{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T10:45:48Z","timestamp":1786358748066,"version":"build-2736575974"},"reference-count":40,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T00:00:00Z","timestamp":1608768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda&amp;#x00E7;&amp;#x00E3;o de Amparo &amp;#x00E0; Pesquisa do Estado de S&amp;#x00E3;o Paulo","award":["2016\/02103-0"],"award-info":[{"award-number":["2016\/02103-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>We evaluated the clinical aspects and feeding behavior of cattle with subacute ruminal acidosis (SARA) caused by short-chain fatty acids (SCFAs). Ten healthy Nelore heifers were subjected to an adjusted SARA induction protocol using citrus pulp (CP). Clinical examinations were performed at baseline and at 3, 6, 9, 12, 15, 18, and 24 h intervals after induction, with ruminal fluid, blood, and feces sampling. The animals\u2019 feeding behavior was evaluated on, before, and for 3 days after SARA by observing the animals every 5 min for 24 h. The dry matter intake (DMI) was recorded daily. The ruminal pH during SARA was always lower than baseline, with an acidotic duration of 547 \u00b1 215 min, a minimum pH of 5.38 \u00b1 0.16, and an average pH of 5.62 \u00b1 0.1. SARA was mainly caused by SCFAs (maximum 118.4 \u00b1 9.3 mmol\/L), with the production of l-lactic acids (7.17 mmol\/L) and d-lactic acids (0.56 mmol\/L) 6 h after the experiment began. The DMI was reduced by 66% and 48% on days 1 and 2, respectively, and returned to normal levels on day 3. SARA caused a reduction in feed intake and rumination time, as well as an increase in the time spent in decubitus on days 1 and 2. These results were influenced by the ruminal pH, ruminal movement, and osmolarity. Furthermore, SARA caused different degrees of depression, which became more pronounced with higher ruminal lactic acid concentrations.<\/jats:p>","DOI":"10.3390\/ani11010021","type":"journal-article","created":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T22:56:45Z","timestamp":1608850605000},"page":"21","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Subacute Ruminal Acidosis in Zebu Cattle: Clinical and Behavioral Aspects"],"prefix":"10.3390","volume":"11","author":[{"given":"Natalia Sato","family":"Minami","sequence":"first","affiliation":[{"name":"Department of Clinical Science, College of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo (FMVZ\/USP), 05509-270 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rejane Santos","family":"Sousa","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, College of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo (FMVZ\/USP), 05509-270 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Leonardo Costa","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, College of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo (FMVZ\/USP), 05509-270 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mailson Rennan Borges","family":"Dias","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, College of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo (FMVZ\/USP), 05509-270 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e9bora Aparecida","family":"Cassiano","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, College of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo (FMVZ\/USP), 05509-270 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara Satsuki","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, College of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo (FMVZ\/USP), 05509-270 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6742-3652","authenticated-orcid":false,"given":"Antonio Humberto Hamad","family":"Minervino","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Health, LARSANA, Federal University of Western Par\u00e1, UFOPA, 68040-255 Santar\u00e9m, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico Lippi","family":"Ortolani","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, College of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo (FMVZ\/USP), 05509-270 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3427","DOI":"10.2527\/jas.2009-1880","article-title":"A snapshot of management practices and nutritional recommendations used by feedlot nutritionists in Brazil","volume":"87","author":"Millen","year":"2009","journal-title":"J. Anim. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.2527\/1998.761275x","article-title":"Acidosis in cattle: A review","volume":"76","author":"Owens","year":"1998","journal-title":"J. Anim. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3721","DOI":"10.5433\/1679-0359.2015v36n6p3721","article-title":"Clinical evaluation of unadapted sheep submited to sudden intake of melon with high levels of sugar","volume":"36","author":"Oliveira","year":"2015","journal-title":"Semin. Agrar."