{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T06:53:15Z","timestamp":1773471195514,"version":"3.50.1"},"reference-count":40,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T00:00:00Z","timestamp":1630281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study investigated the effects of using mushroom waste compost as the residue medium for Pleurotus eryngii planting, which was used as a feed replacement; its consequent influence on broiler chickens\u2019 intestinal microbiota, anti-inflammatory responses, and anti-oxidative status was likewise studied. A total of 240 male broilers were used and allocated to four treatment groups: the basal diet\u2014control group (corn\u2013soybean); 5% replacement of a soybean meal via PWMC (Pennisetum purpureum Schum No. 2 waste mushroom compost); 5% replacement of a soybean meal via FPW (Saccharomyces cerevisiae fermented PWMC); 5% replacement of a soybean meal via PP (Pennisetum purpureum Schum No. 2). Each treatment had three replicates and 20 birds per pen. The levels of glutathione peroxidase and superoxide dismutase mRNA as well as protein increased in the liver and serum in chickens, respectively; mRNA levels of inflammation-related genes were also suppressed 2 to 10 times in all treatments as compared to those in the control group. The tight junction and mucin were enhanced 2 to 10 times in all treatment groups as compared to those in the control, especially in the PWMC group. Nevertheless, the appetite-related mRNA levels were increased in the PWMC and FPW groups by at least two times. In ileum and cecum, the Firmicutes\/Bacteroidetes ratios in broilers were decreased in the PWMC, FPW, and PP groups. The Lactobacillaceae in the ileum were increased mainly in the PWMC and control groups. Overall, high-fiber feeds (PWMC, FPW, and PP) could enhance the broilers\u2019 health by improving their antioxidant capacities and decreasing their inflammatory response as compared to the control. Based on the results, a 5% replacement of the soybean meal via PWMC is recommended in the broiler chickens\u2019 diet.<\/jats:p>","DOI":"10.3390\/ani11092550","type":"journal-article","created":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T11:01:37Z","timestamp":1630321297000},"page":"2550","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Intestinal Microbiota, Anti-Inflammatory, and Anti-Oxidative Status of Broiler Chickens Fed Diets Containing Mushroom Waste Compost By-Products"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5282-5878","authenticated-orcid":false,"given":"Wen Yang","family":"Chuang","sequence":"first","affiliation":[{"name":"Department of Animal Science, National Chung Hsing University, Taichung 404, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7914-9235","authenticated-orcid":false,"given":"Li Jen","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin Der","family":"Shih","sequence":"additional","affiliation":[{"name":"Taiwan Agricultural Research Institute, Council of Agriculture, Executive Yuan, Taichung 413, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yih Min","family":"Shy","sequence":"additional","affiliation":[{"name":"Hsinchu Branch, Livestock Research Institute, Council of Agriculture, Miaoli 368, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shang Chang","family":"Chang","sequence":"additional","affiliation":[{"name":"Kaohsiung Animal Propagation Station, Livestock Research Institute, Council of Agriculture, Pingtung 912, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0063-5714","authenticated-orcid":false,"given":"Tzu Tai","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Animal Science, National Chung Hsing University, Taichung 404, Taiwan"},{"name":"The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11996","DOI":"10.1073\/pnas.1402183111","article-title":"Land, irrigation water, greenhouse gas, and reactive nitrogen burdens of meat, eggs, and dairy production in the United States","volume":"111","author":"Eshel","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.procbio.2019.01.018","article-title":"Agricultural by-product suitability for the production of chitinous composites and nanofibers utilising Trametes versicolor and Polyporus brumalis mycelial growth","volume":"80","author":"Jones","year":"2019","journal-title":"Process Biochem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1080\/1828051X.2017.1299597","article-title":"Effects of solid-state fermented wheat bran by Bacillus amyloliquefaciens and Saccharomyces cerevisiae on growth performance and intestinal microbiota in broiler chickens","volume":"16","author":"Teng","year":"2017","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1080\/00071668.2017.1280772","article-title":"Antioxidant molecular targets of wheat bran fermented by white rot fungi and its potential modulation of antioxidative status in broiler chickens","volume":"58","author":"Wang","year":"2017","journal-title":"Br. Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1080\/09712119.2018.1480487","article-title":"Effects of substitution of Bermuda grass hay with Trichoderma fermented rice straw on growth, blood, and rumen fluid parameters in Barbados sheep","volume":"46","author":"Pan","year":"2018","journal-title":"J. Appl. Anim. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"8145","DOI":"10.1093\/jn\/127.5.814S","article-title":"Plant limitations to fiber digestion and utilization","volume":"127","author":"Buxton","year":"1997","journal-title":"J. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1016\/j.cell.2016.10.043","article-title":"A Dietary fiber-deprived gut microbiota degrades the colonic mucus barrier and enhances pathogen susceptibility","volume":"167","author":"Desai","year":"2016","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.chom.2017.11.003","article-title":"Fiber-mediated nourishment of gut microbiota protects against diet-induced obesity by restoring IL-22-mediated colonic health","volume":"23","author":"Zou","year":"2018","journal-title":"Cell Host Microbe"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"309","DOI":"10.5713\/ajas.18.0538","article-title":"Potential crosstalk of oxidative stress and immune response in poultry through phytochemicals\u2014A review","volume":"32","author":"Lee","year":"2019","journal-title":"Asian Aust. J. Anim. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.japr.2019.10.009","article-title":"Modified-dietary fiber from cassava pulp reduces abdominal fat and meat cholesterol contents without affecting growth performance of broiler chickens","volume":"29","author":"Okrathok","year":"2020","journal-title":"J. Appl. Poult. Res."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Chuang, W.Y., Lin, W.C., Hsieh, Y.C., Huang, C.M., Chang, S.C., and Lee, T.T. (2019). Evaluation of the combined use of saccharomyces cerevisiae and aspergillus oryzae with phytase fermentation products on growth, inflammatory, and intestinal morphology in broilers. Animals, 9.","DOI":"10.3390\/ani9121051"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"617","DOI":"10.5713\/ajas.17.0657","article-title":"Immunomodulatory effects of phytogenics in chickens and pigs\u2014A review","volume":"31","author":"Huang","year":"2018","journal-title":"Asian Aust. J. Anim. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.3382\/ps\/pev014","article-title":"Botanical alternatives to antibiotics for use in organic poultry production","volume":"94","author":"Biswas","year":"2015","journal-title":"Poult. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Lin, W.C., and Lee, T.T. (2020). Effects of Laetiporus sulphureus-fermented wheat bran on growth performance, intestinal microbiota and digesta characteristics in broiler chickens. Animals, 10.","DOI":"10.3390\/ani10091457"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.livsci.2018.04.012","article-title":"Effects of non-starch polysaccharides in semi-purified diets on performance, serum metabolites, gastrointestinal morphology, and microbial population of male broiler chickens","volume":"214","author":"Kermanshahi","year":"2018","journal-title":"Livest. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"299","DOI":"10.5713\/ajas.16.0438","article-title":"Antioxidant capacity of phytochemicals and their potential effects on oxidative status in animals\u2014A review","volume":"30","author":"Lee","year":"2017","journal-title":"Asian Aust. J. Anim. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Lin, W.C., and Lee, T.T. (2021). Laetiporus sulphureus fermented wheat bran enhanced the antioxidant status, intestinal tight junction, and morphology of broiler chickens. Animals, 11.","DOI":"10.3390\/ani11010149"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"393","DOI":"10.5713\/ajas.20.0223","article-title":"Effects of dietary supplementation with Taiwanese tea byproducts and probiotics on growth performance, lipid metabolism, and the immune response in red feather native chickens","volume":"34","author":"Chen","year":"2021","journal-title":"Anim. Biosci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/j.chom.2015.03.006","article-title":"Effects of diurnal variation of gut microbes and high-fat feeding on host circadian clock function and metabolism","volume":"17","author":"Leone","year":"2015","journal-title":"Cell Host Microbe"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1016\/j.chom.2019.05.001","article-title":"Dynamic modulation of the gut microbiota and metabolome by bacteriophages in a mouse model","volume":"25","author":"Hsu","year":"2019","journal-title":"Cell Host Microbe"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1111\/apt.13248","article-title":"Review article: Dietary fiber\u2013microbiota interactions","volume":"42","author":"Simpson","year":"2015","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_22","unstructured":"AOAC International (2012). Official Methods of Analysis, Association of Official Analytical Chemists. [18th ed.]."