{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:29:29Z","timestamp":1788326969579,"version":"build-2803163510"},"reference-count":57,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T00:00:00Z","timestamp":1608768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conseller\u00eda de Econom\u00eda e Industria (Xunta de Galicia), Spain","award":["10MMA020E"],"award-info":[{"award-number":["10MMA020E"]}]},{"name":"Programa de Consolidaci\u00f3n e Estructuraci\u00f3n de Unidades de Investigaci\u00f3n Competitivas, Spain","award":["GPC2015\/034"],"award-info":[{"award-number":["GPC2015\/034"]}]},{"name":"Stolt Sea Farm","award":["10MMA020E"],"award-info":[{"award-number":["10MMA020E"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The high incidence of skeletal anomalies in Senegalese sole (Solea senegalensis) still constitutes a bottleneck constraining its production. There are diverse commercially available products for the enrichment of live preys, but few reports of their influence on skeletogenesis in Senegalese sole. This study evaluated the presence of vertebral anomalies in postlarvae and juvenile Senegalese sole fed with Artemia spp. metanauplii enriched with four commercial products (EA, EB, EC, and ED) in a fish farm. The most frequent alterations consisted of deformations of the neural\/haemal arches and spines and fusions and deformations of hypurals, epural, or parhypural. The correspondence analysis ordered fish from each age in separated semiaxis, indicating the presence of different anomaly patterns for the two sampled stages. The results showed only very light changes in the frequency of vertebral abnormalities among tested enrichment products, i.e., individuals from EC and EA lots displayed less vertebral body anomalies and\/or vertebral column deviations at 31 and 105 days after hatching, respectively. The existence of a large shared malformation pattern in all the experimental groups leads to impute to the rearing conditions as the main driving factor of the onset of such group of anomalies, probably masking some dietary effect.<\/jats:p>","DOI":"10.3390\/ani11010022","type":"journal-article","created":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T22:56:45Z","timestamp":1608850605000},"page":"22","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Skeletal Anomalies in Senegalese Sole (Solea senegalensis, Kaup) Fed with Different Commercial Enriched Artemia: A Study in Postlarvae and Juveniles"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2451-5039","authenticated-orcid":false,"given":"Ana Manuela","family":"de Azevedo","sequence":"first","affiliation":[{"name":"Department of Anatomy, Animal Production and Clinical Veterinary Sciences, Faculty of Veterinary Science, Campus Universitario S\/N, University of Santiago de Compostela, 27002 Lugo, Spain"},{"name":"Biology Department, Ghent University, Ledeganckstraat 35, 9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7459-0352","authenticated-orcid":false,"given":"Ana Paula","family":"Losada","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Animal Production and Clinical Veterinary Sciences, Faculty of Veterinary Science, Campus Universitario S\/N, University of Santiago de Compostela, 27002 Lugo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Ferreiro","sequence":"additional","affiliation":[{"name":"Stolt Sea Farm, Edificio Quercus, C\/Letonia n.\u00b0 2, 15701 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Riaza","sequence":"additional","affiliation":[{"name":"Stolt Sea Farm, Edificio Quercus, C\/Letonia n.\u00b0 2, 15701 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanesa","family":"Losada","sequence":"additional","affiliation":[{"name":"ANFACO-CECOPESCA, Estrada Colexio Universitario, 16, 36310 Vigo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tommaso","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Boglione","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Animal Production and Clinical Veterinary Sciences, Faculty of Veterinary Science, Campus Universitario S\/N, University of Santiago de Compostela, 27002 Lugo, Spain"},{"name":"Aquaculture Institute, Campus Vida, R\u00faa de Constantino Candeira, 15705 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Isabel","family":"Quiroga","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Animal Production and Clinical Veterinary Sciences, Faculty of Veterinary Science, Campus Universitario S\/N, University of Santiago de Compostela, 27002 Lugo, Spain"},{"name":"Aquaculture Institute, Campus Vida, R\u00faa de Constantino Candeira, 15705 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,24]]},"reference":[{"key":"ref_1","unstructured":"FAO (2020). Fishery and Aquaculture Statistics. Global Aquaculture Production 1950\u20132018 (FishstatJ), FAO Fisheries and Aquaculture Department."