{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:22:58Z","timestamp":1787242978196,"version":"build-2736575974"},"reference-count":29,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T00:00:00Z","timestamp":1628035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004442","name":"NCN","doi-asserted-by":"publisher","award":["NN 311 55 8840"],"award-info":[{"award-number":["NN 311 55 8840"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>A negative energy balance alters the concentration of various fractions of cows\u2019 milk. Therefore, by analyzing the concentration of individual proteins in milk, it is possible to indirectly monitor the health of the animal. The aim of the present study was to identify the relationship between the concentrations of blood \u03b2-hydroxybutyric acid (BHBA) and whey proteins in milk as a marker for the diagnosis of metabolic diseases. The analysis included milk and blood samples from 95 Holstein-Friesian cows, which were divided into three groups that were differentiated in terms of serum BHBA levels 5\u20137 days post-calving: LBHBA, low level of BHBA: 0.200\u20130.500 mmol\/L; NBHBA, optimal level of BHBA- control group: 0.500\u20131.200 mmol\/L; HBHBA, high level of BHBA: &gt;1.200 mmol\/L. Concentrations of \u03b1-lactoalbumin in the milk after 7 days of lactation proceeded in accordance with the concentration of \u03b2-hydroxybutyric acid, as follows: LBHBA &gt; NBHBA &gt; HBHBA. Concentrations of \u03b2-lactoglobulin in milk after 14 days of lactation proceeded in accordance with the concentration of \u03b2-hydroxybutyric acid, as follows: LBHBA &lt; NBHBA &lt; HBHBA. Therefore, it can be concluded that whey proteins can be used as non-invasive markers for diagnosing metabolic diseases. A high concentration of \u03b2-lactoglobulin can be a marker for diagnosing ketosis. Conversely, elevated levels of \u03b1-lactalbumin may indicate the occurrence of a metabolic disorder, such as acidosis.<\/jats:p>","DOI":"10.3390\/ani11082298","type":"journal-article","created":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T08:47:52Z","timestamp":1628066872000},"page":"2298","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Using the Relationship between Concentrations of Selected Whey Proteins and BHBA to Characterize the Metabolism of Dairy Cows in Early Lactation"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2909-2087","authenticated-orcid":false,"given":"Kamila","family":"Puppel","sequence":"first","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrycja","family":"Staniszewska","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Go\u0142\u0119biewski","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Sl\u00f3sarz","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz","family":"Grodkowski","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7683-4998","authenticated-orcid":false,"given":"Pawe\u0142","family":"Solarczyk","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0142gorzata","family":"Kunowska-Sl\u00f3sarz","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Kostusiak","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9093-9905","authenticated-orcid":false,"given":"Beata","family":"Kuczy\u0144ska","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Przysucha","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4321","DOI":"10.1002\/jsfa.7779","article-title":"Metabolic profiles of cow\u2019s blood; a review","volume":"96","author":"Puppel","year":"2016","journal-title":"J. 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