{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T14:22:09Z","timestamp":1791728529776,"version":"4.3.4"},"reference-count":101,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T00:00:00Z","timestamp":1623110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Here, we tested the changes occurring in several plasma analytes during different stages of the lactation cycle of high welfare raised multiparous Holstein cows, and provided reference intervals (RI) for plasma analytes concentrations. Eleven high-welfare farms (HWF) located in Northern Italy were selected and their herds used to recruit 361 clinically healthy cows undergoing the dry (from \u221230 to \u221210 days from real calving; DFC), the postpartum (from 3 to 7 DFC), the early lactation (from 28 to 45 DFC) and the late lactation phases (from 160 to 305 DFC). Cows affected by subclinical diseases (SCD) were retrospectively excluded, and a subset of 285 cows was selected. Data of plasma analytes underwent ANOVA testing using physiological phases as predictors. The individual effect of each phase was assessed using a pairwise t-test assuming p \u2264 0.05 as a significance limit. A bootstrap approach was used to define the reference interval (RI) for each blood analyte within physiological phases having a pairwise t-test p \u2264 0.05. The concentration of nonesterified fatty acids, albumin, cholesterol, retinol, paraoxonase and tocopherol changed throughout all the physiological phases, whereas the concentration of K, alkaline phosphatase and thiol groups remained stable. Triglycerides, Zn, and ferric ion reducing antioxidant power in the dry phase and BHB, Ca, myeloperoxidase, haptoglobin, reactive oxygen metabolites and advanced oxidation of protein product in postpartum differed compared with other physiological phases. During the dry phase, Packed cell volume, Cl, and urea concentrations were similar to during the postpartum phase. Similarly, Na, \u03b3-glutamyl transferase and \u03b2-carotene concentrations were similar to during the early lactation phase; fructosamine and bilirubin concentrations were similar to during the late lactation phase. During the postpartum phase, fructosamine and P concentrations were similar to during the early lactation phase, and the aspartate transaminase concentration was similar to during the late lactation phase. During the early lactation phase, Mg, creatinine, total protein, globulin and ceruloplasmin concentrations were similar to during the postpartum phase, while the urea concentration was similar to during the late lactation phase. All these plasma analytes differed among the other phases. This study identifies physiological trends affecting plasma analytes concentrations during the different stages of the lactation cycle and provides a guideline for the duration and magnitude of their changes when animals are healthy and raised in optimal welfare conditions.<\/jats:p>","DOI":"10.3390\/ani11061714","type":"journal-article","created":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T12:08:55Z","timestamp":1623154135000},"page":"1714","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Changes of Plasma Analytes Reflecting Metabolic Adaptation to the Different Stages of the Lactation Cycle in Healthy Multiparous Holstein Dairy Cows Raised in High-Welfare Conditions"],"prefix":"10.3390","volume":"11","author":[{"given":"Michele","family":"Premi","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, Food and Nutrition (DIANA), Facolt\u00e0 di Scienze Agrarie, Alimentari e Ambientali, Universit\u00e0 Cattolica del Sacro Cuore, 29122 Piacenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6554-0022","authenticated-orcid":false,"given":"Matteo","family":"Mezzetti","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Food and Nutrition (DIANA), Facolt\u00e0 di Scienze Agrarie, Alimentari e Ambientali, Universit\u00e0 Cattolica del Sacro Cuore, 29122 Piacenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9153-8540","authenticated-orcid":false,"given":"Giulia","family":"Ferronato","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Food and Nutrition (DIANA), Facolt\u00e0 di Scienze Agrarie, Alimentari e Ambientali, Universit\u00e0 Cattolica del Sacro Cuore, 29122 Piacenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7203-1549","authenticated-orcid":false,"given":"Mario","family":"Barbato","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Food and Nutrition (DIANA), Facolt\u00e0 di Scienze Agrarie, Alimentari e Ambientali, Universit\u00e0 Cattolica del Sacro Cuore, 29122 Piacenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiorenzo","family":"Piccioli Cappelli","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Food and Nutrition (DIANA), Facolt\u00e0 di Scienze Agrarie, Alimentari e Ambientali, Universit\u00e0 Cattolica del Sacro Cuore, 29122 Piacenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0617-6571","authenticated-orcid":false,"given":"Andrea","family":"Minuti","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Food and Nutrition (DIANA), Facolt\u00e0 di Scienze Agrarie, Alimentari e Ambientali, Universit\u00e0 Cattolica del Sacro Cuore, 29122 Piacenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1644-1911","authenticated-orcid":false,"given":"Erminio","family":"Trevisi","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Food and Nutrition (DIANA), Facolt\u00e0 di Scienze Agrarie, Alimentari e Ambientali, Universit\u00e0 Cattolica del Sacro Cuore, 29122 Piacenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/S0749-0720(15)30111-0","article-title":"Variability characteristics and test selection in herdlevel nutritional and metabolic profile testing","volume":"16","author":"Herdt","year":"2000","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_2","unstructured":"Piva, G., Bertoni, G., Masoero, F., Bani, P., and Calamari, L. (1999). Welfare, health and management of dairy cows. Recent Progress in Animal Production Science, FrancoAngeli."},{"key":"ref_3","first-page":"383","article-title":"Reference limits for biochemical and hematological analytes of dairy cows one week before and one week after parturition","volume":"50","author":"LeBlanc","year":"2009","journal-title":"Can. Vet. J. La Rev. Vet. Can."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"7886","DOI":"10.3168\/jds.2015-9345","article-title":"Short communication: Reference limits for blood analytes in Holstein late-pregnant heifers and dry cows: Effects of parity, days relative to calving, and season","volume":"98","author":"Brscic","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.rvsc.2017.06.012","article-title":"Reference intervals for hematological and biochemical parameters, acute phase proteins and markers of oxidation in Holstein dairy cows around 3 and 30 days after calving","volume":"114","author":"Moretti","year":"2017","journal-title":"Res. Vet. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.3168\/jds.2010-3687","article-title":"Short communication: Reference values for blood parameters in Holstein dairy cows: Effects of parity, stage of lactation, and season of production","volume":"94","author":"Cozzi","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.cvfa.2013.04.004","article-title":"Use of the liver activity index and other metabolic variables in the assessment of metabolic health in dairy herds","volume":"29","author":"Bertoni","year":"2013","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2259","DOI":"10.3168\/jds.S0022-0302(99)75474-3","article-title":"Biology of dairy cows during the transition period: The final frontier?","volume":"82","author":"Drackley","year":"1999","journal-title":"J. Dairy Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"323","DOI":"10.4081\/ijas.2005.323","article-title":"Physiological and pathological adaptations in dairy cows that may increase susceptibility to periparturient diseases and disorders","volume":"4","author":"Drackley","year":"2005","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"S155","DOI":"10.1093\/jas\/skaa134","article-title":"Interaction between inflammation and metabolism in periparturient dairy cows","volume":"98","author":"Mezzetti","year":"2020","journal-title":"J. Anim. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"165","DOI":"10.4141\/A04-043","article-title":"Strong relationships between mediators of the acute phase response and fatty liver in dairy cows","volume":"85","author":"Ametaj","year":"2005","journal-title":"Can. J. Anim. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5878","DOI":"10.3168\/jds.2010-3391","article-title":"Associations of prepartum plasma cortisol, haptoglobin, fecal cortisol metabolites, and nonesterified fatty acids with postpartum health status in Holstein dairy cows","volume":"94","author":"Huzzey","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"301","DOI":"10.4081\/ijas.2009.s1.301","article-title":"Model to evaluate welfare in dairy cow farms","volume":"8","author":"Calamari","year":"2009","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_14","unstructured":"Welfare Quality\u00ae Consortium (2009). Welfare Quality\u00ae Assessment Protocol for Cattle, Welfare Quality."