{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T23:14:17Z","timestamp":1770678857641,"version":"3.49.0"},"reference-count":32,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T00:00:00Z","timestamp":1632441600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2016M590977"],"award-info":[{"award-number":["2016M590977"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shaanxi Key Research and Development Program","award":["2018ZDXM-NY-046"],"award-info":[{"award-number":["2018ZDXM-NY-046"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFD0501700"],"award-info":[{"award-number":["2018YFD0501700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Modern Agricultural Industry Technology System","award":["CARS-37"],"award-info":[{"award-number":["CARS-37"]}]},{"name":"Technology Innovation and Achievement Transformation for the Demonstration Station of Northwest A&amp;F University","award":["TGZX2019-22"],"award-info":[{"award-number":["TGZX2019-22"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>FATP1 plays an important role in the regulation of fatty acid metabolism and lipid accumulation. In this study, we investigated the patterns of FATP1 expression in various tissues obtained from calf and adult Qinchuan cattle, and in differentiating adipocytes. Next, we investigated the effect of FATP1 expression on preadipocyte differentiation in Qinchuan cattle using overexpression and interference assays. We also identified the differentially expressed genes (DEGs) and pathways associated with FATP1 overexpression\/interference. Our results reveal that FATP1 was broadly expressed in heart, kidney, muscle, small intestine, large intestine, and perirenal fat tissues. While FATP1 overexpression promoted preadipocyte differentiation, fat deposition, and the expression of several genes involved in fat metabolism, FATP1 interference had the opposite effects on adipocyte differentiation. Following FATP1 overexpression and FATP1 interference in adipocytes, RNA-seq analysis was performed to identify DEGs related to fat metabolism. The DEGs identified include SLPI, STC1, SEMA6A, TNFRSF19, SLN, PTGS2, ADCYP1, FADS2, and SCD. Pathway analysis revealed that the DEGs were enriched in the PPAR signaling pathway, AMPK signal pathway, and Insulin signaling pathway. Our results provide an in-depth understanding of the function and regulation mechanism of FAPT1 in fat metabolism.<\/jats:p>","DOI":"10.3390\/ani11102789","type":"journal-article","created":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T20:15:32Z","timestamp":1632514532000},"page":"2789","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Effect of FATP1 on Adipocyte Differentiation in Qinchuan Beef Cattle"],"prefix":"10.3390","volume":"11","author":[{"given":"Xuchun","family":"Liu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shijun","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyun","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujuan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linsheng","family":"Gui","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linsen","family":"Zan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunping","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nature21380","article-title":"Eprs is a critical mtorc1-S6k1 effector that influences adiposity in mice","volume":"542","author":"Arif","year":"2017","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1007\/s11010-021-04057-w","article-title":"The Role of Fatp1 in Lipid Accumulation: A Review","volume":"476","author":"Huang","year":"2021","journal-title":"Mol. Cell Biochem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1006\/geno.2000.6191","article-title":"The Human fatty acid transport protein-1 (SLC27A1; FATP-1) Cdna and gene: Organization, chromosomal localization, and expression","volume":"66","author":"Martin","year":"2000","journal-title":"Genomics"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/0092-8674(94)90252-6","article-title":"Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein","volume":"79","author":"Schaffer","year":"1994","journal-title":"Cell"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"18210","DOI":"10.1074\/jbc.273.29.18210","article-title":"Disruption of the saccharomyces cerevisiae fat1 gene decreases very long-chain fatty acyl-coa synthetase activity and elevates intracellular very long-chain fatty acid concentrations","volume":"273","author":"Watkins","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"16829","DOI":"10.1074\/jbc.M409598200","article-title":"Comparative biochemical studies of the murine fatty acid transport proteins (fatp) expressed in yeast","volume":"280","author":"DiRusso","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1380","DOI":"10.1194\/jlr.M200130-JLR200","article-title":"Fatp1 channels exogenous Fa into 1,2,3-Triacyl-Sn-Glycerol and down-regulates sphingomyelin and cholesterol metabolism in growing 293 cells","volume":"43","author":"Hatch","year":"2002","journal-title":"J. Lipid Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1194\/jlr.M600441-JLR200","article-title":"Fatty acid metabolism in adipocytes: Functional analysis of fatty acid transport proteins 1 and 4","volume":"48","author":"Lobo","year":"2007","journal-title":"J. Lipid Res."},{"key":"ref_9","first-page":"2730","article-title":"Fatty acid transport protein 1: Expression pattern in different tissues of rongchang pigs and correlation with intramuscular fat deposition","volume":"26","author":"Qi","year":"2014","journal-title":"Chin. J. Anim. Nutr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1111\/asj.12701","article-title":"Effects of fatty acid transport protein 1 on proliferation and differentiation of porcine intramuscular preadipocytes","volume":"88","author":"Chen","year":"2017","journal-title":"Anim. Sci. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2907","DOI":"10.1007\/s11033-012-2306-4","article-title":"Fatp1 silence inhibits the differentiation and induces the apoptosis in chicken preadipocytes","volume":"40","author":"Qi","year":"2013","journal-title":"Mol. Biol. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2044","DOI":"10.2527\/jas.2011-4753","article-title":"Expression of fat deposition and fat removal genes is associated with intramuscular fat content in longissimus dorsi muscle of korean cattle steers1","volume":"90","author":"Jeong","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1159\/000095230","article-title":"Structural and functional characterization of the bovine solute carrier family 27 member 1 (SLC27A1) gene","volume":"115","author":"Roy","year":"2006","journal-title":"Cytogenet. Genome Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.cytogfr.2015.12.001","article-title":"Secretory Leukocyte Protease Inhibitor (Slpi), a Multifunctional Protein in the Host Defense Response","volume":"28","author":"Majewski","year":"2016","journal-title":"Cytokine Growth Factor Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"35","DOI":"10.2337\/db19-0502","article-title":"Fat-specific knockout of mecp2 upregulates slpi to reduce obesity by enhancing browning","volume":"69","author":"Liu","year":"2020","journal-title":"Diabetes"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"14438","DOI":"10.1074\/jbc.M109.052001","article-title":"Tumor necrosis factor receptor superfamily member 19 (tnfrsf19) regulates differentiation fate of human mesenchymal (stromal) stem cells through canonical wnt signaling and C\/Ebp","volume":"285","author":"Qiu","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1016\/S0021-9258(17)42256-3","article-title":"Pituitary adenylate cyclase activating polypeptide is an extraordinarily potent intra-pancreatic regulator of insulin secretion from islet beta-cells","volume":"269","author":"Yada","year":"1994","journal-title":"J. Biol. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1210\/mend.15.10.0705","article-title":"Targeted disruption of the pituitary adenylate cyclase-activating polypeptide gene results in early postnatal death associated with dysfunction of lipid and carbohydrate metabolism","volume":"15","author":"Gray","year":"2001","journal-title":"Mol. Endocrinol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1254\/jphs.FP0072173","article-title":"Markedly reduced white adipose tissue and increased insulin sensitivity in adcyap1-deficient mice","volume":"107","author":"Tomimoto","year":"2008","journal-title":"J. Pharmacol. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"103967","DOI":"10.1016\/j.jprot.2020.103967","article-title":"Integrated transcriptome and phosphoproteome analyses reveal that fads2 is critical for maintaining body lc-pufa homeostasis","volume":"229","author":"Zhao","year":"2020","journal-title":"J. Proteom."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"547","DOI":"10.5194\/aab-62-547-2019","article-title":"The effect of polymorphism in the fads2 gene on the fatty acid composition of bovine milk","volume":"62","author":"Proskura","year":"2019","journal-title":"Arch. Anim. Breed"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Li, M., Lu, X., Gao, Q., Wang, M., Arbab, A.A.I., Sun, Y., Chen, Z., Zhang, H., Karrow, N.A., and Yang, Z. (2019). A functional 3\u2032 utr polymorphism of fads2 affects cow milk composition through modifying mir-744 binding. Animals, 9.","DOI":"10.3390\/ani9121090"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1002\/oby.21035","article-title":"Polyunsaturated fatty acid regulation of adipocyte fads1 and fads2 expression and function","volume":"23","author":"Ralston","year":"2015","journal-title":"Obesity"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"107806","DOI":"10.1016\/j.celrep.2020.107806","article-title":"Mtor signaling and srebp activity increase fads2 expression and can activate sapienate biosynthesis","volume":"31","author":"Triki","year":"2020","journal-title":"Cell Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"40024","DOI":"10.1038\/srep40024","article-title":"Regulation of fads2 transcription by srebp-1 and ppar-\u03b1 influences lc-pufa biosynthesis in fish","volume":"7","author":"Dong","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2462","DOI":"10.1073\/pnas.1914553117","article-title":"Scd1 controls de novo beige fat biogenesis through succinate-dependent regulation of mitochondrial complex Ii","volume":"117","author":"Liu","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1194\/jlr.RA120000869","article-title":"Scd1 promotes lipid mobilization in subcutaneous white adipose tissue","volume":"61","author":"Zou","year":"2020","journal-title":"J. Lipid Res."},{"key":"ref_28","first-page":"1060","article-title":"Lipoprotein lipase. A multifunctional enzyme relevant to common metabolic diseases","volume":"320","author":"Eckel","year":"1989","journal-title":"N. Engl. J. Med."},{"key":"ref_29","first-page":"D388","article-title":"Lipoprotein lipase: Physiology, biochemistry, and molecular biology","volume":"6","author":"Goldberg","year":"2001","journal-title":"Front. Biosci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"9030","DOI":"10.1016\/S0021-9258(18)81898-1","article-title":"Insulin regulation of lipoprotein lipase activity in 3t3-L1 Adipocytes is mediated at posttranscriptional and posttranslational levels","volume":"264","author":"Semenkovich","year":"1989","journal-title":"J. Biol. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1743-7075-4-22","article-title":"Role of adipocyte-derived lipoprotein lipase in adipocyte hypertrophy","volume":"4","author":"Gonzales","year":"2007","journal-title":"Nutr. Metab."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1038\/s41418-019-0453-z","article-title":"Tumor suppressor Zhx2 inhibits nafld-hcc progression via blocking lpl-mediated lipid uptake","volume":"27","author":"Wu","year":"2020","journal-title":"Cell Death Differ."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/10\/2789\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:04:31Z","timestamp":1760166271000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/10\/2789"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,24]]},"references-count":32,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["ani11102789"],"URL":"https:\/\/doi.org\/10.3390\/ani11102789","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,24]]}}}