{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:11:16Z","timestamp":1760235076290,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,17]],"date-time":"2021-07-17T00:00:00Z","timestamp":1626480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>It is difficult to objectively assess the chronic effects of housing systems on livestock and particularly on laying hens. However, this seems to be important in the context of animal welfare. Therefore, we conducted the present study in order to compare the effect of two different housing conditions, single cage (SC) and floor pen (FP), on the morphology of the adrenal gland. A higher amount of interrenal cells, which secrete stress hormones, can lead to a difference in the relation of adrenal and interrenal cells, which could be interpreted as an indication of chronic stress. For this purpose, adrenal glands were extracted, prepared, stained and examined by microscopy, and total area of the cut, total area of interrenal cells and total area of adrenal cells were measured. As a result, all laying hens had a higher percentage of interrenal cells than adrenal cells (FP: interrenal cells\/adrenal cells = 78.37%\/21.63%; SC: 80.00%\/20.00%). The median of adrenal\u2013interrenal ratio did not differ significantly (FP = 0.2503, SC = 0.2499), while the variation of the ratio between laying hens in FP and SC showed a slight tendency of a higher ratio in adrenal glands of FP (p &lt; 0.0870). Body weight and adrenal\u2013interrenal ratio were significantly negatively correlated in laying hens in FP (rS = \u22120.943, p &lt; 0.0048) but not in SC (rS = \u22120.162, p = 0.7283). There was no significant correlation between body weight and total cell area for interrenal cells or adrenal cells. Body weight was significantly lower for laying hens kept in SC than for laying hens kept in FP (p &lt; 0.0001). Due to the present results, it can be concluded that keeping laying hens in single cages can have a negative effect on body weight.<\/jats:p>","DOI":"10.3390\/ani11072124","type":"journal-article","created":{"date-parts":[[2021,7,18]],"date-time":"2021-07-18T21:16:48Z","timestamp":1626643008000},"page":"2124","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigation of the Morphology of Adrenal Glands in Hens Kept in Two Different Housing Systems\u2014A Pilot Study"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0432-5880","authenticated-orcid":false,"given":"Franziska","family":"Ke\u00dfler","sequence":"first","affiliation":[{"name":"Institute of Animal Welfare and Animal Husbandry, Friedrich-Loeffler-Institut, 29223 Celle, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelika","family":"Gr\u00fcmpel-Schl\u00fcter","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition, Friedrich-Loeffler-Institut, 38116 Braunschweig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Looft","sequence":"additional","affiliation":[{"name":"Department of Animal Breeding and Husbandry, University of Applied Science Neubrandenburg, 17033 Neubrandenburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Petow","sequence":"additional","affiliation":[{"name":"Institute of Animal Welfare and Animal Husbandry, Friedrich-Loeffler-Institut, 29223 Celle, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,17]]},"reference":[{"key":"ref_1","unstructured":"Windhorst, H.-W. 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