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Despite the fact that sperm sex-sorting is one of the most intense studied technologies and notable progress have been made in the past three decades to optimize it, the conception rates when using sex-sorted semen are still under expectations. Assisted reproduction programs may benefit from the use of emergent nano and microfluidic-based technologies. This article addresses the currently used methods for sperm sex-sorting, as well as the emerging ones, based on nanotechnology and microfluidics emphasizing on their practical and economic applicability.<\/jats:p>","DOI":"10.3390\/ani11041182","type":"journal-article","created":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T13:58:04Z","timestamp":1618927084000},"page":"1182","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Game-Changing Approaches in Sperm Sex-Sorting: Microfluidics and Nanotechnology"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6022-583X","authenticated-orcid":false,"given":"Andra-Sabina","family":"Neculai-Valeanu","sequence":"first","affiliation":[{"name":"Research and Development Station for Cattle Breeding Dancu, 707252 Iasi, Romania"},{"name":"Department of Fundamental Sciences in Animal Husbandry, Faculty of Food and Animal Sciences, University of Applied Life Sciences and Environment \u201cIon Ionescu de la Brad\u201d, 700490 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adina Mirela","family":"Ariton","sequence":"additional","affiliation":[{"name":"Research and Development Station for Cattle Breeding Dancu, 707252 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Fr\u00f3na, D., Szender\u00e1k, J., and Harangi-R\u00e1kos, M. 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