{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:08:12Z","timestamp":1791346092860,"version":"4.3.1"},"reference-count":56,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T00:00:00Z","timestamp":1625616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MINISTERIO DE ECONOMIA Y COMPETITIVIDAD","award":["AGL2014-54683-R"],"award-info":[{"award-number":["AGL2014-54683-R"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>On intensive fish farms, 10% of the population dies exclusively from pathogens, and Photobacterium damselae subsp. Piscicida (Ph. damselae subsp. Piscicida), the bacteria causing pasteurellosis in marine aquaculture, is one of the major pathogens involved. The objective of this study was to obtain new probiotic strains against pasteurellosis in order to limit the use of chemotherapy, avoiding the environmental repercussions generated by the abusive use of these products. In this study, 122 strains were isolated from the gills and intestines of different marine fish species and were later evaluated in vitro to demonstrate the production of antagonistic effects, the production of antibacterial substances, adhesion and growth to mucus, resistance to bile and resistance to pH gradients, as well as its harmlessness and the dynamic of expression of immune-related genes by real-time PCR after administration of the potential probiotic in the fish diet. Only 1\/122 strains showed excellent results to be considered as a potential probiotic strain and continue its characterization against Ph. damselae subsp. piscicida to determine its protective effect and elucidating in future studies its use as a possible probiotic strain for marine aquaculture.<\/jats:p>","DOI":"10.3390\/ani11072029","type":"journal-article","created":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T12:31:25Z","timestamp":1625661085000},"page":"2029","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Screening of New Potential Probiotics Strains against Photobacterium damselae Subsp. piscicida for Marine Aquaculture"],"prefix":"10.3390","volume":"11","author":[{"given":"Ana","family":"Guti\u00e9rrez Falc\u00f3n","sequence":"first","affiliation":[{"name":"University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6678-5029","authenticated-orcid":false,"given":"Daniel","family":"Padilla","sequence":"additional","affiliation":[{"name":"University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6526-0354","authenticated-orcid":false,"given":"Fernando","family":"Real","sequence":"additional","affiliation":[{"name":"University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Jos\u00e9","family":"Ramos Sosa","sequence":"additional","affiliation":[{"name":"University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bego\u00f1a","family":"Acosta-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"S\u00e1nchez Henao","sequence":"additional","affiliation":[{"name":"University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5027-3072","authenticated-orcid":false,"given":"Natalia","family":"Garc\u00eda-\u00c1lvarez","sequence":"additional","affiliation":[{"name":"University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inmaculada","family":"Rosario Medina","sequence":"additional","affiliation":[{"name":"University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Freddy","family":"Silva Sergent","sequence":"additional","affiliation":[{"name":"University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0201-8338","authenticated-orcid":false,"given":"Soraya","family":"D\u00e9niz","sequence":"additional","affiliation":[{"name":"University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3280-9838","authenticated-orcid":false,"given":"Jos\u00e9 Lu\u00eds","family":"Mart\u00edn-Barrasa","sequence":"additional","affiliation":[{"name":"University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35413 Arucas, Spain"},{"name":"Research Unit, Hospital Universitario de Gran Canaria, Dr Negr\u00edn, 35019 Las Palmas de Gran Canaria, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/0959-8030(93)90036-B","article-title":"Viral vaccines for aquaculture","volume":"3","author":"Leong","year":"1993","journal-title":"Ann. 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