{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:06:32Z","timestamp":1792022792071,"version":"4.4.0"},"reference-count":33,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T00:00:00Z","timestamp":1615680000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The main objective of the present study was to evaluate the beta-hydroxybutyrate (BHB) and fat to protein content (F:P) ratio patterns in the milk of Holstein cows with postpartum diseases throughout the first five months of lactation. This prospective study was performed at Vestjyske Dyrlaeger ApS (N\u00f8rre Nebel, Denmark). The milk fat, protein, and BHB were evaluated in the Danish Eurofins laboratory according to the monthly averaged days in milk (DIM1 to 5). According to clinical records, five groups were formed: A (control group; cows without diseases; n = 32), B (cows with left displaced abomasum -LDA- and concomitant diseases; n = 25); C (cows with other diseases up to DIM3; n = 13); D (cows with foot disorders up to DIM3; n = 26); and E (cows with disease manifestations in DIM4 and DIM5; n = 26). All the sick cows were treated after diagnosis, and laparoscopy was performed on cows with LDA. In group B, a higher concentration of BHB (0.18 \u00b1 0.02 mmol\/L; p &lt; 0.001) was observed than in the control group (0.07 \u00b1 0.02 mmol\/L; p &lt; 0.001) in DIM1, presenting an odds ratio (OR) = 8.9. In all groups, BHB decreased to 0.03\u20130.05 mmol\/L (p &lt; 0.05) since DIM3. The F:P ratio was higher in group B (1.77 \u00b1 0.07) than in group A (1.32 \u00b1 0.06; p &lt; 0.05) in DIM1. A similar profile is observed in DIM2. It was observed that animals in group B were four to six times more likely to have a F:P ratio \u22651.29 during DIM1 (OR = 4.0; 95% CI:1.3\u201314.4; p = 0.01) and DIM2 (OR = 5.9; 95% CI %:1.9\u201321.9; p &lt; 0.01), than cows in group A. There were also moderate and high correlations between the F:P ratio and the BHB for DIM1 (r = 0.57; r2 = 0.33; RSD = 0.09; p &lt; 0.001) and DIM2 (r = 0.78; r2 = 0.60; RSD = 0.07; p &lt; 0.001), respectively. We concluded that animals affected by LDA in the postpartum period have a higher concentration of BHB in milk in DIM1 and all treated animals quickly recover BHB levels up to DIM3. The F:P ratio is a viable and economic indicator, mainly in DIM1 and DIM2, to estimate BHB concentration and energy balance in cows with LDA and other postpartum diseases.<\/jats:p>","DOI":"10.3390\/ani11030816","type":"journal-article","created":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T22:13:10Z","timestamp":1615759990000},"page":"816","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Milk Beta-Hydroxybutyrate and Fat to Protein Ratio Patterns during the First Five Months of Lactation in Holstein Dairy Cows Presenting Treated Left Displaced Abomasum and Other Post-Partum Diseases"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0622-7348","authenticated-orcid":false,"given":"Mariana Alves Caipira","family":"Lei","sequence":"first","affiliation":[{"name":"Department of Zootechnics, School of Agricultural and Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4997-3933","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, School of Agricultural and Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1676","DOI":"10.1017\/S175173111900034X","article-title":"Invited review: \u03b2-hydroxybutyrate concentration in blood and milk and its associations with cow performance","volume":"13","author":"Benedet","year":"2019","journal-title":"Animal"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2925","DOI":"10.3168\/jds.2012-6035","article-title":"Prevalence of subclinical ketosis and relationships with postpartum diseases in European dairy cows","volume":"96","author":"Suthar","year":"2013","journal-title":"J. Dairy Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1093\/tas\/txy102","article-title":"Prevalence of subclinical ketosis and production diseases in dairy cows in Central and South America, Africa, Asia, Australia, New Zealand, and Eastern Europe","volume":"3","author":"Brunner","year":"2019","journal-title":"Transl. Anim. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"330","DOI":"10.3168\/jds.2013-7222","article-title":"Induced hyperketonemia affects the mammary immune response during lipopolysaccharide challenge in dairy cows","volume":"97","author":"Zarrin","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.tvjl.2015.02.013","article-title":"Genetics of bovine abomasal displacement","volume":"204","author":"Zerbin","year":"2015","journal-title":"Vet. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1292\/jvms.19-0378","article-title":"Identification of disordered metabolic networks in postpartum dairy cows with left displacement of the abomasum through integrated metabolomics and pathway analyses","volume":"82","author":"Guo","year":"2020","journal-title":"J. Vet. Med. Sci."},{"key":"ref_7","first-page":"9","article-title":"Abomasopessilaparoscopica one-step","volume":"12","author":"Barisani","year":"2017","journal-title":"Summa Animali Reddito"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.cvfa.2008.02.014","article-title":"Minimally invasive field abomasopexy techniques for correction and fixation of left displacement of the abomasum in dairy cows","volume":"24","author":"Newman","year":"2008","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_9","unstructured":"Smith, R.A. Comparison of two techniques for laparoscopic abomasopexy for correction of left displaced abomasum in dairy cows. Proceedings of the Thirty-Sixth Annual Conference, Columbus, OH, USA, 18\u201320 September 2003."