{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:19:11Z","timestamp":1790266751702,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T00:00:00Z","timestamp":1618444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministerio de Ciencia e Innovaci\u00f3n y Universidades","award":["RTI2018-098711-B-I00"],"award-info":[{"award-number":["RTI2018-098711-B-I00"]}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["RTI2018-098711-B-I00"],"award-info":[{"award-number":["RTI2018-098711-B-I00"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["RTI2018-098711-B-I00"],"award-info":[{"award-number":["RTI2018-098711-B-I00"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["REDPRION"],"award-info":[{"award-number":["REDPRION"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Scrapie is a prion disease affecting sheep and goats and it is considered a prototype of transmissible spongiform encephalopathies (TSEs). Mesenchymal stem cells (MSCs) have been proposed as candidates for developing in vitro models of prion diseases. Murine MSCs are able to propagate prions after previous mouse-adaptation of prion strains and, although ovine MSCs express the cellular prion protein (PrPC), their susceptibility to prion infection has never been investigated. Here, we analyze the potential of ovine bone marrow-derived MSCs (oBM-MSCs), in growth and neurogenic conditions, to be infected by natural scrapie and propagate prion particles (PrPSc) in vitro, as well as the effect of this infection on cell viability and proliferation. Cultures were kept for 48\u201372 h in contact with homogenates of central nervous system (CNS) samples from scrapie or control sheep. In growth conditions, oBM-MSCs initially maintained detectable levels of PrPSc post-inoculation, as determined by Western blotting and ELISA. However, the PrPSc signal weakened and was lost over time. oBM-MSCs infected with scrapie displayed lower cell doubling and higher doubling times than those infected with control inocula. On the other hand, in neurogenic conditions, oBM-MSCs not only maintained detectable levels of PrPSc post-inoculation, as determined by ELISA, but this PrPSc signal also increased progressively over time. Finally, inoculation with CNS extracts seems to induce the proliferation of oBM-MSCs in both growth and neurogenic conditions. Our results suggest that oBM-MSCs respond to prion infection by decreasing their proliferation capacity and thus might not be permissive to prion replication, whereas ovine MSC-derived neuron-like cells seem to maintain and replicate PrPSc.<\/jats:p>","DOI":"10.3390\/ani11041137","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T21:35:13Z","timestamp":1618522513000},"page":"1137","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effect of Scrapie Prion Infection in Ovine Bone Marrow-Derived Mesenchymal Stem Cells and Ovine Mesenchymal Stem Cell-Derived Neurons"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2954-1068","authenticated-orcid":false,"given":"Laura","family":"Garc\u00eda-Mend\u00edvil","sequence":"first","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Bioqu\u00edmica (LAGENBIO), Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"},{"name":"Biomedical Signal Interpretation and Computational Simulation (BSICoS), Institute of Engineering Research (I3A), University of Zaragoza &amp; Instituto de Investigaci\u00f3n Sanitaria (IIS), 50018 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego R.","family":"Mediano","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Bioqu\u00edmica (LAGENBIO), Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6806-9990","authenticated-orcid":false,"given":"Adelaida","family":"Hernaiz","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Bioqu\u00edmica (LAGENBIO), Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5228-248X","authenticated-orcid":false,"given":"David","family":"Sanz-Rubio","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Bioqu\u00edmica (LAGENBIO), Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"},{"name":"Translational Research Unit, Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Hospital Universitario Miguel Servet, 50009 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8712-2275","authenticated-orcid":false,"given":"Francisco J.","family":"V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Bioqu\u00edmica (LAGENBIO), Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"},{"name":"Departamento de Patolog\u00eda Animal, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bel\u00e9n","family":"Mar\u00edn","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Encefalopat\u00edas y Enfermedades Transmisibles Emergentes, Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d3scar","family":"L\u00f3pez-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Bioqu\u00edmica (LAGENBIO), Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"},{"name":"Centro de Investigaci\u00f3n en Encefalopat\u00edas y Enfermedades Transmisibles Emergentes, Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Neurodegenerativas (CIBERNED), Instituto Carlos III, 28031 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9075-2764","authenticated-orcid":false,"given":"Alicia","family":"Otero","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Encefalopat\u00edas y Enfermedades Transmisibles Emergentes, Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan J.","family":"Badiola","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Encefalopat\u00edas y Enfermedades Transmisibles Emergentes, Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Zaragoza","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Bioqu\u00edmica (LAGENBIO), Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Neurodegenerativas (CIBERNED), Instituto Carlos III, 28031 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ordov\u00e1s","sequence":"additional","affiliation":[{"name":"Biomedical Signal Interpretation and Computational Simulation (BSICoS), Institute of Engineering Research (I3A), University of Zaragoza &amp; Instituto de Investigaci\u00f3n Sanitaria (IIS), 50018 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Bolea","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Encefalopat\u00edas y Enfermedades Transmisibles Emergentes, Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6016-4726","authenticated-orcid":false,"given":"Inmaculada","family":"Mart\u00edn-Burriel","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Bioqu\u00edmica (LAGENBIO), Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"},{"name":"Centro de Investigaci\u00f3n en Encefalopat\u00edas y Enfermedades Transmisibles Emergentes, Instituto Agroalimentario de Arag\u00f3n (IA2), Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n (IISArag\u00f3n), Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Neurodegenerativas (CIBERNED), Instituto Carlos III, 28031 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1111\/j.1750-3639.1998.tb00171.x","article-title":"The prion diseases","volume":"8","author":"Prusiner","year":"1998","journal-title":"Brain Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1126\/science.6801762","article-title":"Novel proteinaceous infectious particles cause scrapie","volume":"216","author":"Prusiner","year":"1982","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1093\/oxfordjournals.bmb.a072645","article-title":"Neuropathology of spongiform encephalopathies in humans","volume":"49","author":"Bell","year":"1993","journal-title":"Br. Med. Bull."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/S0034-5288(18)34630-7","article-title":"The astrocytic reaction in experimental scrapie in the rat","volume":"8","author":"Pattison","year":"1967","journal-title":"Res. Vet. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zabel, M.D., and Reid, C. (2015). A brief history of prions. Pathog. Dis., 73.","DOI":"10.1093\/femspd\/ftv087"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/978-1-4939-7244-9_20","article-title":"Cell culture methods for screening of prion therapeutics","volume":"Volume 1658","author":"McMahon","year":"2017","journal-title":"Methods in Molecular Biology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-59745-234-2_1","article-title":"Cell culture models to unravel prion protein function and aberrancies in prion diseases","volume":"459","author":"Bedecs","year":"2008","journal-title":"Methods Mol. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1093\/bmb\/66.1.87","article-title":"Prion propagation in cultured cells","volume":"66","author":"Solassol","year":"2003","journal-title":"Br. Med. Bull."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1126\/science.284.5411.143","article-title":"Multilineage potential of adult human mesenchymal stem cells","volume":"284","author":"Pittenger","year":"1999","journal-title":"Science"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1002\/jnr.10365","article-title":"Adult bone marrow stromal stem cells express germline, ectodermal, endodermal, and mesodermal genes prior to neurogenesis","volume":"69","author":"Woodbury","year":"2002","journal-title":"J. Neurosci. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1006\/exnr.2001.7853","article-title":"Human bone marrow stem cells exhibit neural phenotypes and ameliorate neurological deficits after grafting into the ischemic brain of rats","volume":"174","author":"Zhao","year":"2002","journal-title":"Exp. Neurol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/j.bbrc.2008.10.099","article-title":"Bone marrow stroma cells are susceptible to prion infection","volume":"377","author":"Takakura","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1007\/s12031-016-0733-8","article-title":"Cellular Prion Protein Promotes Neuronal Differentiation of Adipose-Derived Stem Cells by Upregulating miRNA-124","volume":"59","author":"Shi","year":"2016","journal-title":"J. Mol. Neurosci."},{"key":"ref_14","first-page":"e59282","article-title":"Isolation, propagation, and prion protein expression during neuronal differentiation of human dental pulp stem cells","volume":"2019","author":"Martellucci","year":"2019","journal-title":"J. Vis. Exp."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Martellucci, S., Santacroce, C., Santilli, F., Piccoli, L., Monache, S.D., Angelucci, A., Misasi, R., Sorice, M., and Mattei, V. (2019). Cellular and molecular mechanisms mediated by recPrP C involved in the neuronal differentiation process of mesenchymal stem cells. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20020345"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2615","DOI":"10.1099\/jgv.0.000907","article-title":"Therapeutic effect of autologous compact bone-derived mesenchymal stem cell transplantation on prion disease","volume":"98","author":"Shan","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_17","first-page":"205","article-title":"Murine bone marrow stromal cell culture with features of mesenchymal stem cells susceptible to mouse-adapted human TSE agent, Fukuoka-1","volume":"47","author":"Akimov","year":"2009","journal-title":"Folia Neuropathol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3715","DOI":"10.1099\/jgv.0.000292","article-title":"Characterization of mesenchymal stem cells in sheep naturally infected with scrapie","volume":"96","author":"Mediano","year":"2015","journal-title":"J. Gen. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1186\/s40478-019-0742-2","article-title":"Sporadic Creutzfeldt-Jakob disease prion infection of human cerebral organoids","volume":"7","author":"Groveman","year":"2019","journal-title":"Acta Neuropathol. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3481","DOI":"10.1084\/jem.20161547","article-title":"Human stem cell-derived astrocytes replicate human prions in a PRNP genotype-dependent manner","volume":"214","author":"Krejciova","year":"2017","journal-title":"J. Exp. Med."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Lyahyai, J., Mediano, D.R., Ranera, B., Sanz, A., Remacha, A.R., Bolea, R., Zaragoza, P., Rodellar, C., and Mart\u00edn-Burriel, I. (2012). Isolation and characterization of ovine mesenchymal stem cells derived from peripheral blood. BMC Vet. Res., 8.","DOI":"10.1186\/1746-6148-8-169"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.brainres.2006.06.021","article-title":"Pathological findings in retina and visual pathways associated to natural Scrapie in sheep","volume":"1108","author":"Hortells","year":"2006","journal-title":"Brain Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3254","DOI":"10.1128\/JCM.38.9.3254-3259.2000","article-title":"Preclinical diagnosis of scrapie by immunohistochemistry of third eyelid lymphoid tissue","volume":"38","author":"Baszler","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1111\/j.2042-3306.2010.00353.x","article-title":"Comparative study of equine bone marrow and adipose tissue-derived mesenchymal stromal cells","volume":"44","author":"Ranera","year":"2012","journal-title":"Equine Vet. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1080\/14653240600855905","article-title":"Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement","volume":"8","author":"Dominici","year":"2006","journal-title":"Cytotherapy"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1177\/104063870501700511","article-title":"Comparison of immunohistochemistry and two rapid tests for detection of abnormal prion protein in different brain regions of sheep with typical scrapie","volume":"17","author":"Bolea","year":"2005","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1297-9716-44-14","article-title":"Gene and protein patterns of potential prion-related markers in the central nervous system of clinical and preclinical infected sheep","volume":"44","author":"Filali","year":"2013","journal-title":"Vet. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1186\/ar130","article-title":"Mesenchymal precursor cells in the blood of normal individuals","volume":"2","author":"Zvaifler","year":"2000","journal-title":"Arthritis Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1006\/exnr.2000.7389","article-title":"Adult bone marrow stromal cells differentiate into neural cells in vitro","volume":"164","author":"Song","year":"2000","journal-title":"Exp. Neurol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"10959","DOI":"10.1128\/JVI.01085-08","article-title":"Persistent Propagation of Variant Creutzfeldt-Jakob Disease Agent in Murine Spleen Stromal Cell Culture with Features of Mesenchymal Stem Cells","volume":"82","author":"Akimov","year":"2008","journal-title":"J. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1111\/j.1537-2995.2010.03041.x","article-title":"Fukuoka-1 strain of transmissible spongiform encephalopathy agent infects murine bone marrow-derived cells with features of mesenchymal stem cells","volume":"51","author":"Cervenakova","year":"2011","journal-title":"Transfusion"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5918","DOI":"10.1128\/JVI.00165-09","article-title":"Effect of Transplantation of Bone Marrow-Derived Mesenchymal Stem Cells on Mice Infected with Prions","volume":"83","author":"Song","year":"2009","journal-title":"J. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/S1474-4422(02)00040-6","article-title":"Treatment of neural injury with marrow stromal cells","volume":"1","author":"Chopp","year":"2002","journal-title":"Lancet Neurol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"11069","DOI":"10.1128\/JVI.05318-11","article-title":"Identification of Chemoattractive Factors Involved in the Migration of Bone Marrow-Derived Mesenchymal Stem Cells to Brain Lesions Caused by Prions","volume":"85","author":"Song","year":"2011","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Bonab, M.M., Alimoghaddam, K., Talebian, F., Ghaffari, S.H., Ghavamzadeh, A., and Nikbin, B. (2006). Aging of mesenchymal stem cell in vitro. BMC Cell Biol., 7.","DOI":"10.1186\/1471-2121-7-14"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.jcyt.2013.07.010","article-title":"Mesenchymal stromal cells from unconventional model organisms","volume":"16","author":"Calloni","year":"2014","journal-title":"Cytotherapy"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"17971","DOI":"10.1073\/pnas.0708372104","article-title":"Cell division modulates prion accumulation in cultured cells","volume":"104","author":"Ghaemmaghami","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/S0960-9822(02)00722-4","article-title":"Transfer of scrapie prion infectivity by cell contact in culture","volume":"12","author":"Kanu","year":"2002","journal-title":"Curr. Biol."},{"key":"ref_39","unstructured":"Mediano, D.R. (2016). Caracterizaci\u00f3n de C\u00e9lulas Madre Mesenquimales Ovinas Infectadas Por Scrapie, University of Zaragoza. (In Spanish)."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/4\/1137\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:48:33Z","timestamp":1760161713000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/4\/1137"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,15]]},"references-count":39,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4]]}},"alternative-id":["ani11041137"],"URL":"https:\/\/doi.org\/10.3390\/ani11041137","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,15]]}}}