{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:54:41Z","timestamp":1777596881556,"version":"3.51.4"},"reference-count":97,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T00:00:00Z","timestamp":1612828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Granada-CEI BioTic","award":["P-BS-37"],"award-info":[{"award-number":["P-BS-37"]}]},{"name":"Junta de Andalucia Proyectos de Excelencia 2011","award":["RNM-8147"],"award-info":[{"award-number":["RNM-8147"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Phytobiotics (bioactive compounds extracted from plants) are one of the explored alternatives to antibiotics in poultry and livestock due to their antimicrobial activity and its positive effects on gut microbiota and productive properties. In this study, we supplemented a product based on garlic and onion compounds in the diet to laying hens at the beginning of their productive life (from 16 to 20 weeks post-hatching). The experimental group showed a significant increase in the number of eggs laid and in their size, produced in one month compared to the control. This increase in production was accompanied by microbiota changes in the ileum and cecum by means of high throughput sequencing analyses. These bacterial shifts in the ileum were mainly the result of compositional changes in the rare biosphere (unweighted UniFrac), while in the cecum, treatment affected both majority and minority bacterial groups (weighted and unweighted UniFrac). These changes in the microbiota suggest an improvement in food digestibility. The relative abundance of Lactococcus in the ileum and Lactobacillus in the cecum increased significantly in the experimental group. The relative abundance of these bacterial genera are known to have positive effects on the hosts. These results are very promising for the use of these compounds in poultry for short periods.<\/jats:p>","DOI":"10.3390\/ani11020448","type":"journal-article","created":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T23:43:16Z","timestamp":1612914196000},"page":"448","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Allium-Based Phytobiotic Enhances Egg Production in Laying Hens through Microbial Composition Changes in Ileum and Cecum"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1610-6358","authenticated-orcid":false,"given":"Miguel","family":"Rabelo-Ruiz","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Universidad de Granada, Avda. Fuentenueva, s\/n, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Jos\u00e9","family":"Ariza-Romero","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Biotecnolog\u00eda, DMC Research Center, Camino de Jayena s\/n, 18620 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Jes\u00fas","family":"Zurita-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Biotecnolog\u00eda, DMC Research Center, Camino de Jayena s\/n, 18620 Granada, Spain"},{"name":"\u00c1rea de Endocrinolog\u00eda Molecular y Celular, Fundaci\u00f3n Instituto de Investigaci\u00f3n Sanotaria de Santiago de Compostela (FIDIS), Complejo Hospitalario Universitario De Santiago (CHUS), Servicio Gallego de Salud (SERGAS), 15706 Santiago de Compostela, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2822-4885","authenticated-orcid":false,"given":"Antonio Manuel","family":"Mart\u00edn-Platero","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Universidad de Granada, Avda. Fuentenueva, s\/n, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1691-2587","authenticated-orcid":false,"given":"Alberto","family":"Ba\u00f1os","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Biotecnolog\u00eda, DMC Research Center, Camino de Jayena s\/n, 18620 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mercedes","family":"Maqueda","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Universidad de Granada, Avda. Fuentenueva, s\/n, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Valdivia","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Universidad de Granada, Avda. Fuentenueva, s\/n, 18071 Granada, Spain"},{"name":"Instituto de Biotecnolog\u00eda, Universidad de Granada, Avda. Fuentenueva, s\/n, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Mart\u00ednez-Bueno","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Universidad de Granada, Avda. Fuentenueva, s\/n, 18071 Granada, Spain"},{"name":"Instituto de Biotecnolog\u00eda, Universidad de Granada, Avda. Fuentenueva, s\/n, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4648-7988","authenticated-orcid":false,"given":"Juan Manuel","family":"Peralta-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Universidad de Granada, Avda. Fuentenueva, s\/n, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,9]]},"reference":[{"key":"ref_1","first-page":"181","article-title":"The worldwide threat of antimicrobial resistance","volume":"95","author":"Wise","year":"2008","journal-title":"Curr. Sci."},{"key":"ref_2","unstructured":"World Health Organization (2018, May 17). Antimicrobial Resistance. Available online: http:\/\/www.who.int\/antimicrobial-resistance\/en\/."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S122","DOI":"10.1038\/nm1145","article-title":"Antibacterial resistance worldwide: Causes, challenges and responses","volume":"10","author":"Levy","year":"2004","journal-title":"Nat. Med."},{"key":"ref_4","unstructured":"European Commission (2018). 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