{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T19:27:45Z","timestamp":1771529265930,"version":"3.50.1"},"reference-count":51,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,5,23]],"date-time":"2021-05-23T00:00:00Z","timestamp":1621728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Laboratorios Syva S.A.U.","award":["Z258-00067\/001"],"award-info":[{"award-number":["Z258-00067\/001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Flumequine concentrations in plasma, colon tissue and intestinal contents were evaluated in 12 healthy pigs after oral administration (12 mg\/kg every 24 h for 5 consecutive days in drinking water). Plasma, colon tissue and intestinal content samples were collected from animals sacrificed on days 3, 6 and 7. Concentrations were measured by high performance liquid chromatography after having validated the method, following the European Medicines Agency (EMA) requirements. The drug was not detected in any plasma sample. In colon tissue, concentrations were higher on day 3 (0.230 \u00b1 0.033 \u00b5g\/g, descending colon; 0.156 \u00b1 0.093 \u00b5g\/g, ascending colon) than on day 6 (0.187 \u00b1 0.123 \u00b5g\/g, descending colon; 0.107 \u00b1 0.007 \u00b5g\/g, ascending colon). Concentrations were considerably higher in intestinal contents, again on day 3 (1.349 \u00b1 1.401 \u00b5g\/g, descending colon; 0.591 \u00b1 0.209 \u00b5g\/g, ascending colon) than on days 6 (0.979 \u00b1 0.346 \u00b5g\/g, descending colon; 0.595 \u00b1 0.075 \u00b5g\/g, ascending colon) and 7 (0.247 \u00b1 0.172 \u00b5g\/g, descending colon; 0.172 \u00b1 0.086 \u00b5g\/g, ascending colon). Measured concentrations were lower than those effective against the most common intestinal pathogenic microorganisms in swine and, more specifically, Brachyspira hyodysenteriae.<\/jats:p>","DOI":"10.3390\/ani11061514","type":"journal-article","created":{"date-parts":[[2021,5,23]],"date-time":"2021-05-23T23:58:05Z","timestamp":1621814285000},"page":"1514","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Distribution of Flumequine in Intestinal Contents and Colon Tissue in Pigs after Its Therapeutic Use in the Drinking Water"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9463-1628","authenticated-orcid":false,"given":"Jose M.","family":"Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Pharmacology, Department of Biomedical Sciences, Institute of Biomedicine (IBIOMED), Veterinary Faculty, University of Leon, 24071 Leon, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2101-3906","authenticated-orcid":false,"given":"M. Jose","family":"Diez","sequence":"additional","affiliation":[{"name":"Pharmacology, Department of Biomedical Sciences, Institute of Biomedicine (IBIOMED), Veterinary Faculty, University of Leon, 24071 Leon, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0208-9542","authenticated-orcid":false,"given":"Matilde","family":"Sierra","sequence":"additional","affiliation":[{"name":"Pharmacology, Department of Biomedical Sciences, Institute of Biomedicine (IBIOMED), Veterinary Faculty, University of Leon, 24071 Leon, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7821-9502","authenticated-orcid":false,"given":"Juan J.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Pharmacology, Department of Biomedical Sciences, Institute of Biomedicine (IBIOMED), Veterinary Faculty, University of Leon, 24071 Leon, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1225-4097","authenticated-orcid":false,"given":"Nelida","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Pharmacology, Department of Biomedical Sciences, Institute of Biomedicine (IBIOMED), Veterinary Faculty, University of Leon, 24071 Leon, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3729-5431","authenticated-orcid":false,"given":"Raquel","family":"Diez","sequence":"additional","affiliation":[{"name":"Pharmacology, Department of Biomedical Sciences, Institute of Biomedicine (IBIOMED), Veterinary Faculty, University of Leon, 24071 Leon, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3311-4226","authenticated-orcid":false,"given":"Ana M.","family":"Sahagun","sequence":"additional","affiliation":[{"name":"Pharmacology, Department of Biomedical Sciences, Institute of Biomedicine (IBIOMED), Veterinary Faculty, University of Leon, 24071 Leon, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1093\/oxfordjournals.jac.a020889","article-title":"Quinolone generations: Natural history or natural selection?","volume":"46","author":"Ball","year":"2000","journal-title":"J. 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