},{"key":"ref_15","unstructured":"Jordan, K., Cook, N., Darr, D., DeCoite, C., Doak, R., Endres, M., Humphrey, P., Keyserlingk, N.V., Maddox, S., and Mahoney, J. (2016). Farmers Assuring Responsible Management: Animal Care Reference Manual, FARM."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1017\/S0022029920000461","article-title":"Systems for evaluation of welfare on dairy farms","volume":"87","author":"Krueger","year":"2020","journal-title":"J. Dairy Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0749-0720(15)30103-1","article-title":"Subclinical ketosis in lactating dairy cattle","volume":"16","author":"Duffield","year":"2000","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.tvjl.2007.12.020","article-title":"The monitoring, prevention, and treatment of milk fever and subclinical hypocalcemia in dairy cows","volume":"176","author":"Goff","year":"2008","journal-title":"Vet. J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"9241","DOI":"10.3168\/jds.2019-16497","article-title":"The role of altered immune function during the dry period in promoting the development of subclinical ketosis in early lactation","volume":"102","author":"Mezzetti","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1017\/S175173111200170X","article-title":"Nutrition, immune function and health of dairy cattle","volume":"7","author":"Ingvartsen","year":"2013","journal-title":"Animal"},{"key":"ref_21","unstructured":"Calamari, L., Bionaz, M., Trevisi, E., and Bertoni, G. (2004, January 2\u20134). Preliminary study to validate a model of animal welfare assessment in dairy farms. Proceedings of the Fifth Congress of the European Society for Agricultural and Food Ethics (EURSAFE), Leuven, Belgium."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"18","DOI":"10.21423\/bovine-vol42no1p18-26","article-title":"Body temperature and white blood cell count in postpartum dairy cows","volume":"42","author":"Wagner","year":"2008","journal-title":"Bov. Pract."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.cvfa.2005.02.007","article-title":"Management of periparturient disorders in dairy cattle","volume":"21","author":"Smith","year":"2005","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"524","DOI":"10.3168\/jds.S0022-0302(04)73192-6","article-title":"Using activity and milk yield as predictors of fresh cow disorders","volume":"87","author":"Edwards","year":"2004","journal-title":"J. Dairy Sci."},{"key":"ref_25","unstructured":"(1986). Agricultural Development and Advisory Service Condition scoring of dairy cows, Proceedings of the Publication 612."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5769","DOI":"10.3168\/jds.2009-2431","article-title":"Invited review: Body condition score and its association with dairy cow productivity, health, and welfare","volume":"92","author":"Roche","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Calamari, L., Ferrari, A., Minuti, A., and Trevisi, E. (2016). Assessment of the main plasma parameters included in a metabolic profile of dairy cow based on Fourier Transform mid-infrared spectroscopy: Preliminary results. BMC Vet. Res., 12.","DOI":"10.1186\/s12917-015-0621-4"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"7717","DOI":"10.3168\/jds.2015-9359","article-title":"Maternal consumption of organic trace minerals (4-Plex) alters calf systemic and neutrophil mRNA and microRNA indicators of inflammation and oxidative stress","volume":"98","author":"Jacometo","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1740","DOI":"10.3168\/jds.2006-445","article-title":"Plasma paraoxonase, health, inflammatory conditions, and liver function in transition dairy cows","volume":"90","author":"Bionaz","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3966","DOI":"10.2527\/jas.2014-7594","article-title":"Experimental acute rumen acidosis in sheep: Consequences on clinical, rumen, and gastrointestinal permeability conditions and blood chemistry","volume":"92","author":"Minuti","year":"2014","journal-title":"J. Anim. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1111\/1523-1747.ep12506462","article-title":"Measurement of cutaneous inflammation: Estimation of neutrophil content with an enzyme marker","volume":"78","author":"Bradley","year":"1982","journal-title":"J. Investig. Dermatol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3300","DOI":"10.3168\/jds.2008-0995","article-title":"Effects of inflammatory conditions on liver activity in puerperium period and consequences for performance in dairy cows","volume":"91","author":"Bertoni","year":"2008","journal-title":"J. Dairy Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.livsci.2018.08.003","article-title":"Plasma fructosamine during the transition period and its relationship with energy metabolism and inflammation biomarkers in dairy cows","volume":"216","author":"Trevisi","year":"2018","journal-title":"Livest. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/j.cca.2012.01.038","article-title":"Improved detection of advanced oxidation protein products in plasma","volume":"413","author":"Hanasand","year":"2012","journal-title":"Clin. Chim. Acta"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.vetimm.2015.04.003","article-title":"Assessment of immune response in periparturient dairy cows using ex vivo whole blood stimulation assay with lipopolysaccharides and carrageenan skin test","volume":"165","author":"Jahan","year":"2015","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_36","unstructured":"The R Core Team (2012). R: A Language and Environment for Statistical Computing, R Project for Statistical Computing."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1111\/vcp.12006","article-title":"ASVCP reference interval guidelines: Determination of de novo reference intervals in veterinary species and other related topics","volume":"41","author":"Friedrichs","year":"2012","journal-title":"Vet. Clin. Pathol."},{"key":"ref_38","first-page":"503","article-title":"Blood concentrations of beta hydroxybutyrate in clinically normal Holstein-Friesian herds and in those with a high prevalence of clinical ketosis","volume":"42","author":"Herdt","year":"1981","journal-title":"Am. J. Vet. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.tvjl.2010.03.025","article-title":"Prevalence of subclinical hypocalcemia in dairy herds","volume":"188","author":"Reinhardt","year":"2011","journal-title":"Vet. J."},{"key":"ref_40","unstructured":"Signorell, A., Aho, K., Alfons, A., Anderegg, N., Aragon, T., Arachchige, C., Arppe, A., Baddeley, A., Barton, K., and Bolker, B. (2021, June 07). DescTools: Tools for Descriptive Statistics. Available online: https:\/\/cran.r-project.org\/package=DescTools."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.smallrumres.2009.03.004","article-title":"A bootstrap approach to estimate reference intervals of biochemical variables in sheep using reduced sample sizes","volume":"83","author":"Dimauro","year":"2009","journal-title":"Small Rumin. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2236","DOI":"10.3168\/jds.S0022-0302(84)81571-4","article-title":"Blood constituents in lactating holstein cows influenced by hematocrit, sampling site, and diet protein and calcium","volume":"67","author":"Wohlt","year":"1984","journal-title":"J. Dairy Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.3168\/jds.S0022-0302(91)78353-7","article-title":"Water, sodium, potassium, and chlorine metabolism of dairy cows at the onset of lactation in hot weather","volume":"74","author":"Shalit","year":"1991","journal-title":"J. Dairy Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1136\/bmj.287.6396.863","article-title":"Clinical usefulness of estimation of serum fructosamine concentration as a screening test for diabetes mellitus","volume":"287","author":"Baker","year":"1983","journal-title":"BMJ"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1093\/clinchem\/33.12.2153","article-title":"Fructosamine: Structure, analysis, and clinical usefulness","volume":"33","author":"Armbruster","year":"1987","journal-title":"Clin. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0749-0720(15)30102-X","article-title":"Ruminant adaptation to negative energy balance. Influences on the etiology of ketosis and fatty liver","volume":"16","author":"Herdt","year":"2000","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_47","first-page":"6","article-title":"Peripartum liver triglyceride and plasma metabolites in dairy cows","volume":"77","author":"Bertics","year":"1994","journal-title":"J. Dairy Sci."},{"key":"ref_48","first-page":"13","article-title":"Nutrition, negative energy balance and fertility in the postpartum dairy cow","volume":"13","author":"Butler","year":"2005","journal-title":"Cattle Pract."