{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T19:35:39Z","timestamp":1792265739873,"version":"4.4.0"},"reference-count":59,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T00:00:00Z","timestamp":1616025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["RTI2018-095183-B-I00 (MCI\/AEI\/FEDER, UE)"],"award-info":[{"award-number":["RTI2018-095183-B-I00 (MCI\/AEI\/FEDER, UE)"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008431","name":"Consejer\u00eda de Educaci\u00f3n, Junta de Castilla y Le\u00f3n","doi-asserted-by":"publisher","award":["LE023P20 (FEDER)"],"award-info":[{"award-number":["LE023P20 (FEDER)"]}],"id":[{"id":"10.13039\/501100008431","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Artificial insemination (AI) with cryopreserved semen is still unreliable for extensive pig industry application. Adding seminal plasma (SP) could improve post-thawing quality, but its suitability could vary. We applied a simple cold-shock test (CST, 5 min at 0 \u00b0C) on neat semen for classifying ejaculates (n = 63) as resistant or sensitive, obtaining two SP pools (CST-resistant: SPr, sensitive: SPs). Subsequently, frozen\/thawed spermatozoa from six boars were incubated (37 \u00b0C) in MR-A\u00ae extender (control), 20% SPr, or 20% SPs, and analyzed at 0, 2, and 4 h. SP improved total and progressive motility, with a higher effect for SPr and STR (p &lt; 0.05), decreasing kinematic parameters VCL and VAP, ALH, and BCF. Sperm viability was unaffected. SP increased apoptotic and membrane disorder ratios, and acrosomal damage, not affecting the chromatin structure (DNA fragmentation and immaturity by SCSA), protamination (CMA3), or disulfide levels (mBBr). However, the proportion of spermatozoa with elevated free thiols (disulfide bridges reduction) significantly increased. Results support a stimulatory role of SP on thawed semen, with additional benefits from SPr. The effect of SP and especially SPr after AI should be tested since CST could be a practical test for selecting suitable ejaculates in AI centers.<\/jats:p>","DOI":"10.3390\/ani11030871","type":"journal-article","created":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T11:41:06Z","timestamp":1616067666000},"page":"871","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cold-Shock Test Is a Practical Method for Selecting Boar Ejaculates Yielding Appropriate Seminal Plasma for Post-Thawing Supplementation"],"prefix":"10.3390","volume":"11","author":[{"given":"Est\u00edbaliz","family":"Lacalle","sequence":"first","affiliation":[{"name":"Institute of Animal Health and Cattle Development (INDEGSAL), Universidad de Le\u00f3n, 24071 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4480-273X","authenticated-orcid":false,"given":"Andrea","family":"N\u00fa\u00f1ez","sequence":"additional","affiliation":[{"name":"Institute of Animal Health and Cattle Development (INDEGSAL), Universidad de Le\u00f3n, 24071 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2223-1788","authenticated-orcid":false,"given":"Estela","family":"Fern\u00e1ndez-Alegre","sequence":"additional","affiliation":[{"name":"Bianor Biotech SL, 24007 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itxaso","family":"Crespo-F\u00e9lez","sequence":"additional","affiliation":[{"name":"Institute of Animal Health and Cattle Development (INDEGSAL), Universidad de Le\u00f3n, 24071 Le\u00f3n, Spain"},{"name":"Topigs Norsvin Espa\u00f1a SLU (AIM Ib\u00e9rica), 24225 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"Institute of Animal Health and Cattle Development (INDEGSAL), Universidad de Le\u00f3n, 24071 Le\u00f3n, Spain"},{"name":"Department of Animal Medicine, Surgery and Anatomy (Animal Medicine and Surgery), Universidad de Le\u00f3n, 24071 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3810-0176","authenticated-orcid":false,"given":"Marta Elena","family":"Alonso","sequence":"additional","affiliation":[{"name":"Department of Animal Production, Faculty of Veterinary, Universidad de Le\u00f3n, 24071 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal","family":"Gonz\u00e1lez-Urdiales","sequence":"additional","affiliation":[{"name":"Topigs Norsvin Espa\u00f1a SLU (AIM Ib\u00e9rica), 24225 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2987-4302","authenticated-orcid":false,"given":"Felipe","family":"Mart\u00ednez-Pastor","sequence":"additional","affiliation":[{"name":"Institute of Animal Health and Cattle Development (INDEGSAL), Universidad de Le\u00f3n, 24071 Le\u00f3n, Spain"},{"name":"Department of Molecular Biology (Cell Biology), Universidad de Le\u00f3n, 24071 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.theriogenology.2015.11.026","article-title":"Will AI in Pigs Become More Efficient?","volume":"86","author":"Roca","year":"2016","journal-title":"Theriogenology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.theriogenology.2019.05.030","article-title":"Application of Preserved Boar Semen for Artificial Insemination: Past, Present and Future Challenges","volume":"137","author":"Waberski","year":"2019","journal-title":"Theriogenology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"69","DOI":"10.21451\/1984-3143-AR895","article-title":"State-of-the-Art of Boar Sperm Preservation in Liquid and Frozen State","volume":"14","author":"Yeste","year":"2017","journal-title":"Anim. 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