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"198","DOI":"10.12968\/live.2017.22.4.198","article-title":"LDAs in cattle\u2014What method should we use? A report from a BCVA congress workshop","volume":"22","author":"Wapenaar","year":"2017","journal-title":"Livestock"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4816","DOI":"10.3168\/jds.2015-10477","article-title":"Development of Fourier transform mid-infrared calibrations to predict acetone, \u03b2-hydroxybutyrate, and citrate contents in bovine milk through a European dairy network","volume":"99","author":"Grelet","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.3168\/jds.2018-14778","article-title":"Short communication: Validation of a test-day milk test for \u03b2-hydroxybutyrate for identifying cows with hyperketonemia","volume":"102","author":"Renaud","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"295","DOI":"10.3168\/jds.S0022-0302(99)75236-7","article-title":"Postpartum body condition score and results from the first test day milk as predictors of disease, fertility, yield, and culling in commercial dairy herds","volume":"82","author":"Heuer","year":"1999","journal-title":"J. Dairy Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1159\/000438569","article-title":"B-Hydroxybutyrate facilitates fatty acids synthesis mediated by sterol regulatory element-binding protein1 in bovine mammary epithelial cells","volume":"37","author":"Zhang","year":"2015","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1820","DOI":"10.3168\/jds.2010-3707","article-title":"Performance and metabolic profile of dairy cows during a lactational and deliberately induced negative energy balance with subsequent realimentation","volume":"94","author":"Gross","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8417","DOI":"10.3168\/jds.2018-16070","article-title":"Short communication: Assessing the accuracy of inline milk fat-to-protein ratio data as an indicator of hyperketonemia in dairy cows in herds with automated milking systems","volume":"102","author":"King","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3065","DOI":"10.3168\/jds.2009-2847","article-title":"Short communication: Ketone body concentration in milk determined by Fourier transform infrared spectroscopy: Value for the detection of hyperketonemia in dairy cows","volume":"93","author":"Horneman","year":"2010","journal-title":"J. Dairy Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3364","DOI":"10.3168\/jds.2013-6744","article-title":"Accuracy of milk ketone bodies from flow-injection analysis for the diagnosis of hyperketonemia in dairy cows","volume":"97","author":"Dubuc","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6298","DOI":"10.3168\/jds.2016-12458","article-title":"Genetic parameters of blood \u03b2-hydroxybutyrate predicted from milk infrared spectra and clinical ketosis, and their associations with milk production traits in Norwegian Red cows","volume":"100","author":"Belay","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"7286","DOI":"10.3168\/jds.2014-8405","article-title":"Genetic analysis of milk \u03b2-hydroxybutyrate and its association with fat-to-protein ratio, body condition score, clinical ketosis, and displaced abomasum in early first lactation of Canadian Holsteins","volume":"97","author":"Koeck","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.tvjl.2019.04.001","article-title":"Prevalence of excessive negative energy balance in commercial United Kingdom dairy herds","volume":"248","author":"Macraea","year":"2019","journal-title":"Vet. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7547","DOI":"10.3168\/jds.2014-8237","article-title":"Diseases, reproductive performance, and changes in milk production associated with subclinical ketosis in dairy cows: A meta-analysis and review","volume":"97","author":"Raboisson","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5056","DOI":"10.3168\/jds.2012-5443","article-title":"Epidemiology of subclinical ketosis in early lactation dairy cattle","volume":"95","author":"McArt","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5462","DOI":"10.3168\/jds.2016-12237","article-title":"Short communication: Associations between blood glucose concentration, onset of hyperketonemia, and milk production in early lactation dairy cows","volume":"100","author":"Ruoff","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5604","DOI":"10.3168\/jds.2015-10509","article-title":"Association of rumination time with subclinical ketosis in transition dairy cows","volume":"99","author":"Kaufman","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"571","DOI":"10.3168\/jds.2008-1507","article-title":"Impact of hyperketonemia in early lactation dairy cows on health and production","volume":"92","author":"Duffield","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.prevetmed.2011.09.005","article-title":"Risk factors for displaced abomasum or ketosis in Swedish dairy herds","volume":"103","author":"Hultgren","year":"2012","journal-title":"Prev. Vet. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0749-0720(15)30103-1","article-title":"Subclinical ketosis in lactating dairy cattle","volume":"16","author":"Duffield","year":"2000","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"s75","DOI":"10.1017\/S175173111800349X","article-title":"Review: Metabolic challenges in lactating dairy cows and their assessment via established and novel indicators in milk","volume":"13","author":"Gross","year":"2019","journal-title":"Animal"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Zhang, G., and Ametaj, B.N. (2020). Ketosis an old story under a new approach. Dairy, 1.","DOI":"10.3390\/dairy1010005"},{"key":"ref_31","first-page":"1","article-title":"Fatty acids in bovine milk fat fatty acids in bovine milk fat","volume":"52","year":"2008","journal-title":"J. Food Nutr. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.3168\/jds.S0022-0302(06)72191-9","article-title":"Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows","volume":"89","author":"Akers","year":"2006","journal-title":"J. Dairy Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1772","DOI":"10.3168\/jds.2010-3389","article-title":"Early lactation ratio of fat and protein percentage in milk is associated with health, milk production, and survival","volume":"94","author":"Toni","year":"2011","journal-title":"J. Dairy Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/3\/816\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:35:30Z","timestamp":1760160930000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/3\/816"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,14]]},"references-count":33,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["ani11030816"],"URL":"https:\/\/doi.org\/10.3390\/ani11030816","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,14]]}}}