{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T08:32:36Z","timestamp":1782894756356,"version":"3.54.5"},"reference-count":37,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,19]],"date-time":"2021-03-19T00:00:00Z","timestamp":1616112000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Ruminants ingest large quantities of clay minerals along with inorganic soil constituents in roughages. The layered structure of clay minerals, however, may adsorb cations and may, thus, interfere with the ruminants\u2019 supply of essential trace metals, such as Zn, Mn, Cu, and Fe. As quantitative knowledge about interactions between clay ingestion and essential trace metal metabolism are largely lacking, this in vitro study focussed on the effect of clay on the solubility of dietary Zn and other bivalent trace metals in the digestive tract of ruminants. Therefore, buffered rumen fluid was used for the simulation of ruminal conditions (RC), acidified rumen fluid (pH 2) was used for abomasal conditions (AC), and duodenal chyme was used for duodenal conditions (DC). These media were added with gradient levels of zinc and incubated at 39 \u00b0C for 24 h in the absence or presence of clay minerals. Soluble Zn, Cu, Mn, and Fe were derived by centrifugation (10,000\u00d7 g) of incubated media, and the supernatants were analysed. Clay depressed the solubility of added Zn in ruminal (65.3% vs. 16.5%), abomasal (97.7% vs. 33.7%), and duodenal conditions (41.3% vs. 21.1%), the results of which were statistically significant (p &lt; 0.001). Moreover, clay reduced dissolved Cu (\u00b5g\/mL) (RC: 0.13 vs. 0.10; AC: 0.16 vs. 0.13; DC: 0.10 vs. 0.08) and Mn (\u00b5g\/mL) (RC: 3.00 vs. 1.80; AC: 5.53 vs. 4.80; DC: 3.18 vs. 1.77) (p &lt; 0.05 in all cases). The presence of clay minerals increased the concentrations of solubilised Fe (\u00b5g\/mL) in abomasal (1.80 vs. 2.86, p &lt; 0.05) and duodenal conditions (1.76 vs. 2.67; p &lt; 0.05). In total, the present in vitro study demonstrates the potential of clay minerals ingested with ruminant feeds for depressing the solubility of dietary Zn, as well as the depression of dietary Cu and Mn along the passage of the digesta from the rumen until the duodenum. Additionally, clay minerals may release Fe into the digesta.<\/jats:p>","DOI":"10.3390\/ani11030877","type":"journal-article","created":{"date-parts":[[2021,3,19]],"date-time":"2021-03-19T11:18:38Z","timestamp":1616152718000},"page":"877","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Clay Minerals Affect the Solubility of Zn and Other Bivalent Cations in the Digestive Tract of Ruminants In Vitro"],"prefix":"10.3390","volume":"11","author":[{"given":"Maria","family":"Schlattl","sequence":"first","affiliation":[{"name":"Chair of Animal Nutrition, TUM School of Life Sciences, Technical University of Munich, Liesel-Beckmann-Str. 2, 85354 Freising, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzell","family":"Buffler","sequence":"additional","affiliation":[{"name":"Chair of Animal Nutrition, TUM School of Life Sciences, Technical University of Munich, Liesel-Beckmann-Str. 2, 85354 Freising, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5714-2702","authenticated-orcid":false,"given":"Wilhelm","family":"Windisch","sequence":"additional","affiliation":[{"name":"Chair of Animal Nutrition, TUM School of Life Sciences, Technical University of Munich, Liesel-Beckmann-Str. 2, 85354 Freising, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,19]]},"reference":[{"key":"ref_1","unstructured":"Gerighausen, H.-G. (2011). Ziele in der Wiederk\u00e4uerf\u00fctterung. Praxishandbuch Futter- und Substratkonservierung, DLG-Verlag."},{"key":"ref_2","unstructured":"Nussbaum, H. (2020, February 05). Dreck Macht Nicht Fett\u2014Verschmutzung bei Grassilage. Available online: https:\/\/lazbw.landwirtschaft-bw.de\/pb\/site\/pbs-bw-new\/get\/documents\/MLR.LEL\/PB5Documents\/lazbw_2017\/lazbw_gl\/Gr%C3%BCnlandwirtschaft_und_Futterbau\/Futterkonservierung\/Hinweise%20zum%20Siliermanagement\/Dokumente_Siliermanagement\/2007_GL_nussbaum_verschmutzung_grassilage.pdf?attachment=true."},{"key":"ref_3","unstructured":"(2015). Challenges in silage production with regard to soil contamination. 