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S0044-8486(02)00167-9","article-title":"Osteological development and abnormalities of the vertebral column and caudal skeleton in larval and juvenile stages of hatchery-reared Senegal sole (Solea senegalensis)","volume":"211","author":"Gavaia","year":"2002","journal-title":"Aquaculture"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.aquaculture.2009.06.046","article-title":"Effect of dietary vitamin A on Senegalese sole (Solea senegalensis) skeletogenesis and larval quality","volume":"295","author":"Pimentel","year":"2009","journal-title":"Aquaculture"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.aquaculture.2011.11.018","article-title":"Commercial products for Artemia enrichment affect growth performance, digestive system maturation, ossification and incidence of skeletal deformities in Senegalese sole (Solea senegalensis) larvae","volume":"324","author":"Boglino","year":"2012","journal-title":"Aquaculture"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1111\/j.1365-2109.2009.02258.x","article-title":"Comparing skeletal development of wild and hatchery-reared Senegalese sole (Solea senegalensis, Kaup 1858): Evaluation in larval and postlarval stages","volume":"40","author":"Gavaia","year":"2009","journal-title":"Aquac. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1111\/jfd.12575","article-title":"New insight on vertebral anomalies in cultured Senegalese sole (Solea senegalensis, Kaup) at early stages of development","volume":"40","author":"Losada","year":"2017","journal-title":"J. Fish Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1111\/jai.12524","article-title":"Skeletal malformations in Senegalese sole (Solea senegalensis Kaup, 1858): Gross morphology and radiographic correlation","volume":"30","author":"Losada","year":"2014","journal-title":"J. Appl. Ichthyol."},{"key":"ref_8","first-page":"S99","article-title":"Skeletal anomalies in reared European fish larvae and juveniles. Part 1: Normal and anomalous skeletogenic processes","volume":"5","author":"Boglione","year":"2013","journal-title":"Rev. Aquac."},{"key":"ref_9","first-page":"S121","article-title":"Skeletal anomalies in reared European fish larvae and juveniles. Part 2: Main typologies, occurrences and causative factors","volume":"5","author":"Boglione","year":"2013","journal-title":"Rev. Aquac."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0044-8486(03)00507-6","article-title":"Nutritional components affecting skeletal development in fish larvae","volume":"227","author":"Cahu","year":"2003","journal-title":"Aquaculture"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.aquaculture.2007.04.030","article-title":"Nutritional physiology during development of Senegalese sole (Solea senegalensis)","volume":"268","author":"Ribeiro","year":"2007","journal-title":"Aquaculture"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.aquaculture.2007.02.053","article-title":"Role of nutrients in skeletal metabolism and pathology in fish\u2014An overview","volume":"267","author":"Lall","year":"2007","journal-title":"Aquaculture"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.aquaculture.2009.09.022","article-title":"Effects of dietary phosphorus and calcium level on growth and skeletal development in rainbow trout (Oncorhynchus mykiss) fry","volume":"297","author":"Silva","year":"2009","journal-title":"Aquaculture"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.aquaculture.2010.12.030","article-title":"Overview of vitamin D and C requirements in fish and their influence on the skeletal system","volume":"315","author":"Darias","year":"2011","journal-title":"Aquaculture"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1007\/s10126-014-9571-2","article-title":"Dietary supplementation with vitamin K affects transcriptome and proteome of Senegalese sole, improving larval performance and quality","volume":"16","author":"Richard","year":"2014","journal-title":"Mar. Biotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0044-8486(99)00004-6","article-title":"Lipid conversions during enrichment of Artemia","volume":"174","author":"Navarro","year":"1999","journal-title":"Aquaculture"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"S26","DOI":"10.1111\/j.1753-5131.2012.01086.x","article-title":"Fish larval nutrition and feed formulation: Knowledge gaps and bottlenecks for advances in larval rearing","volume":"5","author":"Hamre","year":"2013","journal-title":"Rev. Aquac."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10695-009-9373-z","article-title":"Novel methodologies in marine fish larval nutrition","volume":"36","author":"Richard","year":"2010","journal-title":"Fish Physiol. Biochem."},{"key":"ref_19","unstructured":"Rodr\u00edguez, J.L., and Peleteiro, J.B. (2014). Cultivo del Lenguado Senegal\u00e9s (Solea senegalensis), Fundaci\u00f3n Observatorio Espa\u00f1ol de Acuicultura. [1st ed.]."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/S0308-8146(00)00155-2","article-title":"Simultaneous HPLC analysis of fat-soluble vitamins in selected animal products after small-scale extraction","volume":"71","author":"Ollilainen","year":"2000","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1002\/(SICI)1097-0010(199803)76:3<477::AID-JSFA975>3.0.CO;2-U","article-title":"Monitoring of the vitamin C content of frozen green beans and Padr\u00f3n peppers by HPLC","volume":"76","year":"1998","journal-title":"J. Sci. Food Agric."