{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:30:40Z","timestamp":1792042240024,"version":"4.4.0"},"reference-count":35,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,7,31]],"date-time":"2021-07-31T00:00:00Z","timestamp":1627689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003668","name":"Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries","doi-asserted-by":"publisher","award":["321037-05-1-HD030"],"award-info":[{"award-number":["321037-05-1-HD030"]}],"id":[{"id":"10.13039\/501100003668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Dietary yeast cell wall products (YCW) are recognized as a feed additive due to multifunctional benefits by the biological response modulators. Thus, this study was conducted to verify a potential advantage of YCW for improving growth performance, nutrient digestibility, immune responses, and intestinal health and microbiota of weaned pigs. A total of 112 weaned pigs (7.99 \u00b1 1.10 kg of body weight; 28 days old) were arbitrarily allocated to two experimental treatments with eight pigs (four barrows and four gilts) per pen and seven replicate pens per treatment in a completely randomized block design (block = BW and sex): (1) a basal diet based on corn and soybean meal (CON) and (2) CON + 0.05% YCW. The experimental period was for 4 weeks. There were no differences in final body weight, average daily feed intake, and gain-to-feed ratio between dietary treatments. In contrast, pigs fed YCW had higher average daily gain (p = 0.088) and apparent ileal digestibility of DM (p &lt; 0.05) and energy (p = 0.052) and lower diarrhea frequency (p = 0.083) than those fed control diet (CON). Pigs fed YCW also had a higher (p &lt; 0.05) ratio between villus height and crypt depth, villus width and area, and goblet cell counts in the duodenum and\/or jejunum than those fed CON. Dietary YCW decreased (p &lt; 0.05) serum TNF-\u03b1 and IL\u20131\u03b2 of weaned pigs on day 7 and 14, respectively, compared with CON. Furthermore, pigs fed YCW had higher (p &lt; 0.05) ileal gene expression of claudin family, occludin, MUC1, INF-\u03b3, and IL-6 and lower (p &lt; 0.05) that of TNF-\u03b1 than those fed CON. Lastly, there were no differences in the relative abundance of bacteria at the phylum level between CON and YCW. However, dietary YCW increased (p &lt; 0.05) the relative abundance of genera Prevotella and Roseburia compared with CON. This study provided that dietary YCW improved growth rate, nutritional digestibility, and intestinal health and modified immune responses and intestinal microbiota of weaned pigs.<\/jats:p>","DOI":"10.3390\/ani11082269","type":"journal-article","created":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T21:51:07Z","timestamp":1627854667000},"page":"2269","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Dietary Yeast Cell Wall Improves Growth Performance and Prevents of Diarrhea of Weaned Pigs by Enhancing Gut Health and Anti-Inflammatory Immune Responses"],"prefix":"10.3390","volume":"11","author":[{"given":"Jeong Jae","family":"Lee","sequence":"first","affiliation":[{"name":"Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyunjin","family":"Kyoung","sequence":"additional","affiliation":[{"name":"Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Ho","family":"Cho","sequence":"additional","affiliation":[{"name":"Division of Food and Animal Science, Chungbuk National University, Cheongju 28644, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7217-972X","authenticated-orcid":false,"given":"Jeehwan","family":"Choe","sequence":"additional","affiliation":[{"name":"Department of Beef Science, Korea National College of Agriculture and Fisheries, Jeonju 54874, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6769-0657","authenticated-orcid":false,"given":"Younghoon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joowon","family":"Kang","sequence":"additional","affiliation":[{"name":"Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanbae","family":"Lee","sequence":"additional","affiliation":[{"name":"Pathway Intermediates, Seoul 06253, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeun Bum","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Resources Science, Dankook University, Cheonan 31116, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4515-5212","authenticated-orcid":false,"given":"Minho","family":"Song","sequence":"additional","affiliation":[{"name":"Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0167-5877(97)00057-3","article-title":"Measurement of digestive disorders in the piglet at weaning and related risk factors","volume":"35","author":"Madec","year":"1998","journal-title":"Prev. Vet. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1051\/animres:2004018","article-title":"Gut function and dysfunction in young pigs: Physiology","volume":"53","author":"Lalles","year":"2004","journal-title":"Anim. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1152\/ajpgi.00081.2009","article-title":"Early weaning stress impairs development of mucosal barrier function in the porcine intestine","volume":"298","author":"Smith","year":"2010","journal-title":"Am. J. Physiol.-Gastroint. Liver Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"767","DOI":"10.2527\/jas.2013-6793","article-title":"The effects of live yeast Saccharomyces cerevisiae on postweaning diarrhea, immune response, and growth performance in weaned piglets","volume":"92","author":"Trckova","year":"2014","journal-title":"J. Anim. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1017\/S0007114517003051","article-title":"Effects of dietary live yeast supplementation on growth performance, diarrhoea severity, intestinal permeability and immunological parameters of weaned piglets challenged with enterotoxigenic Escherichia coli K88","volume":"118","author":"Che","year":"2017","journal-title":"Br. J. Nutr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1007\/s002530051278","article-title":"Composition of the cell walls of several yeast species","volume":"50","author":"Nguyen","year":"1998","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2614","DOI":"10.2527\/jas.2008-1512","article-title":"Effects of yeast culture supplementation on growth performance, intestinal health, and immune response of nursery pigs","volume":"87","author":"Shen","year":"2009","journal-title":"J. Anim. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"fnv122","DOI":"10.1093\/femsle\/fnv122","article-title":"Prebiotics and gut microbiota in chickens","volume":"362","author":"Pourabedin","year":"2015","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.anifeedsci.2005.12.015","article-title":"Live yeast dietary supplementation acts upon intestinal morpho-functional aspects and growth in weanling piglets","volume":"129","author":"Bontempo","year":"2006","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.livsci.2007.01.134","article-title":"Role of yeast cell wall polysaccharides in pig nutrition and health protection","volume":"109","author":"Kogan","year":"2007","journal-title":"Livest. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.anifeedsci.2014.04.020","article-title":"Effects of inclusion of hydrolyzed yeast on the immune response and performance of piglets after weaning","volume":"195","author":"Molist","year":"2014","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"21","DOI":"10.5187\/jast.2020.62.1.21","article-title":"Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs","volume":"62","author":"Park","year":"2020","journal-title":"J. Anim. Sci. Technol."},{"key":"ref_13","unstructured":"NRC (2012). Nutrient Requirements of Swine, The National Academies Press. [11th ed.]."},{"key":"ref_14","unstructured":"AOAC (2019). International Official Methods of Analysis, AOAC International. [21st ed.]."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1017\/S002185960001546X","article-title":"The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry","volume":"59","author":"Williams","year":"1962","journal-title":"J. Agric. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"172","DOI":"10.2527\/jas.2005-742","article-title":"Invited review: Amino acid bioavailability and digestibility in pig feed ingredients: Terminology and application","volume":"85","author":"Stein","year":"2007","journal-title":"J. Anim. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"298","DOI":"10.2527\/jas.2014-7259","article-title":"Spray-dried plasma attenuates inflammation and improves pregnancy rate of mated female mice","volume":"93","author":"Song","year":"2015","journal-title":"J. Anim. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Kuijk, E.W., du Puy, L., van Tol, H.T.A., Haagsman, H.P., Colenbrander, B., and Roelen, B.A.J. (2007). Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos. BMC Dev. Biol., 7.","DOI":"10.1186\/1471-213X-7-58"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5112","DOI":"10.1128\/AEM.01043-13","article-title":"Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform","volume":"79","author":"Kozich","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"7537","DOI":"10.1128\/AEM.01541-09","article-title":"Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities","volume":"75","author":"Schloss","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat. Methods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"174","DOI":"10.5187\/jast.2020.62.2.174","article-title":"Effects of dietary protease on immune responses of weaned pigs","volume":"62","author":"Lee","year":"2020","journal-title":"J. Anim. Sci. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1111\/asj.12195","article-title":"Effects of Saccharomyces cerevisiae cell wall extract and poplar propolis ethanol extract supplementation on growth performance, digestibility, blood profile, fecal microbiota and fecal noxious gas emissions in growing pigs","volume":"85","author":"Li","year":"2014","journal-title":"Anim. Sci. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2534","DOI":"10.1093\/jas\/skz150","article-title":"Effect of live yeast supplementation to gestating sows and nursery piglets on postweaning growth performance and nutrient digestibility","volume":"97","author":"Lu","year":"2019","journal-title":"J. Anim. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.livsci.2014.10.012","article-title":"Dietary supplementation with yeast product improves intestinal function, and serum and ileal amino acid contents in weaned piglets","volume":"171","author":"Xiong","year":"2015","journal-title":"Livest. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.1017\/S1751731117001276","article-title":"Dietary supplementation with a nucleotide-rich yeast extract modulates gut immune response and microflora in weaned pigs in response to a sanitary challenge","volume":"11","author":"Waititu","year":"2017","journal-title":"Animal"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1152\/ajpgi.00055.2011","article-title":"Occludin regulates macromolecule flux across the intestinal epithelial tight junction barrier","volume":"300","author":"Khatib","year":"2011","journal-title":"Am. J. Physiol.-Gastroint. Liver Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1097\/MIB.0000000000000280","article-title":"Saccharomyces cerevisiae CNCM I-3856 prevents colitis Induced by AIEC bacteria in the transgenic mouse model mimicking crohn\u2019s disease","volume":"21","author":"Sivignon","year":"2015","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2359","DOI":"10.1039\/C8FO02327A","article-title":"Effects of dietary supplementation with yeast glycoprotein on growth performance, intestinal mucosal morphology, immune response and colonic microbiota in weaned piglets","volume":"10","author":"Qin","year":"2019","journal-title":"Food Funct."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.3389\/fmicb.2018.02011","article-title":"Yeast probiotics shape the gut microbiome and improve the health of early-weaned piglets","volume":"9","author":"Xu","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1073\/pnas.1120238109","article-title":"In-feed antibiotic effects on the swine intestinal microbiome","volume":"109","author":"Looft","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"157","DOI":"10.2217\/fmb-2016-0130","article-title":"Roseburia spp.: A marker of health?","volume":"12","author":"Smida","year":"2017","journal-title":"Future Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"359","DOI":"10.5187\/jast.2019.61.6.359","article-title":"Effects of dietary carbohydrases on productive performance and immune responses of lactating sows and their piglets","volume":"61","author":"Lee","year":"2019","journal-title":"J. Anim. Sci. Technol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"642","DOI":"10.4049\/jimmunol.177.1.642","article-title":"A Regulatory Effect of the Balance between TNF-\u03b1 and IL-6 in the Granulomatous and Inflammatory Response to Rhodococcus aurantiacus Infection in Mice","volume":"177","author":"Yimin","year":"2006","journal-title":"J. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1017\/S1751731116001166","article-title":"Bacteriophage cocktail and multi-strain probiotics in the feed for weanling pigs: Effects on intestine morphology and targeted intestinal coliforms and clostridium","volume":"11","author":"Kim","year":"2017","journal-title":"Animal"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/8\/2269\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:38:31Z","timestamp":1760164711000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/8\/2269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,31]]},"references-count":35,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2021,8]]}},"alternative-id":["ani11082269"],"URL":"https:\/\/doi.org\/10.3390\/ani11082269","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,31]]}}}