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1023\/A:1026336505343","article-title":"Adaptations of glucose metabolism during pregnancy and lactation","volume":"2","author":"Bell","year":"1997","journal-title":"J. Mammary Gland Biol. Neoplasia"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2360","DOI":"10.3168\/jds.2016-12001","article-title":"Glucose requirements of an activated immune system in lactating Holstein cows","volume":"100","author":"Kvidera","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/s11250-016-1211-6","article-title":"Significance of insulin resistance and oxidative stress in dairy cattle with subclinical ketosis during the transition period","volume":"49","author":"Youssef","year":"2017","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_52","first-page":"13","article-title":"Nutrition during the dry period and hepatic lipid metabolism of periparturient dairy cows","volume":"13","author":"Grum","year":"1996","journal-title":"J. Dairy Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3314","DOI":"10.3168\/jds.S0022-0302(02)74420-2","article-title":"Estimation of energy balance at the individual and herd level using blood and milk traits in high-yielding dairy cows","volume":"85","author":"Reist","year":"2002","journal-title":"J. Dairy Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.3168\/jds.2010-3303","article-title":"Prepartum dietary energy intake affects metabolism and health during the periparturient period in primiparous and multiparous Holstein cows","volume":"94","author":"Janovick","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.3168\/jds.S0022-0302(92)77951-X","article-title":"Effect of prepartum dry matter intake on liver triglyceride concentration and early lactation","volume":"75","author":"Bertics","year":"1992","journal-title":"J. Dairy Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2028","DOI":"10.3168\/jds.S0022-0302(89)79326-7","article-title":"Seasonal effects of prepartum and postpartum fat and niacin feeding on lactation performance and lipid metabolism","volume":"72","author":"Skaar","year":"1989","journal-title":"J. Dairy Sci."},{"key":"ref_57","unstructured":"Saunders, W.B. (2002). Gastrointestinal physiology and metabolism. Textbook of Veterinary Physiology, Elsevier."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"545","DOI":"10.2527\/2003.812545x","article-title":"Nitrogen metabolism and recycling in Holstein heifers","volume":"81","author":"Marini","year":"2003","journal-title":"J. Anim. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.3168\/jds.S0022-0302(05)72884-8","article-title":"Effects of two different feeding strategies during dry-off on metabolism in high-yielding dairy cows","volume":"88","author":"Odensten","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"7437","DOI":"10.3168\/jds.2013-7679","article-title":"Biomarkers of inflammation, metabolism, and oxidative stress in blood, liver, and milk reveal a better immunometabolic status in peripartal cows supplemented with Smartamine M or MetaSmart","volume":"97","author":"Osorio","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"6423","DOI":"10.3168\/jds.2013-6801","article-title":"Effects of body condition score at calving on indicators of fat and protein mobilization of periparturient Holstein-Friesian cows","volume":"96","author":"Pires","year":"2013","journal-title":"J. Dairy Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"389","DOI":"10.15232\/aas.2019-01929","article-title":"Invited review: Quantifying protein mobilization in dairy cows during the transition period","volume":"36","author":"McCabe","year":"2020","journal-title":"Appl. Anim. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"S64","DOI":"10.1681\/ASN.2005121345","article-title":"Inflammation as a mediator of the link between mild to moderate renal insufficiency and endothelial dysfunction in essential hypertension","volume":"4","author":"Zoccali","year":"2006","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1111\/j.1471-0528.1974.tb00522.x","article-title":"Glomerular filtration during and after pregnancy","volume":"81","author":"Davison","year":"1974","journal-title":"BJOG An. Int. J. Obstet. Gynaecol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Thompson, M., Ray, U., Yu, R., Hudspeth, A., Smillie, M., Jordan, N., and Bartle, J. (2016). Kidney function as a determinant of HDL and triglyceride concentrations in the australian population. J. Clin. Med., 5.","DOI":"10.3390\/jcm5030035"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1023\/A:1026384421273","article-title":"Calcium and vitamin D metabolism during lactation","volume":"2","author":"Horst","year":"1997","journal-title":"J. Mammary Gland Biol. Neoplasia"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1152\/ajplegacy.1970.219.5.1166","article-title":"Calcium kinetics in cows during late pregnancy, parturition, and early lactation","volume":"219","author":"Ramberg","year":"1970","journal-title":"Am. J. Physiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1439-0442.2004.00679.x","article-title":"Studies of the transition cow under a pasture-based milk production system: Metabolic profiles","volume":"52","author":"Cavestany","year":"2005","journal-title":"J. Vet. Med. Ser. A Physiol. Pathol. Clin. Med."