{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T04:24:19Z","timestamp":1771302259228,"version":"3.50.1"},"reference-count":49,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T00:00:00Z","timestamp":1615161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>A wide range of mammalian hybrids has recently been found by chance or through population-screening programs, but studies about their fertilizing capacity remain scarce and incomplete. Most of them are assumed to be sterile due to meiotic arrest caused by the failure of chromosome pairings. In this study, we evaluated both sperm meiotic segregation, by 2D fluorescent in situ hybridization (FISH) analysis, and sperm quality (Sperm Chromatin Structure Assay) by flow cytometer in a fertile boar\u2013pig hybrid (2n = 37,XY) originating from a Nero Siciliano pig breed (Sus scrofa domesticus) and a wild boar (Sus scrofa ferus). Spermatozoa were also separated by a dual-layer (75\u201360%) discontinuous Percoll gradient, resulting in two fractions with a significantly better overall quality in the motile sperm fraction. These data were confirmed by FISH analysis also, where the frequencies of spermatozoa with a regular chromosome composition were 27% in total sperm fraction and 64% in motile sperm fraction. We also evaluated the nuclear architecture in all counted spermatozoa, showing a chromatin distribution changing when chromosome abnormalities occur. Our results demonstrate that the chromosome pairing has a minimal effect on the sperm segregation and semen quality of a boar\u2013pig hybrid, making it fertile and harmful for the conservation of autochthonous pig breeds.<\/jats:p>","DOI":"10.3390\/ani11030738","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T23:04:39Z","timestamp":1615244679000},"page":"738","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Sperm Nuclei Analysis and Nuclear Organization of a Fertile Boar\u2013Pig Hybrid by 2D FISH on Both Total and Motile Sperm Fractions"],"prefix":"10.3390","volume":"11","author":[{"given":"Viviana","family":"Genualdo","sequence":"first","affiliation":[{"name":"Institute for Animal Production System in Mediterranean Environment, National Research Council, Portici, 80055 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2876-999X","authenticated-orcid":false,"given":"Federica","family":"Turri","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Biology and Biotechnology, National Research Council, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavia","family":"Pizzi","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Biology and Biotechnology, National Research Council, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2326-6701","authenticated-orcid":false,"given":"Bianca","family":"Castiglioni","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Biology and Biotechnology, National Research Council, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8408-7443","authenticated-orcid":false,"given":"Donata","family":"Marletta","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food and Environment, University of Catania, 95131 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Iannuzzi","sequence":"additional","affiliation":[{"name":"Institute for Animal Production System in Mediterranean Environment, National Research Council, Portici, 80055 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jhered\/esl051","article-title":"A comprehensive evaluation of cattle introgression into US federal bison herds","volume":"98","author":"Halbert","year":"2007","journal-title":"J. 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