{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T23:47:48Z","timestamp":1768693668310,"version":"3.49.0"},"reference-count":54,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T00:00:00Z","timestamp":1623024000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Agriculture and Rural Development of Poland","award":["-"],"award-info":[{"award-number":["-"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The study investigated the effect of replacing soybean meal with extruded faba bean seeds on piglet growth performance and selected microbial activity indices in the large intestine. In total, 24, 35-day-old, healthy, castrated piglets of similar body weight were divided into four groups with six replicates. Animals in the control group (C) were fed with soybean meal without extruded faba bean seeds. In other experimental groups, pigs were fed diets with the addition of 20 (FB20), 25 (FB25) or 30% (FB30) extruded faba bean seeds instead of soybean meal and wheat starch. Growth performance, histology of the large intestine, short-chain fatty acids (SCFA) and ammonia concentration, as well as the activity of bacterial enzymes in digesta samples, were analyzed. The intake of the FB25 diet resulted in an increased feed:gain ratio in comparison to the FB30 group. Feeding the FB30 diet increased tunica muscularis thickness in the caecum as compared to other groups. Moreover, dietary inclusion of extruded faba bean seeds had no effect on SCFA and ammonia concentration. In addition, feeding diets with a different level of extruded faba bean seeds did not affect the activity of bacterial enzymes in the colon.<\/jats:p>","DOI":"10.3390\/ani11061703","type":"journal-article","created":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T13:25:43Z","timestamp":1623072343000},"page":"1703","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The Effect of a Diet Containing Extruded Faba Bean Seeds on Growth Performance and Selected Microbial Activity Indices in the Large Intestine of Piglets"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7480-3592","authenticated-orcid":false,"given":"Anna","family":"Tu\u015bnio","sequence":"first","affiliation":[{"name":"Department of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jab\u0142onna, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3428-9805","authenticated-orcid":false,"given":"Marcin","family":"Barszcz","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jab\u0142onna, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8963-9745","authenticated-orcid":false,"given":"Marcin","family":"Taciak","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jab\u0142onna, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"\u015awi\u0119ch","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jab\u0142onna, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5981-6109","authenticated-orcid":false,"given":"Agnieszka","family":"W\u00f3jtowicz","sequence":"additional","affiliation":[{"name":"Department of Thermal Technology and Food Process Engineering, University of Life Sciences in Lublin, G\u0142\u0119boka 31, 20-612 Lublin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacek","family":"Skomia\u0142","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jab\u0142onna, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1080\/1745039X.2013.801137","article-title":"Influence of partial replacement of soya bean meal by faba beans or peas in heavy pigs diet on meat quality, residual anti-nutritional factors and phytoestrogen content","volume":"67","author":"Gatta","year":"2013","journal-title":"Arch. Anim. Nutr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0378-4290(97)00025-7","article-title":"Faba bean (Vicia faba L.)","volume":"53","author":"Duc","year":"1997","journal-title":"Field Crops Res."},{"key":"ref_3","first-page":"263","article-title":"The effect of partial replacement of soybean meal with fermented soybean meal on chicken performance and immune status","volume":"28","author":"Chachaj","year":"2019","journal-title":"J. Anim. Feed Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1071\/AR00081","article-title":"Dietary lupins (Lupinus angustifolius and Lupinus albus) can increase digesta retention in the gastrointestinal tract of pigs","volume":"52","author":"Dunshea","year":"2001","journal-title":"Austr. J. Agric. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0301-6226(03)00052-6","article-title":"Determining the source of anti-nutritional factor(s) found in two species of lupin (L. albus and L. angustifolius) fed to growing pigs","volume":"84","author":"Ferguson","year":"2003","journal-title":"Liv. Prod. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"521","DOI":"10.3382\/ps.2007-00113","article-title":"Effects of a natural extract of chestnut wood on digestibility, performance traits, and nitrogen balance of broiler chicks","volume":"87","author":"Schiavone","year":"2008","journal-title":"Poult. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1080\/17450390903461584","article-title":"Effect of tannins on growth performance and intestinal ecosystem in weaned piglets","volume":"64","author":"Biagia","year":"2010","journal-title":"Arch. Anim. Nutr."},{"key":"ref_8","first-page":"841","article-title":"Effect of chestnut (Castanea sativa Mill.) wood tannins and organic acids on growth performance and faecal microbiota of pigs from 23 to 127 days of age","volume":"19","author":"Brus","year":"2013","journal-title":"Bulg. J. Agric. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0377-8401(01)00302-9","article-title":"The effect of extrusion cooking on mineral bioavailability in pea and kidney bean seed meals","volume":"94","author":"Alonso","year":"2001","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"169","DOI":"10.22358\/jafs\/67957\/2001","article-title":"Effects of raw or extruded legume seeds on some functional and morphological gut parameters in rats","volume":"10","author":"Leontowicz","year":"2001","journal-title":"J. Anim. Feed Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.jfoodeng.2004.03.001","article-title":"Modelling macromolecular degradation of corn starch in a twin screw extruder","volume":"66","author":"Bosman","year":"2005","journal-title":"J. Food Eng."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.livsci.2018.03.017","article-title":"Variations in nutritional and antinutritional contents among faba bean cultivars and effects on growth performance of weaner pigs","volume":"212","author":"Ivarsson","year":"2018","journal-title":"Liv. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.aninu.2017.09.004","article-title":"Potential and challenges of tannins as an alternative to in-feed antibiotics for farm animal production","volume":"4","author":"Huang","year":"2018","journal-title":"Anim. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1051\/animres:2004019","article-title":"Microbial diversity studies of the porcine gastrointestinal ecosystem during weaning transition","volume":"53","author":"Konstantinov","year":"2004","journal-title":"Anim. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1051\/animres:2004018","article-title":"Gut function and dysfunction in young pigs: Physiology","volume":"53","author":"Boudry","year":"2004","journal-title":"Anim. Res."},{"key":"ref_16","unstructured":"Garnsworthy, P.C., and Wiseman, J. (1999). Utilisation of different fibres in piglet feeds. Recent Advances in Animal Nutrition, Nottingham University Press."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3821","DOI":"10.1128\/AEM.70.7.3821-3830.2004","article-title":"Specific response of a novel and abundant Lactobacillus amylovorus-like phylotype to dietary prebiotics in the guts of weaning piglets","volume":"70","author":"Konstantinov","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Gibson, G.R., and Roberfroid, M.B. (1999). The human colonic microbiota. Colonic Microbiota, Nutrition and Health, Kluwer Academic Publishers.","DOI":"10.1007\/978-94-017-1079-4"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1111\/j.1439-0396.2007.00736.x","article-title":"Effects of differentially fermentable carbohydrates on the microbial fermentation profile of the gastrointestinal tract of broilers","volume":"92","author":"Rehman","year":"2008","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1097\/00004836-200603000-00015","article-title":"Colonic health: Fermentation and short chain fatty acids","volume":"40","author":"Wong","year":"2006","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_21","first-page":"1647","article-title":"Protein degradation by human intestinal bacteria","volume":"132","author":"MacFarlane","year":"1986","journal-title":"J. Gen. Microbiol."},{"key":"ref_22","unstructured":"Piva, A., Bach Knudsen, K.E., and Lindberg, J.E. (2001). Possible ways of modifying type and amount of products from microbial fermentation in the gut. Gut Environment of Pigs, Nottingham University Press."