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"3582","DOI":"10.3168\/jds.S0022-0302(05)73043-5","article-title":"Periparturient responses of multiparous holstein cows fed different dietary phosphorus concentrations prepartum","volume":"88","author":"Peterson","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.3168\/jds.2013-7467","article-title":"Diet-induced pseudohypoparathyroidism: A hypocalcemia and milk fever risk factor","volume":"97","author":"Goff","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/S0749-0720(15)30167-5","article-title":"Treatment of calcium, phosphorus, and magnesium balance disorders","volume":"15","author":"Goff","year":"1999","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"3635","DOI":"10.3168\/jds.2013-7709","article-title":"Rumination time around calving: An early signal to detect cows at greater risk of disease","volume":"97","author":"Calamari","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1172\/JCI102725","article-title":"Hexamethonium\u2014Its effect on glomerular filtration rate, maximal tubular function, and renal excretion of electrolytes","volume":"32","author":"Moyer","year":"1953","journal-title":"J. Clin. Investig."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"441","DOI":"10.2527\/jas1965.242441x","article-title":"Renal function of cattle under various water and salt loads","volume":"24","author":"Weeth","year":"1965","journal-title":"J. Anim. Sci."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s11906-009-0007-8","article-title":"Stress-induced sodium retention and hypertension: A review and hypothesis","volume":"11","author":"Harshfield","year":"2009","journal-title":"Curr. Hypertens. Rep."},{"key":"ref_76","unstructured":"Committee on Mineral Nutrition (1973). Tracing and Treating Mineral. Disorders in Dairy Cattle, Centre for Agricultural Publishing and Documentation."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1080\/00480169.1976.34283","article-title":"Plasma zinc status of dairy cattle in the periparturient period","volume":"24","author":"Pryor","year":"1976","journal-title":"N. Z. Vet. J."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1111\/j.1751-0813.1977.tb07935.x","article-title":"The plasma zinc concentration of nonpregnant, pregnant and parturient hereford cattle","volume":"53","author":"Dufty","year":"1977","journal-title":"Aust. Vet. J."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"8224","DOI":"10.3168\/jds.2017-14254","article-title":"Prepartal standing behavior as a parameter for early detection of postpartal subclinical ketosis associated with inflammation and liver function biomarkers in peripartal dairy cows","volume":"101","author":"Haerr","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"918","DOI":"10.3168\/jds.2012-5676","article-title":"Liver lipid content and inflammometabolic indices in peripartal dairy cows are altered in response to prepartal energy intake and postpartal intramammary inflammatory challenge","volume":"96","author":"Graugnard","year":"2013","journal-title":"J. Dairy Sci."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1080\/03079458808436507","article-title":"Enzyme activities in tissues and elimination half-lives of homologous muscle and liver enzymes in the racing pigeon (Columba Livia domestica)","volume":"17","author":"Lumeij","year":"1988","journal-title":"Avian Pathol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.cvfa.2013.03.002","article-title":"Significance of metabolic stress, lipid mobilization, and inflammation on transition cow disorders","volume":"29","author":"Sordillo","year":"2013","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.cca.2008.06.016","article-title":"How reliable an indicator of inflammation is myeloperoxidase activity?","volume":"396","author":"Faith","year":"2008","journal-title":"Clin. Chim. Acta"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"10271","DOI":"10.1016\/S0021-9258(18)34015-8","article-title":"The acute phase response of plasma protein synthesis during experimental inflammation","volume":"257","author":"Schreiber","year":"1982","journal-title":"J. Biol. Chem."