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.cvfa.2007.04.002","article-title":"Acidosis in Feedlot Cattle","volume":"23","author":"Nagaraja","year":"2007","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_5","first-page":"421","article-title":"Comparative study of the citrus pulp to cause acute rumen lactic acidosis in cattle","volume":"45","author":"Minervino","year":"2008","journal-title":"Braz. J. Vet. Res. Anim. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2616","DOI":"10.3168\/jds.S0022-0302(86)80709-3","article-title":"In Vitro Fermentation of Sugars, Grains, and By-Product Feeds in Relation to Initiation of Ruminal Lactate Production","volume":"69","author":"Cullen","year":"1986","journal-title":"J. Dairy Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1046\/j.1439-0442.2003.00569.x","article-title":"Subacute ruminal acidosis (SARA): A review","volume":"50","author":"Kleen","year":"2003","journal-title":"J. Vet. Med. Ser. A Physiol. Pathol. Clin. Med."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Fleming, A., Garrett, K., Froehlich, K., Beck, M., Bryant, R.H., Edwards, G., and Gregorini, P. (2020). Supplementation of Spring Pasture with Harvested Fodder Beet Bulb Alters Rumen Fermentation and Increases Risk of Subacute Ruminal Acidosis during Early Lactation. Animals, 10.","DOI":"10.3390\/ani10081307"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.tvjl.2007.12.021","article-title":"The monitoring, prevention and treatment of sub-acute ruminal acidosis (SARA): A review","volume":"176","author":"Enemark","year":"2008","journal-title":"Vet. J."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Sousa, R.D.S., Oliveira, F.L.C.D., Dias, M.R.B., Minami, N.S., Amaral, L.D., Santos, J.A.A.D., Barr\u00eato J\u00fanior, R.A., Minervino, A.H.H., and Ortolani, E.L. (2020). Characterization of Oligofructose-Induced Acute Rumen Lactic Acidosis and the Appearance of Laminitis in Zebu Cattle. Animals, 10.","DOI":"10.3390\/ani10030429"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Ogunade, I., Pech-Cervantes, A., and Schweickart, H. (2019). Metatranscriptomic Analysis of Sub-Acute Ruminal Acidosis in Beef Cattle. Animals, 9.","DOI":"10.3390\/ani9050232"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"446","DOI":"10.11606\/S1413-95962011000600002","article-title":"Clinical evaluation of hypertonic saline solution treatment for acute ruminal acidosis on cattle","volume":"48","author":"Rodrigues","year":"2011","journal-title":"Braz. J. Vet. Res. an Anim. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.3168\/jds.S0022-0302(97)76026-0","article-title":"Bovine Acidosis: Implications on Laminitis","volume":"80","author":"Nocek","year":"1997","journal-title":"J. Dairy Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2662","DOI":"10.3168\/jds.2011-5080","article-title":"Meta-analysis reveals threshold level of rapidly fermentable dietary concentrate that triggers systemic inflammation in cattle","volume":"95","author":"Zebeli","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"384","DOI":"10.11606\/S1413-95962011000500005","article-title":"Estudo retrospectivo de abscessos hep\u00e1ticos em bovinos abatidos em um frigor\u00edfico paulista","volume":"48","author":"Maschio","year":"2011","journal-title":"Braz. J. Vet. Res. Anim. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"253","DOI":"10.11606\/issn.1678-4456.bjvras.2010.26823","article-title":"Clinical evaluation of zebuine and taurine cattle with acute rumen lactic acidosis","volume":"47","author":"Ortolani","year":"2010","journal-title":"Braz. J. Vet. Res. Anim."},{"key":"ref_17","unstructured":"Rosenberger, G., and Dirksen, G. (1993). Exame Clinico dos Bovinos, Koogan."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s13028-015-0128-9","article-title":"Indicators of induced subacute ruminal acidosis (SARA) in Danish Holstein cows","volume":"57","author":"Danscher","year":"2015","journal-title":"Acta Vet. Scand."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1590\/1678-6366","article-title":"Behaviour of confined sheep fed with different concentrate sources","volume":"66","author":"Minervino","year":"2014","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.3168\/jds.S0022-0302(61)89956-6","article-title":"Volatile Fatty Acid Analyses of Blood and Rumen Fluid by Gas Chromatography","volume":"44","author":"Erwin","year":"1961","journal-title":"J. Dairy Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e8997","DOI":"10.7717\/peerj.8997","article-title":"Biochemical parameters, dynamic tensiometry and circulating nucleic acids for cattle blood analysis: A review","volume":"8","author":"Zaitsev","year":"2020","journal-title":"PeerJ"},{"key":"ref_22","first-page":"462","article-title":"Induction of lactic acidosis in cattle with sucrose: Relationship between dose, rumen fluid pH and animal size","volume":"37","author":"Ortolani","year":"1995","journal-title":"Vet. Hum. Toxicol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"856","DOI":"10.3168\/jds.S0022-0302(07)71569-2","article-title":"Ruminal lipopolysaccharide concentration and inflammatory response during grain-induced subacute ruminai acidosis in dairy cows","volume":"90","author":"Gozho","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.3168\/jds.2008-1656","article-title":"Alfalfa pellet-induced subacute ruminal acidosis in dairy cows increases bacterial endotoxin in the rumen without causing inflammation","volume":"92","author":"Khafipour","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"353","DOI":"10.4141\/cjas2012-004","article-title":"Evaluation of diagnostic measures for subacute ruminal acidosis in dairy cows","volume":"92","author":"Li","year":"2012","journal-title":"Can. J. Anim. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"E17","DOI":"10.3168\/jds.2006-478","article-title":"Ruminal Acidosis in Beef Cattle: The Current Microbiological and Nutritional Outlook","volume":"90","author":"Nagaraja","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1126\/science.1058830","article-title":"Factors that alter rumen microbial ecology","volume":"292","author":"Russell","year":"2001","journal-title":"Science"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Reis, L.F., Sousa, R.S., Oliveira, F.L.C., Rodrigues, F.A.M.L., Ara\u00fajo, C.A.S.C., Meira-J\u00fanior, E.B.S., Barr\u00eato-J\u00fanior, R.A., Mori, C.S., Minervino, A.H.H., and Ortolani, E.L. (2018). Comparative assessment of probiotics and monensin in the prophylaxis of acute ruminal lactic acidosis in sheep. BMC Vet. Res., 14.","DOI":"10.1186\/s12917-017-1264-4"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.cvfa.2017.06.004","article-title":"Diagnosis and Management of Subacute Ruminal Acidosis in Dairy Herds","volume":"33","author":"Oetzel","year":"2017","journal-title":"Vet. Clin. N. Am.-Food Anim. Pract."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/0377-8401(89)90150-8","article-title":"The effects of starch- and pectin-rich diets on quantitative aspects of digestion in sheep","volume":"24","author":"Yosef","year":"1989","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_31","unstructured":"Hungate, R.E. (1966). The Rumen and its Microbes, Elsevier."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.2527\/2000.78123155x","article-title":"Evaluation of models of acute and subacute acidosis on dry matter intake, ruminal fermentation, blood chemistry, and endocrine profiles of beef steers","volume":"78","author":"Brown","year":"2000","journal-title":"J. Anim. Sci."},{"key":"ref_33","unstructured":"Radostits, O.M., Gay, C.C., Hinchcliff, K.W., and Constable, P.D. (2007). Veterinary Medicine: A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats, and Horses, Saunders Elsevier. [10th ed.]."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.3168\/jds.2008-1414","article-title":"Meta-analysis of the influence of Saccharomyces cerevisiae supplementation on ruminal parameters and milk production of ruminants","volume":"92","author":"Desnoyers","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1136\/vr.130.16.343","article-title":"Ruminal lactic acidosis in sheep and goats","volume":"130","author":"Braun","year":"1992","journal-title":"Vet. Rec."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"437","DOI":"10.4141\/cjas85-051","article-title":"Influence of level of feed intake on digestion and bacterial yield in the forestomachs of dairy cattle","volume":"65","author":"Robinson","year":"1985","journal-title":"Can. J. Anim. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1079\/BJN19900029","article-title":"Factors affecting the voluntary intake of food by sheep","volume":"64","author":"Carter","year":"1990","journal-title":"Br. J. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3958","DOI":"10.3168\/jds.2008-1347","article-title":"Repeated ruminal acidosis challenges in lactating dairy cows at high and low risk for developing acidosis: Feed sorting","volume":"91","author":"DeVries","year":"2008","journal-title":"J. Dairy Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"195782","DOI":"10.1155\/2014\/195782","article-title":"Comparison of rumen fluid pH by continuous telemetry system and bench pH meter in sheep with different ranges of ruminal pH","volume":"2014","author":"Reis","year":"2014","journal-title":"Sci. World J."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1590\/S0102-09352007000400024","article-title":"Uso de bicarbonato e lactato-L para corre\u00e7\u00e3o da acidose metab\u00f3lica sist\u00eamica em bovinos com acidose l\u00e1ctica ruminal aguda","volume":"59","author":"Leal","year":"2007","journal-title":"Arq. Bras. Med. Vet. Zootec."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/1\/21\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:45:50Z","timestamp":1760179550000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/1\/21"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,24]]},"references-count":40,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,1]]}},"alternative-id":["ani11010021"],"URL":"https:\/\/doi.org\/10.3390\/ani11010021","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,24]]}}}