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0076-6879(88)67027-3","article-title":"Membrane lipids","volume":"167","author":"Sato","year":"1988","journal-title":"Methods Enzymol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"79","DOI":"10.3109\/10520290009064151","article-title":"Detection of mineralized structures in early stages of development of marine Teleostei using a modified alcian blue-alizarin red double staining technique for bone and cartilage","volume":"75","author":"Gavaia","year":"2000","journal-title":"Biotech. Histochem. Off. Publ. Biol. Stain Comm."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1111\/j.1439-0426.2010.01421.x","article-title":"Double staining protocol for developing European sea bass (Dicentrarchus labrax) larvae","volume":"26","author":"Darias","year":"2010","journal-title":"J. Appl. Ichthyol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Schneider, C.A., Rasband, W.S., and Eliceiri, K.W. (2012). NIH Image to ImageJ: 25 years of image analysis. Nat. Methods, 9.","DOI":"10.1038\/nmeth.2089"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Prestinicola, L., Boglione, C., Makridis, P., Span\u00f2, A., Rimatori, V., Palamara, E., Scardi, M., and Cataudella, S. (2013). Environmental conditioning of skeletal anomalies typology and frequency in gilthead seabream (Sparus aurata L., 1758) juveniles. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0055736"},{"key":"ref_28","unstructured":"RStudio Team (2019). RStudio: Integrated Development Environment for R, RStudio, Inc."},{"key":"ref_29","unstructured":"R Core Team (2019). R: A Language and Environment for Statistical Computing, R Core Team."},{"key":"ref_30","unstructured":"Hammer, \u00d8., Harper, D.A.T., and Ryan, P.D. (2001). PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontol. Electron., 4."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1007\/s10499-009-9296-9","article-title":"Lack of essential fatty acids in live feed during larval and post-larval rearing: Effect on the performance of juvenile Solea senegalensis","volume":"18","author":"Ribeiro","year":"2010","journal-title":"Aquac. Int."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.fsi.2009.11.006","article-title":"Effects of stocking density and feed ration on growth and gene expression in the Senegalese sole (Solea senegalensis): Potential effects on the immune response","volume":"28","author":"Anguis","year":"2010","journal-title":"Fish Shellfish Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1111\/j.1365-2109.2011.03026.x","article-title":"Stocking density affects Senegalese sole (Solea senegalensis, Kaup) growth independently of size dispersion, evaluated using an individual photo-identification technique","volume":"44","author":"Ambrosio","year":"2013","journal-title":"Aquac. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1111\/j.1439-0426.2012.01977.x","article-title":"The effect of dietary arachidonic acid during the Artemia feeding period on larval growth and skeletogenesis in Senegalese sole, Solea senegalensis","volume":"28","author":"Boglino","year":"2012","journal-title":"J. Appl. Ichthyol."},{"key":"ref_35","first-page":"10","article-title":"Effects of pre-weaning feeding frequency on growth, survival, and deformation of Senegalese sole, Solea senegalensis (Kaup, 1858)","volume":"57","author":"Engrola","year":"2005","journal-title":"Isr. J. Aquac. Bamidgeh"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1111\/j.1439-0426.2012.01980.x","article-title":"Vertebral deformities in farmed Atlantic salmon (Salmo salar L.)\u2014Etiology and pathology","volume":"28","author":"Fjelldal","year":"2012","journal-title":"J. Appl. Ichthyol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Martini, A., Huysseune, A., Witten, P.E., and Boglione, C. (2020). Plasticity of the skeleton and skeletal deformities in zebrafish (Danio rerio) linked to rearing density. J. Fish Biol., 1\u201316.","DOI":"10.1111\/jfb.14272"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1111\/j.1095-8649.2000.tb02127.x","article-title":"Skeletal characterization of wild and reared zebrafish: Anomalies and meristic characters","volume":"56","author":"Ferreri","year":"2000","journal-title":"J. Fish Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.aquaculture.2010.05.034","article-title":"Effect of daily thermo- and photo-cycles of different light spectrum on the development of Senegal sole (Solea senegalensis) larvae","volume":"306","author":"Villamizar","year":"2010","journal-title":"Aquaculture"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1111\/j.1439-0426.2012.01996.x","article-title":"Effect of egg incubation temperature on the occurrence of skeletal deformities in Solea senegalensis","volume":"28","author":"Campos","year":"2012","journal-title":"J. Appl. Ichthyol."},{"key":"ref_41","first-page":"2062","article-title":"Defective skeletogenesis and oversized otoliths in fish early stages in a changing ocean","volume":"217","author":"Pimentel","year":"2014","journal-title":"J. Exp. