{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T23:19:12Z","timestamp":1785194352583,"version":"3.55.0"},"reference-count":49,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T00:00:00Z","timestamp":1629936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2017-89017-R"],"award-info":[{"award-number":["AGL2017-89017-R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>To date, the underlying mechanisms by which cAMP modulators act during in vitro maturation to improve oocyte developmental competence are poorly understood. Here, we sought to fill this knowledge gap by evaluating the use of phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and adenylyl cyclase activator forskolin during a culture period of 2 h before in vitro maturation (pre-IVM) on the nuclear and cytoplasmic maturation features in essential organelles, cumulus cells activity, and in vitro developmental potential of sheep oocytes. Results showed that pre-IVM treatment significantly decreased (p &lt; 0.05) the DNA damage of mature oocytes (pre-IVM = 2.08% \u00b1 3.51% vs. control = 20.58% \u00b1 3.51%) and increased (p \u2264 0.05) expanded blastocyst rates compared to the control (from the total of oocytes: pre-IVM = 23.89% \u00b1 1.47% vs. control = 18.22% \u00b1 1.47%, and from the cleaved embryos: pre-IVM = 45.16% \u00b1 1.73% vs. control = 32.88% \u00b1 1.73%). Considering that oocytes are highly vulnerable to the accumulation of DNA damage because of exposure to in vitro culture conditions, our results suggest that the modulation of intra-oocyte cAMP levels with forskolin and IBMX before IVM might afford oocytes a more effective DNA repair mechanism to overcome damage obstacles and ultimately improve developmental competence. This previously unappreciated action of cAMP modulators could help to develop improved methods for assisted reproduction technologies in animal and clinical research.<\/jats:p>","DOI":"10.3390\/ani11092512","type":"journal-article","created":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T09:53:23Z","timestamp":1630058003000},"page":"2512","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6942-0829","authenticated-orcid":false,"given":"Daniela-Alejandra","family":"Medina-Ch\u00e1vez","sequence":"first","affiliation":[{"name":"SaBio IREC (CSIC-UCLM-JCCM), ETSIAM, Campus Universitario s\/n, 02071 Albacete, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8232-3940","authenticated-orcid":false,"given":"Irene","family":"S\u00e1nchez-Ajofr\u00edn","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC-UCLM-JCCM), ETSIAM, Campus Universitario s\/n, 02071 Albacete, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6266-0635","authenticated-orcid":false,"given":"Patricia","family":"Peris-Frau","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC-UCLM-JCCM), ETSIAM, Campus Universitario s\/n, 02071 Albacete, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Maside","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC-UCLM-JCCM), ETSIAM, Campus Universitario s\/n, 02071 Albacete, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7975-669X","authenticated-orcid":false,"given":"Vidal","family":"Montoro","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC-UCLM-JCCM), ETSIAM, Campus Universitario s\/n, 02071 Albacete, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roc\u00edo","family":"Fern\u00e1ndez-Santos","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC-UCLM-JCCM), ETSIAM, Campus Universitario s\/n, 02071 Albacete, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3667-6518","authenticated-orcid":false,"given":"Jos\u00e9 Juli\u00e1n","family":"Garde","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC-UCLM-JCCM), ETSIAM, Campus Universitario s\/n, 02071 Albacete, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6429-5157","authenticated-orcid":false,"given":"Ana Josefa","family":"Soler","sequence":"additional","affiliation":[{"name":"SaBio IREC (CSIC-UCLM-JCCM), ETSIAM, Campus Universitario s\/n, 02071 Albacete, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"619902","DOI":"10.3389\/fcell.2020.619902","article-title":"Senescence and apoptosis during in vitro embryo development in a bovine model","volume":"8","author":"Gimeno","year":"2020","journal-title":"Front. 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