42. Viehwirtschaftliche Fachtagung 2015. 42. Viehwirtschaftliche Fachtagung 2015, LFZ Raumberg-Gumpenstein."},{"key":"ref_4","unstructured":"(2014). Feedstuff-dirtying of grass-silage of Austrian farms. 19. Alpenl\u00e4ndische Expertenforum, LFZ Raumberg-Gumpenstein."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1017\/S1751731111002734","article-title":"Soil intake of lactating dairy cows in intensive strip grazing systems","volume":"6","author":"Jurjanz","year":"2012","journal-title":"Animal"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"611","DOI":"10.3168\/jds.S0022-0302(82)82238-8","article-title":"Soil ingestion by dairy cattle","volume":"65","author":"Fries","year":"1982","journal-title":"J. Dairy Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S0048-9697(83)80026-6","article-title":"Soil ingestion\u2014A major pathway of heavy metals into livestock grazing contaminated land","volume":"28","author":"Thornton","year":"1983","journal-title":"Sci. Total Environ."},{"key":"ref_8","unstructured":"Scheffer, F., Schachtschabel, P., Blume, H.-P., Br\u00fcmmer, G.W., Horn, R., Kandeler, E., K\u00f6gel-Knabner, I., Kretzschmar, R., Stahr, K., and Thiele-Bruhn, S. (2010). Lehrbuch der Bodenkunde, Spektrum Akademischer Verlag. [16th ed.]."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Sparks, D.L. (2003). Environmental Soil Chemistry, Elsevier.","DOI":"10.1016\/B978-012656446-4\/50001-3"},{"key":"ref_10","unstructured":"Sposito, G. (2008). The Chemistry of Soils, Oxford University Press."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Brigatti, M.F., Galan, E., and Theng, B.K. (2013). Structure and mineralogy of clay minerals. Developments in Clay Science, Elsevier.","DOI":"10.1016\/B978-0-08-098258-8.00002-X"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.chemosphere.2006.12.062","article-title":"Monometal and competitive adsorption of heavy metals by sewage sludge-amended soil","volume":"68","author":"Antoniadis","year":"2007","journal-title":"Chemosphere"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.cis.2013.10.005","article-title":"A review of the distribution coefficients of trace elements in soils: Influence of sorption system, element characteristics, and soil colloidal properties","volume":"201","author":"Shaheen","year":"2013","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Alloway, B.J. (1995). Soil processes and the behaviour of metals. Heavy Metals in Soils, Blackie Academic & Professional. [2nd ed.].","DOI":"10.1007\/978-94-011-1344-1"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1097\/00010694-199204000-00010","article-title":"Effect of cropping systems on adsorption of metals by soils: III. competitive adsorption1","volume":"153","author":"Basta","year":"1992","journal-title":"Soil Sci."},{"key":"ref_16","first-page":"117","article-title":"Simultaneous adsorption of cadmium, zinc, and lead on hydroxyaluminum-and hydroxyaluminosilicate-montmorillonite complexes","volume":"66","author":"Saha","year":"2002","journal-title":"Soil Sci. Soc. Am. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.envpol.2005.07.005","article-title":"Effect of the cationic composition of sorption solution on the quantification of sorption\u2013desorption parameters of heavy metals in soils","volume":"140","author":"Sastre","year":"2006","journal-title":"Environ. Pollut."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1139\/t93-073","article-title":"pH influence on selectivity and retention of heavy metals in some clay soils","volume":"30","author":"Yong","year":"1993","journal-title":"Can. Geotech. J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1023\/A:1025401927023","article-title":"Comparison of heavy metal adsorptions by Thai kaolin and ballclay","volume":"148","author":"Chantawong","year":"2003","journal-title":"Water Air Soil Pollut."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1002\/(SICI)1097-4660(199912)74:12<1131::AID-JCTB158>3.0.CO;2-0","article-title":"Effect of pH, flow rate and concentration on the sorption of Pb and Cd on montmorillonite: I. Experimental","volume":"74","author":"Altin","year":"1999","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.jhazmat.2007.04.109","article-title":"Adsorption of copper and zinc from aqueous solutions by using natural clay","volume":"149","author":"Veli","year":"2007","journal-title":"J. Hazard. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.desal.2010.09.041","article-title":"Adsorption of zinc (Zn2+) from aqueous solution on natural bentonite","volume":"267","author":"Sen","year":"2011","journal-title":"Desalination"},{"key":"ref_23","unstructured":"McBride, M.B. (1994). Environmental Chemistry of Soils, Oxford Univ Press."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"von Engelhardt, W., Breves, G., Diener, M., and G\u00e4bel, G. (2015). Physiologie der Haustiere, Georg Thieme Verlag.","DOI":"10.1055\/b-003-125805"},{"key":"ref_25","first-page":"7","article-title":"Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid","volume":"28","author":"Menke","year":"1988","journal-title":"Anim. Res. Dev."},{"key":"ref_26","unstructured":"Schroeder, D. (1992). Bodenkunde in Stichworten, Ferdinand Hirt. [5th ed.]."},{"key":"ref_27","unstructured":"DLG (2020, February 27). Feed Value Database. Available online: http:\/\/datenbank.futtermittel.net."},{"key":"ref_28","unstructured":"GfE (2001). Empfehlungen zur Energie- und N\u00e4hrstoffversorgung der Milchk\u00fche und Aufzuchtrinder, DLG-Verl."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S1387-1811(03)00360-3","article-title":"Sorption characteristics of zinc and iron by natural zeolite and bentonite","volume":"61","author":"Sheta","year":"2003","journal-title":"Microporous Mesoporous Mater."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"496","DOI":"10.2527\/jas1969.293496x","article-title":"Influence of graded levels of dietary iron, as ferrous sulfate, on performance and tissue mineral composition of steers","volume":"29","author":"Standish","year":"1969","journal-title":"J. Anim. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1017\/S0003356100002890","article-title":"Iron-induced copper deficiency in calves: Dose-response relationships and interactions with molybdenum and sulphur","volume":"45","author":"Bremner","year":"1987","journal-title":"Anim. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1093\/jn\/122.6.1300","article-title":"Varying levels of manganese and iron affect absorption and gut endogenous losses of manganese by rats","volume":"122","author":"Davis","year":"1992","journal-title":"J. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.livprodsci.2004.05.001","article-title":"Bioavailability of zinc from zinc sulfate and different organic zinc sources and their effects on ruminal volatile fatty acid proportions","volume":"90","author":"Spears","year":"2004","journal-title":"Livest. Prod. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"566","DOI":"10.3168\/jds.2014-8624","article-title":"The effect of trace mineral source and concentration on ruminal digestion and mineral solubility","volume":"98","author":"Genther","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.2527\/1993.7151281x","article-title":"Ruminal distribution of zinc in steers fed a polysaccharide-zinc complex or zinc oxide","volume":"71","author":"Kennedy","year":"1993","journal-title":"J. Anim. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S0162-0134(99)00041-0","article-title":"31P NMR, potentiometric and spectrophotometric studies of phytic acid ionization and complexation properties toward Co2+, Ni2+, Cu2+, Zn2+ and Cd2+","volume":"75","author":"Dange","year":"1999","journal-title":"J. Inorg. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.jcis.2005.07.073","article-title":"Adsorption of Ni (II) on clays","volume":"295","author":"Gupta","year":"2006","journal-title":"J. Colloid Interface Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/3\/877\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:38:00Z","timestamp":1760161080000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/3\/877"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,19]]},"references-count":37,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["ani11030877"],"URL":"https:\/\/doi.org\/10.3390\/ani11030877","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,19]]}}}