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1079\/PNS19890050","article-title":"Clinical and nutritional aspects of changes in acute-phase proteins during inflammation","volume":"48","author":"Fleck","year":"1989","journal-title":"Proc. Nutr. Soc."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"4207","DOI":"10.1016\/j.jprot.2012.04.004","article-title":"Acute phase proteins in ruminants","volume":"75","author":"Ceciliani","year":"2012","journal-title":"J. Proteomics"},{"key":"ref_87","first-page":"513","article-title":"Haptoglobin: A review","volume":"6","author":"Giblett","year":"1961","journal-title":"Int. J. Transfus. Med."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1111\/j.1939-165X.2006.tb00112.x","article-title":"Relationship between haptoglobin, serum amyloid A, and clinical status in a survey of dairy herds during a 6-month period","volume":"35","author":"Humblet","year":"2006","journal-title":"Vet. Clin. Pathol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"265","DOI":"10.4081\/ijas.2009.s1.265","article-title":"Some physiological and biochemical methods for acute and chronic stress evaluationin dairy cows","volume":"8","author":"Trevisi","year":"2009","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0891-5849(94)00102-P","article-title":"A fluorescence-based method for measuring total plasma antioxidant capability","volume":"18","author":"Ghiselli","year":"1995","journal-title":"Free Radic. Biol. Med."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The ferric reducing ability of plasma (FRAP) as a measure of \u201cantioxidant power\u201d: The FRAP assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.vetimm.2008.10.305","article-title":"Impact of oxidative stress on the health and immune function of dairy cattle","volume":"128","author":"Sordillo","year":"2009","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3389\/fvets.2015.00048","article-title":"Oxidant\/antioxidant balance in animal nutrition and health: The role of protein oxidation","volume":"2","author":"Celi","year":"2015","journal-title":"Front. Vet. Sci."},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Mandelker, L., and Vajdovich, P. (2011). Oxidative stress in ruminants. Studies on Veterinary Medicine. Oxidative Stress in Applied Basic Research and Clinical Practice, Humana Press.","DOI":"10.1007\/978-1-61779-071-3"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1017\/S1466252309990016","article-title":"Metabolic factors affecting the inflammatory response of periparturient dairy cows","volume":"10","author":"Sordillo","year":"2009","journal-title":"Anim. Health Res. Rev."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.3945\/ajcn.110.008490","article-title":"The relation between acute changes in the systemic inflammatory response and plasma 25-hydroxyvitamin D concentrations after elective knee arthroplasty","volume":"93","author":"Reid","year":"2011","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_97","unstructured":"Harinder, P.S. (2013). Use of nutraceuticals for improving animal health during the transition period of dairy cows. Enhancing Animal Welfare and Farmer Income through Strategic Animal Feeding. Some Case Studies, Food and Agriculture Organization of the United Nations (FAO)."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.cvfa.2007.07.002","article-title":"Evaluation of the ruminant complete blood cell count","volume":"23","author":"Jones","year":"2007","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1136\/vr.128.7.147","article-title":"Bovine haptoglobin response in clinically defined field conditions","volume":"128","author":"Skinner","year":"1991","journal-title":"Vet. Rec."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1093\/clinchem\/16.11.903","article-title":"Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity","volume":"16","author":"Sunderman","year":"1970","journal-title":"Clin. Chem."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1093\/clinchem\/48.2.261","article-title":"Serum paraoxonase activity: A new additional test for the improved evaluation of chronic liver damage","volume":"48","author":"Camps","year":"2002","journal-title":"Clin. Chem."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/6\/1714\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:12:05Z","timestamp":1760163125000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/6\/1714"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,8]]},"references-count":101,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["ani11061714"],"URL":"https:\/\/doi.org\/10.3390\/ani11061714","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,8]]}}}