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Nielsen, S.S. (2010). Phenol-sulfuric acid method for total carbohydrates. Food Analysis Laboratory Manual, Springer.","DOI":"10.1007\/978-1-4419-1463-7_6"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1016\/j.foodchem.2008.08.013","article-title":"Comparative antioxidant capacities of phenolic compounds measured by various tests","volume":"113","author":"Tabart","year":"2009","journal-title":"Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"307","DOI":"10.5264\/eiyogakuzashi.44.307","article-title":"Studies on product of browning reaction prepared from glucose amine","volume":"44","author":"Oyaizu","year":"1986","journal-title":"Jpn. J. Nutr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"9412767","DOI":"10.1155\/2016\/9412767","article-title":"A simple spectrophotometric method for the determination of thiobarbituric acid reactive substances in fried fast foods","volume":"2016","author":"Zeb","year":"2016","journal-title":"J. Anal. Methods Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"40","DOI":"10.5296\/jfs.v3i1.4995","article-title":"A Comparative study of the antioxidant properties of three pineapple (Ananas Comosus L.) varieties","volume":"3","author":"Yuris","year":"2014","journal-title":"J. Food Stud."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2001","journal-title":"Nat. Protocols"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.tifs.2018.03.009","article-title":"Postbiotics: An evolving term within the functional foods field","volume":"75","author":"Garcia","year":"2018","journal-title":"Trends Food Sci. Tech."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"454","DOI":"10.3382\/ps.2012-02584","article-title":"Effect of increasing levels of bioflavonoids in broiler feed on plasma anti-oxidative potential, lipid metabolites, and fatty acid composition of meat","volume":"92","author":"Kamboh","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1152\/physrev.00031.2007","article-title":"Exercise-induced oxidative stress: Cellular mechanisms and impact on muscle force production","volume":"88","author":"Powers","year":"2007","journal-title":"Physiol. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Bognar, E., Sarszegi, Z., Szabo, A., Debreceni, B., Kalman, N., Tucsek, Z., Sumegi, B., and Gallyas, F. (2013). Antioxidant and anti-inflammatory effects in RAW264.7 macrophages of malvidin, a major red wine polyphenol. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0065355"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"313","DOI":"10.3389\/fnins.2014.00313","article-title":"CART in the regulation of appetite and energy homeostasis","volume":"8","author":"Lau","year":"2014","journal-title":"Front. Neurosci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.neulet.2017.04.064","article-title":"The central effects of alpha-melanocyte stimulating hormone (-MSH) in chicks involve changes in gene expression of neuropeptide Y and other factors in distinct hypothalamic nuclei","volume":"651","author":"Delp","year":"2017","journal-title":"Neurosci. Lett."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1210\/en.2015-1634","article-title":"Identification of the long-sought leptin in chicken and duck: Expression pattern of the highly GC-Rich avian leptin fitsan autocrine\/paracrine rather than endocrine function","volume":"157","author":"Seroussi","year":"2016","journal-title":"Endocrinology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"104440","DOI":"10.1016\/j.appet.2019.104440","article-title":"Control-theory models of body-weight regulation and body-weight regulatory appetite","volume":"144","author":"Geary","year":"2020","journal-title":"Appetite"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1016\/j.ijbiomac.2019.11.137","article-title":"Inulin improves the egg production performance and affects the cecum microbiota of laying hens","volume":"155","author":"Shang","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1002\/jobm.201500726","article-title":"Acute dextran sulfate sodium (DSS)-induced colitis promotes gut microbial dysbiosis in mice","volume":"56","author":"Munyaka","year":"2016","journal-title":"J. Basic Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"6668","DOI":"10.3382\/ps\/pez537","article-title":"Administration of dietary prebiotics improves growth performance and reduces pathogen colonization in broiler chickens","volume":"98","author":"Froebel","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_40","first-page":"109033","article-title":"Development of a microencapsulated synbiotic product and its application in yoghurt","volume":"122","author":"Li","year":"2020","journal-title":"Food Sci. Tech."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/9\/2550\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:55:41Z","timestamp":1760165741000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/9\/2550"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,30]]},"references-count":40,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2021,9]]}},"alternative-id":["ani11092550"],"URL":"https:\/\/doi.org\/10.3390\/ani11092550","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,30]]}}}