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1080\/01635580701649644","article-title":"Effect of colonic bacterial metabolites on Caco-2 cell paracellular permeability in vitro","volume":"60","author":"Hughes","year":"2008","journal-title":"Nutr. Cancer"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1002\/ptr.1331","article-title":"Fenugreek affects the activity of \u03b2-glucuronidase and mucinase in the colon","volume":"17","author":"Devasena","year":"2003","journal-title":"Phytother. Res."},{"key":"ref_25","unstructured":"NRC (2012). Nutrient Requirements of Swine, The National Academy Press. [11th ed.]."},{"key":"ref_26","unstructured":"Grela, E.R., and Skomia\u0142, J. (2015). Nutrient Requirements and Nutritional Value of Feeds for Swine, The Kielanowski Institute of Animal Physiology and Nutrition, PAS. [2nd ed.]. (In Polish)."},{"key":"ref_27","unstructured":"AOAC (2011). Official Methods of Analysis of AOAC International, AOAC International. [18th ed.]."},{"key":"ref_28","first-page":"1217","article-title":"Gravimetric determination of amylase-treated neutral detergent fibre in feeds with refluxing beakers and crucibles: Collaborative study","volume":"85","author":"Mertens","year":"2002","journal-title":"J. AOAC Int."},{"key":"ref_29","first-page":"313","article-title":"\u0170ber die Ver\u00f6nderung der Polyphenole in Verlauf des M\u00e4lzens und Maischens","volume":"25","author":"Jerumianis","year":"1972","journal-title":"Brauwissnschaft"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1104\/pp.55.1.1","article-title":"Composition and structure of protein bodies and spherosomes isolated from ungerminated seeds of Sorghum bicolor (Linn.)","volume":"55","author":"Adams","year":"1975","journal-title":"Moench. Plant Physiol."},{"key":"ref_31","first-page":"376","article-title":"Determination of trypsin inhibitor activity of soy products: Collaborative analysis of an improved procedure","volume":"51","author":"Kakade","year":"1974","journal-title":"Cereal Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"613","DOI":"10.22358\/jafs\/66219\/2011","article-title":"A dose-response effects of tannic acid and protein on growth performance, caecal fermentation, colon morphology, and \u03b2-glucuronidase activity of rats","volume":"20","author":"Barszcz","year":"2011","journal-title":"J. Anim. Feed Sci."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Taciak, M., Barszcz, M., Tu\u015bnio, A., and Pastuszewska, B. (2015). Interactive effects of indigestible carbohydrates, protein type, and protein level on biomarkers of large intestine health in rats. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0142176"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1080\/1745039X.2016.1184368","article-title":"The effects of inulin, dried Jerusalem artichoke tuber and a multispecies probiotic preparation on microbiota ecology and immune status of the large intestine in young pigs","volume":"70","author":"Barszcz","year":"2016","journal-title":"Arch. Anim. Nutr."},{"key":"ref_35","unstructured":"StatPoint Technologies, Inc. (2021, June 07). Statgraphics Centurion XVI Version 16.1.03. Available online: www.statgraphics.com."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0377-8401(01)00236-X","article-title":"Nutrient digestibility of chickpea (Cicer arietinum L.) seeds and effects on the small intestine of weaned piglets","volume":"91","author":"Salgado","year":"2001","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"So\u0144ta, M., Rekiel, A., Wi\u0119cek, J., Batorska, M., and Puppel, M. (2021). Alternative Protein Sources vs. GM Soybean Meal as Feedstuff for Pigs\u2014Meat Quality and Health-Promoting Indicators. Animals, 11.","DOI":"10.3390\/ani11010177"},{"key":"ref_38","first-page":"31","article-title":"Evaluation of faba beans (Vicia faba cv. Fiord) and sweet lupins (Lupinus albus cv. Kiev) as protein sources for growing pigs","volume":"25","author":"Brand","year":"1995","journal-title":"S. Afr. J. Anim. Sci."},{"key":"ref_39","first-page":"79","article-title":"Nutritional evaluation of dehulled faba bean (Vicia faba cv. Fiord) in feeds for weaner pigs","volume":"41","author":"Emiola","year":"2011","journal-title":"S. Afr. J. Anim. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"568","DOI":"10.22358\/jafs\/66685\/2008","article-title":"Effects of sodium butyrate on growth performance, intestinal microflora and morphology of weanling pigs","volume":"17","author":"Lu","year":"2008","journal-title":"J. Anim. Feed Sci."},{"key":"ref_41","first-page":"743","article-title":"Effect of faba bean tannins on the growth and histological structure of the intestinal tract of chicken and rats","volume":"35","author":"Ortiz","year":"1994","journal-title":"Brit. J. Nutr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1007\/BF02049730","article-title":"Transmural trophic effect of short chain fatty acid infusions on atrophic, defunctioned rat colon","volume":"38","author":"Mortensen","year":"1995","journal-title":"Dis. Colon Rectum."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1079\/BJN19940197","article-title":"Degradation of polyphenols (catechin and tannic acid) in the rat intestinal tract. Effect on colonic fermentation and faecal output","volume":"71","author":"Bravo","year":"1994","journal-title":"Br. J. Nutr."},{"key":"ref_44","unstructured":"Rowland, I.R. (1988). Bacterial metabolism of protein and endogenous nitrogen compounds. Role of the Gut Flora in Toxicity and Cancer, Academic Press."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"G1030","DOI":"10.1152\/ajpgi.00149.2010","article-title":"Colon luminal content and epithelial cell morphology are markedly modified in rats fed with a high-protein diet","volume":"299","author":"Andriamihaja","year":"2010","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_46","first-page":"51","article-title":"Protein Degradation in the Large Intestine: Relevance to Colorectal Cancer","volume":"1","author":"Hughes","year":"2000","journal-title":"Curr. Issues Intest. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"G105","DOI":"10.1152\/ajpgi.00437.2002","article-title":"Glucose alleviates ammonia-induced inhibition of short-chain fatty acid metabolism in rat colonic epithelial cells","volume":"285","author":"Cremin","year":"2003","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1007\/s00726-008-0218-3","article-title":"Colonic luminal ammonia and portal blood l-glutamine and l-arginine concentrations: A possible link between colon mucosa and liver ureagenesis","volume":"37","author":"Bernard","year":"2009","journal-title":"Amino Acids."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"G344","DOI":"10.1152\/ajpgi.00445.2003","article-title":"Adaptative increase of ornithine production and decrease of ammonia metabolism in rat colonocytes after hyperproteic diet ingestion","volume":"287","author":"Robert","year":"2004","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Oliphant, K., and Allen-Vercoe, E. (2019). Macronutrient metabolism by the human gut microbiome: Major fermentation by-products and their impact on host health. Microbiome, 7.","DOI":"10.1186\/s40168-019-0704-8"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/j.1365-2672.1991.tb02739.x","article-title":"The control and consequences of bacterial fermentation in the human colon","volume":"70","author":"Cummings","year":"1991","journal-title":"J. Appl. Bacteriol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.anaerobe.2006.04.001","article-title":"Profiling of composition and metabolic activities of the colonic microflora of growing pigs fed diets supplemented with prebiotic oligosaccharides","volume":"12","author":"Mountzouris","year":"2006","journal-title":"Anaerobe"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1079\/BJNBJN\/2002548","article-title":"Experimental evidences on the prebiotic fructans to reduce the risk of colon cancer","volume":"87","author":"Rowland","year":"2002","journal-title":"Brit. J. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.anifeedsci.2004.11.008","article-title":"Effects of copper bearing montmorillonite on the growth performance, intestinal microflora and morphology of weanling pigs","volume":"118","author":"Xia","year":"2005","journal-title":"Anim. Feed Sci. Technol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/6\/1703\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:11:44Z","timestamp":1760163104000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/6\/1703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,7]]},"references-count":54,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["ani11061703"],"URL":"https:\/\/doi.org\/10.3390\/ani11061703","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,7]]}}}