{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:13:58Z","timestamp":1790792038261,"version":"4.1.0"},"reference-count":61,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T00:00:00Z","timestamp":1615852800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The intestinal microbiota is increasingly recognized as an important component of host health, metabolism and immunity. Early gut colonizers are pivotal in the establishment of microbial community structures affecting the health and growth performance of chickens. White Lohmann layer is a common commercial breed. Therefore, this breed was selected to study the pattern of changes of microbiota with age. In this study, the duodenum, caecum and colorectum contents of white Lohmann layer chickens from same environment control farm were collected and analyzed using 16S rRNA sequencing to explore the spatial and temporal variations in intestinal microbiota. The results showed that the diversity of the microbial community structure in the duodenum, caecum and colorectum increased with age and tended to be stable when the layer chickens reached 50 days of age and the distinct succession patterns of the intestinal microbiota between the duodenum and large intestine (caecum and colorectum). On day 0, the diversity of microbes in the duodenum was higher than that in the caecum and colorectum, but the compositions of intestinal microbes were relatively similar, with facultative anaerobic Proteobacteria as the main microbes. However, the relative abundance of facultative anaerobic bacteria (Escherichia) gradually decreased and was replaced by anaerobic bacteria (Bacteroides and Ruminococcaceae). By day 50, the structure of intestinal microbes had gradually become stable, and Lactobacillus was the dominant bacteria in the duodenum (41.1%). The compositions of dominant microbes in the caecum and colorectum were more complex, but there were certain similarities. Bacteroides, Odoribacter and Clostridiales vadin BB60 group were dominant. The results of this study provide evidence that time and spatial factors are important factors affecting the intestinal microbiota composition. This study provides new knowledge of the intestinal microbiota colonization pattern of layer chickens in early life to improve the intestinal health of layer chickens.<\/jats:p>","DOI":"10.3390\/ani11030840","type":"journal-article","created":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T12:01:02Z","timestamp":1615896062000},"page":"840","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Microbial Diversity and Community Variation in the Intestines of Layer Chickens"],"prefix":"10.3390","volume":"11","author":[{"given":"Sha-Sha","family":"Xiao","sequence":"first","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Wushan Road, Tianhe District, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Dui","family":"Mi","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Wushan Road, Tianhe District, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Mei","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Wushan Road, Tianhe District, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6024-0856","authenticated-orcid":false,"given":"Juanboo","family":"Liang","sequence":"additional","affiliation":[{"name":"Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun-Xian","family":"Feng","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Wushan Road, Tianhe District, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin-Bao","family":"Wu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Wushan Road, Tianhe District, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Di","family":"Liao","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Wushan Road, Tianhe District, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Wushan Road, Tianhe District, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"235","DOI":"10.3389\/fmicb.2018.00235","article-title":"Host and Environmental Factors Affecting the Intestinal Microbiota in Chickens","volume":"9","author":"Kers","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Carrasco, J.M.D., Casanova, N.A., and Miyakawa, M.E.F. (2019). Microbiota, Gut Health and Chicken Productivity: What Is the Connection?. Microorganisms, 7.","DOI":"10.3390\/microorganisms7100374"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13059-020-1947-1","article-title":"Assembly of hundreds of novel bacterial genomes from the chicken caecum","volume":"21","author":"Glendinning","year":"2020","journal-title":"Genome Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"254","DOI":"10.3389\/fvets.2018.00254","article-title":"Chicken Gut Microbiota: Importance and Detection Technology","volume":"5","author":"Shang","year":"2018","journal-title":"Front. Vet. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.3389\/fmicb.2017.01967","article-title":"Inheritance and Establishment of Gut Microbiota in Chickens","volume":"8","author":"Jinmei","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1126\/science.aad9378","article-title":"How colonization by microbiota in early life shapes the immune system","volume":"352","author":"Gensollen","year":"2016","journal-title":"Science"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.cell.2014.05.052","article-title":"Altering the intestinal microbiota during a critical developmental window has lasting metabolic consequences","volume":"158","author":"Cox","year":"2014","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1111\/1365-2656.12973","article-title":"Manipulation of gut microbiota during critical developmental windows affects host physiological performance and disease susceptibility across ontogeny","volume":"88","author":"Warne","year":"2019","journal-title":"J. Anim. Ecol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.tree.2015.12.009","article-title":"Evolving Perspectives on Monopolization and Priority Effects","volume":"31","author":"Vanoverbeke","year":"2016","journal-title":"Trends Ecol. Evol."},{"key":"ref_10","first-page":"2","article-title":"Development of the Chick Microbiome: How Early Exposure Influences Future Microbial Diversity","volume":"3","author":"Ballou","year":"2015","journal-title":"Front. Vet. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1111\/j.1574-6976.2008.00161.x","article-title":"Mechanisms of egg contamination by Salmonella Enteritidis","volume":"33","author":"Gantois","year":"2009","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3389\/fvets.2016.00063","article-title":"Applications of In Ovo Technique for the Optimal Development of the Gastrointestinal Tract and the Potential Influence on the Establishment of Its Microbiome in Poultry","volume":"3","author":"Roto","year":"2016","journal-title":"Front. Vet. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e00821","DOI":"10.1002\/mbo3.821","article-title":"Patterns of community assembly in the developing chicken microbiome reveal rapid primary succession","volume":"8","author":"Jurburg","year":"2019","journal-title":"MicrobiologyOpen"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3389\/fvets.2019.00201","article-title":"Development of the Caecal Microbiota in Three Broiler Breeds","volume":"6","author":"Richards","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1079\/WPS200445","article-title":"The role of the commensal gut microbial community in broiler chickens","volume":"61","author":"Lan","year":"2005","journal-title":"World\u2019s Poult. Sci. J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"671","DOI":"10.3382\/ps.2012-02822","article-title":"Bacterial census of poultry intestinal microbiome","volume":"92","author":"Wei","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.1111\/j.1365-2672.2007.03699.x","article-title":"Effects of zinc bacitracin, bird age and access to range on bacterial microbiota in the ileum and caeca of broiler chickens","volume":"104","author":"Gong","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1942","DOI":"10.3382\/ps.2014-03974","article-title":"Spatial heterogeneity and stability of bacterial community in the gastrointestinal tracts of broiler chickens","volume":"93","author":"Choi","year":"2014","journal-title":"Poult. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.giec.2016.08.001","article-title":"Anatomy and Physiology of the Small Bowel","volume":"27","author":"Volk","year":"2017","journal-title":"Gastrointest. Endosc. Clin. N. Am."},{"key":"ref_20","first-page":"1","article-title":"Examination of the temporal and spatial dynamics of the gut microbiome in newborn piglets reveals distinct microbial communities in six intestinal segments","volume":"9","author":"Liu","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"285","DOI":"10.2217\/17460913.2.3.285","article-title":"Microbial communities in the human small intestine: Coupling diversity to metagenomics","volume":"2","author":"Booijink","year":"2007","journal-title":"Future Microbiol."},{"key":"ref_22","first-page":"357","article-title":"Effect of a probiotic on resistance and rearing performance of broiler chickens","volume":"6","author":"Pietras","year":"2000","journal-title":"Rocz. Nauk. Zootech."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2079","DOI":"10.1128\/AEM.64.6.2079-2085.1998","article-title":"Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis","volume":"64","author":"Kerovuo","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2324","DOI":"10.3382\/ps.2009-00250","article-title":"Microbial community composition of the crop and ceca contents of laying hens fed diets supplemented with Chlorella vulgaris","volume":"88","author":"Janczyk","year":"2009","journal-title":"Poult. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Yang, F., Hou, C., Zeng, X., and Qiao, S. (2015). The Use of Lactic Acid Bacteria as a Probiotic in Swine Diets. Pathogens, 4.","DOI":"10.3390\/pathogens4010034"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1099\/00207713-24-2-260","article-title":"New Genus, Coprococcus, Twelve New Species, and Emended Descriptions of Four Previously Described Species of Bacteria from Human Feces","volume":"24","author":"Holdeman","year":"1974","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1093\/jn\/137.4.916","article-title":"Cecal Infusion of Butyrate Increases Intestinal Cell Proliferation in Piglets","volume":"137","author":"Kien","year":"2007","journal-title":"J. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1093\/japr\/14.2.444","article-title":"Comparative Gut Microflora, Metabolic Challenges, and Potential Opportunities","volume":"14","author":"Apajalahti","year":"2005","journal-title":"J. Appl. Poult. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1093\/ps\/84.12.1851","article-title":"Supplementation of coated butyric acid in the feed reduces colonization and shedding of Salmonella in poultry","volume":"84","author":"Boyen","year":"2005","journal-title":"Poult. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Sergeant, M.J., Constantinidou, C., Cogan, T.A., Bedford, M.R., and Pallen, M.J. (2014). Extensive Microbial and Functional Diversity within the Chicken Cecal Microbiome. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0091941"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1080\/00071667208415953","article-title":"The intestinal flora of the chicken in the period 2 to 6 weeks of age, with particular reference to the anaerobic bacteria","volume":"13","author":"Barnes","year":"1972","journal-title":"Br. Poult. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Videnska, P., Sedlar, K., Lukac, M., Faldynova, M., Gerzova, L., Cejkova, D., Sisak, F., and Rychlik, I. (2014). Succession and Replacement of Bacterial Populations in the Caecum of Egg Laying Hens over Their Whole Life. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0115142"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1186\/s40168-017-0315-1","article-title":"Feed-additive probiotics accelerate yet antibiotics delay intestinal microbiota maturation in broiler chicken","volume":"5","author":"Gao","year":"2017","journal-title":"Microbiome"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5112","DOI":"10.1128\/AEM.01043-13","article-title":"Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform","volume":"79","author":"Kozich","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat. Methods"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1038\/nbt.2676","article-title":"Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences","volume":"31","author":"Langille","year":"2013","journal-title":"Nat. Biotechnol. Sci. Bus. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"R60","DOI":"10.1186\/gb-2011-12-6-r60","article-title":"Metagenomic biomarker discovery and explanation","volume":"12","author":"Segata","year":"2011","journal-title":"Genome Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/0092-8674(87)90085-7","article-title":"Protein sorting in yeast: The localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide","volume":"48","author":"Valls","year":"1987","journal-title":"Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2144","DOI":"10.1128\/aem.58.7.2144-2152.1992","article-title":"Purification and characterization of two serine carboxypeptidases from Aspergillus niger and their use in C-terminal sequencing of proteins and peptide synthesis","volume":"58","author":"Ribadeaudumas","year":"1992","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s10142-005-0008-x","article-title":"Expression genetics and haplotype analysis reveal cis regulation of serine carboxypeptidase I (Cxp1), a candidate gene for malting quality in barley (Hordeum vulgare L.)","volume":"6","author":"Potokina","year":"2006","journal-title":"Funct. Integr. Genom."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/0076-6879(94)44020-4","article-title":"Carboxypeptidase C and D","volume":"244","author":"Remington","year":"1994","journal-title":"Methods Enzymol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Simon, K., Verwoolde, M.B., Zhang, J., Smidt, H., de Vries Reilingh, G., Kemp, B., and Lammers, A. (2016). Long-term effects of early life microbiota disturbance on adaptive immunity in laying hens. Poult. Sci., 1543\u20131554.","DOI":"10.3382\/ps\/pew088"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s13099-015-0051-7","article-title":"Deciphering chicken gut microbial dynamics based on high-throughput 16S rRNA metagenomics analyses","volume":"7","author":"Sieo","year":"2015","journal-title":"Gut Pathog."