and higher number of trophoblast cells (TE) (p &lt; 0.05).<\/jats:p>","DOI":"10.3390\/ani11010020","type":"journal-article","created":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T09:02:44Z","timestamp":1608800564000},"page":"20","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Comparison of the Morphology and Developmental Potential of Oocytes Obtained from Prepubertal and Adult Domestic and Wild Cats"],"prefix":"10.3390","volume":"11","author":[{"given":"Joanna","family":"Kochan","sequence":"first","affiliation":[{"name":"Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture, Mickiewicza 24\/28, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Nowak","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture, Mickiewicza 24\/28, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wies\u0142awa","family":"M\u0142odawska","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture, Mickiewicza 24\/28, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5708-8141","authenticated-orcid":false,"given":"Sylwia","family":"Prochowska","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Clinic of Farm Animals, University of Environmental Science, Grundwaldzki Square 49, 50-357 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9131-3552","authenticated-orcid":false,"given":"Agnieszka","family":"Partyka","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Clinic of Farm Animals, University of Environmental Science, Grundwaldzki Square 49, 50-357 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f3zef","family":"Skotnicki","sequence":"additional","affiliation":[{"name":"Foundation Municipal Park and the Zoological Garden in Cracow, ul. Kasy Oszcz\u0119dno\u015bci Miasta Krakowa 14, 30-232 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wojciech","family":"Ni\u017ca\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Clinic of Farm Animals, University of Environmental Science, Grundwaldzki Square 49, 50-357 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"457","DOI":"10.2478\/jvetres-2019-0043","article-title":"ARTs in wild felid conservation programmes in Poland and in the world","volume":"63","author":"Kochan","year":"2019","journal-title":"J. Vet. 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