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1016\/j.chemosphere.2019.06.183","article-title":"Effects of triclosan on early development of Solea senegalensis: From biochemical to individual level","volume":"235","author":"Quintaneiro","year":"2019","journal-title":"Chemosphere"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/j.1469-185X.2009.00077.x","article-title":"A comparative view on mechanisms and functions of skeletal remodelling in teleost fish, with special emphasis on osteoclasts and their function","volume":"84","author":"Witten","year":"2009","journal-title":"Biol. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.1439-0426.2010.01423.x","article-title":"Phenotypic plasticity and mechano-transduction in the teleost skeleton","volume":"26","author":"Fiaz","year":"2010","journal-title":"J. Appl. Ichthyol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1111\/j.1439-0426.2012.01991.x","article-title":"The structure-mechanics relationship and the response to load of the acellular bone of neoteleost fish: A review","volume":"28","author":"Dean","year":"2012","journal-title":"J. Appl. Ichthyol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.aquaculture.2006.05.005","article-title":"Vertebrae fusion in Atlantic salmon (Salmo salar): Development, aggravation and pathways of containment","volume":"258","author":"Witten","year":"2006","journal-title":"Aquaculture"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1643\/CG-14-140","article-title":"Teleost skeletal plasticity: Modulation, adaptation, and remodelling","volume":"103","author":"Witten","year":"2015","journal-title":"Copeia"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.ydbio.2014.08.007","article-title":"In-vivo imaging of the fracture healing in medaka revealed two types of osteoclasts before and after the callus formation by osteoblasts","volume":"394","author":"Takeyama","year":"2014","journal-title":"Dev. Biol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Fragkoulis, S., Printzi, A., Geladakis, G., Katribouzas, N., and Koumoundouros, G. (2019). Recovery of haemal lordosis in Gilthead seabream (Sparus aurata L.). Sci. Rep., 9.","DOI":"10.1038\/s41598-019-46334-1"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Fraser, T.W.K., Witten, P.E., Albrektsen, S., Breck, O., Fontanillas, R., Nankervis, L., Thomsen, T.H., Koppe, W., Sambraus, F., and Fjelldal, P.G. (2019). Phosphorus nutrition in farmed Atlantic salmon (Salmo salar): Life stage and temperature effects on bone pathologies. Aquaculture, 511.","DOI":"10.1016\/j.aquaculture.2019.734246"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"117","DOI":"10.3354\/dao03110","article-title":"Skeletal anomalies in reared Senegalese sole Solea senegalensis juveniles: A radiographic approach","volume":"124","author":"Losada","year":"2017","journal-title":"Dis. Aquat. Org."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.aquaculture.2011.12.043","article-title":"Inadequate phosphorus nutrition in juvenile Atlantic salmon has a negative effect on long-term bone health","volume":"334\u2013337","author":"Fjelldal","year":"2012","journal-title":"Aquaculture"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1017\/S0007114512003935","article-title":"Effects of dietary DHA and \u03b1-tocopherol on bone development, early mineralisation and oxidative stress in Sparus aurata (Linnaeus, 1758) larvae","volume":"109","author":"Izquierdo","year":"2013","journal-title":"Br. J. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.aquaculture.2008.10.010","article-title":"Effect of DHA content in rotifers on the occurrence of skeletal deformities in red porgy Pagrus pagrus (Linnaeus, 1758)","volume":"287","author":"Roo","year":"2009","journal-title":"Aquaculture"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1111\/jfb.12870","article-title":"A primary phosphorus-deficient skeletal phenotype in juvenile Atlantic salmon Salmo salar: The uncoupling of bone formation and mineralization","volume":"88","author":"Witten","year":"2015","journal-title":"J. Fish Biol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Witten, P.E., Fjelldal, P.G., Huysseune, A., McGurk, C., Obach, A., and Owen, M.A. (2019). Bone without minerals and its secondary mineralization in Atlantic salmon (Salmo salar): The recovery from phosphorus deficiency. J. Exp. Biol., 222.","DOI":"10.1242\/jeb.188763"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1111\/j.1439-0426.2010.01432.x","article-title":"Senegalese sole bone tissue originated from chondral ossification is more sensitive than dermal bone to high vitamin A content in enriched Artemia","volume":"26","author":"Gisbert","year":"2010","journal-title":"J. Appl. Ichthyol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/1\/22\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:45:54Z","timestamp":1760179554000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/1\/22"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,24]]},"references-count":57,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,1]]}},"alternative-id":["ani11010022"],"URL":"https:\/\/doi.org\/10.3390\/ani11010022","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,24]]}}}