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.3382\/ps.2012-02812","article-title":"Polymerase chain reaction detection of naturally occurring Campylobacter in commercial broiler chicken embryos","volume":"92","author":"Hiett","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.4315\/0362-028.JFP-11-322","article-title":"Evidence for Horizontal and Vertical Transmission in Campylobacter Passage from Hen to Her Progeny","volume":"75","author":"Cox","year":"2012","journal-title":"J. Food Prot."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.3382\/ps\/pex359","article-title":"The gastrointestinal microbiome and its association with the control of pathogens in broiler chicken production: A review","volume":"97","author":"Clavijo","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.14340\/spp.2016.03A0001","article-title":"Laying performance, immunity and digestive health of layer chickens fed diets containing a combination of three probiotics","volume":"1","author":"Inatomi","year":"2016","journal-title":"Sci. Postprint"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.3389\/fmicb.2017.01310","article-title":"Age-Related Variations in Intestinal Microflora of Free-Range and Caged Hens","volume":"8","author":"Yizhe","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1023\/A:1010734717218","article-title":"Time Course of Spontaneous Bacterial Translocation from Gastrointestinal Tract and Its Relationship to Intestinal Microflora in Conventionally Reared Infant Rats","volume":"46","author":"Wenzl","year":"2001","journal-title":"Dig. Dis. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"4891","DOI":"10.1128\/IAI.00484-07","article-title":"Respiration of Escherichia coli in the Mouse Intestine","volume":"75","author":"Jones","year":"2007","journal-title":"Infect. Immun."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"4218","DOI":"10.1128\/IAI.05395-11","article-title":"Anaerobic respiration of Escherichia coli in the mouse intestine","volume":"79","author":"Jones","year":"2011","journal-title":"Infect. Immun."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3654","DOI":"10.3382\/ps\/pex185","article-title":"Effects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying hens","volume":"96","author":"Dong","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.tibtech.2012.08.001","article-title":"Relevance and challenges in modeling human gastric and small intestinal digestion","volume":"30","author":"Guerra","year":"2012","journal-title":"Trends Biotechnol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1016\/j.lwt.2015.03.018","article-title":"Phytase-active lactic acid bacteria from sourdoughs: Isolation and identification","volume":"63","author":"Nuobariene","year":"2015","journal-title":"Lwt Food Sci. Technol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s12915-019-0729-2","article-title":"Maternal milk and fecal microbes guide the spatiotemporal development of mucosa-associated microbiota and barrier function in the porcine neonatal gut","volume":"17","author":"Liu","year":"2019","journal-title":"BMC Biol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Pedroso, A.A., and Lee, M.D. (2014). The Composition and Role of the Microbiota in Chickens, Wageningen Academic.","DOI":"10.3920\/9789086867929_004"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4771","DOI":"10.1111\/1462-2920.13943","article-title":"Untangling the cecal microbiota of feral chickens by culturomic and metagenomic analyses","volume":"19","author":"Ferrario","year":"2017","journal-title":"Environ. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1007\/s00248-016-0870-1","article-title":"Spatial Microbial Composition Along the Gastrointestinal Tract of Captive Attwater\u2019s Prairie Chicken","volume":"73","author":"Zhang","year":"2017","journal-title":"Microb. Ecol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1017\/S0043933909000087","article-title":"Dietary modulation of gut microflora in broiler chickens: A review of the role of six kinds of alternatives to in-feed antibiotics","volume":"65","author":"Yang","year":"2009","journal-title":"Worlds Poult. Sci. J."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1080\/00071667708416332","article-title":"The importance of Lactobacilli in maintaining normal microbial balance in the crop","volume":"18","author":"Fuller","year":"1977","journal-title":"Br. Poult. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/3\/840\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:36:40Z","timestamp":1760161000000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/3\/840"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,16]]},"references-count":61,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["ani11030840"],"URL":"https:\/\/doi.org\/10.3390\/ani11030840","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,16]]}}}