{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T02:39:10Z","timestamp":1786588750376,"version":"build-2736575974"},"reference-count":34,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T00:00:00Z","timestamp":1610582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Sience Foundation","award":["19-16-00009"],"award-info":[{"award-number":["19-16-00009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The aim of this study was to create balanced media for the cryopreservation of rooster semen in pellets to maintain the functional state of the sperm after thawing. Fructose was replaced by trehalose in experimental media in proportions of 10% (LCM-T10) and 20% (LCM-T20), while LCM was used as a control. After artificial insemination of the hens, the eggs were incubated (n = 400). To determine the functional safety of spermatozoa in the genital tract of hens after 5, 10, and 15 days from the last insemination, we used a method for assessing the interaction of sperm with the perivitelline membrane. Significantly higher rates of egg fertilization (82\u201386%) were obtained when using LCM-T10 and LCM-T20 compared to control (79%, p &lt; 0.05). Egg fertility on the 5th day from the last insemination with the LCM-T20 diluent reached 100% versus 86% in the control; on the 10th day, the fertility rates were 55% versus 20%, respectively. The best results for fertility duration were obtained by freezing spermatozoa with LCM-T20 medium. The numbers of interaction points of spermatozoa with the perivitelline membrane were as follows: on the 5th day from the last insemination with LCM-T20\u2014461.5 \u00b1 11.5 holes\/cm2 (LCM-control\u201413.7 \u00b1 2.7 holes\/cm2), p &lt; 0.01; on the 10th day with LCM-T20\u2014319.3 \u00b1 12.9 holes\/cm2 (LCM-control\u201414.9 \u00b1 3.5 holes\/cm2); and on the 15th day with LCM-T20\u2014345.2 \u00b1 11.1 holes\/cm2 (LCM-control\u20140 holes\/cm2). In conclusion, the use of trehalose in LCM diluent medium can increase the fertility of frozen\/thawed sperm and the duration of their fertility in the genital tract of hens.<\/jats:p>","DOI":"10.3390\/ani11010189","type":"journal-article","created":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T11:39:14Z","timestamp":1610624354000},"page":"189","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Effects of Saccharides Supplementation in the Extender of Cryopreserved Rooster (Gallus domesticus) Semen on the Fertility of Frozen\/Thawed Spermatozoa"],"prefix":"10.3390","volume":"11","author":[{"given":"Olga","family":"Stanishevskaya","sequence":"first","affiliation":[{"name":"Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Russian Research Institute of Farm Animal Genetics and Breeding, 196625 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1905-6373","authenticated-orcid":false,"given":"Yulia","family":"Silyukova","sequence":"additional","affiliation":[{"name":"Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Russian Research Institute of Farm Animal Genetics and Breeding, 196625 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolai","family":"Pleshanov","sequence":"additional","affiliation":[{"name":"Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Russian Research Institute of Farm Animal Genetics and Breeding, 196625 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Kurochkin","sequence":"additional","affiliation":[{"name":"Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Russian Research Institute of Farm Animal Genetics and Breeding, 196625 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Fedorova","sequence":"additional","affiliation":[{"name":"Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Russian Research Institute of Farm Animal Genetics and Breeding, 196625 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoya","family":"Fedorova","sequence":"additional","affiliation":[{"name":"Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Russian Research Institute of Farm Animal Genetics and Breeding, 196625 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oksana","family":"Perinek","sequence":"additional","affiliation":[{"name":"Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Russian Research Institute of Farm Animal Genetics and Breeding, 196625 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Prituzhalova","sequence":"additional","affiliation":[{"name":"Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Russian Research Institute of Farm Animal Genetics and Breeding, 196625 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inessa","family":"Meftakh","sequence":"additional","affiliation":[{"name":"Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, Russian Research Institute of Farm Animal Genetics and Breeding, 196625 Saint-Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,14]]},"reference":[{"key":"ref_1","unstructured":"Scherf, B.D., Pilling, D., and FAO Commission on Genetic Resources for Food and Agriculture Assessments (2024, July 07). The Second Report on the State of the World\u2019s Animal Genetic Resources for Food and Agriculture. Rome. Available online: http:\/\/www.fao.org\/3\/a-i4787e\/index.html."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.4081\/ijas.2007.1s.63","article-title":"Heterozygosity analysis of Bionda Piemontese and Bianca di Saluzzo chicken breeds by microsatellites markers: A preliminary study","volume":"6","author":"Cavalchini","year":"2007","journal-title":"Ital. J. Anim. 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